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- PDB-8tku: ZIG-4-INS-6 complex, primitive monoclinic form -

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Basic information

Entry
Database: PDB / ID: 8tku
TitleZIG-4-INS-6 complex, primitive monoclinic form
Components
  • Probable insulin-like peptide beta-type 5
  • Zwei Ig domain protein zig-4
KeywordsSIGNALING PROTEIN/PROTEIN BINDING / NEUROPEPTIDE / SIGNALING PROTEIN / PROTEIN BINDING / SIGNALING PROTEIN-PROTEIN BINDING complex
Function / homology
Function and homology information


cellular response to salt / negative regulation of dauer entry / dauer larval development / regulation of synaptic assembly at neuromuscular junction / chemosensory behavior / olfactory learning / neuron development / insulin receptor binding / hormone activity / insulin receptor signaling pathway ...cellular response to salt / negative regulation of dauer entry / dauer larval development / regulation of synaptic assembly at neuromuscular junction / chemosensory behavior / olfactory learning / neuron development / insulin receptor binding / hormone activity / insulin receptor signaling pathway / extracellular space / extracellular region
Similarity search - Function
Nematode insulin-like peptide, beta type / : / Nematode insulin-related peptide beta type / : / Insulin, conserved site / Insulin family signature. / Insulin-like superfamily / Immunoglobulin domain / Immunoglobulin I-set / Immunoglobulin I-set domain ...Nematode insulin-like peptide, beta type / : / Nematode insulin-related peptide beta type / : / Insulin, conserved site / Insulin family signature. / Insulin-like superfamily / Immunoglobulin domain / Immunoglobulin I-set / Immunoglobulin I-set domain / Immunoglobulin subtype 2 / Immunoglobulin C-2 Type / Immunoglobulin subtype / Immunoglobulin / Ig-like domain profile. / Immunoglobulin-like domain / Immunoglobulin-like domain superfamily / Immunoglobulin-like fold
Similarity search - Domain/homology
Zwei Ig domain protein zig-4 / Probable insulin-like peptide beta-type 5
Similarity search - Component
Biological speciesCaenorhabditis elegans (invertebrata)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.35 Å
AuthorsCheng, S. / Baltrusaitis, E. / Aziz, Z. / Nawrocka, W.I. / Ozkan, E.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of Neurological Disorders and Stroke (NIH/NINDS)R01NS097161 United States
CitationJournal: Biorxiv / Year: 2024
Title: Nematode Extracellular Protein Interactome Expands Connections between Signaling Pathways.
Authors: Nawrocka, W.I. / Cheng, S. / Hao, B. / Rosen, M.C. / Cortes, E. / Baltrusaitis, E.E. / Aziz, Z. / Kovacs, I.A. / Ozkan, E.
History
DepositionJul 25, 2023Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 28, 2024Provider: repository / Type: Initial release
Revision 1.1Oct 16, 2024Group: Database references / Structure summary
Category: citation / citation_author ...citation / citation_author / pdbx_entry_details / pdbx_modification_feature
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Zwei Ig domain protein zig-4
B: Probable insulin-like peptide beta-type 5
C: Zwei Ig domain protein zig-4
D: Probable insulin-like peptide beta-type 5
E: Zwei Ig domain protein zig-4
F: Probable insulin-like peptide beta-type 5
G: Zwei Ig domain protein zig-4
H: Probable insulin-like peptide beta-type 5


Theoretical massNumber of molelcules
Total (without water)120,7488
Polymers120,7488
Non-polymers00
Water70339
1
A: Zwei Ig domain protein zig-4
B: Probable insulin-like peptide beta-type 5


Theoretical massNumber of molelcules
Total (without water)30,1872
Polymers30,1872
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
C: Zwei Ig domain protein zig-4
D: Probable insulin-like peptide beta-type 5


Theoretical massNumber of molelcules
Total (without water)30,1872
Polymers30,1872
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
E: Zwei Ig domain protein zig-4
F: Probable insulin-like peptide beta-type 5


