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- PDB-8ovt: Crystal structure of the ligand-free YeGT glycosyltransferase -

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Basic information

Entry
Database: PDB / ID: 8ovt
TitleCrystal structure of the ligand-free YeGT glycosyltransferase
ComponentsYeGT glycosyltransferase
KeywordsTRANSFERASE / Glycosyltransferase / effector protein / nucleotide binding protein
Biological speciesYersinia enterocolitica (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å
AuthorsWirth, C. / Hunte, C.
Funding support Germany, 2items
OrganizationGrant numberCountry
German Research Foundation (DFG)CRC 746 Germany
German Federal Ministry for Education and ResearchEXC-2189 Germany
CitationJournal: J.Biol.Chem. / Year: 2024
Title: Tyrosine-modifying glycosylation by Yersinia effectors.
Authors: Schneider, S. / Wirth, C. / Jank, T. / Hunte, C. / Aktories, K.
History
DepositionApr 26, 2023Deposition site: PDBE / Processing site: PDBE
Revision 1.0May 15, 2024Provider: repository / Type: Initial release
Revision 1.1Jun 5, 2024Group: Database references / Category: citation / Item: _citation.journal_volume

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: YeGT glycosyltransferase
B: YeGT glycosyltransferase
C: YeGT glycosyltransferase
D: YeGT glycosyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)136,41215
Polymers135,8364
Non-polymers57611
Water3,963220
1
A: YeGT glycosyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)34,0563
Polymers33,9591
Non-polymers982
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: YeGT glycosyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)34,1184
Polymers33,9591
Non-polymers1603
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: YeGT glycosyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)34,1184
Polymers33,9591
Non-polymers1603
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: YeGT glycosyltransferase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)34,1184
Polymers33,9591
Non-polymers1603
Water181
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)156.854, 156.854, 154.964
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number96
Space group name H-MP43212
Space group name HallP4nw2abw
Symmetry operation#1: x,y,z
#2: -y+1/2,x+1/2,z+3/4
#3: y+1/2,-x+1/2,z+1/4
#4: x+1/2,-y+1/2,-z+1/4
#5: -x+1/2,y+1/2,-z+3/4
#6: -x,-y,z+1/2
#7: y,x,-z
#8: -y,-x,-z+1/2

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Components

#1: Protein
YeGT glycosyltransferase


Mass: 33958.883 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Yersinia enterocolitica (bacteria) / Strain: Y2wildboar1B / Plasmid: pET28a / Production host: Escherichia coli BL21(DE3) (bacteria)
#2: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: C2H6O2
#3: Chemical
ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Cl
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 220 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.58 Å3/Da / Density % sol: 65.64 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: 10 mM Tri-sodium citrate, 16% PEG 6000.

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ESRF / Beamline: ID23-1 / Wavelength: 1 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 5, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.3→49.1 Å / Num. obs: 57009 / % possible obs: 96.4 % / Redundancy: 38.6 % / CC1/2: 1 / Rmerge(I) obs: 0.188 / Rpim(I) all: 0.045 / Net I/σ(I): 17.1
Reflection shellResolution: 2.3→2.52 Å / Rmerge(I) obs: 2.598 / Mean I/σ(I) obs: 1.8 / Num. unique obs: 2860 / CC1/2: 0.85 / Rpim(I) all: 0.58

