PDB-8tbu: Structure of human erythrocyte pyruvate kinase in complex with an...

ID or keywords:

Entry

Database: PDB / ID: 8tbu

Title

Structure of human erythrocyte pyruvate kinase in complex with an allosteric activator Compound 12

Components

Pyruvate kinase PKLR

Keywords

TRANSFERASE/ACTIVATOR / Erythrocyte pyruvate kinase / PKR / PKLR / Allosteric Activator / Glycolysis / TRANSFERASE / TRANSFERASE-ACTIVATOR complex

Function / homology

Function and homology information

pyruvate kinase complex / pyruvate biosynthetic process / SARS-CoV-1-host interactions / ChREBP activates metabolic gene expression / pyruvate kinase / pyruvate kinase activity / Pyruvate metabolism / monosaccharide binding / response to metal ion / Glycolysis ...
Similarity search - Function

Biological species

Homo sapiens (human)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.35 Å

Authors

Jin, L. / Padyana, A.

Funding support

1items

Citation

Journal: Chemmedchem / Year: 2024
Title: Structure-Based Design of AG-946, a Pyruvate Kinase Activator.
Authors: Liu, T. / Padyana, A.K. / Judd, E.T. / Jin, L. / Hammoudeh, D. / Kung, C. / Dang, L.

History

Deposition	Jun 29, 2023	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Dec 27, 2023	Provider: repository / Type: Initial release
Revision 1.1	Mar 20, 2024	Group: Database references / Category: citation / citation_author Item: _citation.journal_volume / _citation.year / _citation_author.identifier_ORCID

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	8tbu.cif.gz	1.4 MB	Display	PDBx/mmCIF format
PDB format	pdb8tbu.ent.gz	1.2 MB	Display	PDB format
PDBx/mmJSON format	8tbu.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/tb/8tbu ftp://data.pdbj.org/pub/pdb/validation_reports/tb/8tbu	HTTPS FTP

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Related structure data

Related structure data	8tbsC 8tbtC C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#50 - Feb 2004 Glycolytic Enzymes similarity (15) #102 - Jun 2008 Lactate Dehydrogenase similarity (2) #194 - Feb 2016 Designer Insulins similarity (1) #263 - Nov 2021 Acetohydroxyacid Synthase similarity (1) #257 - May 2021 Fetal Hemoglobin similarity (1) #14 - Feb 2001 Insulin similarity (1) #222 - Jun 2018 Proteins and Nanoparticles similarity (1)

Deposited unit

A: Pyruvate kinase PKLR

B: Pyruvate kinase PKLR

C: Pyruvate kinase PKLR

D: Pyruvate kinase PKLR

E: Pyruvate kinase PKLR

F: Pyruvate kinase PKLR

G: Pyruvate kinase PKLR

H: Pyruvate kinase PKLR

hetero molecules

474 kDa, 8 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: Pyruvate kinase PKLR

B: Pyruvate kinase PKLR

C: Pyruvate kinase PKLR

D: Pyruvate kinase PKLR

hetero molecules

defined by author&software
237 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

E: Pyruvate kinase PKLR

F: Pyruvate kinase PKLR

G: Pyruvate kinase PKLR

H: Pyruvate kinase PKLR

hetero molecules

defined by author&software
237 kDa, 4 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	110.323, 122.845, 378.582
Angle α, β, γ (deg.)	90.00, 90.00, 90.00
Int Tables number	19
Space group name H-M	P2₁2₁2₁

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Protein / Sugars , 2 types, 16 molecules ABCDEFGH

#1: Protein

Pyruvate kinase PKLR / Pyruvate kinase 1 / Pyruvate kinase isozymes L/R / R-type/L-type pyruvate kinase / Red cell/liver ...

Mass: 58665.176 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Details: Red blood cell/liver isoform / Source: (gene. exp.)

Homo sapiens (human) / Gene: PKLR, PK1, PKL / Production host:

Escherichia coli (E. coli) / References: UniProt: P30613, pyruvate kinase

#4: Sugar

ChemComp-FBP / 1,6-di-O-phosphono-beta-D-fructofuranose / BETA-FRUCTOSE-1,6-DIPHOSPHATE / FRUCTOSE-1,6-BISPHOSPHATE / 1,6-di-O-phosphono-beta-D-fructose / 1,6-di-O-phosphono-D-fructose / 1,6-di-O-phosphono-fructose

Type: D-saccharide, beta linking / Mass: 340.116 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C₆H₁₄O₁₂P₂

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Non-polymers , 5 types, 1564 molecules

