[English] 日本語
![](img/lk-miru.gif)
- PDB-8qpj: FAD-independent Methylene-Tetrahydrofolate Reductase Mutant E9Q f... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 8qpj | ||||||
---|---|---|---|---|---|---|---|
Title | FAD-independent Methylene-Tetrahydrofolate Reductase Mutant E9Q from Mycobacterium hassiacum | ||||||
![]() | Methylenetetrahydrofolate reductase (NAD(P)H) | ||||||
![]() | OXIDOREDUCTASE / Methylenetetrahydrofolate reductase / methylene-tetrahydrofolate reductase | ||||||
Function / homology | Mycobacterial methylenetetrahydrofolate reductase / Methylenetetrahydrofolate reductase (NAD(P)H)![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gehl, M. / Demmer, U. / Ermler, U. / Shima, S. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: Mutational and structural studies of ( beta alpha ) 8 -barrel fold methylene-tetrahydropterin reductases utilizing a common catalytic mechanism. Authors: Gehl, M. / Demmer, U. / Ermler, U. / Shima, S. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 337.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 276.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 8qplC ![]() 8qpmC ![]() 8qq8C C: citing same article ( |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 32836.695 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.03 Å3/Da / Density % sol: 39.5 % |
---|---|
Crystal grow | Temperature: 281 K / Method: vapor diffusion, sitting drop Details: 25 % PEG MME 5000 10 mg/ml Mfr_E9Q 2 mM NAD+ 2 mM CH3-H4F |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Nov 25, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→45.8 Å / Num. obs: 51871 / % possible obs: 98.7 % / Redundancy: 3.3 % / CC1/2: 0.997 / Rmerge(I) obs: 0.088 / Net I/σ(I): 8.17 |
Reflection shell | Resolution: 1.75→1.813 Å / Rmerge(I) obs: 1.05 / Num. unique obs: 5183 / CC1/2: 0.431 |
-
Processing
Software |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.75→45.8 Å
|