Theoretical massNumber of molelcules
Total (without water)30,1872
Polymers30,1872
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
G: Zwei Ig domain protein zig-4
H: Probable insulin-like peptide beta-type 5


Theoretical massNumber of molelcules
Total (without water)30,1872
Polymers30,1872
Non-polymers00
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)73.839, 55.739, 149.945
Angle α, β, γ (deg.)90.000, 93.780, 90.000
Int Tables number4
Space group name H-MP1211
Space group name HallP2yb
Symmetry operation#1: x,y,z
#2: -x,y+1/2,-z
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 43 through 150 or resid 154...
d_2ens_1(chain "C" and (resid 43 through 233 or (resid 234...
d_3ens_1(chain "E" and (resid 43 through 233 or (resid 234...
d_4ens_1chain "G"
d_1ens_2(chain "B" and resid 62 through 112)
d_2ens_2(chain "D" and resid 62 through 112)
d_3ens_2(chain "F" and resid 62 through 112)
d_4ens_2(chain "H" and resid 62 through 112)

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11ens_1PROPROGLUGLUAA43 - 1503 - 110
d_12ens_1CYSCYSHISHISAA154 - 249114 - 209
d_21ens_1PROPROHISHISCC43 - 2493 - 209
d_31ens_1PROPROHISHISEE43 - 2493 - 209
d_41ens_1PROPROHISHISGG43 - 2493 - 209
d_11ens_2PROPROPROPROBB62 - 1124 - 54
d_21ens_2PROPROPROPRODD62 - 1124 - 54
d_31ens_2PROPROPROPROFF62 - 1124 - 54
d_41ens_2PROPROPROPROHH62 - 1124 - 54

NCS ensembles :
ID
ens_1
ens_2

NCS oper:
IDCodeMatrixVector
1given(-0.993335918358, 0.0963661180079, -0.0632243987727), (-0.109918109867, -0.957075754114, 0.268186520934), (-0.0346664452093, 0.273348810474, 0.961290104697)45.4907129287, 34.2077528125, 0.00899187516622
2given(0.990295577877, -0.115258876393, 0.0776534600057), (-0.138461332408, -0.866338511784, 0.479881279513), (0.0119636058976, -0.485976310546, -0.873890094761)-5.58547031341, 25.1084079414, 78.3653393089
3given(-0.999965426385, -0.00805582797323, 0.00206147263171), (-0.00711671516513, 0.957327452616, 0.288917809823), (-0.0043009765175, 0.288893149977, -0.957351685379)39.9487228549, -9.53800733938, 69.4017890868
4given(-0.997610670265, 0.0679381988571, -0.0125439910096), (-0.0688113479686, -0.960930694019, 0.268099234768), (0.00616027313849, 0.268321826224, 0.96330963278)44.340168732, 33.780652904, -1.15081259072
5given(0.994042002441, -0.100369576781, 0.0425022992412), (-0.108588122518, -0.878143250381, 0.465910990918), (-0.0094401817716, -0.467750339248, -0.883810218939)-4.60906964325, 25.1092508237, 79.1279371536
6given(-0.99932941638, -0.0111872591378, 0.034864922055), (-0.00108768429732, 0.960831788273, 0.277130098668), (-0.0365996516348, 0.276906337735, -0.96019963842)39.1706154356, -9.41396576138, 69.932825615

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Components

#1: Protein
Zwei Ig domain protein zig-4


Mass: 23689.566 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Caenorhabditis elegans (invertebrata) / Gene: zig-4 / Cell line (production host): High Five cells / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: G5ECB1
#2: Protein
Probable insulin-like peptide beta-type 5 / INS-6


