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- PDB-8riv: T2R-TTL-1-K08 complex -

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Basic information

Entry
Database: PDB / ID: 8riv
TitleT2R-TTL-1-K08 complex
Components
  • Stathmin-4
  • Tubulin alpha-1B chain
  • Tubulin beta-2B chain
  • Tubulin tyrosine ligase
KeywordsCELL CYCLE / TUBULIN FOLD / CYTOSKELETON / MICROTUBULE
Function / homology
Function and homology information


tubulin-tyrosine ligase / tubulin-tyrosine ligase activity / positive regulation of axon guidance / microtubule depolymerization / microtubule-based process / regulation of microtubule polymerization or depolymerization / tubulin binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / protein modification process / spindle microtubule ...tubulin-tyrosine ligase / tubulin-tyrosine ligase activity / positive regulation of axon guidance / microtubule depolymerization / microtubule-based process / regulation of microtubule polymerization or depolymerization / tubulin binding / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / protein modification process / spindle microtubule / structural constituent of cytoskeleton / microtubule cytoskeleton organization / microtubule cytoskeleton / neuron projection development / nervous system development / mitotic cell cycle / growth cone / microtubule / neuron projection / protein heterodimerization activity / GTPase activity / GTP binding / Golgi apparatus / ATP binding / metal ion binding / cytoplasm / cytosol
Similarity search - Function
Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. / Stathmin-like (SLD) domain profile. ...Tubulin-tyrosine ligase/Tubulin polyglutamylase / Tubulin-tyrosine ligase family / TTL domain profile. / Stathmin family / Stathmin, conserved site / Stathmin superfamily / Stathmin family / Stathmin family signature 1. / Stathmin family signature 2. / Stathmin-like (SLD) domain profile. / Alpha tubulin / Tubulin-beta mRNA autoregulation signal. / Beta tubulin, autoregulation binding site / Beta tubulin / Tubulin / Tubulin, C-terminal / Tubulin C-terminal domain / Tubulin, conserved site / Tubulin subunits alpha, beta, and gamma signature. / Tubulin/FtsZ family, C-terminal domain / Tubulin/FtsZ-like, C-terminal domain / Tubulin/FtsZ, C-terminal / Tubulin/FtsZ, 2-layer sandwich domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ family, GTPase domain / Tubulin/FtsZ, GTPase domain / Tubulin/FtsZ, GTPase domain superfamily
Similarity search - Domain/homology
: / PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / GUANOSINE-5'-DIPHOSPHATE / GUANOSINE-5'-TRIPHOSPHATE / IMIDAZOLE / Tubulin--tyrosine ligase / Stathmin-4 / Tubulin alpha-1B chain / Tubulin beta-2B chain
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat)
Gallus gallus (chicken)
Bos taurus (cattle)
MethodX-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 2.78 Å
AuthorsBoiarska, Z. / Homer, J.A. / Steinmetz, M.O. / Moses, J.E. / Prota, A.E.P.
Funding supportEuropean Union, Switzerland, United States, Australia, 4items
OrganizationGrant numberCountry
European Union (EU)860070 TubInTrainEuropean Union
Swiss National Science Foundation310030_192566 Switzerland
National Institutes of Health/National Cancer Institute (NIH/NCI)5P30CA045508 United States
Australian Research Council (ARC)FT170100156 Australia
Citation
Journal: Chem Sci / Year: 2024
Title: Modular synthesis of functional libraries by accelerated SuFEx click chemistry.
Authors: Homer, J.A. / Koelln, R.A. / Barrow, A.S. / Gialelis, T.L. / Boiarska, Z. / Steinohrt, N.S. / Lee, E.F. / Yang, W.H. / Johnson, R.M. / Chung, T. / Habowski, A.N. / Vishwakarma, D.S. / ...Authors: Homer, J.A. / Koelln, R.A. / Barrow, A.S. / Gialelis, T.L. / Boiarska, Z. / Steinohrt, N.S. / Lee, E.F. / Yang, W.H. / Johnson, R.M. / Chung, T. / Habowski, A.N. / Vishwakarma, D.S. / Bhunia, D. / Avanzi, C. / Moorhouse, A.D. / Jackson, M. / Tuveson, D.A. / Lyons, S.K. / Lukey, M.J. / Fairlie, W.D. / Haider, S.M. / Steinmetz, M.O. / Prota, A.E. / Moses, J.E.
#1: Journal: Acta Crystallogr D Struct Biol / Year: 2019
Title: Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix.
Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty ...Authors: Dorothee Liebschner / Pavel V Afonine / Matthew L Baker / Gábor Bunkóczi / Vincent B Chen / Tristan I Croll / Bradley Hintze / Li Wei Hung / Swati Jain / Airlie J McCoy / Nigel W Moriarty / Robert D Oeffner / Billy K Poon / Michael G Prisant / Randy J Read / Jane S Richardson / David C Richardson / Massimo D Sammito / Oleg V Sobolev / Duncan H Stockwell / Thomas C Terwilliger / Alexandre G Urzhumtsev / Lizbeth L Videau / Christopher J Williams / Paul D Adams /
Abstract: Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological ...Diffraction (X-ray, neutron and electron) and electron cryo-microscopy are powerful methods to determine three-dimensional macromolecular structures, which are required to understand biological processes and to develop new therapeutics against diseases. The overall structure-solution workflow is similar for these techniques, but nuances exist because the properties of the reduced experimental data are different. Software tools for structure determination should therefore be tailored for each method. Phenix is a comprehensive software package for macromolecular structure determination that handles data from any of these techniques. Tasks performed with Phenix include data-quality assessment, map improvement, model building, the validation/rebuilding/refinement cycle and deposition. Each tool caters to the type of experimental data. The design of Phenix emphasizes the automation of procedures, where possible, to minimize repetitive and time-consuming manual tasks, while default parameters are chosen to encourage best practice. A graphical user interface provides access to many command-line features of Phenix and streamlines the transition between programs, project tracking and re-running of previous tasks.
History
DepositionDec 19, 2023Deposition site: PDBE / Processing site: PDBE
Revision 1.0Mar 27, 2024Provider: repository / Type: Initial release

