PDB-8qt5: Crystal structure of Arabidopsis thaliana 14-3-3 isoform lambda i...

ID or keywords:

Entry

Database: PDB / ID: 8qt5

Title

Crystal structure of Arabidopsis thaliana 14-3-3 isoform lambda in complex with a phosphopeptide from the transcription factor BZR1.

Components

14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

Keywords

PEPTIDE BINDING PROTEIN / 14-3-3 / brassinosteroid / transcription factor / BZR1 / phosphopeptide / signal transduction / plant growth

Function / homology

Function and homology information

response to freezing / plant ovule development / seed development / brassinosteroid mediated signaling pathway / plant-type cell wall / response to cold / positive regulation of protein catabolic process / DNA-binding transcription factor activity / negative regulation of DNA-templated transcription / DNA-templated transcription ...
Similarity search - Function

Biological species

Arabidopsis thaliana (thale cress)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.69 Å

Authors

Hothorn, M. / Obergfell, E.

Funding support

Switzerland, 1items

Citation

Journal: Plant Cell.Physiol. / Year: 2024
Title: Mechanistic insights into the function of 14-3-3 proteins as negative regulators of brassinosteroid signaling in Arabidopsis.
Authors: Obergfell, E. / Hohmann, U. / Moretti, A. / Chen, H. / Hothorn, M.

History

Deposition	Oct 12, 2023	Deposition site: PDBE / Processing site: PDBE
Revision 1.0	Oct 25, 2023	Provider: repository / Type: Initial release
Revision 1.1	Jun 5, 2024	Group: Database references / Category: citation / citation_author Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	8qt5.cif.gz	1.2 MB	Display	PDBx/mmCIF format
PDB format	pdb8qt5.ent.gz	843.9 KB	Display	PDB format
PDBx/mmJSON format	8qt5.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Summary document	8qt5_validation.pdf.gz	497.9 KB	Display	wwPDB validaton report
Full document	8qt5_full_validation.pdf.gz	520.7 KB	Display
Data in XML	8qt5_validation.xml.gz	56.1 KB	Display
Data in CIF	8qt5_validation.cif.gz	74.6 KB	Display
Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/qt/8qt5 ftp://data.pdbj.org/pub/pdb/validation_reports/qt/8qt5	HTTPS FTP

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Related structure data

Related structure data	8qtcC 8qtfC 8qttC C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#222 - Jun 2018 Proteins and Nanoparticles similarity (8)

Deposited unit

A: 14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

B: 14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

C: 14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

D: 14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

E: 14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

F: 14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

G: 14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

hetero molecules

202 kDa, 7 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

A: 14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

B: 14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

hetero molecules

defined by author&software
Evidence: light scattering
57.7 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

C: 14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

D: 14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

defined by author&software
57.6 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

E: 14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

F: 14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

hetero molecules

defined by author&software
57.7 kDa, 2 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

G: 14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1

defined by author&software
28.8 kDa, 1 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	176.052, 176.052, 172.031
Angle α, β, γ (deg.)	90.000, 90.000, 120.000
Int Tables number	152
Space group name H-M	P3₁21
Space group name Hall	P3₁2"
Symmetry operation	#1: x,y,z #2: -y,x-y,z+1/3 #3: -x+y,-x,z+2/3 #4: x-y,-y,-z+2/3 #5: -x,-x+y,-z+1/3 #6: y,x,-z

#1: Protein	14-3-3-like protein G-BOX factor 14 lambda,Protein BRASSINAZOLE-RESISTANT 1 / Protein BIN2 SUBSTRATE 2 Mass: 28813.428 Da / Num. of mol.: 7 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Strain: Col0 / Gene: GRF6, BZR1, BIS2, At1g75080, F9E10.7 / Plasmid: pMH-HStrxT / Production host: Escherichia coli BL21 (bacteria) / Variant (production host): DE3 RIL / References: UniProt: P48349, UniProt: Q8S307
#2: Chemical	ChemComp-ACT / ACETATE ION Mass: 59.044 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C₂H₃O₂
#3: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 22 / Source method: isolated from a natural source / Formula: H₂O
Has ligand of interest	N

