National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
75N93022C00036
米国
引用
ジャーナル: Structure / 年: 2024 タイトル: Structural basis for the broad antigenicity of the computationally optimized influenza hemagglutinin X6. 著者: Kaito A Nagashima / John V Dzimianski / Meng Yang / Jan Abendroth / Giuseppe A Sautto / Ted M Ross / Rebecca M DuBois / Thomas E Edwards / Jarrod J Mousa / 要旨: Influenza causes significant morbidity and mortality. As an alternative approach to current seasonal vaccines, the computationally optimized broadly reactive antigen (COBRA) platform has been ...Influenza causes significant morbidity and mortality. As an alternative approach to current seasonal vaccines, the computationally optimized broadly reactive antigen (COBRA) platform has been previously applied to hemagglutinin (HA). This approach integrates wild-type HA sequences into a single immunogen to expand the breadth of accessible antibody epitopes. Adding to previous studies of H1, H3, and H5 COBRA HAs, we define the structural features of another H1 subtype COBRA, X6, that incorporates HA sequences from before and after the 2009 H1N1 influenza pandemic. We determined structures of this antigen alone and in complex with COBRA-specific as well as broadly reactive and functional antibodies, analyzing its antigenicity. We found that X6 possesses features representing both historic and recent H1 HA strains, enabling binding to both head- and stem-reactive antibodies. Overall, these data confirm the integrity of broadly reactive antibody epitopes of X6 and contribute to design efforts for a next-generation vaccine.
#256 - 2021年4月 SARSコロナウイルス2型のスパイクと抗体 (SARS-CoV-2 Spike and Antibodies) 類似性 (1)
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集合体
登録構造単位
A: Hemagglutinin B: Hemagglutinin C: Hemagglutinin H: GS10-X6-BE4 Fab Heavy chain L: GS10-X6-BE4 Fab light chain M: GS10-X6-BE4 Fab Heavy chain N: GS10-X6-BE4 Fab light chain ヘテロ分子
The full sequences of the Fab chains were not provided by the depositors; only the sequences of the ...The full sequences of the Fab chains were not provided by the depositors; only the sequences of the variable domain portions are presented.
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実験情報
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実験
実験
手法: 電子顕微鏡法
EM実験
試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法
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試料調製
構成要素
名称: CryoEM structure of Influenza A virus A/Melbourner/1/1946 (H1N1) hemagglutinin bound to GS10-X6-BE4 Fab タイプ: COMPLEX / Entity ID: #1-#3 / 由来: MULTIPLE SOURCES
分子量
値: 0.221 MDa / 実験値: NO
緩衝液
pH: 8
緩衝液成分
ID
濃度
名称
Buffer-ID
1
150mM
sodiumchloride
1
2
25mM
Tris/HCl
1
試料
濃度: 0.5 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES