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Open data
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Basic information
Entry | Database: PDB / ID: 8ovs | |||||||||
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Title | Crystal structure of YeGT glycosyltransferase with bound UDP | |||||||||
![]() | YeGT glycosyltransferase | |||||||||
![]() | TRANSFERASE / Glycosyltransferase / effector protein / nucleotide binding protein | |||||||||
Function / homology | ACETATE ION / : / : / URIDINE-5'-DIPHOSPHATE![]() | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Wirth, C. / Hunte, C. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Tyrosine-modifying glycosylation by Yersinia effectors. Authors: Schneider, S. / Wirth, C. / Jank, T. / Hunte, C. / Aktories, K. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 271.2 KB | Display | ![]() |
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PDB format | ![]() | Display | ![]() | |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3 MB | Display | ![]() |
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Full document | ![]() | 3 MB | Display | |
Data in XML | ![]() | 31.6 KB | Display | |
Data in CIF | ![]() | 50 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8ovtC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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2 |
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AAABBB
#1: Protein | Mass: 33958.883 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 7 types, 910 molecules ![](data/chem/img/UDP.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/CD.gif)
![](data/chem/img/MN.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/EDO.gif)
![](data/chem/img/CD.gif)
![](data/chem/img/MN.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | #3: Chemical | ChemComp-ACT / #4: Chemical | ChemComp-EDO / #5: Chemical | ChemComp-CD / #6: Chemical | #7: Chemical | #8: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.76 Å3/Da / Density % sol: 55.39 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 100mM HEPES pH 7.5, 500mM sodium acetate, 50mM cadmium sulfate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 13, 2017 | ||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | ||||||||||||||||||
Reflection twin |
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Reflection | Resolution: 1.12→47.13 Å / Num. obs: 213705 / % possible obs: 92.7 % / Redundancy: 30.3 % / CC1/2: 0.999 / Rmerge(I) obs: 0.132 / Rpim(I) all: 0.023 / Net I/σ(I): 18 | ||||||||||||||||||
Reflection shell | Resolution: 1.12→1.22 Å / Redundancy: 18.4 % / Rmerge(I) obs: 2.038 / Mean I/σ(I) obs: 1.7 / Num. unique obs: 10686 / CC1/2: 0.39 / Rpim(I) all: 0.479 |
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Processing
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Refinement | Method to determine structure: ![]() Details: Hydrogens have been added in their riding positions during refinement. Twin refinement was used with a twin fraction of 0.466 for -h, -k, l as determined by Refmac.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 17.643 Å2
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Refinement step | Cycle: LAST / Resolution: 1.123→47.13 Å
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Refine LS restraints |
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LS refinement shell |
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