+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 8p9e | ||||||
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タイトル | Crystal structure of wild type p63-p73 heterotetramer (tetramerisation domain) in complex with darpin 1810 F11 | ||||||
要素 |
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キーワード | DNA BINDING PROTEIN / p63 / p73 / tetramerization domain / darpin / heterotetramer / Structural Genomics / Structural Genomics Consortium / SGC | ||||||
機能・相同性 | 機能・相同性情報 ectoderm and mesoderm interaction / epidermal cell division / cloacal septation / positive regulation of somatic stem cell population maintenance / squamous basal epithelial stem cell differentiation involved in prostate gland acinus development / positive regulation of lung ciliated cell differentiation / prostatic bud formation / negative regulation of mesoderm development / female genitalia morphogenesis / establishment of planar polarity ...ectoderm and mesoderm interaction / epidermal cell division / cloacal septation / positive regulation of somatic stem cell population maintenance / squamous basal epithelial stem cell differentiation involved in prostate gland acinus development / positive regulation of lung ciliated cell differentiation / prostatic bud formation / negative regulation of mesoderm development / female genitalia morphogenesis / establishment of planar polarity / positive regulation of keratinocyte proliferation / negative regulation of keratinocyte differentiation / positive regulation of fibroblast apoptotic process / negative regulation of intracellular estrogen receptor signaling pathway / polarized epithelial cell differentiation / proximal/distal pattern formation / negative regulation of cardiac muscle cell proliferation / positive regulation of cell cycle G1/S phase transition / skin morphogenesis / cranial skeletal system development / sympathetic nervous system development / post-anal tail morphogenesis / embryonic forelimb morphogenesis / Differentiation of keratinocytes in interfollicular epidermis in mammalian skin / embryonic hindlimb morphogenesis / TP53 Regulates Transcription of Death Receptors and Ligands / Activation of PUMA and translocation to mitochondria / hair follicle morphogenesis / : / Regulation of TP53 Activity through Association with Co-factors / positive regulation of Notch signaling pathway / WW domain binding / TP53 Regulates Transcription of Caspase Activators and Caspases / positive regulation of oligodendrocyte differentiation / regulation of epidermal cell division / TP53 regulates transcription of several additional cell death genes whose specific roles in p53-dependent apoptosis remain uncertain / odontogenesis of dentin-containing tooth / epithelial cell development / positive regulation of stem cell proliferation / negative regulation of cellular senescence / TP53 Regulates Transcription of Genes Involved in Cytochrome C Release / keratinocyte proliferation / Pyroptosis / negative regulation of neuron differentiation / intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator / establishment of skin barrier / mismatch repair / positive regulation of osteoblast differentiation / keratinocyte differentiation / MDM2/MDM4 family protein binding / Notch signaling pathway / regulation of mitotic cell cycle / kidney development / transcription corepressor binding / determination of adult lifespan / stem cell proliferation / skeletal system development / promoter-specific chromatin binding / TP53 Regulates Metabolic Genes / RNA polymerase II transcription regulatory region sequence-specific DNA binding / protein tetramerization / positive regulation of apoptotic signaling pathway / intrinsic apoptotic signaling pathway in response to DNA damage / cellular senescence / p53 binding / cell junction / RUNX1 regulates transcription of genes involved in differentiation of HSCs / spermatogenesis / regulation of gene expression / DNA-binding transcription activator activity, RNA polymerase II-specific / DNA-binding transcription factor binding / neuron apoptotic process / RNA polymerase II-specific DNA-binding transcription factor binding / transcription by RNA polymerase II / positive regulation of MAPK cascade / damaged DNA binding / regulation of cell cycle / transcription cis-regulatory region binding / DNA-binding transcription factor activity, RNA polymerase II-specific / chromatin remodeling / positive regulation of apoptotic process / response to xenobiotic stimulus / DNA-binding transcription factor activity / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of cell population proliferation / intracellular membrane-bounded organelle / negative regulation of DNA-templated transcription / DNA damage response / dendrite / chromatin binding / chromatin / regulation of transcription by RNA polymerase II / apoptotic process / protein kinase binding / positive regulation of DNA-templated transcription / Golgi apparatus / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) Lama glama (ラマ) | ||||||