Mass: 6497.392 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Caenorhabditis elegans (invertebrata) / Gene: ins-6 / Cell line (production host): High Five cells / Production host: Trichoplusia ni (cabbage looper) / References: UniProt: P56174
#3: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 39 / Source method: isolated from a natural source / Formula: H2O
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.55 Å3/Da / Density % sol: 51.76 %
Crystal growTemperature: 295 K / Method: vapor diffusion, sitting drop
Details: 0.2 M sodium iodide, 0.1 M bis-tris propane, pH 6.5, 20% PEG 3350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-E / Wavelength: 0.97918 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Oct 25, 2019
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97918 Å / Relative weight: 1
ReflectionResolution: 2.35→200 Å / Num. obs: 50159 / % possible obs: 97.9 % / Redundancy: 3.1 % / Biso Wilson estimate: 66.38 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.062 / Rsym value: 0.062 / Net I/σ(I): 10.7
Reflection shellResolution: 2.35→2.49 Å / Redundancy: 3.1 % / Rmerge(I) obs: 1.248 / Mean I/σ(I) obs: 0.9 / Num. unique obs: 7983 / CC1/2: 0.463 / Rsym value: 1.248 / % possible all: 97.1

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
XDS20190806data reduction
XDS20190806data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.35→74.81 Å / SU ML: 0.4344 / Cross valid method: FREE R-VALUE / Phase error: 31.456
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2398 1977 3.95 %
Rwork0.1966 48119 -
obs0.1983 50096 97.79 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 83.08 Å2
Refinement stepCycle: LAST / Resolution: 2.35→74.81 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7755 0 0 39 7794
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0087895
X-RAY DIFFRACTIONf_angle_d1.001610742
X-RAY DIFFRACTIONf_chiral_restr0.05731247
X-RAY DIFFRACTIONf_plane_restr0.00811396
X-RAY DIFFRACTIONf_dihedral_angle_d16.38432789
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AAX-RAY DIFFRACTIONTorsion NCS0.693744490925
ens_1d_3AAX-RAY DIFFRACTIONTorsion NCS0.662222147987
ens_1d_4AAX-RAY DIFFRACTIONTorsion NCS0.460312800531
ens_2d_2BBX-RAY DIFFRACTIONTorsion NCS1.01833621931
ens_2d_3BBX-RAY DIFFRACTIONTorsion NCS0.924927826132
ens_2d_4BBX-RAY DIFFRACTIONTorsion NCS0.540537020466
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.35-2.410.44331220.38723297X-RAY DIFFRACTION94.66
2.41-2.470.40081600.3543419X-RAY DIFFRACTION98.89
2.47-2.550.37571420.34463459X-RAY DIFFRACTION98.44
2.55-2.630.41221320.34313382X-RAY DIFFRACTION96.62
2.63-2.720.44041370.33173456X-RAY DIFFRACTION99.14
2.72-2.830.31991450.28113429X-RAY DIFFRACTION99.11
2.83-2.960.27661470.23153490X-RAY DIFFRACTION98.8
2.96-3.120.28871430.22553436X-RAY DIFFRACTION98.92
3.12-3.310.29761490.23463458X-RAY DIFFRACTION98.74
3.31-3.570.27081340.20043449X-RAY DIFFRACTION98.25
3.57-3.930.23461340.18793406X-RAY DIFFRACTION96.27
3.93-4.50.19861480.16173424X-RAY DIFFRACTION97.65
4.5-5.660.17891330.14823482X-RAY DIFFRACTION97.15
5.66-74.810.18441510.15953532X-RAY DIFFRACTION96.44
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.188725085317-0.28816345010.04063243743220.543718925991-0.0723651898529-0.005235401115090.0578717415629-0.154439146404-0.0468001535339-0.2701308435910.136189488267-0.525314693474-0.5602163930210.806334979599-0.000180790266860.5894171166830.03850345529120.04378533319460.6352513151420.006316720090970.52105984560219.377357565811.748831304513.5424924593
