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- PDB-8q8o: PA14_16140 protein: the regulator of an operon involved in the bi... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8q8o | ||||||
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Title | PA14_16140 protein: the regulator of an operon involved in the biofilm formation in PA14 P. aeruginosa | ||||||
![]() | DUF2170 family protein | ||||||
![]() | CHAPERONE / regulator / biofilm / pseudomonas | ||||||
Function / homology | Protein of unknown function DUF2170 / Uncharacterized protein conserved in bacteria (DUF2170) / Tb-Xo4 / DUF2170 family protein![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Broutin, I. / Phan, G. / Girard, E. | ||||||
Funding support | ![]()
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![]() | ![]() Title: BacA: a possible regulator that contributes to the biofilm formation of Pseudomonas aeruginosa . Authors: Wallart, L. / Ben Mlouka, M.A. / Saffiedine, B. / Coquet, L. / Le, H. / Hardouin, J. / Jouenne, T. / Phan, G. / Kiefer-Meyer, M.C. / Girard, E. / Broutin, I. / Cosette, P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 69.3 KB | Display | ![]() |
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PDB format | ![]() | 50 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
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Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 13.1 KB | Display | |
Data in CIF | ![]() | 16.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 15836.769 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: PA14_16140 / Production host: ![]() ![]() #2: Chemical | ChemComp-GOL / | #3: Chemical | ChemComp-7MT / | #4: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.93 Å3/Da / Density % sol: 58 % / Description: long needle |
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Crystal grow | Temperature: 292 K / Method: vapor diffusion, hanging drop / pH: 4.5 / Details: 32% PEG 200, 0.2M NaCl, 0.1M acetate pH 4.5 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jul 28, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.649 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→46.85 Å / Num. obs: 11686 / % possible obs: 99.7 % / Redundancy: 21.4 % / Biso Wilson estimate: 40.66 Å2 / CC1/2: 0.99 / Rmerge(I) obs: 0.169 / Rpim(I) all: 0.037 / Rrim(I) all: 0.173 / Net I/σ(I): 22.9 |
Reflection shell | Resolution: 2.7→2.83 Å / Rmerge(I) obs: 1.099 / Mean I/σ(I) obs: 5 / Num. unique obs: 1478 / CC1/2: 0.95 / Rpim(I) all: 0.243 / Rrim(I) all: 1.126 / % possible all: 99.3 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Displacement parameters | Biso mean: 46.96 Å2 | ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→46.85 Å
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Refine LS restraints |
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