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Tubulin alpha-1B chain
B: Tubulin beta-2B chain
C: Tubulin alpha-1B chain
D: Tubulin beta-2B chain
E: Stathmin-4
F: Tubulin tyrosine ligase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)265,43524
Polymers261,6316
Non-polymers3,80418
Water2,072115
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: equilibrium centrifugation
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)104.933, 156.362, 183.083
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2

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Components

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Protein , 4 types, 6 molecules ACBDEF

#1: Protein Tubulin alpha-1B chain / Alpha-tubulin ubiquitous / Tubulin K-alpha-1 / Tubulin alpha-ubiquitous chain


Mass: 50204.445 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P81947
#2: Protein Tubulin beta-2B chain


Mass: 49999.887 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: Q6B856
#3: Protein Stathmin-4 / Stathmin-like protein B3 / RB3


Mass: 16844.162 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Stmn4 / Production host: Escherichia coli (E. coli) / References: UniProt: P63043
#4: Protein Tubulin tyrosine ligase


Mass: 44378.496 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Gallus gallus (chicken) / Gene: TTL / Production host: Escherichia coli (E. coli) / References: UniProt: A0A8V0Z8P0

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Non-polymers , 9 types, 133 molecules

#5: Chemical ChemComp-GTP / GUANOSINE-5'-TRIPHOSPHATE


Mass: 523.180 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H16N5O14P3 / Comment: GTP, energy-carrying molecule*YM
#6: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 5 / Source method: obtained synthetically / Formula: Mg
#7: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: Ca
#8: Chemical ChemComp-GDP / GUANOSINE-5'-DIPHOSPHATE


Type: RNA linking / Mass: 443.201 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H15N5O11P2 / Comment: GDP, energy-carrying molecule*YM
#9: Chemical ChemComp-MES / 2-(N-MORPHOLINO)-ETHANESULFONIC ACID


Mass: 195.237 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C6H13NO4S / Comment: pH buffer*YM
#10: Chemical ChemComp-A1H01 / (4-fluoranyl-2-methyl-1~{H}-indol-5-yl) 3,4,5-trimethoxybenzenesulfonate


Mass: 395.402 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C18H18FNO6S / Feature type: SUBJECT OF INVESTIGATION
#11: Chemical ChemComp-IMD / IMIDAZOLE


Mass: 69.085 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C3H5N2
#12: Chemical ChemComp-ACP / PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / ADENOSINE-5'-[BETA, GAMMA-METHYLENE]TRIPHOSPHATE


Mass: 505.208 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C11H18N5O12P3 / Comment: AMP-PCP, energy-carrying molecule analogue*YM
#13: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 115 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.87 Å3/Da / Density % sol: 57.15 %
Crystal growTemperature: 293 K / Method: vapor diffusion / pH: 6.5
Details: 4% PEG 4K, 9-10% glycerol, 30 mM MgCl2, 30 mM CaCl2, 5 mM tyrosine and 100 mM MES/Imidazole pH 6.5

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: SLS / Beamline: X06SA / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Aug 22, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.78→48.11 Å / Num. obs: 144994 / % possible obs: 99.24 % / Redundancy: 7.2 % / Biso Wilson estimate: 84.27 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.09764 / Rpim(I) all: 0.03877 / Rrim(I) all: 0.1052 / Net I/σ(I): 11.64
Reflection shellResolution: 2.78→2.81 Å / Rmerge(I) obs: 1.96 / Mean I/σ(I) obs: 0.79 / Num. unique obs: 4671 / CC1/2: 0.378 / Rpim(I) all: 0.7846 / Rrim(I) all: 2.114

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Processing

Software
NameVersionClassification
PHENIX1.21rc1_5058refinement
XDSdata reduction
XDSdata scaling
PHENIXphasing
RefinementMethod to determine structure: FOURIER SYNTHESIS / Resolution: 2.78→48.11 Å / SU ML: 0.4219 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 25.608
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2307 7233 4.99 %
Rwork0.1799 137722 -
obs0.1825 144955 99.32 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 109.14 Å2
Refinement stepCycle: LAST / Resolution: 2.78→48.11 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms17316 0 235 115 17666
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.002717956
X-RAY DIFFRACTIONf_angle_d0.534124346
X-RAY DIFFRACTIONf_chiral_restr0.03962644
X-RAY DIFFRACTIONf_plane_restr0.00393149
X-RAY DIFFRACTIONf_dihedral_angle_d14.36746615
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.78-2.810.44142350.43044434X-RAY DIFFRACTION94.9
2.81-2.850.38062440.35954618X-RAY DIFFRACTION99.98
2.85-2.880.36562430.31694606X-RAY DIFFRACTION99.88
2.88-2.920.32522440.29814623X-RAY DIFFRACTION99.9
2.92-2.960.3052440.28524567X-RAY DIFFRACTION99.96
2.96-30.31922460.26334660X-RAY DIFFRACTION99.96
3-3.040.31062410.25374598X-RAY DIFFRACTION99.83
3.04-3.090.30442460.2484629X-RAY DIFFRACTION99.65
3.09-3.130.30782420.254578X-RAY DIFFRACTION99.73
3.13-3.190.27212430.25974611X-RAY DIFFRACTION99.86
3.19-3.240.30692430.26324610X-RAY DIFFRACTION99.53
3.24-3.30.32212370.24054595X-RAY DIFFRACTION99.57
3.3-3.360.26132430.21514618X-RAY DIFFRACTION99.33
3.36-3.430.24712340.20324562X-RAY DIFFRACTION98.95
3.43-3.510.26582450.18284567X-RAY DIFFRACTION98.83
3.51-3.590.20232370.17724533X-RAY DIFFRACTION98.41
3.59-3.680.19572340.17374480X-RAY DIFFRACTION97.24
3.68-3.780.21292400.18284600X-RAY DIFFRACTION99.06
3.78-3.890.26822440.16844631X-RAY DIFFRACTION100
3.89-4.010.25272420.17054595X-RAY DIFFRACTION99.98
4.01-4.160.212420.15684636X-RAY DIFFRACTION99.92
4.16-4.320.19712380.14954603X-RAY DIFFRACTION99.88
4.32-4.520.19472410.13254606X-RAY DIFFRACTION99.88
4.52-4.760.1762430.12874641X-RAY DIFFRACTION99.94
4.76-5.060.19182440.14284599X-RAY DIFFRACTION99.94
5.06-5.450.21262490.16124604X-RAY DIFFRACTION99.86
5.45-5.990.21152440.18074612X-RAY DIFFRACTION99.69
5.99-6.860.2842380.19034552X-RAY DIFFRACTION98.22
6.86-8.630.21372380.16724545X-RAY DIFFRACTION98.54
8.63-48.110.19562290.15714609X-RAY DIFFRACTION99.4
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.451324971480.28320029228-0.2555733780745.962864852740.6012553978943.244381974850.008267305282660.4404661155770.300938284397-1.239155015190.558372837475-0.484685743876-1.230011605510.458869826724-0.6739649023320.9415867238-0.1803897721580.240931529060.969981397992-0.2318961712610.70548344012928.792641042394.225663645950.70182982