手法 | X線回折 / シンクロトロン / 分子置換 / 解像度: 2.25 Å | ||||||
データ登録者 | Chaikuad, A. / Strubel, A. / Doetsch, V. / Knapp, S. / Structural Genomics Consortium (SGC) | ||||||
資金援助 | 1件
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引用 | ジャーナル: Cell Death Dis / 年: 2023 タイトル: DARPins detect the formation of hetero-tetramers of p63 and p73 in epithelial tissues and in squamous cell carcinoma. 著者: Strubel, A. / Munick, P. / Hartmann, O. / Chaikuad, A. / Dreier, B. / Schaefer, J.V. / Gebel, J. / Osterburg, C. / Tuppi, M. / Schafer, B. / Buck, V. / Rosenfeldt, M. / Knapp, S. / Pluckthun, ...著者: Strubel, A. / Munick, P. / Hartmann, O. / Chaikuad, A. / Dreier, B. / Schaefer, J.V. / Gebel, J. / Osterburg, C. / Tuppi, M. / Schafer, B. / Buck, V. / Rosenfeldt, M. / Knapp, S. / Pluckthun, A. / Diefenbacher, M.E. / Dotsch, V. #1: ジャーナル: Chemmedchem / 年: 2023 タイトル: Structural Basis of Saccharin Derivative Inhibition of Carbonic Anhydrase IX. 著者: Leitans, J. / Kazaks, A. / Bogans, J. / Supuran, C.T. / Akopjana, I. / Ivanova, J. / Zalubovskis, R. / Tars, K. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 8p9e.cif.gz | 118.8 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb8p9e.ent.gz | 91 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 8p9e.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 8p9e_validation.pdf.gz | 438.8 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 8p9e_full_validation.pdf.gz | 439.5 KB | 表示 | |
XML形式データ | 8p9e_validation.xml.gz | 11.4 KB | 表示 | |
CIF形式データ | 8p9e_validation.cif.gz | 14.7 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/p9/8p9e ftp://data.pdbj.org/pub/pdb/validation_reports/p9/8p9e | HTTPS FTP |
-関連構造データ
関連構造データ | 8p9cC 8p9dC C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
-集合体
登録構造単位 |
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1 |
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単位格子 |
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-要素
#1: タンパク質 | 分子量: 7380.309 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: TP63, KET, P63, P73H, P73L, TP73L / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: Q9H3D4 |
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#2: タンパク質・ペプチド | 分子量: 6034.828 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: TP73, P73 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: O15350 |
#3: タンパク質 | 分子量: 16872.984 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Lama glama (ラマ) / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) |
#4: 化合物 | ChemComp-GOL / |
#5: 水 | ChemComp-HOH / |
研究の焦点であるリガンドがあるか | N |
-実験情報
-実験
実験 | 手法: X線回折 / 使用した結晶の数: 1 |
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-試料調製
結晶 | マシュー密度: 2.25 Å3/Da / 溶媒含有率: 45.25 % |
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結晶化 | 温度: 293.15 K / 手法: 蒸気拡散法, シッティングドロップ法 / pH: 6.5 詳細: 0.2M sodium chloride, 25% PEG3350, 0.1M bis-tris pH 6.5 |
-データ収集
回折 | 平均測定温度: 100 K / Serial crystal experiment: N |
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放射光源 | 由来: シンクロトロン / サイト: SLS / ビームライン: X06SA / 波長: 1 Å |
検出器 | タイプ: DECTRIS EIGER X 16M / 検出器: PIXEL / 日付: 2019年5月16日 |
放射 | プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray |
放射波長 | 波長: 1 Å / 相対比: 1 |
反射 | 解像度: 2.25→49.05 Å / Num. obs: 12746 / % possible obs: 99.4 % / 冗長度: 7 % / CC1/2: 0.998 / Rmerge(I) obs: 0.099 / Rpim(I) all: 0.042 / Rrim(I) all: 0.113 / Χ2: 0.87 / Net I/σ(I): 8.6 |
反射 シェル | 解像度: 2.25→2.33 Å / 冗長度: 6.8 % / Rmerge(I) obs: 0.777 / Mean I/σ(I) obs: 2.1 / Num. unique obs: 1208 / CC1/2: 0.883 / Rpim(I) all: 0.344 / Rrim(I) all: 0.905 / Χ2: 0.86 |
-解析
ソフトウェア |
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精密化 | 構造決定の手法: 分子置換 / 解像度: 2.25→49.05 Å / Cor.coef. Fo:Fc: 0.966 / Cor.coef. Fo:Fc free: 0.926 / SU B: 20.913 / SU ML: 0.225 / 交差検証法: THROUGHOUT / ESU R: 0.328 / ESU R Free: 0.239 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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溶媒の処理 | イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.2 Å / 溶媒モデル: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
原子変位パラメータ | Biso mean: 61.647 Å2
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精密化ステップ | サイクル: 1 / 解像度: 2.25→49.05 Å
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拘束条件 |
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