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- PDB-8tw3: hMPV fusion protein complexed with single domain antibodies sdHMP... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8tw3 | |||||||||
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Title | hMPV fusion protein complexed with single domain antibodies sdHMPV16 and sdHMPV12 | |||||||||
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![]() | VIRAL PROTEIN/IMMUNE SYSTEM / metapneumovirus / fusion protein / VIRAL PROTEIN / VIRAL PROTEIN-Immune System complex | |||||||||
Function / homology | Precursor fusion glycoprotein F0, Paramyxoviridae / Fusion glycoprotein F0 / fusion of virus membrane with host plasma membrane / host cell plasma membrane / virion membrane / plasma membrane / Fusion glycoprotein F0![]() | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Rush, S.A. / McLellan, J.S. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: A neutralizing single-domain antibody that targets the trimer interface of the human metapneumovirus fusion protein. Authors: Ballegeer, M. / van Scherpenzeel, R.C. / Delgado, T. / Iglesias-Caballero, M. / Garcia Barreno, B. / Pandey, S. / Rush, S.A. / Kolkman, J.A. / Mas, V. / McLellan, J.S. / Saelens, X. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 458.4 KB | Display | ![]() |
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PDB format | ![]() | 374.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 495.3 KB | Display | ![]() |
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Full document | ![]() | 500.5 KB | Display | |
Data in XML | ![]() | 41.1 KB | Display | |
Data in CIF | ![]() | 56.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 20633.838 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Antibody | Mass: 20863.871 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Protein | Mass: 58805.473 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() #4: Sugar | ChemComp-NAG / Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | Temperature: 293 K / Method: vapor diffusion / pH: 7 / Details: 0.1M HEPES and 18% PEG 12,000 |
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-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Dec 2, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 2.9→44.97 Å / Num. obs: 32688 / % possible obs: 98.6 % / Redundancy: 5.6 % / CC1/2: 0.962 / Rmerge(I) obs: 0.214 / Rpim(I) all: 0.099 / Rrim(I) all: 0.237 / Χ2: 1.04 / Net I/σ(I): 7 / Num. measured all: 181517 |
Reflection shell | Resolution: 2.9→3.06 Å / % possible obs: 99.3 % / Redundancy: 5.7 % / Rmerge(I) obs: 0.862 / Num. measured all: 27228 / Num. unique obs: 4780 / CC1/2: 0.638 / Rpim(I) all: 0.393 / Rrim(I) all: 0.951 / Χ2: 0.95 / Net I/σ(I) obs: 2.4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.9→44.97 Å
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Refine LS restraints |
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LS refinement shell |
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