23.021025992081.35631455681-0.07481782464577.270436868661.862810786942.92561277956-0.01026195390060.391930217149-0.0758587351703-0.1323521960110.617974513807-1.10963900542-0.1360566114311.23501881594-0.542099354360.615356808198-0.07461298000060.07633394362541.14684743255-0.4424550178330.94602131170935.216909944678.21481105955.0201520106
33.54357670978-1.816348545210.5417973855315.826134683930.5319061310595.8502480123-0.0539883126478-0.08269677467220.1999874487510.08088281103480.1555389180130.0652203410976-0.508885155512-0.00321966998047-0.1162626364380.5657109771520.04557524090010.1229423977480.703396216305-0.1412971006920.65221948331519.844045383885.553060831663.5452502187
42.3208824862.25490576095-0.2491081062083.911443843570.1121639065787.204966598490.342446682921-0.6723617317540.6596201754690.4063329226920.1954438567670.354975413632-0.224047273006-0.456937594719-0.4736689506270.648198191830.03970940396920.08124844943550.579335342371-0.1977358260230.60763605960814.593042131783.834992000871.3629794397
52.22714045294-0.670524203423-0.04379679661393.325320935190.6490476673243.52265982896-0.218370654647-0.1575772892390.1364377131421.734644391240.05054793425040.3214701521060.3303091979850.0609961314410.06217169514071.07544153130.004188076801350.2282733941030.708747868321-0.2689658627680.58846707029117.436561451986.649295605881.2536183518
61.4894757845-0.642159527446-0.5612829448474.703485089742.202620833044.083496138690.02993046018420.00730091660292-0.1236773313850.8848231952160.435672703042-0.2260456018270.5474738285980.722673666039-0.4917668512650.6311575197040.0892050272562-0.05450073931330.771817737628-0.222552410690.69088968321125.03853812972.935336058567.1605937063
72.57686953897-2.04246548317-1.378747503286.765940832733.66844949587.361725116420.242190280670.246874205090.600085004251-0.364153422279-0.1208440891130.356226682506-0.431522360448-0.707694911884-0.1835343384540.5036227959590.04709635330150.07999331059720.6363993009650.05471157866230.77006425060215.333778057564.480258750321.2478245024
84.66564397787-2.43734113673-0.6390834179439.265758385471.035767511453.501729388430.1013187884190.4669070236730.606509523773-0.773832626809-0.102893713104-0.024350769936-0.743101570601-0.0233449574635-0.02008214905420.664448811998-0.01531483063850.05062365768850.6403099564030.03217427725870.67315654091817.229964970969.455113250515.9192800843
93.54939307897-0.791431217213-0.255067890113.56188814851.148948518134.716118213150.03945421985940.04145452883150.317136641012-0.1602504217920.0836698538494-0.334913169144-0.3508350010680.322415192749-0.1451297743260.4295546498290.04268017978220.0541626531580.5845780915-0.1344263929870.58897770535623.268675122253.745919360924.1571583689
103.45149271597-0.07448884155720.9611276752334.532589019581.738929092425.65735822388-0.0829064820592-0.03712422876570.461668937257-0.272221245149-0.115661050060.609490768347-0.448907171315-0.5649514084690.2202676903020.489442999860.09543218418940.05620744343860.733674886032-0.1582204742850.63809009486610.567494697162.221783961531.1662422602
116.28080299587-0.4815024674292.843900214442.71003960579-0.6317292945245.00475396150.572610964692-0.61360980390.0152686440378-0.257776849122-0.2224877479330.5091818628570.243139786631-0.769686275264-0.3491710411440.577080539224-0.03979074161080.03627098665580.72148323583-0.2289409662440.6670099462155.3973716706859.119770245837.8643135523
