Evidence: electron microscopy, elution volume suggests monomer, isothermal titration calorimetry, protein molecule (chain A) binds to 1 adduct cmpd6-AMP
Type
Name
Symmetry operation
Number
identity operation
1_555
x,y,z
1
Buried area
620 Å2
ΔGint
-4 kcal/mol
Surface area
8830 Å2
Method
PISA
Unit cell
Length a, b, c (Å)
126.630, 126.630, 61.590
Angle α, β, γ (deg.)
90.00, 90.00, 120.00
Int Tables number
178
Space group name H-M
P6122
Components on special symmetry positions
ID
Model
Components
1
1
A-593-
HOH
2
1
A-625-
HOH
-
Components
-
Protein , 1 types, 1 molecules A
#1: Protein
Leucine--tRNAligase / Leucyl-tRNA synthetase / LeuRS
Mass: 20012.182 Da / Num. of mol.: 1 / Mutation: deletion 354-477 Source method: isolated from a genetically manipulated source Details: residue 30 (cysteine 243) is a S-mercaptocysteine (denoted CSS in PDB entries) Source: (gene. exp.) Wolbachia endosymbiont strain TRS of Brugia malayi (bacteria) Gene: leuS, Wbm0605 / Plasmid: pET-M11 / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): RIL / References: UniProt: Q5GS31, leucine-tRNA ligase