NKG2-DtypeIIintegralmembraneprotein / Killer cell lectin-like receptor subfamily K member 1 / NK cell receptor D / NKG2-D-activating NK receptor
Mass: 30432.443 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: KLRK1, KLRK1, D12S2489E, NKG2D / Production host: Homo sapiens (human) / References: UniProt: P26718
#2: Protein
MHCclassIpolypeptide-relatedsequenceA
Mass: 22144.814 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: MICA / Production host: Homo sapiens (human) / References: UniProt: Q29983
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.97741 Å / Relative weight: 1
Reflection
Resolution: 3.83→70.02 Å / Num. obs: 4537 / % possible obs: 86.3 % / Redundancy: 2.8 % / CC1/2: 0.758 / Net I/σ(I): 10.8
Reflection shell
Diffraction-ID: 1
Resolution (Å)
Redundancy (%)
Rmerge(I) obs
Num. unique obs
CC1/2
CC star
Rpim(I) all
Rrim(I) all
Χ2
% possible all
4-4.07
2.7
0.722
231
0.758
0.929
0.476
0.87
2.485
88.2
4.07-4.14
2.8
0.609
222
0.818
0.949
0.402
0.733
2.519
89.5
4.14-4.22
2.7
0.479
223
0.897
0.972
0.324
0.581
2.469
89.6
4.22-4.31
2.7
0.501
235
0.841
0.956
0.326
0.601
2.699
88
4.31-4.4
2.7
0.267
222
0.914
0.977
0.182
0.326
2.669
87.4
4.4-4.5
2.7
0.284
223
0.907
0.975
0.193
0.346
3.277
89.2
4.5-4.62
2.8
0.339
215
0.947
0.986
0.226
0.409
2.672
85
4.62-4.74
2.7
0.291
227
0.935
0.983
0.192
0.35
2.454
86.3
4.74-4.88
2.9
0.296
219
0.918
0.978
0.193
0.356
2.629
87.3
4.88-5.04
2.8
0.279
225
0.912
0.977
0.186
0.337
2.509
87.9
5.04-5.22
2.8
0.234
235
0.93
0.982
0.152
0.281
2.154
88.3
5.22-5.43
2.8
0.256
216
0.929
0.981
0.169
0.309
2.337
87.1
5.43-5.67
2.8
0.223
238
0.952
0.988
0.147
0.268
2.157
87.2
5.67-5.97
2.9
0.215
224
0.923
0.98
0.144
0.261
2.277
87.5
5.97-6.35
2.9
0.206
230
0.941
0.985
0.136
0.248
2.138
85.2
6.35-6.84
2.8
0.179
231
0.973
0.993
0.12
0.217
1.824
88.5
6.84-7.52
2.9
0.126
228
0.983
0.996
0.083
0.152
1.888
85.4
7.52-8.6
2.9
0.096
227
0.981
0.995
0.065
0.117
1.743
84.4
8.6-10.82
2.8
0.06
224
0.99
0.997
0.043
0.075
1.422
79.7
10.82-50
2.8
0.05
242
0.993
0.998
0.035
0.061
1.141
77.6
-
Processing
Software
Name
Version
Classification
REFMAC
5.8.0155
refinement
HKL-2000
datascaling
HKL-2000
datareduction
PHASER
phasing
Refinement
Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.83→70.02 Å / Cor.coef. Fo:Fc: 0.883 / Cor.coef. Fo:Fc free: 0.722 / SU B: 188.232 / SU ML: 1.045 / Cross valid method: THROUGHOUT / ESU R Free: 1.33 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.3618
233
5.2 %
RANDOM
Rwork
0.26948
-
-
-
obs
0.27392
4290
81.06 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK