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- PDB-8qfb: Crystal structure of human MPP8 C-terminal region (residues 565-860) -

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Basic information

Entry
Database: PDB / ID: 8qfb
TitleCrystal structure of human MPP8 C-terminal region (residues 565-860)
ComponentsM-phase phosphoprotein 8
KeywordsTRANSCRIPTION / Transcriptional regulation / epigenetic silencing / antiviral response / genome stability / histone H3 lysine 9 methylation (H3K9me3) / transposable element (TE) / long interspersed nuclear element-1 (LINE-1) / RNA-binding protein / Human silencing hub (HUSH) complex
Function / homology
Function and homology information


constitutive heterochromatin formation / transposable element silencing by heterochromatin formation / histone H3K9me2/3 reader activity / negative regulation of gene expression via chromosomal CpG island methylation / negative regulation of gene expression, epigenetic / : / heterochromatin / Regulation of endogenous retroelements by the Human Silencing Hub (HUSH) complex / nucleosome / chromatin binding ...constitutive heterochromatin formation / transposable element silencing by heterochromatin formation / histone H3K9me2/3 reader activity / negative regulation of gene expression via chromosomal CpG island methylation / negative regulation of gene expression, epigenetic / : / heterochromatin / Regulation of endogenous retroelements by the Human Silencing Hub (HUSH) complex / nucleosome / chromatin binding / nucleolus / nucleoplasm / nucleus / plasma membrane / cytoplasm / cytosol
Similarity search - Function
: / Chromo domain, conserved site / Chromo domain signature. / Chromo domain / Chromo (CHRromatin Organisation MOdifier) domain / Chromo and chromo shadow domain profile. / Chromo/chromo shadow domain / Chromatin organization modifier domain / Chromo-like domain superfamily / Ankyrin repeat ...: / Chromo domain, conserved site / Chromo domain signature. / Chromo domain / Chromo (CHRromatin Organisation MOdifier) domain / Chromo and chromo shadow domain profile. / Chromo/chromo shadow domain / Chromatin organization modifier domain / Chromo-like domain superfamily / Ankyrin repeat / Ankyrin repeats (3 copies) / Ankyrin repeat profile. / Ankyrin repeat region circular profile. / ankyrin repeats / Ankyrin repeat / Ankyrin repeat-containing domain superfamily
Similarity search - Domain/homology
M-phase phosphoprotein 8
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.04 Å
AuthorsPrigozhin, D.M. / Modis, Y.
Funding support United Kingdom, 2items
OrganizationGrant numberCountry
Wellcome Trust217191/Z/19/Z United Kingdom
Wellcome TrustMX15916 United Kingdom
CitationJournal: J.Mol.Biol. / Year: 2024
Title: Structure and Methyl-lysine Binding Selectivity of the HUSH Complex Subunit MPP8.
Authors: Nikolopoulos, N. / Oda, S.I. / Prigozhin, D.M. / Modis, Y.
History
DepositionSep 4, 2023Deposition site: PDBE / Processing site: PDBE
Revision 1.0Sep 11, 2024Provider: repository / Type: Initial release
Revision 1.1Nov 6, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature
Revision 1.2Dec 25, 2024Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID / _citation_author.name

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
B: M-phase phosphoprotein 8
A: M-phase phosphoprotein 8
C: M-phase phosphoprotein 8
D: M-phase phosphoprotein 8
E: M-phase phosphoprotein 8
F: M-phase phosphoprotein 8
G: M-phase phosphoprotein 8
H: M-phase phosphoprotein 8


Theoretical massNumber of molelcules
Total (without water)295,3608
Polymers295,3608
Non-polymers00
Water00
1
B: M-phase phosphoprotein 8


Theoretical massNumber of molelcules
Total (without water)36,9201
Polymers36,9201
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
A: M-phase phosphoprotein 8


Theoretical massNumber of molelcules
Total (without water)36,9201
Polymers36,9201
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: M-phase phosphoprotein 8


Theoretical massNumber of molelcules
Total (without water)36,9201
Polymers36,9201
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: M-phase phosphoprotein 8


Theoretical massNumber of molelcules
Total (without water)36,9201
Polymers36,9201
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
E: M-phase phosphoprotein 8


Theoretical massNumber of molelcules
Total (without water)36,9201
Polymers36,9201
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
F: M-phase phosphoprotein 8


Theoretical massNumber of molelcules
Total (without water)36,9201
Polymers36,9201
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
7
G: M-phase phosphoprotein 8


Theoretical massNumber of molelcules
Total (without water)36,9201
Polymers36,9201
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
8
H: M-phase phosphoprotein 8


Theoretical massNumber of molelcules
Total (without water)36,9201
Polymers36,9201
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)102.556, 179.531, 228.076
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and (resid 567 through 570 or (resid 571...
d_2ens_1(chain "B" and (resid 567 through 571 or (resid 572...
d_3ens_1(chain "C" and (resid 567 through 574 or (resid 575...
d_4ens_1(chain "D" and (resid 567 through 570 or (resid 571...
d_5ens_1(chain "E" and (resid 567 through 570 or (resid 571...
d_6ens_1(chain "F" and (resid 567 through 570 or (resid 571...
d_7ens_1(chain "G" and (resid 567 through 570 or (resid 571...
d_8ens_1(chain "H" and (resid 567 through 570 or (resid 571...

NCS domain segments:

Ens-ID: ens_1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11SERSERASPASPAB567 - 73643 - 212
d_12PHEPHELEULEUAB738 - 859214 - 335
d_21SERSERASPASPBA567 - 73643 - 212
d_22PHEPHELEULEUBA738 - 859214 - 335
d_31SERSERASPASPCC567 - 73643 - 212
d_32PHEPHELEULEUCC738 - 859214 - 335
d_41SERSERASPASPDD567 - 73643 - 212
d_42PHEPHELEULEUDD738 - 859214 - 335
d_51SERSERASPASPEE567 - 73643 - 212
d_52PHEPHELEULEUEE738 - 859214 - 335
d_61SERSERASPASPFF567 - 73643 - 212
d_62PHEPHELEULEUFF738 - 859214 - 335
d_71SERSERASPASPGG567 - 73643 - 212
d_72PHEPHELEULEUGG738 - 859214 - 335
d_81SERSERASPASPHH567 - 73643 - 212
d_82PHEPHELEULEUHH738 - 859214 - 335

NCS oper:
IDCodeMatrixVector
1given(-0.5616031734, -0.820683964369, -0.105260183616), (-0.827205498162, 0.559710119578, 0.0495544735884), (0.018246628122, 0.114901752248, -0.993209266918)-58.2159356212, -29.4182918616, -57.7027656365
2given(0.220679673956, 0.694940414445, -0.684367081232), (0.700320035883, -0.601272369367, -0.384738073468), (-0.678861052678, -0.394372106274, -0.619369286412)-74.0226329233, 63.4571676667, -68.6403352836
3given(-0.728614031889, 0.111755286267, 0.675745772111), (0.100870471692, -0.958332685321, 0.267251963857), (0.677456080108, 0.262886325692, 0.686981833303)-68.8501489585, 62.5855964922, 17.8576271824
4given(0.251230573519, -0.492727391129, 0.833128391642), (0.420904530019, -0.719480199655, -0.552437886928), (0.871620660344, 0.489456801297, 0.0266357677958)-2.78515912715, -0.00261453072001, 58.3895610614
5given(0.239730839278, -0.385357315948, -0.891082972424), (0.385067836621, -0.804820771699, 0.451648410427), (-0.891208104709, -0.451401444932, -0.04455165109)-53.3385107876, 28.3039833151, -6.7005329827
6given(0.355571162758, 0.74983984647, 0.557951030881), (0.773209062547, -0.571365440042, 0.275116847033), (0.525087510621, 0.333589176341, -0.782944038625)15.0232299813, -5.67510988419, -30.8653870574
7given(-0.827348390809, 0.181443301463, -0.531575929271), (0.0434265122956, -0.922892498018, -0.382601065253), (-0.560007837673, -0.339628864287, 0.755674173363)-59.3963804022, -50.3821161492, -24.6895062515

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Components

#1: Protein
M-phase phosphoprotein 8 / Two hybrid-associated protein 3 with RanBPM / Twa3


Mass: 36920.055 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Details: Human MPP8 residues 546-860 with N-terminal hexahistidine tag
Source: (gene. exp.) Homo sapiens (human) / Gene: MPHOSPH8, MPP8 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: Q99549
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.56 Å3/Da / Density % sol: 65.4 % / Description: Prismatic, needles
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: 12.5% (w/v) PEG 4000, 20% (v/v) 1,2,6-hexanetriol, 0.5 mM Morpheus II Oxometalate Mix, 0.1 M Morpheus II GlyGly/AMPD buffer mix pH 8.
PH range: 7.4 - 9.8

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.9282 Å
DetectorType: DECTRIS EIGER2 XE 9M / Detector: PIXEL / Date: Sep 19, 2016 / Details: torroidal mirror
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9282 Å / Relative weight: 1
ReflectionResolution: 3→141.03 Å / Num. obs: 85128 / % possible obs: 100 % / Redundancy: 13.2 % / Biso Wilson estimate: 100.74 Å2 / CC1/2: 0.999 / CC star: 1 / Rmerge(I) obs: 0.081 / Rpim(I) all: 0.023 / Rrim(I) all: 0.085 / Net I/σ(I): 14.8
Reflection shellResolution: 3→3.08 Å / Redundancy: 11.2 % / Rmerge(I) obs: 2.36 / Mean I/σ(I) obs: 1.2 / Num. unique obs: 6191 / CC1/2: 0.744 / Rpim(I) all: 0.725 / Rrim(I) all: 2.47 / % possible all: 100

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
xia2data reduction
PHASER1.2phasing
Aimless0.5.27data scaling
Coot0.9model building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.04→93.54 Å / SU ML: 0.6377 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 38.0736
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2947 4134 5.12 %
Rwork0.2555 76617 -
obs0.2575 80751 98.83 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 130.53 Å2
Refinement stepCycle: LAST / Resolution: 3.04→93.54 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms17740 0 0 0 17740
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00518051
X-RAY DIFFRACTIONf_angle_d0.845824559
X-RAY DIFFRACTIONf_chiral_restr0.04552929
X-RAY DIFFRACTIONf_plane_restr0.00693215
X-RAY DIFFRACTIONf_dihedral_angle_d4.99642494
Refine LS restraints NCS
Ens-IDDom-IDAsym-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2BAX-RAY DIFFRACTIONTorsion NCS0.630153951603
ens_1d_3BAX-RAY DIFFRACTIONTorsion NCS0.660615825346
ens_1d_4BAX-RAY DIFFRACTIONTorsion NCS0.700909074283
ens_1d_5BAX-RAY DIFFRACTIONTorsion NCS0.745901021617
ens_1d_6BAX-RAY DIFFRACTIONTorsion NCS0.712602852852
ens_1d_7BAX-RAY DIFFRACTIONTorsion NCS0.62493901332
ens_1d_8BAX-RAY DIFFRACTIONTorsion NCS0.686325085697
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
3.04-3.070.54941370.51262361X-RAY DIFFRACTION95.42
3.07-3.110.54081420.50512488X-RAY DIFFRACTION95.64
3.11-3.150.56361440.48852461X-RAY DIFFRACTION97.27
3.15-3.190.55061320.47822446X-RAY DIFFRACTION96.45
3.19-3.230.44921380.43642482X-RAY DIFFRACTION97.25
3.23-3.270.46891310.42412507X-RAY DIFFRACTION97.81
3.27-3.320.44971290.40892532X-RAY DIFFRACTION98.19
3.32-3.370.41251290.3882489X-RAY DIFFRACTION98.46
3.37-3.420.43881620.36112549X-RAY DIFFRACTION98.98
3.42-3.480.40231310.34732488X-RAY DIFFRACTION98.9
3.48-3.540.40771490.3552548X-RAY DIFFRACTION98.76
3.54-3.60.41461260.36212513X-RAY DIFFRACTION98.69
3.6-3.670.37641280.3662572X-RAY DIFFRACTION99.05
3.67-3.750.41091410.34622531X-RAY DIFFRACTION99.15
3.75-3.830.37791430.3172557X-RAY DIFFRACTION99.41
3.83-3.920.32361300.29872555X-RAY DIFFRACTION99.67
3.92-4.020.30871330.28412558X-RAY DIFFRACTION99.67
4.02-4.130.33561400.27852564X-RAY DIFFRACTION99.38
4.13-4.250.28671320.25022580X-RAY DIFFRACTION99.63
4.25-4.380.28921290.24812579X-RAY DIFFRACTION99.63
4.38-4.540.2641280.21252568X-RAY DIFFRACTION99.56
4.54-4.720.27871470.20762593X-RAY DIFFRACTION99.67
4.72-4.940.25211430.21082568X-RAY DIFFRACTION99.89
4.94-5.20.21991390.20632592X-RAY DIFFRACTION99.53
5.2-5.520.25031400.20032580X-RAY DIFFRACTION99.82
5.52-5.950.2641390.22622628X-RAY DIFFRACTION99.86
5.95-6.550.30161400.2532628X-RAY DIFFRACTION99.96
6.55-7.50.28441330.2362644X-RAY DIFFRACTION99.96
7.5-9.440.21511450.19472684X-RAY DIFFRACTION99.96
9.45-93.540.20991540.17922772X-RAY DIFFRACTION98.99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.344857209293.244439517180.6225106424166.63860519012-0.02478437064484.375499235230.09381159399830.1248671603580.45493213461-1.02919184566-0.1532143854232.23601489461-0.515481670504-1.477883467440.1999588132210.9540863844630.267197879116-0.1676382725181.22472272173-0.1901486341611.46705079411-66.03456151395.81485199274-49.4161407605
25.92262388429-1.21813240540.7491567641756.29789023254-1.075643014584.075707224660.4803725599270.482180945360.0665917333636-0.927809290038-0.714629313082-0.5213398923230.3211150855530.1675622937710.2396776911960.9824366524630.3982752837010.2457771339251.02785699246-0.06692523793230.959292103001-37.55075380767.45777463916-47.152882082
33.09361073493-1.03379537001-0.9173337557675.64432424568-1.222409423586.320212751820.3024997846890.208406714568-0.723566547946-0.818273321134-0.65386545832-0.0995321852220.9319870565840.7467607701880.4337972424781.22027226110.5542215795720.3452644359431.159379599450.01751879786250.963117649958-29.6730873822-8.79820965507-46.2150277873
46.900667575452.585500793950.3366731618359.009099529322.615595281824.283947127390.421766637036-0.924763202032-0.111861456067-0.238204141947-0.666926486489-1.7547758422-1.749343838790.2690504771080.3886262819810.8392063632360.0472062830275-0.1530752404570.8545387726650.1664261558641.24804601613-20.895119335234.1252572747-5.27667927465
55.385828324942.401375803770.3306830840217.60883074091.581506507310.5867197671670.4694302088050.122702271588-0.1977134474430.942106450604-0.284982986271-0.2558447740240.07650746636440.128750410485-0.1954066325710.580239052910.193834995980.09143463610010.7636246423560.06108260877870.628253366408-36.745508879615.2798363132-6.10729145779
67.54593213532-0.7115965556830.5082545213098.685516314770.8405470462374.230185101-0.6397571793390.0133222897163-1.07049834942-0.2714401317370.3346783138651.260523349180.200514862984-0.3769617579850.3911575337570.8145379754450.1141764014160.1869594208880.74121858431-0.1224386296490.77585670556-42.8955164825-4.81130684812-16.9208522051
74.36168234244-0.938783227730.1394530561366.452921130330.5005959721366.93272762398-0.262475126793-0.0649429152513-0.7687235595960.2818029174570.0501906229109-0.6723631837181.101932122030.3988470997540.1487806876551.25441337790.3780533065290.1738574304510.763668474003-0.0223911429051.02623298811-33.0524843195-10.6873034331-13.3328003891
86.406892242995.05114549305-4.937795817294.28185567336-4.28175831515.15189592051-0.3250273664121.905291925950.815604837956-1.97312697625-0.166823896212-0.7378182064571.394947816250.1842883197030.3626491782441.937422160880.9047098303980.08566464316182.10490074311-0.02559551018981.2520474122-55.339732261830.5386730807-61.7937833123
93.96848537569-1.89733559879-3.035587410185.957133161473.220209488584.467285727730.4254711352280.892906607467-0.998284866087-0.425556693254-0.7247120655110.868166646675-0.409843618127-0.6220313796270.1552550957381.046929773570.560001791363-0.09796744094051.266890780170.05163291672881.02548177404-69.209061340932.8219639636-39.4053042741
107.64000228952-0.529141989477-3.237467008693.63579843740.571481905685.421079412461.0336702379-0.0197692902218-1.76120588924-0.0867742641706-1.062958777430.286172724388-0.839589626993-0.1115803798880.08771576640561.2716621960.5405812091040.1447792338511.358211758850.3390277057070.560180756513-77.651670223348.1023004236-30.2975836841
116.551930934680.243646224436-1.175280565783.57947223493.619500474323.599484589870.7019818475740.785089439407-1.31904108632-0.564716810115-0.4462934375881.159523675320.344435813346-0.10846546288-0.2536424389711.303906719250.6141735385590.1094084706191.054881962780.08149748825331.37434859498-83.606990293148.1184781397-31.0796877517