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Processing

Software
NameVersionClassification
PHENIX1.18.2_3874refinement
autoPROCdata processing
STARANISOdata scaling
PHASERphasing
MxCuBEdata collection
Cootmodel building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.3→49.06 Å / SU ML: 0.3004 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 32.8514
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.245 2998 5.26 %
Rwork0.2042 53976 -
obs0.2063 56974 66.25 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 61.97 Å2
Refinement stepCycle: LAST / Resolution: 2.3→49.06 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms9177 0 32 220 9429
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00179412
X-RAY DIFFRACTIONf_angle_d0.419112680
X-RAY DIFFRACTIONf_chiral_restr0.03971352
X-RAY DIFFRACTIONf_plane_restr0.00351638
X-RAY DIFFRACTIONf_dihedral_angle_d18.25833607
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.3-2.340.2659150.2812360X-RAY DIFFRACTION9.26
2.34-2.380.2996270.2949430X-RAY DIFFRACTION11.42
2.38-2.420.3063290.3098508X-RAY DIFFRACTION13.32
2.42-2.470.3958250.2852593X-RAY DIFFRACTION15.31
2.47-2.520.2443450.2982790X-RAY DIFFRACTION20.75
2.52-2.570.3234750.31231134X-RAY DIFFRACTION29.82
2.57-2.630.2737640.29131366X-RAY DIFFRACTION35.52
2.63-2.70.3332750.30331582X-RAY DIFFRACTION40.82
2.7-2.770.34381180.29841953X-RAY DIFFRACTION51.11
2.77-2.850.351610.30922552X-RAY DIFFRACTION66.64
2.85-2.950.32842070.30543460X-RAY DIFFRACTION90.45
2.95-3.050.33632440.29213794X-RAY DIFFRACTION99.04
3.05-3.170.31151760.27533882X-RAY DIFFRACTION99.98
3.17-3.320.31851940.24743884X-RAY DIFFRACTION100
3.32-3.490.2742210.22723894X-RAY DIFFRACTION100
3.49-3.710.2422290.20793867X-RAY DIFFRACTION99.98
3.71-40.22922550.17853873X-RAY DIFFRACTION99.98
4-4.40.19972160.14993911X-RAY DIFFRACTION100
4.4-5.030.19772110.14623958X-RAY DIFFRACTION100
5.03-6.340.23421950.18744011X-RAY DIFFRACTION99.98
6.34-49.060.18392160.17024174X-RAY DIFFRACTION99.43
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL112)L122)L132)L222)L232)L332)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T112)T122)T132)T222)T232)T332)Origin x (Å)Origin y (Å)Origin z (Å)
18.87742893924-5.572607542435.394691066847.55188558834-0.4427184292935.595739510950.397781287139-0.1086427178131.495151029060.006596684612690.185531823737-0.513181585055-0.999837717165-0.113506069273-0.5309385522030.824568854798-0.1587994024170.3791115020050.6439112866270.04315935478850.758870153289-34.2910598551-9.10033747285-23.7343409889
21.8663774718-0.3944624259380.5069846382790.577546092398-0.4634779107181.389014769880.384230132861-0.7236819878490.2095216995820.735333701713-0.154788843841-0.327205075701-0.02731796676850.06763929848960.3473883724430.73620873051-0.585069750061-0.2925231198061.052227559940.1920542766710.299643996421-5.21173680322-36.1172593683-5.72303373877
33.15104253629-0.3181848698291.296016918044.064354734781.027085331842.597869076920.254055394013-0.002611319014910.1497168963830.212543918176-0.3538123782290.217479296196-0.1989289161090.4399266238380.03350317252830.467304868379-0.3311859509570.01259373115440.7974716296810.04390954095220.247695967113-18.9330560389-32.5514093347-15.8021519519
42.1816431724-2.41191480916-0.0509644803172.89086966682-0.4247638710671.039675653240.459687307086-0.395796100247-0.5412926049280.578779175353-0.1205365568210.9074906887710.257549310222-0.8693281504370.555872589290.977075449789-0.6985718336860.04449272878211.176212497810.05659128641040.446769444357-24.0922539184-41.930411697-4.62306474691
55.36646317459-2.949839821072.569083331052.32477382366-1.679740395372.287758693950.572813383928-0.05366453431860.2664139451740.176024253362-0.3538472573210.30380060092-0.2050304811320.377879844808-0.2325904465990.424904116151-0.1686307783550.1983721006810.428656143183-0.02828014769340.500342165556-34.0538015428-23.2788455185-26.6956338203
64.00328913111-1.33691941538-1.211777724641.877095374732.407821534773.166694238280.3082271065920.37403935521-0.1513710768080.741005969407-0.474913349469-0.1929450713680.245740668860.6795402774430.08686367524340.555327454609-0.321948845358-0.007036799068790.9173540836580.08162957726780.237769230678-14.3455931203-31.2376314979-20.7225417278