#2: Chemical	ChemComp-I0R / 6-[(4-hydroxyphenyl)methyl]-2,4-dimethyl-4,6-dihydro-5H-[1,3]thiazolo[5',4':4,5]pyrrolo[2,3-d]pyridazin-5-one Mass: 326.373 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C₁₆H₁₄N₄O₂S / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical	ChemComp-PYR / PYRUVIC ACID Mass: 88.062 Da / Num. of mol.: 6 / Source method: obtained synthetically / Formula: C₃H₄O₃
#5: Chemical	ChemComp-MN / MANGANESE (II) ION Mass: 54.938 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Mn
#6: Chemical	ChemComp-K / POTASSIUM ION Mass: 39.098 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: K
#7: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 1544 / Source method: isolated from a natural source / Formula: H₂O

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Details

Has ligand of interest	Y

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.73 Å³/Da / Density % sol: 55 %
Crystal grow	Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 10 mM MnSO4, 50 mM MES/KOH pH 6.5, 10 %(w/v) PEG 8000

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: SSRF / Beamline: BL19U1 / Wavelength: 0.97918 Å
Detector	Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Apr 7, 2017
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 0.97918 Å / Relative weight: 1
Reflection	Resolution: 2.35→49.88 Å / Num. obs: 213360 / % possible obs: 99.57 % / Redundancy: 5.7 % / R_merge(I) obs: 0.08 / Net I/σ(I): 23.8
Reflection shell	Resolution: 2.35→2.434 Å / R_merge(I) obs: 0.535 / Mean I/σ(I) obs: 3.9 / Num. unique obs: 20443

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Processing

Software

Name	Version	Classification
PHENIX	1.17.1_3660	refinement
HKL-2000		data reduction
HKL-2000		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 2.35→49.88 Å / SU ML: 0.27 / Cross valid method: FREE R-VALUE / σ(F): 1.96 / Phase error: 26.8 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.2457	10677	5.01 %
R_work	0.2016	-	-
obs	0.2038	213233	99.59 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Refinement step

Cycle: LAST / Resolution: 2.35→49.88 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	28996	0	298	1544	30838

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.004	29774
X-RAY DIFFRACTION	f_angle_d	0.675	40382
X-RAY DIFFRACTION	f_dihedral_angle_d	22.12	4272
X-RAY DIFFRACTION	f_chiral_restr	0.046	4668
X-RAY DIFFRACTION	f_plane_restr	0.004	5256

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.35-2.38	0.3142	305	0.2637	6001	X-RAY DIFFRACTION	90
2.38-2.4	0.3226	379	0.2636	6760	X-RAY DIFFRACTION	100
2.4-2.43	0.2948	371	0.2475	6627	X-RAY DIFFRACTION	100
2.43-2.46	0.2909	357	0.2441	6763	X-RAY DIFFRACTION	100
2.46-2.5	0.2751	355	0.2341	6677	X-RAY DIFFRACTION	100
2.5-2.53	0.2832	340	0.2278	6691	X-RAY DIFFRACTION	100
2.53-2.57	0.2739	373	0.2266	6765	X-RAY DIFFRACTION	100
2.57-2.61	0.2696	355	0.225	6673	X-RAY DIFFRACTION	100
2.61-2.65	0.268	363	0.2287	6699	X-RAY DIFFRACTION	100
2.65-2.69	0.291	354	0.2297	6777	X-RAY DIFFRACTION	100
2.69-2.74	0.2903	331	0.2243	6715	X-RAY DIFFRACTION	100
2.74-2.79	0.2617	347	0.2199	6731	X-RAY DIFFRACTION	100
2.79-2.84	0.294	351	0.2208	6782	X-RAY DIFFRACTION	100
2.84-2.9	0.2724	335	0.222	6751	X-RAY DIFFRACTION	100
2.9-2.96	0.2778	382	0.2185	6684	X-RAY DIFFRACTION	100
2.96-3.03	0.2517	368	0.2101	6730	X-RAY DIFFRACTION	100
3.03-3.11	0.2528	347	0.207	6766	X-RAY DIFFRACTION	100
3.11-3.19	0.2692	355	0.2164	6715	X-RAY DIFFRACTION	100
3.19-3.28	0.2616	349	0.2119	6806	X-RAY DIFFRACTION	100
3.28-3.39	0.2562	367	0.213	6747	X-RAY DIFFRACTION	100
3.39-3.51	0.257	362	0.2055	6804	X-RAY DIFFRACTION	100
3.51-3.65	0.2501	336	0.1933	6770	X-RAY DIFFRACTION	100
3.65-3.82	0.209	354	0.1826	6813	X-RAY DIFFRACTION	100
3.82-4.02	0.2044	354	0.1786	6811	X-RAY DIFFRACTION	100
4.02-4.27	0.2065	333	0.1699	6840	X-RAY DIFFRACTION	100
4.27-4.6	0.2049	376	0.1574	6839	X-RAY DIFFRACTION	100
4.6-5.06	0.2099	363	0.1709	6844	X-RAY DIFFRACTION	100
5.06-5.79	0.2482	334	0.1978	6936	X-RAY DIFFRACTION	100
5.79-7.29	0.2395	390	0.2121	6939	X-RAY DIFFRACTION	100
7.3-49.88	0.2443	391	0.208	7100	X-RAY DIFFRACTION	98

