- PDB-8q59: Crystal structure of metal-dependent class II sulfofructose phosp... -
+
Open data
ID or keywords:
Loading...
-
Basic information
Entry
Database: PDB / ID: 8q59
Title
Crystal structure of metal-dependent class II sulfofructose phosphate aldolase from Yersinia aldovae in complex with sulfofructose phosphate (YaSqiA-Zn-SFP)
Mass: 18.015 Da / Num. of mol.: 208 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interest
Y
-
Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Density Matthews: 3.18 Å3/Da / Density % sol: 61.37 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop Details: enzyme in 50 mM NaPi buffer pH 7.4 soaked with SFP for 30 seconds in a drop with 0.1 uL protein- 0.2 uL mother liquor, with the reservoir solution containing 1.5 M Ammonium sulfate, 0.1 M ...Details: enzyme in 50 mM NaPi buffer pH 7.4 soaked with SFP for 30 seconds in a drop with 0.1 uL protein- 0.2 uL mother liquor, with the reservoir solution containing 1.5 M Ammonium sulfate, 0.1 M BIS-TRIS pH 5.5, 1% PEG 3,350
-
Data collection
Diffraction
Mean temperature: 100 K / Serial crystal experiment: N