21.245958530270.962643867879-0.3746749814731.26947019799-1.294071678030.6312333657180.09170332765260.1546598070510.225875000988-0.00555073832558-0.08548019826580.0448996179769.81515937236E-50.4062718350267.75251488026E-50.5837170720410.0613593077256-0.008670421246070.5982537173560.05191072624230.55954144402211.503946599720.249639117813.0387641532
30.4905297485160.221663794405-0.2284797702180.695996795493-0.5785262327620.2416819825550.0357141586861-0.02561445734250.1570085374250.03169261603890.0578341546-0.0686546122691-0.2051862702470.2961838718522.70683091999E-50.5519495375190.0150891694474-0.01246381578480.6263812931640.06896426609290.4724472956512.30293422818.963410348415.1770128459
4-0.0558107957402-0.03465554583030.0455314504829-0.00703793853678-0.2795524419940.4278901041410.1867694577860.5957367968040.185846739833-0.01829202931360.295814512017-0.30931660689-0.492245800990.473361489350.003918547375260.5173039190220.002602311445150.04337101530640.7739741713940.09099242976560.41014066411820.206462490820.92778736453.23381826086
50.011105785360.0849036873601-0.08088320211740.0309331247605-0.1518605835350.1159739718580.0464145424611-0.216416768480.558724653261-0.4193448329960.1493259714540.283465692405-0.3117332794610.0179367301399-0.0004585027959380.8702402710.1084405507870.04378231119050.9223537343440.1845636404530.864588263290.75765662049126.3203456018-3.91418695813
62.233327074140.708952386319-0.8840716529741.27663899880.7181878213641.279177488380.1802523945860.1139250577880.06711554458540.0667676671944-0.0157575609120.3867144283880.00183267115102-0.0147332251375-5.88171960855E-50.4946667153110.03196905283190.03374752814150.4428348668930.03125295686070.52345984950.19193855132714.640598304119.8128976757
70.612106125348-0.1403022169610.1658421063870.08478003863350.06194283685820.232235724610.0372597414835-0.6847906461010.218327127578-0.253282835236-0.0806687738575-0.306452384360.0406510780462-0.060701336175-0.0002674618655570.616670532957-0.04975456378940.04078634670770.665883874185-0.1128454618960.701886028531-9.2019846170313.894214609724.9064501979
80.172389088497-0.138976603402-0.02627401106840.4108339432410.4163700472940.2844130759330.151413274855-0.274127873246-0.135455051585-0.322170184616-0.004951502332630.7784559678850.104527132026-0.03664702078320.0002351667592360.614251846923-0.02771151460210.05128219956220.6101331580630.009681658323950.628106593541-2.797714505667.2211067839228.6844684948
91.59516122540.427955889047-1.247038346161.03330795301-0.00424735306750.7854976122720.127718380214-0.151922554206-0.163134383055-0.1185348748840.2640643141510.3254733107340.254783363146-0.5160437954880.0001683727672540.5100055473950.03249337264960.004652764070330.498003136408-0.01691978073540.545542979415-2.8437333056410.735618203417.6061222724
100.122823673979-0.1989796499650.2638299524571.11840756906-0.9189990065980.7571656528460.0170081989491.28931769713-1.12946052697-0.0252224943112-0.0164794850860.130506603270.303209828015-0.440740457145-0.0002475522688450.6997135123010.0921360707909-0.02795449921930.669813528129-0.0380472211230.6527916405558.48002293853-0.98195937790517.265683077
110.141842322980.104683675561-0.02497133735790.05089658770220.003511907693990.1531700656730.957658506790.4275321208540.4583790734140.200504398514-0.340074632712-0.547109421268-0.01273733913641.102648625630.000576523457641.012601226820.18294693486-0.117620919780.6864878613050.01965844883410.92053086940211.2989988489-8.5310911058618.6873561982
