+Open data
-Basic information
Entry | Database: PDB / ID: 8hcy | ||||||
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Title | Legionella glycosyltransferase | ||||||
Components | Dot/Icm secretion system substrate | ||||||
Keywords | TRANSFERASE / glucosyltransferase / upd-glucose | ||||||
Function / homology | Glycosyltransferase family 88 / Glycosyltransferase family 88 / Dot/Icm secretion system substrate Function and homology information | ||||||
Biological species | Legionella (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.64 Å | ||||||
Authors | Chen, T.T. / Ouyang, S.Y. | ||||||
Funding support | China, 1items
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Citation | Journal: Small Struct / Year: 2023 Title: Structural Basis for the Action Mechanism of Legionella Glycosyltransferase. Authors: Chen, T.T. / Zheng, S.R. / Tian, L. / Lv, S.L. / Zhong, W.H. / Li, X.L. / Zhang, D.D. / Zheng, X.X. / Ouyang, S.Y. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8hcy.cif.gz | 836.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8hcy.ent.gz | 648 KB | Display | PDB format |
PDBx/mmJSON format | 8hcy.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8hcy_validation.pdf.gz | 482.5 KB | Display | wwPDB validaton report |
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Full document | 8hcy_full_validation.pdf.gz | 513.9 KB | Display | |
Data in XML | 8hcy_validation.xml.gz | 71.8 KB | Display | |
Data in CIF | 8hcy_validation.cif.gz | 99.4 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hc/8hcy ftp://data.pdbj.org/pub/pdb/validation_reports/hc/8hcy | HTTPS FTP |
-Related structure data
Related structure data | 7xgvSC 7xgxC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 98615.453 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Legionella (bacteria) / Gene: C3927_06670 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A2S6F8W2 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.58 Å3/Da / Density % sol: 21.96 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, sitting drop / Details: PEG |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17B1 / Wavelength: 0.9792 Å |
Detector | Type: DECTRIS PILATUS 200K / Detector: PIXEL / Date: Jul 14, 2021 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9792 Å / Relative weight: 1 |
Reflection | Resolution: 2.64→29.31 Å / Num. obs: 71942 / % possible obs: 95.51 % / Redundancy: 6.7 % / Biso Wilson estimate: 28.23 Å2 / CC1/2: 0.971 / Rmerge(I) obs: 0.13 / Net I/σ(I): 4.07 |
Reflection shell | Resolution: 2.64→2.73 Å / Num. unique obs: 68773 / CC1/2: 0.314 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 7XGV Resolution: 2.64→29.31 Å / SU ML: 0.2921 / Cross valid method: FREE R-VALUE / σ(F): 0.15 / Phase error: 27.563 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.66 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.64→29.31 Å
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Refine LS restraints |
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LS refinement shell |
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