125.40958913908-0.5787412346632.228616572656.796863550482.837568496864.357562251890.1257006721410.8520044814580.447181882043-0.1489854072940.005636023823480.815840931554-0.72208712653-0.1220206548110.01639248599941.103903004440.5162437480170.2461513549751.134308418720.2500391406160.89257716169-78.147059203353.646004532-34.6985461453
139.298291880562.77182471980.2019881477932.81333781339-2.479016735478.437526559970.720115022408-0.4427861592-0.01123406063441.36019612308-0.4506734284340.338011162617-0.525118048019-0.23146110472-0.3605736875010.8730672845260.0547376945310.1763916368550.6956796118930.07936759026720.733084067142-51.800862222429.35090059997.28501846048
147.339086836390.552671004358-0.6104699623149.44884054483-0.6030321120236.74550034197-0.09016425162770.3301004454970.5223528842520.7196759964640.1178923211680.370284921153-1.05236716909-0.623674805139-0.03747495976190.5308015999540.1605316386450.09932191834520.6615219744530.1571830309180.541737389539-49.406188311337.1599780545-4.02603641132
154.62154374827-0.5825679401321.910448448066.40750596631-0.2155208757766.0941243299-0.01619358830560.4005127154550.6544370665160.484574418245-0.112960740748-0.370560375976-0.8676539089350.585460090230.0779949623440.5910918392490.09655511800810.2120830416960.7307073902140.2585507451850.858717538566-42.947748391245.3641967688-11.613143634
163.87079557643-3.470173537060.438596772873.19796799725-0.3278322614574.056511010210.664484517871.220012829352.3559642196-0.124665644044-1.22195944407-2.39732716449-1.986530660991.66883594397-1.042225034040.9024789551920.202238765230.6159079445671.299225956960.3997088803530.991135804279-37.263736279252.3357395944-13.8053901986
173.42972931951-3.566215827811.435680072035.47357824234-0.2175576554861.574473436530.9960452190020.7692714850581.41419080126-1.8147837135-0.934530871764-0.534657359193-0.06590242314070.358154876925-0.03003978142681.027437894320.3852193803520.3914956487521.341953017250.589148970481.2466356034-49.899858242958.4949951714-24.2388213514
184.189387809363.873792581711.73394202944.830285445982.061005620253.402721129310.410816359270.6850220350780.8679259335250.2693862850870.133372810292-0.912529292853-0.680595270094-0.192648788909-0.4700930363661.292802985520.3509149583370.1790800819870.8727431155940.3592048612981.66836238012-54.67069844466.8012876152-16.0875170913
194.76944071408-0.5753220845112.844916605029.86808982507-1.063163347048.70408531630.7799538676160.4345657685151.13260139080.482073731362-0.1610107671370.753036541737-0.1784016808350.9152526389-0.3804341653860.6266295072890.3643235811570.3512512048531.130750846840.4365508953371.06770210935-56.279954749161.5397603986-18.4953334062
206.830596387950.777702063219-0.5190959225772.544580134822.699683032213.969784779660.54903931535-1.73214983876-0.0317433025822.5469509119-0.933040610128-1.938033096991.14480157077-0.2084105057430.4676261365722.10022630888-0.549959227682-0.856570172261.250255420090.4888725147161.66907607345-29.3985624178-29.8387468756.1773498368
213.983365056811.51557478319-2.384850272524.065728294640.4890283947749.036375696060.816831091518-0.9874268096860.07667277497850.847814409732-0.971412133327-1.95064624273-0.1936867587450.817661846523-0.07420841272211.12577842196-0.315332443443-0.6337731473811.133572080870.4749051096791.80032198469-25.7736931-24.714041047645.071477714
223.153713186035.59723065724-2.466135632448.65685874562-3.921923123066.4392530549-0.4536787009690.122891639184-1.4088116044-0.9361175710380.00543142573281-1.343720861550.204869311048-0.2090611049920.3155405398170.96770543849-0.114117788118-0.1617259014950.789808859540.07238365988941.40919254674-24.9372324872-15.18232137924.1422667951
