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Open data
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Basic information
Entry | Database: PDB / ID: 8io3 | ||||||
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Title | Cryo-EM structure of human HCN3 channel with cilobradine | ||||||
![]() | Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 | ||||||
![]() | MEMBRANE PROTEIN / human HCN3 channel | ||||||
Function / homology | ![]() cone cell pedicle / HCN channels / HCN channel complex / regulation of membrane depolarization / cellular response to dopamine / voltage-gated sodium channel activity / voltage-gated potassium channel activity / sodium ion transmembrane transport / cAMP binding / potassium ion transmembrane transport ...cone cell pedicle / HCN channels / HCN channel complex / regulation of membrane depolarization / cellular response to dopamine / voltage-gated sodium channel activity / voltage-gated potassium channel activity / sodium ion transmembrane transport / cAMP binding / potassium ion transmembrane transport / regulation of membrane potential / axon / neuronal cell body / dendrite / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.02 Å | ||||||
![]() | Yu, B. / Lu, Q.Y. / Li, J. / Zhang, J. | ||||||
Funding support | 1items
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![]() | ![]() Title: Cryo-EM structure of human HCN3 channel with cilobradine Authors: Yu, B. / Lu, Q.Y. / Li, J. / Zhang, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 323.6 KB | Display | ![]() |
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PDB format | ![]() | 252.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 35606MC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 86142.922 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: Mammalian expression vector EGFP-MCS-pcDNA3.1 (others) References: UniProt: Q9P1Z3 #2: Chemical | ChemComp-VXI / Mass: 482.612 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C28H38N2O5 / Feature type: SUBJECT OF INVESTIGATION Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: otassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3 Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: Mammalian expression vector BsrGI-MCS-pcDNA3.1 (others) |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2200 nm / Nominal defocus min: 1800 nm |
Image recording | Electron dose: 40 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 3.02 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 121387 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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