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Open data
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Basic information
Entry | Database: PDB / ID: 8tly | ||||||||||||
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Title | Human ASCC1 Phosphodiesterase/Ligase Domain | ||||||||||||
![]() | Activating signal cointegrator 1 complex subunit 1 | ||||||||||||
![]() | RNA BINDING PROTEIN / alkylation response / RNA damage / KH domain / phosphoesterase domain / RNA ligase domain | ||||||||||||
Function / homology | ![]() ALKBH3 mediated reversal of alkylation damage / DNA alkylation repair / DNA repair complex / DNA duplex unwinding / neuromuscular junction / transcription regulator complex / nuclear speck / regulation of DNA-templated transcription / RNA binding / nucleoplasm / nucleus Similarity search - Function | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Tsutakawa, S.E. / Tainer, J.A. / Arvai, A.S. / Thapar, R. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: ASCC1 structures and bioinformatics reveal a novel helix-clasp-helix RNA-binding motif linked to a two-histidine phosphodiesterase. Authors: Chinnam, N.B. / Thapar, R. / Arvai, A.S. / Sarker, A.H. / Soll, J.M. / Paul, T. / Syed, A. / Rosenberg, D.J. / Hammel, M. / Bacolla, A. / Katsonis, P. / Asthana, A. / Tsai, M.S. / Ivanov, I. ...Authors: Chinnam, N.B. / Thapar, R. / Arvai, A.S. / Sarker, A.H. / Soll, J.M. / Paul, T. / Syed, A. / Rosenberg, D.J. / Hammel, M. / Bacolla, A. / Katsonis, P. / Asthana, A. / Tsai, M.S. / Ivanov, I. / Lichtarge, O. / Silverman, R.H. / Mosammaparast, N. / Tsutakawa, S.E. / Tainer, J.A. #1: ![]() Title: Target highlights in CASP14: Analysis of models by structure providers. Authors: Alexander, L.T. / Lepore, R. / Kryshtafovych, A. / Adamopoulos, A. / Alahuhta, M. / Arvin, A.M. / Bomble, Y.J. / Bottcher, B. / Breyton, C. / Chiarini, V. / Chinnam, N.B. / Chiu, W. / ...Authors: Alexander, L.T. / Lepore, R. / Kryshtafovych, A. / Adamopoulos, A. / Alahuhta, M. / Arvin, A.M. / Bomble, Y.J. / Bottcher, B. / Breyton, C. / Chiarini, V. / Chinnam, N.B. / Chiu, W. / Fidelis, K. / Grinter, R. / Gupta, G.D. / Hartmann, M.D. / Hayes, C.S. / Heidebrecht, T. / Ilari, A. / Joachimiak, A. / Kim, Y. / Linares, R. / Lovering, A.L. / Lunin, V.V. / Lupas, A.N. / Makbul, C. / Michalska, K. / Moult, J. / Mukherjee, P.K. / Nutt, W.S. / Oliver, S.L. / Perrakis, A. / Stols, L. / Tainer, J.A. / Topf, M. / Tsutakawa, S.E. / Valdivia-Delgado, M. / Schwede, T. #2: Journal: Methods Mol.Biol. / Year: 2022 Title: Universally Accessible Structural Data on Macromolecular Conformation, Assembly, and Dynamics by Small Angle X-Ray Scattering for DNA Repair Insights. Authors: Chinnam, N.B. / Syed, A. / Burnett, K.H. / Hura, G.L. / Tainer, J.A. / Tsutakawa, S.E. #3: Journal: Methods Enzymol. / Year: 2023 Title: Combining small angle X-ray scattering (SAXS) with protein structure predictions to characterize conformations in solution. Authors: Chinnam, N.B. / Syed, A. / Hura, G.L. / Hammel, M. / Tainer, J.A. / Tsutakawa, S.E. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 217.3 KB | Display | ![]() |
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PDB format | ![]() | 143.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 433.1 KB | Display | ![]() |
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Full document | ![]() | 435.8 KB | Display | |
Data in XML | ![]() | 18.4 KB | Display | |
Data in CIF | ![]() | 25.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8tukC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28124.895 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.48 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 12.5% MPEG 2K, 200 mM imidizole/malate (I/M) pH 5.0, 5% saturated KCl at room temperature. For cryoprotection, 25% ethylene glycol added to the mother liquor Temp details: room temperature |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Feb 19, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97946 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→35.73 Å / Num. obs: 12679 / % possible obs: 99.6 % / Redundancy: 1.72 % / Biso Wilson estimate: 44.18 Å2 / CC1/2: 0.988 / Net I/σ(I): 9.71 |
Reflection shell | Resolution: 2.8→2.97 Å / Num. unique obs: 1990 / CC1/2: 0.796 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 43.77 Å2 | |||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→35.73 Å
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Refine LS restraints |
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LS refinement shell |
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