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 3.78 Å³/Da / Density % sol: 67.48 %
Crystal grow	Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 5.5 Details: 22 % [w/v] PEG 10,000, 0.1 M ammonium acetate, 0.1 M Bis-Tris [pH 5.5]

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Data collection

Diffraction	Mean temperature: 100 K / Serial crystal experiment: N
Diffraction source	Source: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 1.03320713866 Å
Detector	Type: DECTRIS PILATUS 2M-F / Detector: PIXEL / Date: May 13, 2017
Radiation	Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength	Wavelength: 1.03320713866 Å / Relative weight: 1
Reflection	Resolution: 2.69→74.92 Å / Num. obs: 84698 / % possible obs: 98.5 % / Observed criterion σ(I): -3 / Redundancy: 20.2 % / B_iso Wilson estimate: 86.23 Å² / CC_1/2: 1 / R_rim(I) all: 0.111 / Net I/σ(I): 19.54
Reflection shell	Resolution: 2.69→2.85 Å / Redundancy: 20.8 % / Mean I/σ(I) obs: 1.09 / Num. unique obs: 13110 / CC_1/2: 0.536 / R_rim(I) all: 3.037 / % possible all: 95.2

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Processing

Software

Name	Version	Classification
PHENIX	1.20.1_4487	refinement
XDS		data reduction
XSCALE		data scaling
PHASER		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT / Resolution: 2.69→74.92 Å / SU ML: 0.4775 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 29.8934
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2

	R_factor	Num. reflection	% reflection
R_free	0.2616	4232	5 %
R_work	0.2407	80343	-
obs	0.2417	84575	98.33 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso mean: 117.08 Å²

Refinement step

Cycle: LAST / Resolution: 2.69→74.92 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	13613	0	8	22	13643

Refine LS restraints

Refine-ID	Type	Dev ideal	Number
X-RAY DIFFRACTION	f_bond_d	0.002	13835
X-RAY DIFFRACTION	f_angle_d	0.4057	18652
X-RAY DIFFRACTION	f_chiral_restr	0.0286	2089
X-RAY DIFFRACTION	f_plane_restr	0.0035	2392
X-RAY DIFFRACTION	f_dihedral_angle_d	10.6286	5215

LS refinement shell

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Refine-ID	% reflection obs (%)
2.69-2.72	0.4753	116	0.4541	2154	X-RAY DIFFRACTION	79.54
2.72-2.75	0.4615	139	0.4216	2646	X-RAY DIFFRACTION	98.38
2.75-2.78	0.3733	139	0.3965	2640	X-RAY DIFFRACTION	98.48
2.78-2.82	0.3659	140	0.3728	2647	X-RAY DIFFRACTION	98.17
2.82-2.85	0.4001	142	0.3443	2694	X-RAY DIFFRACTION	98.68
2.85-2.89	0.3631	139	0.3387	2655	X-RAY DIFFRACTION	98.62
2.89-2.94	0.3833	142	0.3263	2685	X-RAY DIFFRACTION	98.78
2.94-2.98	0.3522	137	0.3261	2615	X-RAY DIFFRACTION	98.57
2.98-3.03	0.3454	140	0.3201	2656	X-RAY DIFFRACTION	98.45
3.03-3.08	0.3266	142	0.3222	2688	X-RAY DIFFRACTION	98.64
3.08-3.13	0.3196	140	0.3124	2656	X-RAY DIFFRACTION	98.73
3.13-3.18	0.346	141	0.3339	2679	X-RAY DIFFRACTION	98.84
3.19-3.25	0.4001	142	0.3504	2694	X-RAY DIFFRACTION	98.75
3.25-3.31	0.3688	140	0.3428	2663	X-RAY DIFFRACTION	98.94
3.31-3.38	0.3736	141	0.3074	2682	X-RAY DIFFRACTION	99.02
3.38-3.46	0.2866	141	0.2945	2679	X-RAY DIFFRACTION	99.09
3.46-3.55	0.2917	141	0.2843	2684	X-RAY DIFFRACTION	99.16
3.55-3.65	0.2685	141	0.2638	2685	X-RAY DIFFRACTION	99.02
3.65-3.75	0.2262	142	0.2453	2691	X-RAY DIFFRACTION	98.92
3.75-3.87	0.2721	141	0.2489	2684	X-RAY DIFFRACTION	99.33
3.87-4.01	0.2577	143	0.2476	2716	X-RAY DIFFRACTION	99
4.01-4.17	0.2756	144	0.237	2724	X-RAY DIFFRACTION	99.51
4.17-4.36	0.2409	141	0.2143	2685	X-RAY DIFFRACTION	99.19
4.36-4.59	0.2339	143	0.2058	2718	X-RAY DIFFRACTION	99.37
4.59-4.88	0.227	143	0.2165	2717	X-RAY DIFFRACTION	99.41
4.88-5.26	0.2411	144	0.215	2728	X-RAY DIFFRACTION	99.24
5.26-5.79	0.2413	144	0.2342	2736	X-RAY DIFFRACTION	99.45
5.79-6.62	0.2475	146	0.2386	2775	X-RAY DIFFRACTION	99.62
6.62-8.34	0.2389	146	0.1995	2779	X-RAY DIFFRACTION	99.59
8.35-74.92	0.1955	152	0.1728	2888	X-RAY DIFFRACTION	99.28

