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- PDB-8fua: Crystal structure of mouse Importin alpha in complex with Hendra ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8fua | ||||||
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Title | Crystal structure of mouse Importin alpha in complex with Hendra virus matrix protein NLS1 | ||||||
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![]() | TRANSPORT PROTEIN / importin / nuclear transport / viral protein | ||||||
Function / homology | ![]() Sensing of DNA Double Strand Breaks / entry of viral genome into host nucleus through nuclear pore complex via importin / positive regulation of viral life cycle / NLS-dependent protein nuclear import complex / postsynapse to nucleus signaling pathway / nuclear import signal receptor activity / nuclear localization sequence binding / NLS-bearing protein import into nucleus / virion assembly / virion component ...Sensing of DNA Double Strand Breaks / entry of viral genome into host nucleus through nuclear pore complex via importin / positive regulation of viral life cycle / NLS-dependent protein nuclear import complex / postsynapse to nucleus signaling pathway / nuclear import signal receptor activity / nuclear localization sequence binding / NLS-bearing protein import into nucleus / virion assembly / virion component / cytoplasmic stress granule / protein import into nucleus / host cell / DNA-binding transcription factor binding / host cell cytoplasm / structural constituent of virion / postsynaptic density / glutamatergic synapse / host cell nucleus / nucleoplasm / nucleus / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Donnelly, C.M. / Basler, C.F. / Scott, C. / Forwood, J.K. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Henipavirus Matrix Protein Employs a Non-Classical Nuclear Localization Signal Binding Mechanism. Authors: Donnelly, C.M. / Vogel, O.A. / Edwards, M.R. / Taylor, P.E. / Roby, J.A. / Forwood, J.K. / Basler, C.F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 211.6 KB | Display | ![]() |
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PDB format | ![]() | 134.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 439.8 KB | Display | ![]() |
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Full document | ![]() | 442.8 KB | Display | |
Data in XML | ![]() | 19 KB | Display | |
Data in CIF | ![]() | 28.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8fubC ![]() 8fucC ![]() 6bw0S S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 55268.473 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() | ||
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#2: Protein/peptide | Mass: 2136.585 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.96 Å3/Da / Density % sol: 63.69 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 0.1M sodium HEPES, 0.72M sodium citrate, 1% dithiothreitol |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Jul 25, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9537 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→24.42 Å / Num. obs: 56224 / % possible obs: 99.9 % / Redundancy: 7.2 % / Biso Wilson estimate: 35.03 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.085 / Rpim(I) all: 0.05 / Net I/σ(I): 11.9 |
Reflection shell | Resolution: 1.9→1.94 Å / Redundancy: 7.1 % / Rmerge(I) obs: 1.376 / Num. unique obs: 26486 / CC1/2: 0.602 / Rpim(I) all: 0.82 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6BW0 Resolution: 1.9→24.42 Å / SU ML: 0.2161 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 21.0206 / Stereochemistry target values: CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 43.57 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→24.42 Å
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Refine LS restraints |
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LS refinement shell |
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