120.28617157688-0.252091574546-0.02234501168650.147608834179-0.03764084265350.1858537075590.122049365005-1.39878103818-0.245000493196-0.172992846163-0.01397299930250.1633853648811.139781676350.791089810081-0.0003243133951560.9842011372260.158812124654-0.08840299372790.8016569704820.03970068428751.0371426021913.6639785203-3.3822899169127.3013018081
131.839680729620.5393660208790.3752467605281.823423233340.4702836396080.9106467977250.06703117671370.09152079088230.0164961123748-0.01175739126550.0518171604034-0.1243417523250.039916784861-0.110852905831-7.06088506927E-50.5256582220420.05125017994420.0103235666820.4483450352410.002587314989750.52312077851933.097856820217.984024497617.0559494233
140.06803655718530.1010431961670.136180829580.02325334765490.1654336867860.1368338874540.7912103474350.624199404711-0.808242482545-0.1805084963920.379566085788-0.981640343290.5556252981220.5572193862430.0007496256232870.8393865204460.15159597069-0.09137967653670.911964188019-0.0969704571231.0211463568247.69181918589.44085479494.77378388572
151.08631437882-0.222145457672-0.9900539038580.8188821308110.01028730754591.182584862370.0808188738671-0.07482834715480.0815935070024-0.104022648679-0.000936569999757-0.510340599022-0.1243866248250.2252285295972.25745946932E-50.4666523846410.0100798465384-0.03808462340290.466420952713-0.01016071422760.6654636563848.43588957726.488250997324.6919155755
161.299527101560.6072132883750.6083267712740.5798232182690.04070338814150.72821078178-0.001349358601790.18290410290.629226326068-0.02658298142870.201687122264-0.724899377372-0.01212811754980.3954316042256.06419729448E-50.553837009721-0.00477162420758-0.03075423474650.4181993392640.00135804549350.70658622121647.654090313130.968170974923.069423533
170.852114915389-0.206932492831-0.2699096482090.6281383110520.5288832495490.8375443844220.04825483996470.7355812111550.250285192407-0.274399011076-0.231994193943-0.320657521856-1.2682992963-0.1339781357040.02961264791591.035695794930.08958728732140.03572673309140.5220459054120.06800260284891.0032533740437.108201823839.648376189615.6209135628
180.1621306466760.00192831211982-0.186384341407-0.0176524259033-0.01744116227340.2495778028481.198092394480.9243577407110.902758655301-0.476982843035-0.2415846846060.607911500706-1.30682796446-0.5920478274380.005520594657811.297042562680.204414243803-0.08685882138270.6817890755190.1262014963931.1651095324932.436951173946.110982093114.9063628537
190.3404403912360.286881799030.4122459829130.3096763498950.3555051870020.516386910818-0.719153204199-0.519228837940.7879135499020.1908686256810.2086641237-0.463479871351-1.00993237935-0.61160049029-0.02311913518360.9838435716080.1565831224350.0764220993830.560400370464-0.0619880821761.1842070483629.309367088243.492106804124.2669957025
202.068791635-0.2947243309350.670351081931.75711838733-0.18972290361-0.06477621994880.110142704453-0.416835395625-0.106038683381-0.120488970806-0.239562601393-0.306257299465-0.244386426010.3585627502010.0003560611928440.612783107107-0.04986166759960.008591705358120.7387517847580.08201011017430.5839611802075.4705605650415.664057321456.4159510244
210.769653297774-0.774980120037-0.5118493391250.688810393780.4955240618530.337721807881-0.160261381035-0.535128868897-0.6478231832710.03982031549530.00144709749863-0.434279355594-0.0219864048377-0.102324007962-8.55979452239E-50.581477822761-0.0629569723867-0.04061719590060.9133243526760.2833785353520.7724981869769.24176914836.4714963359962.0269481725
220.0200341070071-0.05131201552250.08800982312920.08806442611-0.1144793241090.0455910535892-0.0954444477364-0.1865664587370.1226411505770.198046943208-0.1886960571970.234594121060.183881205807-0.4321021983294.48719140294E-50.667505804975-0.161231437704-0.04711104372841.23443789040.2565863249660.826020803158-8.484737421141.8500416629468.2178095558
