Mass: 14472.172 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Trichonephila clavipes (spider) / Gene: MaSp2 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A2S2B4G5
-
Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
isotropic
1
2D 1H-15N HSQC; 2D 1H-13C HSQC ALIPHATIC; 2D 1H-13C HSQC AROMATIC; 3D CBCA(CO)NH; 3D HN(CA)CB; 3D HNCO; 3D HN(CA)CO; 3D HNHAHB; 3D HBHA(CO)NH; 3D 1H-13C NOESY ALIPHATIC; 3D 1H-15N NOESY; 3D H(CCO)NH; 3D (H)CCH-TOCSY; 2D HB(CBCG)CD(HD); 2D HB(CBCGCD) CE(HE)
1
2
1
isotropic
1
2D 1H-13C HSQC aliphatic
1
3
1
isotropic
1
2D 1H-13C HSQC aromatic
1
4
1
isotropic
1
3DCBCA(CO)NH
1
5
1
isotropic
1
3D HN(CA)CB
1
8
1
isotropic
1
3D HNCO
1
7
1
isotropic
1
3DHN(CA)CO
1
6
1
isotropic
1
3DHBHA(CO)NH
1
9
1
isotropic
1
3DHNHAHB
1
10
1
isotropic
1
3D 1H-13C NOESY aliphatic
1
11
1
isotropic
1
3D 1H-15N NOESY
1
12
1
isotropic
1
3DH(CCO)NH
1
13
1
isotropic
1
3D (H)CCH-TOCSY
1
14
1
isotropic
1
2DHB(CBCGCD)CE(HE)
1
15
1
isotropic
1
2DHB(CBCG)CD(HD)
NMR details
Text: NULL
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Sample preparation
Details
Type: fibrous protein Contents: 1 mM [U-13C; U-15N] N-terminal domain of NTD MaSp2 from Triconephila clavipes, 0.1 mM DSS, 10 % v/v [U-100% 2H] D2O, 300 mM sodium chloride, 10 mM sodium phosphate, 90% H2O/10% D2O Label: 13C_15N NTD MaSp2 / Solvent system: 90% H2O/10% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
1mM
N-terminal domain of NTD MaSp2 from Triconephila clavipes
[U-13C; U-15N]
1
0.1mM
DSS
naturalabundance
1
10 % v/v
D2O
[U-100% 2H]
1
300mM
sodiumchloride
naturalabundance
1
10mM
sodiumphosphate
naturalabundance
1
Sample conditions
Ionic strength: 300 mM / Label: conditions_1 / pH: 7 / Pressure: 1 atm / Temperature: 298 K
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