根拠: homology, Protein is a tetramer in solution as shown extensively by the literature. Often the P21212 space groups have a symmetry axis that goes down the strong dimer interface as as the case ...根拠: homology, Protein is a tetramer in solution as shown extensively by the literature. Often the P21212 space groups have a symmetry axis that goes down the strong dimer interface as as the case here. As such the Asymmetric unit here is what the literature calls the tight dimer.
温度: 277 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 6.5 詳細: In drop 1:1 mixture of sample buffer and crystal condition Buffer :10mg/ml protein, 10 mM phosphate potassium, 100 mM KCl at pH 7.0 Crystal Condition: 0.1 M sodium cacodylate (pH 6.5), 0.2 M ...詳細: In drop 1:1 mixture of sample buffer and crystal condition Buffer :10mg/ml protein, 10 mM phosphate potassium, 100 mM KCl at pH 7.0 Crystal Condition: 0.1 M sodium cacodylate (pH 6.5), 0.2 M calcium acetate and 40 % (w/v) PEG 300 at 277 K
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データ収集
回折
平均測定温度: 100 K / Serial crystal experiment: N
放射光源
由来: シンクロトロン / サイト: ALS / ビームライン: 5.0.3 / 波長: 0.9765 Å
検出器
タイプ: ADSC QUANTUM 315 / 検出器: CCD / 日付: 2019年9月7日
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.9765 Å / 相対比: 1
反射
解像度: 1.63→39.02 Å / Num. obs: 30727 / % possible obs: 98.8 % / 冗長度: 7.9 % / Biso Wilson estimate: 18.07 Å2 / CC1/2: 0.871 / Rpim(I) all: 0.04 / Rrim(I) all: 0.115 / Net I/σ(I): 22.2