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	1.6633	-0.2131	0.6878	0.5226	-0.2774	1.3018	-0.006	-0.3189	0.1262	0.0016	-0.0362	-0.1255	-0.125	0.0167	0.0438	0.271	-0.0461	0.0365	0.4549	-0.0558	0.4117	8.5912	-15.7584	-92.4717
2	1.083	-0.8103	0.1853	1.952	-0.1341	0.8353	0.5211	1.0217	-0.5657	-0.8271	-0.4219	-0.3506	0.5116	0.4776	-0.0559	0.9706	0.1776	0.0295	1.0369	-0.0775	0.9812	9.1026	-42.1684	-119.737
3	1.2144	-0.0222	0.2591	0.6018	-0.2571	1.2179	0.0508	-0.1902	-0.1192	-0.0238	-0.0462	-0.0208	0.1152	-0.0282	0.0193	0.2119	-0.0194	0.0076	0.3273	-0.0036	0.3256	0.3378	-24.9091	-96.8068
4	1.4922	-0.3778	-0.6773	1.0099	0.0937	1.0791	-0.0551	-0.1363	0.0523	0.0908	0.1318	-0.0454	-0.1129	0.2414	-0.055	0.2362	0.0011	-0.0157	0.5055	-0.0024	0.292	-3.2256	-13.77	-73.4705
5	0.702	-0.4948	0.722	0.5169	-0.4423	1.5706	0.0412	0.1698	0.0316	-0.0844	-0.0154	-0.0924	-0.0764	0.2749	-0.0397	0.2878	-0.0457	0.0546	0.3802	-0.0423	0.3224	-8.0126	-11.6716	-125.6037
6	1.9868	0.0886	-0.3482	2.0442	0.0664	0.4602	-0.0058	0.4594	-0.206	0.4174	-0.2958	-0.2118	-0.0534	0.2444	0.0749	0.7423	-0.276	-0.0187	0.9009	0.1359	1.2294	17.2308	9.0556	-113.9514
7	1.7323	0.1276	0.9972	0.4077	-0.1107	1.1465	-0.1798	0.2554	0.5678	0.0911	0.0513	-0.1596	-0.5595	0.6984	0.034	0.6161	-0.2205	-0.0329	0.5503	0.0051	0.572	-0.1822	9.4376	-124.2551
8	1.6259	-0.0319	0.63	0.1351	-0.2476	0.8524	-0.0545	-0.0173	0.0808	-0.0302	0.0496	-0.0064	-0.0406	-0.0279	-0.0019	0.2728	-0.0302	0.022	0.248	-0.034	0.2675	-18.4261	-8.1319	-120.3892
9	0.5054	0.5021	0.9023	0.58	0.3707	1.7063	-0.0037	-0.112	-0.0303	0.138	-0.0375	0.0151	0.1199	-0.0613	0.091	0.2287	0.0154	0.0344	0.3196	0.018	0.2835	-45.1117	-13.6	-65.7172
10	1.9025	-0.5936	1.4481	1.044	-0.1386	1.7766	-0.0146	0.1572	0.0578	-0.1621	-0.1441	0.5731	-0.418	-0.2376	0.1541	0.4692	0.156	-0.0407	0.5445	-0.0094	0.5018	-54.1724	5.0494	-67.7475
11	0.9454	0.2279	0.5159	1.8874	-1.278	2.1347	-0.309	-0.314	0.4324	0.1158	0.1867	0.2213	-0.642	-0.3432	0.1161	0.4794	0.084	-0.1013	0.425	-0.0849	0.5793	-46.2572	11.7688	-61.5665
12	1.126	0.0791	0.563	0.8052	0.2114	0.9603	-0.0884	0.0317	0.1426	-0.0164	-0.0261	-0.0119	-0.1625	0.0767	0.0843	0.2585	-0.0243	-0.0121	0.269	0.0323	0.2903	-45.2177	-4.1804	-74.9151
13	1.3763	0.0441	-0.1954	0.672	0.5037	1.0388	-0.0472	-0.0388	-0.0849	0.0801	0.1095	-0.1018	0.1085	0.1661	-0.0689	0.2571	0.0253	-0.0182	0.3163	-0.0065	0.2665	-25.5412	-12.0432	-62.108
14	0.9468	-0.1713	0.945	0.328	0.3392	1.5747	-0.102	0.3126	0.2274	-0.0204	-0.0278	0.1188	-0.1847	0.108	0.097	0.2637	0.0298	0.0181	0.4165	0.0404	0.3882	-62.3851	-10.5885	-96.7928
15	1.1019	0.0938	-0.114	0.8001	-0.0324	1.6819	0.1013	-0.2962	-0.3982	0.5343	-0.0017	0.3394	0.2414	-0.2643	-0.0537	0.4742	-0.0987	0.1068	0.4238	0.0393	0.5979	-66.8297	-36.1841	-75.8076
16	0.984	0.2106	0.3437	0.9124	0.034	1.0198	0.0383	0.1049	-0.1729	0.0267	-0.0652	0.0543	0.1734	0.024	0.0394	0.2123	0.0206	0.0224	0.2882	-0.0111	0.3181	-58.5737	-25.8705	-90.8774
