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- PDB-8fub: Crystal structure of human Importin alpha 3 in complex with Hendr... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8fub | ||||||
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Title | Crystal structure of human Importin alpha 3 in complex with Hendra virus matrix protein NLS1 | ||||||
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![]() | TRANSPORT PROTEIN / Importin / nuclear transport | ||||||
Function / homology | ![]() dopamine secretion / NS1 Mediated Effects on Host Pathways / NLS-dependent protein nuclear import complex / nuclear import signal receptor activity / nuclear localization sequence binding / NLS-bearing protein import into nucleus / virion assembly / nuclear pore / virion component / ISG15 antiviral mechanism ...dopamine secretion / NS1 Mediated Effects on Host Pathways / NLS-dependent protein nuclear import complex / nuclear import signal receptor activity / nuclear localization sequence binding / NLS-bearing protein import into nucleus / virion assembly / nuclear pore / virion component / ISG15 antiviral mechanism / protein import into nucleus / gene expression / nuclear membrane / host cell cytoplasm / structural constituent of virion / host cell nucleus / nucleoplasm / nucleus / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Donnelly, C.M. / Basler, C.F. / Scott, C. / Forwood, J.K. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Henipavirus Matrix Protein Employs a Non-Classical Nuclear Localization Signal Binding Mechanism. Authors: Donnelly, C.M. / Vogel, O.A. / Edwards, M.R. / Taylor, P.E. / Roby, J.A. / Forwood, J.K. / Basler, C.F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 114 KB | Display | ![]() |
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PDB format | ![]() | 69.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 430.9 KB | Display | ![]() |
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Full document | ![]() | 435.2 KB | Display | |
Data in XML | ![]() | 15.7 KB | Display | |
Data in CIF | ![]() | 20.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8fuaC ![]() 8fucC ![]() 6bvzS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 50325.812 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Protein/peptide | Mass: 2136.585 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 50.83 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 0.1M sodium HEPES,0.72M sodium citrate, 1% dithiothreitol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER2 S 16M / Detector: PIXEL / Date: Jul 25, 2019 |
Radiation | Monochromator: M / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9537 Å / Relative weight: 1 |
Reflection | Resolution: 2.75→29.78 Å / Num. obs: 13681 / % possible obs: 99.9 % / Redundancy: 11.3 % / Biso Wilson estimate: 65.77 Å2 / CC1/2: 0.995 / Rmerge(I) obs: 0.181 / Net I/σ(I): 7.8 |
Reflection shell | Resolution: 2.75→2.9 Å / Rmerge(I) obs: 1.032 / Mean I/σ(I) obs: 2.3 / Num. unique obs: 1966 / CC1/2: 0.887 / Rpim(I) all: 0.446 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6BVZ Resolution: 2.75→29.78 Å / SU ML: 0.3291 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 30.2044 / Stereochemistry target values: CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 72.53 Å2 | ||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.75→29.78 Å
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Refine LS restraints |
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LS refinement shell |
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