232.167525420630.81881492394-0.2643508169141.183488241330.07321412846050.257118270607-0.0912975674894-0.3970032958710.477798158335-0.0791514187113-0.07772387797740.327475693669-0.0965806123356-0.4008495191526.10783246053E-50.6034394136830.0635296099742-0.0749536613670.659110221439-0.05299571654640.62563381237-8.1070260218525.301046598952.0421842741
241.77731666539-0.5407877915841.029179966990.7482182691330.1847428984590.844241439429-0.164031352652-1.495958524060.0746205416646-0.0577436850389-0.00646199803588-0.202136201185-0.265595852827-0.306722982043-0.01469999234750.6986414556480.0429810023154-0.03364154962040.772929711404-0.2109613633470.595608855237-8.0642171232524.568588501457.674074053
250.3761546834710.190621172307-0.4050534180.755530625149-0.4843238636490.4627298092180.419328290746-0.6558922525271.300369943770.301680943761-0.6609772941740.162808230257-1.42203735839-0.4661115940620.008229391282381.12426789852-0.006941718771950.07534391570360.881588958086-0.2019770152181.070705119713.3404466355934.174587621963.9471196833
260.118767060308-0.0357060081614-0.08827877237310.01355487079820.003066066926060.2303895081210.351475812774-1.051679094670.880050973278-0.0561461540139-0.479817916195-0.4538087783780.4424374743551.137544745730.002989693773591.5080300359-0.0192561503459-0.2389880015481.11952376212-0.1767717756671.354367309118.1133160611740.013574705366.3686548023
270.484436810060.007520636601160.03411142284090.20165622751-0.08937622765760.02681747947990.2042228810090.8389147666470.06808943523380.365577647392-0.842672814766-0.397280892557-0.897528463237-0.2706949160910.0001788441857341.38200338992-0.157428691672-0.01336054703090.724503878894-0.0818030718461.2401176460610.125303665939.459549779656.4068323668
281.391296821480.11063801013-0.7707062213590.3802562309770.1722895015860.620821750181-0.039423960001-0.740468389219-0.01279120839630.198859506725-0.09793274178120.3038447491150.099060196331-0.111718422741-0.00235286391840.675221028873-0.04477313458520.02990626113011.126480788150.01231579193640.5198402038625.216308027410.215729647865.6122086178
291.59545751709-0.533441329454-0.631822512752-0.5865512099680.1222795803270.199777718874-0.0746053222399-0.5523642226980.099081096688-0.0714692253585-0.06749931725050.119633764252-0.280968739742-0.3555640951250.0009179471447290.760939069201-0.04769680618860.008974365091290.81978500696-0.04050658161760.50794528242431.183629676914.100697141756.4342905743
300.444720137951-0.157992192951-0.07283510460880.04979038796490.08551873044590.1152065829190.223092062549-0.4964147862880.439418457839-0.06233886557520.285000926050.220329828861-0.7987187761990.1774835702050.0006010525150370.6103444907340.01588039716880.03225441659931.001211459460.03017461519310.54279652861321.93171915514.922956399759.9010290794
310.0195561064629-0.07445428468420.2584573110810.1323475520920.1813753858630.294035214970.138288807691-0.6883749177560.1517322984020.1841105279050.03990357705220.0416481757773-0.280073897333-0.2667458105120.0001419017217990.713360102676-0.111854226562-0.02716377553641.357911520530.1099966235490.59341570824428.710111505512.652461964475.0863651902
320.585513534136-0.1411638460660.1086946553060.7997929147010.1441335839080.04281532480520.0460590218083-0.389967587578-0.415542392807-0.3085499327110.2167559624070.08871156519880.5647184770410.142047227107-4.28334433032E-50.619300686262-0.02407380218730.01495370494420.887873103427-0.01054728732870.59330256262335.28819264137.1232084136650.3316424998