17	-0.0021	0.2209	0.0864	0.857	0.24	0.8906	-0.0882	-0.0293	0.0378	-0.1919	0.0517	-0.1213	-0.1514	0.3959	0.0003	0.2478	-0.0277	0.0196	0.401	0.0543	0.328	-46.8574	-11.2549	-98.1975
18	1.6265	0.1091	-0.776	1.073	0.2216	0.7754	-0.0784	0.0826	0.1037	-0.1023	0.1218	-0.0133	-0.0615	-0.0579	-0.034	0.2616	-0.0212	0.0055	0.347	-0.0165	0.2719	-52.0539	-12.5403	-115.7385
19	0.573	0.2219	-0.3579	2.1938	0.2898	0.7726	-0.0991	-0.3502	-0.1626	0.2768	-0.102	-0.3486	0.7434	0.3974	0.1168	0.6893	0.088	0.0024	0.3803	0.0726	0.3947	20.0237	-76.8137	-194.4221
20	0.8065	0.0413	-0.3086	1.0255	-0.6464	2.151	-0.0544	0.1658	-0.1154	-0.1583	-0.0503	0.1861	1.3423	-0.877	0.088	1.0671	-0.5096	0.0482	0.5495	-0.0951	0.4103	-2.1804	-91.6984	-203.4715
21	0.7399	0.321	-0.1256	1.406	-0.0084	1.4917	-0.0103	0.0042	-0.0769	-0.2825	-0.1153	-0.0671	0.8461	-0.1086	0.0731	0.9659	-0.1881	0.0406	0.3556	0.0149	0.3785	7.3593	-92.8784	-180.7359
22	0.6539	0.5121	0.2684	0.9221	-0.8649	2.3447	0.1372	0.1316	0.1531	-0.0759	0.0356	0.1241	0.2568	-0.6119	-0.136	0.2931	-0.0067	-0.0441	0.3298	-0.0136	0.291	7.2624	-66.2347	-224.0244
23	0.9136	-0.897	-0.165	1.8909	-0.5741	0.4323	0.1402	0.2125	-0.5186	-0.3447	-0.1797	0.405	0.361	0.1991	-0.025	0.6518	0.2778	0.0165	0.5374	-0.0451	0.5819	32.8444	-87.4631	-223.2664
24	0.3685	0.1322	-0.3502	0.8972	0.1322	1.6067	0.0172	0.065	0.0516	-0.048	-0.0502	-0.0261	0.0839	0.001	0.0151	0.2635	0.0495	-0.0152	0.2501	0.0308	0.2713	15.0066	-60.0602	-216.8987
25	0.8631	-0.2727	0.761	2.1213	-0.574	1.4997	-0.2442	-0.0456	0.3962	0.0977	-0.0111	0.0132	-1.0142	-0.3188	0.0876	0.8769	0.1762	-0.0938	0.3154	-0.0623	0.4007	5.7261	-28.5817	-177.9499
26	1.2892	0.0783	-0.1582	1.7273	-1.1611	1.9866	0.0063	-0.2056	0.0471	-0.1983	0.1515	0.6963	-0.8983	-1.3313	-0.0208	0.7994	0.5765	-0.0657	0.9659	-0.0628	0.6071	-15.9126	-31.0948	-178.0069
27	0.9497	-0.0724	0.2058	0.6304	-0.6164	1.4894	-0.0481	-0.0735	0.0292	0.1105	-0.0542	0.056	-0.6447	-0.3424	0.1066	0.5031	0.1123	-0.0347	0.3519	-0.0309	0.338	2.8813	-39.5911	-174.7398
28	0.5043	-0.6718	0.2672	1.3365	0.3372	1.478	-0.0658	0.0229	0.2189	0.2059	-0.0987	-0.0898	-0.835	-0.1247	0.1587	0.7368	0.1288	-0.0328	0.3088	0.0379	0.3936	6.336	-30.0883	-197.8559
29	0.5809	-0.3226	0.1304	0.7089	-0.8374	2.0265	0.0817	-0.2565	-0.1041	0.0454	0.0131	0.052	-0.0469	-0.4659	-0.0705	0.2332	-0.0288	0.0158	0.298	-0.0263	0.256	6.8009	-56.7527	-154.6039
30	0.9947	1.056	0.0368	2.0856	-0.3593	0.6886	0.1334	-0.2805	0.3311	0.2255	-0.2058	0.3063	-0.2897	0.1468	0.0327	0.454	-0.2051	-0.0372	0.4533	-0.0195	0.4506	32.3913	-35.6526	-155.9698
31	0.7154	-0.0219	0.3049	0.7632	-0.2628	1.8559	-0.0216	-0.0161	0.0229	0.1194	-0.0544	-0.1214	-0.2212	0.1368	0.0656	0.2892	-0.0695	-0.0266	0.2494	-0.0025	0.2896	18.5339	-52.3264	-159.8369
32	0.8007	0.0537	0.554	1.6498	0.1127	2.1526	0.1069	-0.1395	-0.1457	-0.0251	-0.0841	0.1203	0.5148	-0.2166	-0.0078	0.3806	-0.0995	-0.0192	0.3074	0.0385	0.327	8.8318	-75.644	-163.7739

