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Open data
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Basic information
Entry | Database: PDB / ID: 8ik7 | ||||||
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Title | Cryo-EM structure of hnRAC1 fibril. | ||||||
![]() | GLY-PHE-GLY-GLY-ASN-ASP-ASN-PHE-GLY | ||||||
![]() | RNA BINDING PROTEIN / reversible fibril | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.69 Å | ||||||
![]() | Li, D.N. / Ma, Y.Y. / Li, D. / Dai, B. / Liu, C. | ||||||
Funding support | 1items
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![]() | ![]() Title: Formed fibrils of hnRNP A1 reversible amyloid core GFGGNDNFG (residues 209-217) Authors: Li, D.N. / Ma, Y.Y. / Li, D. / Dai, B. / Liu, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 38.2 KB | Display | ![]() |
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PDB format | ![]() | 29.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 30.4 KB | Display | |
Data in CIF | ![]() | 41.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein/peptide | Mass: 883.863 Da / Num. of mol.: 24 / Source method: obtained synthetically / Source: (synth.) ![]() |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: helical reconstruction |
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Sample preparation
Component | Name: Formed fibrils of hnRNP A1 reversible amyloid core GFGGNDNFG (residues 209-217) Type: ORGANELLE OR CELLULAR COMPONENT / Entity ID: all / Source: RECOMBINANT |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() |
Buffer solution | pH: 7 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 55 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
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Processing
CTF correction | Type: NONE | ||||||||||||||||||||||||
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Helical symmerty | Angular rotation/subunit: -1.09 ° / Axial rise/subunit: 4.81 Å / Axial symmetry: C1 | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.69 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 16505 / Symmetry type: HELICAL | ||||||||||||||||||||||||
Refine LS restraints |
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