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Yorodumi- PDB-8ka0: Crystal structure of Vibrio vulnificus RID-dependent transforming... -
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-Basic information
Entry | Database: PDB / ID: 8ka0 | |||||||||
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Title | Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-bound calmodulin and a nicotinamide adenine dinucleotide (NAD+) | |||||||||
Components |
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Keywords | TOXIN / MARTX toxin / RDTND-RID / NADase / CaM / NAD+ | |||||||||
Function / homology | Function and homology information Ligases; Forming carbon-nitrogen bonds; Acid-amino-acid ligases (peptide synthases) / negative regulation of calcium ion transmembrane transporter activity / host cell cytosol / positive regulation of cyclic-nucleotide phosphodiesterase activity / negative regulation of calcium ion export across plasma membrane / acyltransferase activity / positive regulation of ryanodine-sensitive calcium-release channel activity / regulation of cell communication by electrical coupling involved in cardiac conduction / negative regulation of peptidyl-threonine phosphorylation / ligase activity ...Ligases; Forming carbon-nitrogen bonds; Acid-amino-acid ligases (peptide synthases) / negative regulation of calcium ion transmembrane transporter activity / host cell cytosol / positive regulation of cyclic-nucleotide phosphodiesterase activity / negative regulation of calcium ion export across plasma membrane / acyltransferase activity / positive regulation of ryanodine-sensitive calcium-release channel activity / regulation of cell communication by electrical coupling involved in cardiac conduction / negative regulation of peptidyl-threonine phosphorylation / ligase activity / protein phosphatase activator activity / positive regulation of phosphoprotein phosphatase activity / adenylate cyclase binding / catalytic complex / detection of calcium ion / regulation of cardiac muscle contraction / negative regulation of ryanodine-sensitive calcium-release channel activity / calcium channel inhibitor activity / regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion / regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum / cysteine-type peptidase activity / positive regulation of protein dephosphorylation / regulation of calcium-mediated signaling / titin binding / positive regulation of protein autophosphorylation / voltage-gated potassium channel complex / sperm midpiece / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups / calcium channel complex / substantia nigra development / adenylate cyclase activator activity / regulation of heart rate / sarcomere / protein serine/threonine kinase activator activity / regulation of cytokinesis / positive regulation of peptidyl-threonine phosphorylation / spindle microtubule / positive regulation of protein serine/threonine kinase activity / spindle pole / response to calcium ion / calcium-dependent protein binding / G2/M transition of mitotic cell cycle / myelin sheath / toxin activity / vesicle / transmembrane transporter binding / Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases / G protein-coupled receptor signaling pathway / centrosome / lipid binding / calcium ion binding / protein kinase binding / host cell plasma membrane / protein-containing complex / proteolysis / extracellular region / membrane / nucleus / metal ion binding / plasma membrane / cytoplasm Similarity search - Function | |||||||||
Biological species | Vibrio vulnificus (bacteria) Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.35 Å | |||||||||
Authors | Lee, Y. / Choi, S. / Hwang, J. / Kim, M.H. | |||||||||
Funding support | Korea, Republic Of, 2items
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Citation | Journal: To Be Published Title: Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-bound calmodulin and ...Title: Crystal structure of Vibrio vulnificus RID-dependent transforming NADase domain (RDTND)/calmodulin-binding domain of Rho inactivation domain (RID-CBD) complexed with Ca2+-bound calmodulin and a nicotinamide adenine dinucleotide (NAD+) Authors: Lee, Y. / Choi, S. / Hwang, J. / Kim, M.H. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8ka0.cif.gz | 458.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8ka0.ent.gz | 374.4 KB | Display | PDB format |
PDBx/mmJSON format | 8ka0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8ka0_validation.pdf.gz | 5.2 MB | Display | wwPDB validaton report |
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Full document | 8ka0_full_validation.pdf.gz | 5.2 MB | Display | |
Data in XML | 8ka0_validation.xml.gz | 87.2 KB | Display | |
Data in CIF | 8ka0_validation.cif.gz | 119.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ka/8ka0 ftp://data.pdbj.org/pub/pdb/validation_reports/ka/8ka0 | HTTPS FTP |
-Related structure data
Related structure data | 8k9zS S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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3 |
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4 |
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Unit cell |
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-Components
-Protein , 2 types, 8 molecules ACEGBDFH
#1: Protein | Mass: 47092.160 Da / Num. of mol.: 4 / Fragment: DUF1-RIDcbd (residues, 1959-2374) / Mutation: E2186Q Source method: isolated from a genetically manipulated source Details: NCBI accession ID: WP_015728045.1 / Source: (gene. exp.) Vibrio vulnificus (bacteria) / Gene: CRN52_02910 / Production host: Escherichia coli (E. coli) References: UniProt: A0A2S3R7M0, Hydrolases; Acting on peptide bonds (peptidases); Cysteine endopeptidases #2: Protein | Mass: 16979.691 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: N-terminal additional vector sequences, GA / Source: (gene. exp.) Homo sapiens (human) / Gene: CALM2, CAM2, CAMB / Production host: Escherichia coli (E. coli) / References: UniProt: P0DP24 |
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-Non-polymers , 4 types, 632 molecules
#3: Chemical | ChemComp-NAD / #4: Chemical | ChemComp-CA / #5: Chemical | ChemComp-GOL / #6: Water | ChemComp-HOH / | |
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-Details
Has ligand of interest | Y |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.38 Å3/Da / Density % sol: 48.25 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.1 M Tris-HCl (pH 8.5), 32.5% (w/v) PEG 4000 and 0.15 M sodium acetate. 10 mM NAD+ soaking |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: PAL/PLS / Beamline: 5C (4A) / Wavelength: 1 Å |
Detector | Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Mar 24, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→29.62 Å / Num. obs: 98734 / % possible obs: 99.8 % / Redundancy: 6.9 % / CC1/2: 0.999 / Net I/σ(I): 11.6 |
Reflection shell | Resolution: 2.35→2.39 Å / Num. unique obs: 4831 / CC1/2: 0.675 / % possible all: 99.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 8K9Z Resolution: 2.35→29.62 Å / Cor.coef. Fo:Fc: 0.952 / Cor.coef. Fo:Fc free: 0.924 / Cross valid method: THROUGHOUT / ESU R: 0.494 / ESU R Free: 0.279 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 55.8 Å2
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Refinement step | Cycle: LAST / Resolution: 2.35→29.62 Å
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