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- PDB-8s5c: Cryo-EM structure of Arf1-decorated membrane tubules -

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Basic information

Entry
Database: PDB / ID: 8s5c
TitleCryo-EM structure of Arf1-decorated membrane tubules
ComponentsADP-ribosylation factor 1
KeywordsTRANSPORT PROTEIN / Arf1 / protein-membrane interaction / membrane tubules / helical structure
Function / homology
Function and homology information


Synthesis of PIPs at the plasma membrane / VxPx cargo-targeting to cilium / Synthesis of PIPs at the Golgi membrane / Intra-Golgi traffic / trans-Golgi Network Vesicle Budding / protein localization to Golgi membrane / regulation of Golgi organization / organelle membrane contact site / COPI-dependent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport ...Synthesis of PIPs at the plasma membrane / VxPx cargo-targeting to cilium / Synthesis of PIPs at the Golgi membrane / Intra-Golgi traffic / trans-Golgi Network Vesicle Budding / protein localization to Golgi membrane / regulation of Golgi organization / organelle membrane contact site / COPI-dependent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / Golgi vesicle transport / positive regulation of mitochondrial fusion / regulation of fatty acid metabolic process / Golgi to plasma membrane transport / positive regulation of mitochondrial fission / endoplasmic reticulum to Golgi vesicle-mediated transport / vesicle-mediated transport / small monomeric GTPase / intracellular protein transport / macroautophagy / GTPase activity / GTP binding / Golgi apparatus / plasma membrane / cytosol / cytoplasm
Similarity search - Function
ADP-ribosylation factor 1-5 / Small GTPase superfamily, ARF type / small GTPase Arf family profile. / Sar1p-like members of the Ras-family of small GTPases / Small GTPase superfamily, ARF/SAR type / ADP-ribosylation factor family / ARF-like small GTPases; ARF, ADP-ribosylation factor / Rab subfamily of small GTPases / Small GTP-binding protein domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE / ADP-ribosylation factor 1
Similarity search - Component
Biological speciesSaccharomyces cerevisiae (brewer's yeast)
MethodELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsHaupt, C. / Semchonok, D.A. / Stubbs, M.T. / Bacia, K. / Desfosses, A. / Kastritis, P.L. / Hamdi, F.
Funding support Germany, 2items
OrganizationGrant numberCountry
German Federal Ministry for Education and Research03Z22HN23 Germany
German Federal Ministry for Education and Research03Z22HI2 Germany
CitationJournal: To Be Published
Title: Cryo-EM structure of Arf1-decorated membrane tubules
Authors: Haupt, C. / Semchonok, D.A. / Stubbs, M.T. / Bacia, K. / Desfosses, A.
History
DepositionFeb 23, 2024Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jan 22, 2025Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: ADP-ribosylation factor 1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)21,1163
Polymers20,5521
Non-polymers5642
Water362
1
A: ADP-ribosylation factor 1
hetero molecules
x 205


Theoretical massNumber of molelcules
Total (without water)4,328,778615
Polymers4,213,250205
Non-polymers115,528410
Water3,693205
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
point symmetry operation204
MethodUCSF CHIMERA

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Components

#1: Protein ADP-ribosylation factor 1


Mass: 20552.438 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: A myristoyl group (derived from myristic acid) is covalently attached to the N-terminus via an amide bond.
Source: (gene. exp.) Saccharomyces cerevisiae (brewer's yeast)
Gene: ARF1, YDL192W, D1244 / Production host: Escherichia coli (E. coli) / References: UniProt: P11076, small monomeric GTPase
#2: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Mg / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical ChemComp-GSP / 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE


Mass: 539.246 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H16N5O13P3S / Feature type: SUBJECT OF INVESTIGATION
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY
Has protein modificationN

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: HELICAL ARRAY / 3D reconstruction method: helical reconstruction

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Sample preparation

ComponentName: Arf1 coated membrane tubules / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT
Molecular weightExperimental value: NO
Source (natural)Organism: Saccharomyces cerevisiae (brewer's yeast)
Source (recombinant)Organism: Escherichia coli (E. coli)
Buffer solutionpH: 6.8
Buffer component
IDConc.NameFormulaBuffer-ID
120 mMHEPESHEPES1
250 mMpotassium acetateKAc1
31.2 mMmagnesium chlorideMgCl21
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid mesh size: 200 divisions/in. / Grid type: Quantifoil R2/1
VitrificationInstrument: LEICA EM GP / Cryogen name: ETHANE / Humidity: 80 % / Chamber temperature: 277 K

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Electron microscopy imaging

MicroscopyModel: TFS GLACIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: OTHER
Electron lensMode: BRIGHT FIELD / Nominal magnification: 92000 X / Nominal defocus max: 2800 nm / Nominal defocus min: 600 nm / Cs: 2.7 mm / C2 aperture diameter: 70 µm / Alignment procedure: COMA FREE
Specimen holderCryogen: NITROGEN / Specimen holder model: OTHER
Image recordingElectron dose: 21 e/Å2 / Film or detector model: FEI FALCON IV (4k x 4k) / Num. of grids imaged: 1 / Num. of real images: 12483
Image scansWidth: 4096 / Height: 4096

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Processing

EM software
IDNameVersionCategory
1cryoSPARCv4.3.1, v4.4.2particle selection
2EPU2.2.0.65RELimage acquisition
4cryoSPARCv4.3.1, v4.4.2CTF correction
7ISOLDEmodel fitting
11cryoSPARCv4.3.1, v4.4.2classification
12cryoSPARCv4.3.1, v4.4.23D reconstruction
13PHENIX1.21-5187model refinement
14Coot0.9.7 ELmodel refinement
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Helical symmertyAngular rotation/subunit: 23.27 ° / Axial rise/subunit: 4.328 Å / Axial symmetry: C2
Particle selectionNum. of particles selected: 1489051
3D reconstructionResolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 127727 / Algorithm: FOURIER SPACE / Num. of class averages: 1 / Symmetry type: HELICAL
Atomic model buildingProtocol: OTHER / Space: REAL
Atomic model buildingPDB-ID: 2ksq

2ksq


Pdb chain-ID: A / Accession code: 2ksq / Chain residue range: 16-178 / Pdb chain residue range: 16-178 / Source name: PDB / Type: experimental model

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