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	5.16670621726	-1.75320233669	3.64045152964	3.38660109549	-0.302763579718	7.44209563422	0.292626770786	-0.284797285366	-0.43324557043	0.17774526401	0.363853456892	0.980427099588	0.465258868127	-0.486982558744	-0.46021491009	0.743423155991	-0.230407039799	-0.0393862910767	0.735269644621	0.292798165013	0.944845751318	-170.448192566	77.8509734253	22.7363127344
2	4.36410684559	-1.03533233153	1.85907593754	2.01635139862	-2.12484697193	5.88993189597	0.0281105547224	0.174140616125	-0.643601824459	-0.600048921793	0.299100843039	-0.0161598477796	0.473368940105	-0.0447534528238	-0.205134205794	0.821353514101	-0.196040929919	0.0613273064615	0.647854658845	0.0468957064198	0.898347117231	-164.335133625	81.7413966193	19.2170404391
3	4.38938171519	-3.78847302707	4.57182100282	2.76809108172	-3.43548917077	6.2634547191	-0.0154878975523	-1.01821209342	0.863303998188	0.121474780196	-0.174469419864	-0.354646333796	-0.367916117647	0.0890980514583	0.175809899974	0.689883666954	-0.249320241644	0.0670014438424	0.774278945627	-0.0770733696842	0.659133434194	-149.733411381	87.5471496555	29.823966112
4	3.46790001989	-2.22218242811	2.571340317	3.3399895774	-2.02174045919	4.47313033439	0.175249403839	0.337748363204	0.615527663251	-0.386604052628	-0.418406846872	-0.882894877788	0.156737479224	0.951415314644	0.344073733983	0.693152050071	-0.214391590975	0.164931611357	0.75261552263	0.0163668034339	0.75304645183	-144.392423622	84.9886381374	23.0273413778
5	0.364110181737	0.657877857211	0.350610352002	2.67188499597	-1.42034667967	3.85756544579	-0.231532401046	-0.0215575212158	0.172024736467	-0.140495133369	0.126325279237	-0.203869464306	-0.632097289541	0.145489298512	0.0682953221051	0.972701015603	-0.218631037638	0.0763183389658	0.661489107385	0.0112506026458	0.746402951209	-153.584598648	103.113208451	21.7726460998
6	9.89928911777	-1.43292519167	-3.00526718386	3.95060560979	-0.667556183152	7.8678378643	0.0181954812741	2.09273559963	-0.195552731643	-1.24940231926	-0.357710392353	0.977881606109	0.330167433422	-2.65938223565	-0.0585042325185	1.32768392243	0.128288521067	0.0260571882484	1.84108332788	0.101366930734	1.14555845198	-173.910469249	107.992138482	29.703588477
7	2.28989885259	3.60579261828	1.83952483571	6.54584599681	1.29337036022	3.97796973888	1.27834045964	-2.22128733725	-1.08258020289	2.0137268981	-0.815737026449	-0.171767664291	0.37939981705	-0.188498872997	-0.266339418151	1.19602014336	-0.171500038373	0.163443737081	0.928137204441	0.0349156780635	0.668194800875	-158.698137328	105.884525393	35.7477773109
8	5.77897615198	0.221470126114	3.748340325	4.14577792565	2.44678213199	7.81020463605	-0.725714200253	-0.222953027493	-0.0986212273144	-0.260572201319	0.733268866859	-0.58504292131	-0.563011717358	0.621010448893	0.00343595503926	1.01647913837	-0.0505531487089	0.0834642013217	0.685916919466	-0.122046330021	0.619496667094	-146.118038122	105.743847054	32.3514714758