123.51800204404-2.700194968651.755014463535.33766659295-0.7266391325984.65262558843-0.273772732906-0.4760247373040.3732912620220.4890201375580.2012699939790.40551377849-0.264013887459-0.718904086310.009881811546110.5827221590950.06827837795130.09309317550710.908801301328-0.2174096986470.6945801167996.1275434711961.233422409244.2452165337
133.07260431-5.01926946329-3.350625752768.146960305615.737572983584.80533524837-0.272792714996-0.0946640252417-0.4051496022450.332275748771-0.1623141275781.035563915480.408170223255-0.09442200965120.4568021270980.607321090303-0.05860931538560.05997111750210.811610961959-0.109687932040.66263634562315.03966857842.46506603433.8516397798
141.11670877969-0.32635438015-1.225469337294.687595833114.10996501174.23210244107-0.136347065205-0.384320529239-0.01622877881410.9671649206310.531352080306-0.5532526802471.540556980111.08533367152-0.4201675459090.5734994333410.07020955658-0.1001830443420.762148309355-0.02534500015650.68781191287525.339647745238.115167309130.3145305131
155.36415303878-2.456931271320.03410174809487.734728948380.567677955152.304882145020.06581944313180.7368635361090.304171027001-0.726543558983-0.0904985441855-0.0973907956908-0.3735574100090.07899732776160.002749637047980.561213840798-0.1316861126220.03442344551820.6903471434450.03181603046420.47084490333918.124589106836.6380788307-15.6201966739
162.29639862744-0.780956630845-0.02652917151292.83100281214-0.5351558633282.64940141081-0.01095183311490.109772823488-0.122866807054-0.07257551233870.02783055033760.328107199935-0.0393420257346-0.249463808817-0.01047281846360.408707754631-0.03640055269380.03960255328210.529148914984-0.0787892228160.5098263861514.189967329227.1944022491-3.96868727583
172.23913034524-1.01121809938-0.2388943573962.76090285390.7792507322482.87436646063-0.176204636172-0.120523807519-0.0917537599410.1304042285170.04076027392390.5334699829520.0999258426744-0.6255386489370.1192233602130.43668747066-0.1432424147610.0475259014810.611542977834-0.08697008282640.6340203952362.9251235168127.86369797416.29387925535
185.23921581008-3.55492698257-4.93268563564.646042846454.960875746135.752199116-0.502729109479-0.680494972461-0.4835470073590.6686850702150.485006056085-0.2792016210371.163222943341.87826897236-0.03262286560930.560514856010.0532288453246-0.03797863123630.511236794552-0.08281682847730.66719494190624.952297320611.41450952352.85824162588
195.55778897373-3.414737577040.1906973358365.34811070528-0.06783985704273.5849290409-0.02274981689951.25008037529-0.0289773600735-1.09889360095-0.09165154334591.05794593240.149173208117-0.4921784788180.07192030213711.02725620961-0.264830487986-0.1787939479911.292740657490.02942229829960.65959190080115.086565239310.1198827176-41.4670630076
202.889867436030.409165037234-0.3519844879142.61630946414-0.1828671341933.52427157109-0.3824537652621.07423311763-0.543952114467-0.9674503705110.333300993298-0.1383346501230.4849948437820.237126882244-0.01127390231551.16996824343-0.1767685065240.0432723696821.10540446814-0.4452955410620.82269433501523.9982725885-4.61599461579-37.8125753276
212.448425354820.364443736662-0.2153644077122.454062545580.2960447061334.29445418456-0.2314159277460.344200050687-0.173072651995-0.0481263808780.2787687671260.5632235392970.0651341916806-0.643734113042-0.0790255677190.819307986527-0.14197587443-0.02345809765680.935172611065-0.2024665873660.9055896089487.167641413380.807044498521-21.1418075328
223.73039327056-1.71581184332-1.238205137944.078883542051.455973651753.31489861726-0.4614160314220.343502459412-0.659615136040.1643183663190.264258101445-0.1266137467671.211525905810.2951859430130.243891819051.25029184509-0.05017554994510.1275744864520.794606575231-0.3697676124160.97212678599226.0983731021-17.0112829242-23.0143297819