237.70387976561-0.731754098362-1.32142699111.59416465341-0.3983240572053.753309515790.2919278277760.1715193540870.2512819896870.0428245269975-0.271380138577-1.64221012024-0.5476744385640.353961311521-0.08521045050881.0972574978-0.388404229596-0.04618830855950.9784745175410.2403735951931.43039524131-17.26834617891.9206200878523.3006108092
248.89979726353-2.506287415736.285404443194.04354525153-0.7372383934095.54684997605-0.124740226483-0.348708660212-0.550856934191-0.0197339008121-0.351467872693-1.51432748759-0.723464082580.3134923771870.3350101753171.11885891991-0.203287825589-0.05618043039110.7175835877140.2070648573851.34261184032-16.25114592010.13697017811724.9953557611
252.474253359121.586318586330.04741639836234.63647229120.005532145104234.31133680893-0.635672486721.30847113298-0.819625308517-2.847357461091.136790141981.56406073542-0.683986927757-1.656487403170.2209085065052.21109874154-1.18293069833-1.464931536581.140635515660.3490426034121.78155747058-66.6787053013-7.082440506310.698672754578
262.325823385973.23762797420.7618499747797.63770267397-0.2602821021314.58269355981-0.295956089416-0.185820811863-0.0408304491741-0.1434034892970.03318673015621.267919388040.561620231716-0.152322193270.2339778472421.00892398165-0.364970106648-0.1510600027420.9706880537280.2349912300710.871394773166-56.4061035662.3517817810225.8011762361
276.32217237173-2.625338916090.02345796877448.993117191310.3682096255912.611127195380.303649628097-0.6746514173270.04095561526660.134962217935-0.243206591860.642043327517-0.7496681596940.3979763687820.02809053541641.11337115307-0.4172920392740.05748508947450.8616836901650.03869787754810.650309805598-45.2308902318.074269404528.2353386305
285.65293589005-6.291974997560.3402105760462.02304815858-3.014907858862.35497648197-0.608568475207-0.5128960033970.6676631551491.490968130710.599444169739-4.05148735565-0.6331394028370.546691022011-0.1495344370980.716157430910.281747320434-0.01413151501751.45454833143-0.3555153160264.0149889892929.5056757751-42.2060690818-26.8740470421
297.09144119542-1.736379492661.931358277397.379049722890.9742419684243.884757751050.1084089302870.7146733064970.873520040112-0.407473063657-0.49480182062-0.87967361654-0.355346963644-0.111470312840.373049596831.192500213560.2475011953980.2369885326220.7846627530770.100093609771.043281052552.50170471915-43.3326421962-40.6734636203
307.27577164664-1.09686984046-0.2810734922087.47176443447-3.071926814016.731673002680.1409889913510.6077004315150.751001052622-0.471997966526-0.539278403806-0.1775759683230.0188607177793-0.4508949518230.3466827407231.2743497160.5622410418430.1609803378141.10781230813-0.05491045982640.874847203199-12.003940085-28.8398310247-41.4509742186
316.095272185522.68762130363-0.7441285126736.022049695122.067780944259.018397279011.134081691391.21470219637-3.341239972220.005643094539240.4235363280541.429118219370.292965333071-1.1993597714-1.537449689891.475424407610.3771460297160.252500846511.341456848470.1474492252472.75760878973-33.2606740522-79.5431199107-28.0362941991
327.637466406062.204880521914.461814630393.10505086266-2.076144950657.181103124780.669209344594-2.24647510426-0.208538341888-0.332901512750.8866817206761.281573707560.461846505165-2.03329904755-1.684050019261.151259385280.2330209563460.3439147080571.341939120190.3400589462651.87781856105-30.4148409194-73.2632720018-18.0559399579
332.154589526060.6384196510091.49265672976.344207719470.4351399894617.20069690291-0.647406236802-0.626953206889-0.8883436738330.3963391711650.3732870296171.995930723290.772002656093-0.1163063501270.261848213751.388235034310.5711945417280.362677459051.080000681950.2167938678931.71487252687-24.4687917483-67.5434181933-20.5235622068