9	3.5628639665	-1.2254726474	0.696764393768	2.67107744981	-0.723569864301	2.13610763314	-0.149686801892	-0.166169594857	-0.287307353478	0.0463120112151	0.19735141203	0.238388282494	0.108886548095	-0.0774472424227	-0.0883879805264	0.706693248687	-0.111054636243	0.129714586823	0.724735288981	0.122553495286	0.769531715283	-153.167379637	62.1371518795	41.6601061723
10	7.81744603272	-0.00766549903473	7.69432503275	2.71916344936	1.41548649648	8.66365903425	0.752270529806	-2.96177716082	-0.480156204845	0.883511036846	-0.719855137196	-0.109976978585	1.06555695686	-0.567148000962	0.0574680740354	1.54833346346	-0.0407500306551	0.327852947295	1.62165805829	0.275186675711	0.992978199017	-159.703990909	57.8037630191	64.5163282883
11	4.04676500881	-1.66485845721	4.37211414546	7.88990653453	1.15159236	5.93004624285	-0.960095622999	-2.88001260716	1.97395992808	2.0897655566	0.387581681115	-1.85592361	-0.492109968481	0.0059808165956	0.0645055801233	1.50203486589	0.154977822412	-0.551068318942	1.59260922578	-0.478563810482	1.89708058342	-144.80157471	67.3456655157	69.4911304458
12	5.63963805985	-0.729072743409	6.9932199854	5.95787782257	0.215878219472	9.26003320574	-0.122315023707	-3.44706105422	1.86524439526	0.911013915091	0.133457033914	0.124347723137	-1.34084036419	-1.28202223893	0.21029088745	1.26546671073	0.140073904516	0.315751764122	1.26826625223	-0.0444597269305	0.899660778499	-159.721707547	67.576142052	61.0772103024
13	5.33632360531	2.34061837745	-5.25749814483	4.12220346802	-1.97817695776	5.19621489835	0.20219097256	0.201152955084	0.15772731545	-0.113810871648	0.108565312077	0.204434154171	-0.649981893158	-1.02767292762	-0.271516003691	1.13697859967	0.072846326433	0.087903016524	1.23691860032	0.179627005468	0.839926847671	-166.705361843	66.5683841791	54.0302219617
14	2.02754149349	0.226281724753	-0.27788347478	3.91097908834	0.925692137794	2.72773585095	-0.0476482645798	0.526807328319	0.206642253943	-0.394664545943	0.00202595847452	0.601645387649	0.000486010044809	0.078990420613	0.0857192608378	0.525242359966	0.100626426853	-0.0710545203527	0.618993284822	0.119190630489	0.783224561362	-145.180492692	34.0470631847	34.7904939591
15	6.58455389857	2.20425234353	-1.28294945397	6.68321217044	0.654872414848	2.45676982976	0.00830372238693	0.340824054909	0.243013552936	-0.211659338665	-0.0450112226936	-0.22587297086	0.0791300203951	0.20339135714	-0.17084284668	0.712950408597	0.0250381168141	0.0504991823157	0.917561786364	0.0633672649681	0.81332580072	-125.463412419	33.0385043252	28.9679301448
16	1.6938432623	0.954063258906	0.0718969736046	2.87069296931	0.597169618832	3.62943334898	-0.0406077517067	0.34162294831	0.0148087555137	-0.32321439069	0.0375242063217	1.10137121365	-0.196899000485	-0.225067901531	0.0782776308723	0.552883147581	0.104696842004	-0.164789522646	0.724763827292	0.00676105312034	1.20879400271	-158.882616297	10.7924849459	34.1866330664