71.72026025157-1.32107111584-1.170291782473.152030841651.493216727212.983109661660.02019349014030.0188899086722-0.336201277636-0.1079117721280.04058173027630.07908344604490.255437155691-0.0681901521297-0.06808222652490.280293193689-0.03577537306450.004162856801950.1950783989350.04178189898730.214283190257-46.7155138827-69.67579785178.66950959712
81.11332693576-0.858652989178-0.8158828383561.281229940511.683267755713.174786883960.414431131043-0.02994337800450.423662467301-0.6061344847610.211723022975-0.450064069346-0.5867160844780.134220653789-0.5335849780210.52485117748-0.03701466000840.1960894773140.2783227530770.04378484767220.33031495848-47.3512784446-50.9194444613.6590616507
91.53954307559-2.75509301822-1.464809658026.775924862622.778996400753.47660740782-0.144272858531-0.112775625081-0.06817344949870.4145483077650.2049880963840.1195058623430.0998426921951-0.166044967457-0.03133340289810.303512710112-0.0434316669150.1040128547380.3123714784940.1070345491040.263244856925-58.2156124323-52.810987073927.6463657844
102.127343721021.44004966348-0.8170925072040.951444041145-0.7531668079611.664148518870.209296941623-0.0575939030939-0.406845135683-0.142111290035-0.455996393727-0.6306816986190.06494104037560.7326767295620.1823400600750.2790396651930.1306297422830.01856697451640.91359189190.01024254079480.498923678817-7.8020210766-43.3771761001-38.0788896808
112.939698548431.45398140063-1.351992929984.36036671192-1.625908589552.631555719050.2050088746320.4606296234540.252991718652-0.4843490645650.00749956787008-0.261559140798-0.1293619943880.122059737963-0.2002952570770.4415141551910.02370215100890.1436474787680.8905628362430.06772164557760.321361402371-8.66094886511-29.1944323756-52.3755104497
123.88102956921-0.973504267291-0.570057678583.45164169761.244716364485.267322133360.258601266298-0.0654749583737-0.4287394215310.0349485580931-0.253497910138-0.35828972685-0.4333891765970.901486584444-0.03940916112150.3057949148180.04530995819170.0203533386181.004214948270.1483914068370.482099371934-4.75297003925-38.2594809348-36.5247570422
133.062203725860.678814992247-2.297382425436.086013325250.6242847594223.492947981810.2774899753130.0001173878785010.0353993047187-0.207372019097-0.0378771657964-0.48011708994-0.04768934641650.0938508631998-0.1964467534840.2073691026050.03548773844550.002709691304620.8007349449940.008195435501220.242217640971-17.5235421518-40.7673581039-35.9909372504
141.337841273170.631155073175-0.06433966386167.45109765397-4.095849892513.864453830850.2063343138760.6917332631530.218037157907-0.2886774868390.2521861470670.756278425696-0.172886920731-0.537337910852-0.4504277964950.2446483201830.0829637959571-0.003656037206060.7512867076570.01738977164950.328922069448-25.4041486478-37.6254541431-43.8526304983
153.386460409472.647182100560.3467856514934.54332638223-1.018349772791.203910441340.883029803763-0.616794913732-0.0413937830381.00607635488-0.736493212802-0.0605620258547-0.03003710873340.559993263927-0.1351188704890.353662125317-0.0478948838086-0.07534871313220.543274314924-0.05329878851580.279100605626-31.495162555-59.0383296272-19.743014176
164.945661411993.98335568091-3.067452330836.23309258694-2.271106394951.916485583040.7031470296410.126523057289-0.887048468980.340483045413-1.25201152129-0.561606128557-0.0888415533051.006774942560.4968537831270.2540877050230.0605608110994-0.09131059351580.7850714491790.02456442039520.545804079906-18.5834555448-55.8034494106-26.1885438813
172.503749441381.0729831664-0.4892111432556.921884212295.285833265314.838380499750.550012858959-0.5282852298350.36252199595-0.481838424302-0.535085735231-0.412868101574-0.6325990166821.13278917523-0.1453716981540.458435833415-0.1014427788760.09362099169641.059744548630.1264353460230.444591790547-10.5790529311-34.3784161096-31.396791924
183.773555907132.961337029221.147808839613.865368967642.430759294362.531715915560.197800796870.0392415706320.801086886119-0.373456945564-0.2361633160040.541945621669-0.398813089419-0.1083961387910.03790314294940.3640277605090.07456165520020.03064010184950.4373424558570.1831053450390.540780829205-66.462232388-32.6216828115-37.986260321