343.03018031175-2.348713967531.295223273618.100673065741.485677813372.10303912688-0.892062312418-0.5266269830550.07850291768362.186933882490.406533141910.47602975803-0.3390807686-0.1204778684990.4018970820781.849812206540.6665084355930.3855464770341.19833731920.1734610804311.22391543581-17.7568338964-49.9720036357-10.0535848565
354.43990469676-0.0610836143564-0.2168729313963.13463500971-0.8173651191014.01896327423-0.470087228064-0.5596105370180.3939649399911.087406070190.02674516343030.734120712599-0.753618140341-0.3643570669260.347624761052.043624623670.6171321168360.3307796806331.04654494212-0.02471159004751.19103033615-24.0324327715-37.799441068-12.3491089435
364.4305029957-2.08632033418-1.51829819845.650625355272.383104402221.140604490170.398763096293-1.165651831711.520864505221.158229604830.6741346543662.00118092559-0.526172510827-0.5490749917270.8205907179922.648067278571.159940236650.2235617137731.18326140563-0.225267915221.21536861864-33.0805597746-31.038070593-15.3975535591
377.418616655771.97625641399-0.7726910626720.886161901872-1.453944097225.57516555983-1.69006284883-0.8782821317490.6759735754151.248024889250.00785387537341.64034091702-0.623849887637-0.283246046980.8247407043312.07265370690.9494978325980.3048965020840.804014724855-0.06491418766291.10833180298-27.7006721536-38.0740044853-12.2075213768
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'B' and (resid 567 through 636 )BA567 - 6361 - 70
22chain 'B' and (resid 637 through 760 )BA637 - 76071 - 194
33chain 'B' and (resid 761 through 860 )BA761 - 860195 - 294
44chain 'A' and (resid 563 through 596 )AB563 - 5961 - 34
55chain 'A' and (resid 597 through 712 )AB597 - 71235 - 150
66chain 'A' and (resid 713 through 760 )AB713 - 760151 - 198
77chain 'A' and (resid 761 through 860 )AB761 - 860199 - 298
88chain 'C' and (resid 567 through 613 )CC567 - 6131 - 47
99chain 'C' and (resid 614 through 737 )CC614 - 73748 - 171
1010chain 'C' and (resid 738 through 760 )CC738 - 760172 - 194
1111chain 'C' and (resid 761 through 790 )CC761 - 790195 - 224
1212chain 'C' and (resid 791 through 860 )CC791 - 860225 - 294
1313chain 'D' and (resid 563 through 613 )DD563 - 6131 - 51
1414chain 'D' and (resid 614 through 656 )DD614 - 65652 - 94
1515chain 'D' and (resid 657 through 688 )DD657 - 68895 - 126
1616chain 'D' and (resid 689 through 712 )DD689 - 712127 - 150
1717chain 'D' and (resid 713 through 760 )DD713 - 760151 - 198
1818chain 'D' and (resid 761 through 840 )DD761 - 840199 - 278
1919chain 'D' and (resid 841 through 860 )DD841 - 860279 - 298
2020chain 'E' and (resid 565 through 597 )EE565 - 5971 - 33
2121chain 'E' and (resid 598 through 636 )EE598 - 63634 - 72
2222chain 'E' and (resid 637 through 760 )EE637 - 76073 - 196
2323chain 'E' and (resid 761 through 823 )EE761 - 823197 - 259
2424chain 'E' and (resid 824 through 860 )EE824 - 860260 - 296
2525chain 'F' and (resid 567 through 613 )FF567 - 6131 - 47
2626chain 'F' and (resid 614 through 760 )FF614 - 76048 - 194
2727chain 'F' and (resid 761 through 860 )FF761 - 860195 - 294
2828chain 'G' and (resid 565 through 597 )GG565 - 5971 - 33
2929chain 'G' and (resid 598 through 760 )GG598 - 76034 - 196
3030chain 'G' and (resid 761 through 859 )GG761 - 859197 - 295
3131chain 'H' and (resid 565 through 597 )HH565 - 5971 - 33
3232chain 'H' and (resid 598 through 613 )HH598 - 61334 - 49
3333chain 'H' and (resid 614 through 656 )HH614 - 65650 - 92
3434chain 'H' and (resid 657 through 760 )HH657 - 76093 - 196
3535chain 'H' and (resid 761 through 809 )HH761 - 809197 - 245
3636chain 'H' and (resid 810 through 823 )HH810 - 823246 - 259
3737chain 'H' and (resid 824 through 860 )HH824 - 860260 - 296

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