17	3.5707976676	-0.881909073686	0.320068915577	5.44242214364	0.88286287639	6.13655423782	0.146001742373	0.511066941183	-0.28409701824	-0.204562913989	-0.171237462325	0.678835195301	0.407871207987	0.348831849974	0.00255003249548	0.584701437914	0.0219841857176	-0.219422806436	0.624305460276	-0.025067154362	0.969214423383	-153.889080364	-8.88771017926	32.8943384437
18	4.13592363703	2.51218796803	-4.25383214381	4.2125121927	-2.27268722847	7.63387747279	0.156385870001	0.384311170011	1.01324751978	-0.0719180948188	-0.0341944975972	0.662799210429	-0.168609832324	-0.178020867605	-0.533389205959	0.793318525204	0.0716027402939	0.165333548355	0.738409387376	-0.0244741542387	0.895731689245	-113.095301699	37.7845198787	64.9046366126
19	0.900898803676	-0.908604686045	-1.7240212917	3.71607081256	1.03545958044	3.19884262472	-0.186550112581	-0.0168848331821	0.0341661644949	0.766083939999	0.140486734404	-0.110382320845	0.11322467744	0.331734863978	0.115973101629	0.551463492731	0.12197851727	0.0186497050064	0.764488229629	-0.00518523016081	0.608236307409	-102.018983241	25.8300096113	66.640937552
20	3.64753734802	1.33769033293	-1.36628366392	0.699349482286	0.22886366095	3.71818126501	-0.481470312821	0.17861658283	-0.510415874297	-0.0019217374647	0.379758764161	-0.154180784611	1.5033926091	0.335993338922	0.263487817496	1.15582732631	0.388043197945	0.116236944716	0.851235405864	-0.0379948891634	0.637672311465	-99.6275870754	11.0387339404	65.508491811
21	2.94344573468	2.07484607537	-1.12232997467	2.18411719197	-1.31610934292	5.07766405642	0.0259487493158	0.172493833218	-0.169265912037	0.580269140222	0.0112172386455	0.127513108686	-0.141184276757	1.03968316714	-0.102773285497	0.620504623686	0.201043559355	0.0352062024328	1.01446539759	0.00833156939597	0.614687462189	-92.6419251085	28.9634194108	59.2085218964
22	4.3012298945	3.48136168875	-1.91955464594	9.49223894155	0.993228263965	4.78868485578	-0.596053724058	0.726836918763	-0.399200339236	0.109284844399	0.897254019009	-0.80133901425	0.74020056303	1.27473994105	-0.361655606859	0.799945465028	0.287980263167	-0.00439706734041	1.41538337978	-0.0896761861484	0.742314974261	-84.1825626552	24.9880981617	57.0189425315
23	0.891647280813	1.97041251306	-0.282891563486	6.30993333155	0.169928767307	4.73314600007	-0.202238430793	-0.788646878569	-0.0279908977293	0.303814441587	0.0494193975086	-0.192980200128	0.152870271535	1.00920454917	-0.0886637683512	0.685201021292	0.198905754777	0.011679352588	1.59195500721	-0.0675094946435	0.746995787248	-82.4966620366	29.2790735375	66.7053705241
24	2.3058291267	2.88464830924	0.11478657696	5.87842255338	-0.285902888945	5.87737305312	0.151711206641	-0.105517574398	-0.298832092996	-0.224171353345	0.169660180146	-1.54406224536	-0.446735773678	0.940325524343	-0.0257884014708	0.664828221564	0.0595012296814	0.0265555897773	1.88376822612	-0.0425986214455	0.747710416781	-74.9916045452	35.03176974	70.5570737426
25	2.65009337214	0.945367375992	-1.5010626698	7.11716749117	1.32310662143	4.30771776936	0.0899336854056	-0.938407804343	-0.283051497222	1.01050516893	0.255574985981	-0.548712722038	0.162626412329	0.553474540469	-0.327206329535	0.847102681113	-0.00962711327343	-0.0806530016646	1.60661254752	-0.0626937575399	0.663042256184	-81.8693605585	33.176326447	76.9781462099