194.08178386688-0.769230040859-0.7229446961852.063447677111.521706851863.967814742770.229650849440.417805147757-0.271043878185-0.104023638208-0.4241624643320.6883310418040.83113036452-1.192761463580.1411501302730.458339365866-0.157005496048-0.2227550156790.7337211761070.06574105897130.491099797986-80.5885917539-54.6529730347-43.0516784471
202.885446885691.662725219382.054885740993.505608479310.2497356177053.451278780280.09916573410870.8795634546160.0321017741743-0.6741963351650.2437089826550.1575496531020.149767953909-0.00870509868805-0.2188527160470.4326806796710.05229603867-0.1472759358420.709862046247-0.03909396833970.274233171571-69.1288011922-51.9547254384-52.1562869672
213.547486620061.336983324470.22277973473.589307246460.572713733834.225403934550.243319455584-0.02180680739030.457299925349-0.1092050258110.01674252911490.09813442514610.0489584497425-0.172503938695-0.1976566265370.1898633843880.0593935174825-0.04044194967880.3132300726360.03135012783440.273793320951-66.9545547148-42.2604156273-36.2909442872
226.56224174208-2.004063715291.849937452747.799389742690.6066617433794.464668182780.1911472495910.781977516923-0.523205748640.09331648435310.114195342874-1.179419588140.4730903408711.10267938502-0.2454855000580.3320305788630.111753647985-0.04961837934890.6351653095390.01467504339120.418301150446-54.1165692204-48.7571712569-47.1236231372
232.947678567282.642849478910.8919556477134.881164293051.913841608932.708719761810.563695734248-0.1473123032420.4906429472970.0563779185457-0.034766506242-0.0850761877819-0.3633553872710.227316933487-0.4328366234240.282759916385-0.08869419354090.2118022980750.4629181199140.02405339134110.534080477847-50.6574209997-27.4131913409-26.5963225531
243.217536940772.395631386792.991501417226.169589299536.843822628628.895430374980.711713752337-0.6135325749670.904600636062-0.00754831715768-0.8417622220370.876602800377-0.369596737341-0.8600657314770.1604394548960.364209253926-0.0136077096550.152825405830.3772355028270.04744044843570.817683155004-61.3582387968-25.7249250817-27.577159763
252.409014366950.935160949463-0.7542140973327.38769783662-7.097525306728.853105620990.483239058377-0.601916522248-0.376288947867-0.4106419250770.307292871231-0.2198845820050.555647975591-0.626433950361-0.5870490227110.336710355425-0.074364187832-0.2324260749840.5252016961260.07543732621370.443289396121-69.0395942985-47.3728116628-31.0240162995
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 15 through 28 )AA15 - 281 - 14
22chain 'A' and (resid 29 through 162 )AA29 - 16215 - 148
33chain 'A' and (resid 163 through 195 )AA163 - 195149 - 181
44chain 'A' and (resid 196 through 221 )AA196 - 221182 - 207
55chain 'A' and (resid 222 through 279 )AA222 - 279208 - 265
66chain 'A' and (resid 280 through 301 )AA280 - 301266 - 287
77chain 'B' and (resid 16 through 195 )BB16 - 1951 - 181
88chain 'B' and (resid 196 through 255 )BB196 - 255182 - 241
99chain 'B' and (resid 256 through 299 )BB256 - 299242 - 285
1010chain 'C' and (resid 16 through 74 )CC16 - 741 - 59
1111chain 'C' and (resid 75 through 144 )CC75 - 14460 - 129
1212chain 'C' and (resid 145 through 162 )CC145 - 162130 - 147
1313chain 'C' and (resid 163 through 195 )CC163 - 195148 - 180
1414chain 'C' and (resid 196 through 237 )CC196 - 237181 - 222
1515chain 'C' and (resid 238 through 272 )CC238 - 272223 - 257
1616chain 'C' and (resid 273 through 286 )CC273 - 286258 - 271
1717chain 'C' and (resid 287 through 301 )CC287 - 301272 - 286
1818chain 'D' and (resid 16 through 51 )DD16 - 511 - 36
1919chain 'D' and (resid 52 through 91 )DD52 - 9137 - 76
2020chain 'D' and (resid 92 through 144 )DD92 - 14477 - 129
2121chain 'D' and (resid 145 through 195 )DD145 - 195130 - 180
2222chain 'D' and (resid 196 through 221 )DD196 - 221181 - 206
2323chain 'D' and (resid 222 through 272 )DD222 - 272207 - 257
2424chain 'D' and (resid 273 through 286 )DD273 - 286258 - 271
2525chain 'D' and (resid 287 through 301 )DD287 - 301272 - 286

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