234.84479557939-3.19552747491-1.596194483096.148805447373.193045332642.52656926773-0.574300736569-0.476787966620.5796077544531.193445570450.636879024105-0.1815958240430.03855785637720.493533060049-0.09251361637861.17927977184-0.126712558849-0.1213893490361.19772215509-0.2126602002330.85276053518528.521605727492.312562790882.5199034291
240.573139624656-0.25038760486-0.6845888743553.16228824032.941072335973.23762885423-0.190299414293-0.1509143112050.08818768501970.5722325601090.488853533733-0.3670545356720.6626004359430.732323098789-0.3717141995120.8293573701550.1186785472290.01185290176551.12658498019-0.3220157903541.0669795109842.639496626627.80604964954.66716401822
255.58483241711.08314344193-1.312015538694.84664401081-0.3648353097458.445516006020.04716774291570.515158712715-0.9771257127340.2556566178830.06729003385330.06433191493262.62014299797-1.1223670032-0.188785988721.48307130007-0.2110647980330.1195441813891.02095236978-0.2097271175730.9091217628196.1859030180954.56993437269.3392925401
262.901218993361.428146341710.8048747150541.89116094647-1.903464997674.67842212024-0.152241793485-0.825620432954-0.5560629677120.4622111515570.0389128874038-0.452071387336-0.4054964206061.191639842650.1401518100311.565414104370.286998913840.01362844783131.937717245060.1420981730071.3233835921717.811916973558.0656440612104.030273865
273.832708752290.521860916436-0.4244377970042.95429310494-2.227010112463.13492837197-0.425238077762-0.304553599797-1.44315544404-0.0189424658690.133940492082-0.09565432854691.66172671989-0.2256471973670.306936526241.840297256460.2240639940850.3798437646691.064313478430.1745889179831.43334254645-1.3603978978145.793143048199.565026482
283.768351111840.989202068817-3.960096486720.931905241898-2.126763961457.2875415706-0.4223349678120.0250913040375-0.5198773059130.2682010265690.3186028861250.3158462695881.15166789707-0.3670363382960.118860524661.38307103087-0.06436384155580.1722022759750.920638600118-0.01610938670611.00651035805-1.9328834568759.163053401188.8438920771
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 2 through 102 )AA2 - 1021 - 101
22chain 'A' and (resid 103 through 199 )AA103 - 199102 - 198
33chain 'A' and (resid 200 through 259 )AA200 - 259199 - 258
44chain 'A' and (resid 260 through 306 )AA260 - 306259 - 305
55chain 'A' and (resid 307 through 338 )AA307 - 338306 - 337
66chain 'A' and (resid 339 through 438 )AA339 - 438338 - 437
77chain 'B' and (resid 2 through 27 )BF2 - 271 - 26
88chain 'B' and (resid 28 through 102 )BF28 - 10227 - 99
99chain 'B' and (resid 103 through 214 )BF103 - 214100 - 211
1010chain 'B' and (resid 215 through 259 )BF215 - 259212 - 256
1111chain 'B' and (resid 260 through 296 )BF260 - 296257 - 289
1212chain 'B' and (resid 297 through 373 )BF297 - 373290 - 358
1313chain 'B' and (resid 374 through 401 )BF374 - 401359 - 386
1414chain 'B' and (resid 402 through 437 )BF402 - 437387 - 422
1515chain 'C' and (resid 1 through 128 )CM1 - 1281 - 128
1616chain 'C' and (resid 129 through 259 )CM129 - 259129 - 259
1717chain 'C' and (resid 260 through 401 )CM260 - 401260 - 401
1818chain 'C' and (resid 402 through 440 )CM402 - 440402 - 440
1919chain 'D' and (resid 1 through 60 )DQ1 - 601 - 58
2020chain 'D' and (resid 61 through 237 )DQ61 - 23759 - 235
2121chain 'D' and (resid 238 through 373 )DQ238 - 373236 - 355
2222chain 'D' and (resid 374 through 441 )DQ374 - 441356 - 423
2323chain 'E' and (resid 6 through 46 )EU6 - 461 - 26
2424chain 'E' and (resid 47 through 142 )EU47 - 14227 - 122
2525chain 'F' and (resid 1 through 66 )FV1 - 661 - 66
2626chain 'F' and (resid 67 through 184 )FV67 - 18467 - 160
2727chain 'F' and (resid 185 through 274 )FV185 - 274161 - 247
2828chain 'F' and (resid 275 through 381 )FV275 - 381248 - 343

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