26	3.33543605049	0.340292089965	1.2424405258	1.87980586797	-1.64114809999	7.45344868834	0.130650213708	-1.10259326811	-0.289602926953	0.013263624455	0.0381172918886	0.628263904789	-0.201985663347	-1.14714460092	0.284459493421	0.676331590341	-0.199312466105	0.0682826208922	0.747155162892	0.0102033995362	0.668012526994	-83.7518548039	22.6502427302	75.8430250966
27	5.5063409761	0.718932886972	-0.247323273676	5.66479611597	-2.46142737202	4.86252153278	0.180961710981	-0.142035590533	-0.965105196339	-0.517979833708	-0.0582814000879	0.138976185268	1.00756514123	0.160185125229	-0.0404852182629	1.42819188387	0.141480000264	0.205728282059	0.758122842244	0.0873121417707	0.962964875139	-112.883833184	3.43052706512	78.6889545471
28	4.74743884303	-2.08983577892	1.5643342398	3.58491851926	-5.2823495467	8.13431438409	0.133031206377	-0.556321311425	0.247234638023	0.525995000471	0.00848497511903	-0.36509750482	-0.137081962873	-0.0614910446536	-0.197145870495	0.721533539438	-0.0681413367323	0.138409651772	0.647206527466	-0.140152007797	0.577438027359	-117.903862422	22.5922241145	81.5870940958
29	3.6611119373	0.365302896589	-0.0107121527416	6.97033303176	-5.14065770383	9.18751642697	-0.415682641465	-0.346467017192	-0.26779980357	0.187750908591	0.949691679356	0.758190817147	0.925171323086	-1.89852401375	-0.595623038254	0.850907516394	-0.031524496831	0.241839504697	0.937279014246	0.0276207184697	0.756137236437	-127.380705295	18.9426910182	86.3624543289
30	6.82867407504	1.02492186257	3.2630221682	4.52117428746	2.87876208642	5.27290686298	-0.451310618472	-0.681873212098	-0.388804253464	0.951480750284	0.0171339384894	0.277694629974	-1.07069138118	-1.62507027616	0.54254519271	1.93922485169	0.397549434597	0.17662638557	1.35284772544	-0.000301564385591	0.821528225512	-119.914473918	20.004860414	105.752208599
31	6.62084055201	-7.03144772931	-5.3733067537	8.26995680813	5.840351901	4.43079263034	-1.92317518817	-1.22228287495	0.340971836197	3.92231414502	2.33449560299	-0.82495396935	-0.798500448255	-0.34372149602	-0.0232830061089	1.66219157561	0.349152916015	0.00692807551755	1.33030543018	-0.0390940775181	0.799099607556	-122.620464535	29.5760608646	95.929566775
32	3.51940057156	1.63131585781	2.91762577543	3.09000757794	0.599900101841	5.4439795975	1.13303419509	-1.8572220918	1.0777527268	-0.446081796758	-1.66961504585	0.705881135548	0.451209773119	-0.866505087568	1.01264973817	1.59558172412	0.2706122659	-0.0950817897822	3.1092033899	-0.546419968447	2.48817385751	-111.663410258	64.5849438286	88.7933214076
33	2.2687273776	0.356251034944	-2.59953233467	0.606344272197	-0.442621702419	2.60062691486	-0.78419309986	-0.314589585125	1.09564447882	-0.0340085918003	0.0487931773201	-0.513547577832	-0.370102322255	2.22484885545	0.737680843166	2.24571343983	-0.278597546444	-0.188785958381	2.87160675753	-0.263037948003	1.72004417892	-109.527749806	45.7168961518	95.0281680934

Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

ID	Refine TLS-ID	Selection details	Auth asym-ID	Label asym-ID	Auth seq-ID	Label seq-ID
1	1	chain 'A' and (resid 4 through 19 )	A	A	4 - 19	1 - 16
2	2	chain 'A' and (resid 20 through 44 )	A	A	20 - 44	17 - 41
3	3	chain 'A' and (resid 45 through 78 )	A	A	45 - 78	42 - 75
4	4	chain 'A' and (resid 79 through 120 )	A	A	79 - 120	76 - 117
5	5	chain 'A' and (resid 121 through 210 )	A	A	121 - 210	118 - 207
6	6	chain 'A' and (resid 211 through 219 )	A	A	211 - 219	208 - 216
7	7	chain 'A' and (resid 220 through 239 )	A	A	220 - 239	217 - 236
8	8	chain 'A' and (resid 240 through 1175 )	A	A	240 - 1175	237 - 248
9	9	chain 'B' and (resid 4 through 193 )	B	C	4 - 193	1 - 190
10	10	chain 'B' and (resid 194 through 211 )	B	C	194 - 211	191 - 208
11	11	chain 'B' and (resid 212 through 219 )	B	C	212 - 219	209 - 216
12	12	chain 'B' and (resid 220 through 240 )	B	C	220 - 240	217 - 237
13	13	chain 'B' and (resid 241 through 1175 )	B	C	241 - 1175	238 - 247
14	14	chain 'C' and (resid 4 through 189 )	C	D	4 - 189	1 - 186
15	15	chain 'C' and (resid 190 through 1175 )	C	D	190 - 1175	187 - 246
16	16	chain 'D' and (resid 4 through 112 )	D	E	4 - 112	1 - 109
17	17	chain 'D' and (resid 113 through 1175 )	D	E	113 - 1175	110 - 247
18	18	chain 'E' and (resid 4 through 19 )	E	F	4 - 19	1 - 16
19	19	chain 'E' and (resid 20 through 78 )	E	F	20 - 78	17 - 75
20	20	chain 'E' and (resid 79 through 112 )	E	F	79 - 112	76 - 109
21	21	chain 'E' and (resid 113 through 143 )	E	F	113 - 143	110 - 140
22	22	chain 'E' and (resid 144 through 168 )	E	F	144 - 168	141 - 165
23	23	chain 'E' and (resid 169 through 193 )	E	F	169 - 193	166 - 190
24	24	chain 'E' and (resid 194 through 214 )	E	F	194 - 214	191 - 211
25	25	chain 'E' and (resid 215 through 242 )	E	F	215 - 242	212 - 239
26	26	chain 'E' and (resid 243 through 1175 )	E	F	243 - 1175	240 - 247
27	27	chain 'F' and (resid 4 through 44 )	F	G	4 - 44	1 - 41
28	28	chain 'F' and (resid 45 through 77 )	F	G	45 - 77	42 - 74
29	29	chain 'F' and (resid 78 through 193 )	F	G	78 - 193	75 - 190
30	30	chain 'F' and (resid 194 through 239 )	F	G	194 - 239	191 - 236
31	31	chain 'F' and (resid 240 through 1175 )	F	G	240 - 1175	237 - 245
32	32	chain 'G' and (resid 4 through 112 )	G	I	4 - 112	1 - 109
33	33	chain 'G' and (resid 113 through 1175 )	G	I	113 - 1175	110 - 242

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