331.547060149810.2593584815420.7548504832210.526260295557-0.3156436824190.669475577593-0.0327203350438-0.5881211545450.3931329166730.002812246046820.101752661684-0.264980635387-0.03753798779560.3545398840621.1819385603E-50.566693423162-0.0406008108802-0.003623157117880.822780848268-0.06879077061040.58799415600346.220810844912.029010072154.3386002479
341.6416159705-1.37647551067-0.3270152312811.63591287509-0.2755515240170.3800598328460.0553320560178-0.436868093395-0.610188051091-0.133319476073-0.0526872512237-0.3740237639460.3577285150290.252557362594-0.0002393130115640.596025462002-0.01196458754270.01329484100320.8407251499120.07213423981260.63914827170442.97741681945.8846227686952.2880987775
350.3526242982740.0572741930140.3361186034480.5367861547410.4942119373570.6315553180460.7041614958470.0300512321766-1.49349509217-0.363727230405-0.2521101158410.1376465809971.270019796470.1915068945230.01218621352631.20482698121-0.0557572731869-6.45773005259E-50.9133724177610.09868103808920.96885248766532.7327589671-6.1111774436852.0016710857
360.0369532824930.08407321687080.0565115584060.07563395090470.1492492812750.1599014078730.1663943113780.442098774714-0.6239893632460.102139202298-0.1173006842060.2272021172930.760395211655-1.0525888025-0.0002203767795491.21132977709-0.171432364870.01477187828020.840520311506-0.03657910015161.1975219831827.8114552885-8.6408082093545.6080778909
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 43 through 54 )AA43 - 541 - 12
22chain 'A' and (resid 55 through 99 )AA55 - 9913 - 57
33chain 'A' and (resid 100 through 137 )AA100 - 13758 - 95
44chain 'A' and (resid 138 through 148 )AA138 - 14896 - 106
55chain 'A' and (resid 149 through 163 )AA149 - 163107 - 121
66chain 'A' and (resid 164 through 195 )AA164 - 195122 - 153
77chain 'A' and (resid 196 through 212 )AA196 - 212154 - 170
88chain 'A' and (resid 213 through 224 )AA213 - 224171 - 182
99chain 'A' and (resid 225 through 250 )AA225 - 250183 - 208
1010chain 'B' and (resid 62 through 83 )BB62 - 831 - 22
1111chain 'B' and (resid 84 through 98 )BB84 - 9823 - 37
1212chain 'B' and (resid 99 through 113 )BB99 - 11338 - 52
1313chain 'C' and (resid 43 through 148 )CC43 - 1481 - 106
1414chain 'C' and (resid 149 through 163 )CC149 - 163107 - 118
1515chain 'C' and (resid 164 through 212 )CC164 - 212119 - 167
1616chain 'C' and (resid 213 through 250 )CC213 - 250168 - 205
1717chain 'D' and (resid 59 through 83 )DD59 - 831 - 25
1818chain 'D' and (resid 84 through 98 )DD84 - 9826 - 40
1919chain 'D' and (resid 99 through 113 )DD99 - 11341 - 55
2020chain 'E' and (resid 43 through 117 )EE43 - 1171 - 75
2121chain 'E' and (resid 118 through 148 )EE118 - 14876 - 106
2222chain 'E' and (resid 149 through 163 )EE149 - 163107 - 118
2323chain 'E' and (resid 164 through 224 )EE164 - 224119 - 179
2424chain 'E' and (resid 225 through 250 )EE225 - 250180 - 205
2525chain 'F' and (resid 59 through 83 )FF59 - 831 - 25
2626chain 'F' and (resid 84 through 98 )FF84 - 9826 - 40
2727chain 'F' and (resid 99 through 112 )FF99 - 11241 - 54
2828chain 'G' and (resid 43 through 88 )GG43 - 881 - 46
2929chain 'G' and (resid 89 through 125 )GG89 - 12547 - 83
3030chain 'G' and (resid 126 through 137 )GG126 - 13784 - 95
3131chain 'G' and (resid 138 through 163 )GG138 - 16396 - 118
3232chain 'G' and (resid 164 through 178 )GG164 - 178119 - 133
3333chain 'G' and (resid 179 through 212 )GG179 - 212134 - 167
3434chain 'G' and (resid 213 through 249 )GG213 - 249168 - 204
3535chain 'H' and (resid 59 through 83 )HH59 - 831 - 25
3636chain 'H' and (resid 84 through 112 )HH84 - 11226 - 54

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