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details
1	X-RAY DIFFRACTION	1	chain 'A' and (resid 58 through 174 )
2	X-RAY DIFFRACTION	2	chain 'A' and (resid 175 through 251 )
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 252 through 450 )
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 451 through 574 )
5	X-RAY DIFFRACTION	5	chain 'B' and (resid 57 through 183 )
6	X-RAY DIFFRACTION	6	chain 'B' and (resid 184 through 244 )
7	X-RAY DIFFRACTION	7	chain 'B' and (resid 245 through 290 )
8	X-RAY DIFFRACTION	8	chain 'B' and (resid 291 through 574 )
9	X-RAY DIFFRACTION	9	chain 'C' and (resid 50 through 141 )
10	X-RAY DIFFRACTION	10	chain 'C' and (resid 142 through 265 )
11	X-RAY DIFFRACTION	11	chain 'C' and (resid 266 through 301 )
12	X-RAY DIFFRACTION	12	chain 'C' and (resid 302 through 450 )
13	X-RAY DIFFRACTION	13	chain 'C' and (resid 451 through 574 )
14	X-RAY DIFFRACTION	14	chain 'D' and (resid 57 through 141 )
15	X-RAY DIFFRACTION	15	chain 'D' and (resid 142 through 251 )
16	X-RAY DIFFRACTION	16	chain 'D' and (resid 252 through 413 )
17	X-RAY DIFFRACTION	17	chain 'D' and (resid 414 through 450 )
18	X-RAY DIFFRACTION	18	chain 'D' and (resid 451 through 574 )
19	X-RAY DIFFRACTION	19	chain 'E' and (resid 51 through 88 )
20	X-RAY DIFFRACTION	20	chain 'E' and (resid 89 through 450 )
21	X-RAY DIFFRACTION	21	chain 'E' and (resid 451 through 574 )
22	X-RAY DIFFRACTION	22	chain 'F' and (resid 57 through 141 )
23	X-RAY DIFFRACTION	23	chain 'F' and (resid 142 through 251 )
24	X-RAY DIFFRACTION	24	chain 'F' and (resid 252 through 574 )
25	X-RAY DIFFRACTION	25	chain 'G' and (resid 50 through 265 )
26	X-RAY DIFFRACTION	26	chain 'G' and (resid 266 through 331 )
27	X-RAY DIFFRACTION	27	chain 'G' and (resid 332 through 450 )
28	X-RAY DIFFRACTION	28	chain 'G' and (resid 451 through 574 )
29	X-RAY DIFFRACTION	29	chain 'H' and (resid 57 through 141 )
30	X-RAY DIFFRACTION	30	chain 'H' and (resid 142 through 265 )
31	X-RAY DIFFRACTION	31	chain 'H' and (resid 266 through 430 )
32	X-RAY DIFFRACTION	32	chain 'H' and (resid 431 through 574 )

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