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- PDB-8fs1: CamA Adenine Methyltransferase Complexed to Cognate Substrate DNA... -

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Basic information

Entry
Database: PDB / ID: 8fs1
TitleCamA Adenine Methyltransferase Complexed to Cognate Substrate DNA and Inhibitor 11a (YD905)
Components
  • (DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)- ...) x 2
  • Site-specific DNA-methyltransferase (adenine-specific)DNA methyltransferase
KeywordsTRANSFERASE/DNA/INHIBITOR / DNA Adenine Methylation / PROTEIN-DNA COMPLEX / TRANSFERASE / DNA BINDING PROTEIN / TRANSFERASE-DNA-INHIBITOR complex
Function / homology
Function and homology information


N-methyltransferase activity / site-specific DNA-methyltransferase (adenine-specific) / site-specific DNA-methyltransferase (adenine-specific) activity / hydrolase activity / DNA binding
Similarity search - Function
TaqI-like C-terminal specificity domain / TaqI-like C-terminal specificity domain / N-6 DNA Methylase / DNA methylase, adenine-specific / N-6 Adenine-specific DNA methylases signature. / DNA methylase, N-6 adenine-specific, conserved site / S-adenosyl-L-methionine-dependent methyltransferase superfamily
Similarity search - Domain/homology
: / Chem-YB0 / DNA / DNA (> 10) / site-specific DNA-methyltransferase (adenine-specific)
Similarity search - Component
Biological speciesClostridioides difficile (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / FOURIER SYNTHESIS / Resolution: 2.74 Å
AuthorsZhou, J. / Horton, J.R. / Cheng, X.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM049245-23 United States
CitationJournal: Acs Chem.Biol. / Year: 2023
Title: Comparative Study of Adenosine Analogs as Inhibitors of Protein Arginine Methyltransferases and a Clostridioides difficile- Specific DNA Adenine Methyltransferase.
Authors: Zhou, J. / Deng, Y. / Iyamu, I.D. / Horton, J.R. / Yu, D. / Hajian, T. / Vedadi, M. / Rotili, D. / Mai, A. / Blumenthal, R.M. / Zhang, X. / Huang, R. / Cheng, X.
History
DepositionJan 9, 2023Deposition site: RCSB / Processing site: RCSB
Revision 1.0May 10, 2023Provider: repository / Type: Initial release
Revision 1.1May 17, 2023Group: Refinement description / Category: struct_ncs_dom_lim
Item: _struct_ncs_dom_lim.beg_auth_asym_id / _struct_ncs_dom_lim.beg_auth_comp_id ..._struct_ncs_dom_lim.beg_auth_asym_id / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_auth_seq_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_asym_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_auth_seq_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Site-specific DNA-methyltransferase (adenine-specific)
B: Site-specific DNA-methyltransferase (adenine-specific)
C: Site-specific DNA-methyltransferase (adenine-specific)
E: DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)-3')
D: DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)-3')
F: DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)-3')
G: DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)-3')
H: DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)-3')
I: DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)234,45828
Polymers231,9239
Non-polymers2,53519
Water8,647480
1
A: Site-specific DNA-methyltransferase (adenine-specific)
E: DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)-3')
D: DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)78,0708
Polymers77,3083
Non-polymers7625
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Site-specific DNA-methyltransferase (adenine-specific)
F: DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)-3')
G: DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)78,41914
Polymers77,3083
Non-polymers1,11211
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Site-specific DNA-methyltransferase (adenine-specific)
H: DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)-3')
I: DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)77,9696
Polymers77,3083
Non-polymers6613
Water543
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)80.914, 160.725, 229.624
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number19
Space group name H-MP212121
Space group name HallP2ac2ab
Symmetry operation#1: x,y,z
#2: x+1/2,-y+1/2,-z
#3: -x,y+1/2,-z+1/2
#4: -x+1/2,-y,z+1/2
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and ((resid 31 and (name N or name...
d_2ens_1(chain "B" and ((resid 31 and (name N or name...
d_3ens_1(chain "C" and (resid 31 through 81 or (resid 82...
d_1ens_2chain "D"
d_2ens_2chain "F"
d_3ens_2chain "H"
d_1ens_3chain "E"
d_2ens_3chain "G"
d_3ens_3chain "I"

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
d_11ens_1TYRTYRLYSLYSAA31 - 13031 - 130
d_12ens_1ILEILEILEILEAA141 - 577141 - 577
d_21ens_1TYRTYRLYSLYSBB31 - 13031 - 130
d_22ens_1ILEILEILEILEBB141 - 577141 - 577
d_31ens_1TYRTYRILEILECC31 - 57731 - 577
d_11ens_2DTDTDADADE1 - 141 - 14
d_21ens_2DTDTDADAFF1 - 141 - 14
d_31ens_2DTDTDADAHH1 - 141 - 14
d_11ens_3DADADADAED1 - 141 - 14
d_21ens_3DADADADAGG1 - 141 - 14
d_31ens_3DADADADAII1 - 141 - 14

NCS ensembles :
ID
ens_1
ens_2
ens_3

NCS oper:
IDCodeMatrixVector
1given(-0.609488710278, -0.782953338695, -0.124529440171), (-0.776377141306, 0.621258845654, -0.106188422884), (0.160505596479, 0.0319611658586, -0.986517327459)-26.6434482156, -12.181482189, -18.5700080841
2given(-0.888915770292, 0.0281248728375, -0.457206457582), (-0.434488757171, -0.367891031305, 0.82211660303), (-0.145080230281, 0.929443478977, 0.339244080521)7.65831186046, 12.2593321985, -27.9023445635
3given(-0.613240106115, -0.782806836974, -0.105593694124), (-0.773867415305, 0.622197401172, -0.118319979311), (0.158321810819, 0.00915696247645, -0.987345103931)-26.7955152324, -12.2681907494, -18.7681935761
4given(-0.902436543603, 0.0581845963112, -0.426875669865), (-0.421459290118, -0.324683509696, 0.846730586021), (-0.0893328133637, 0.944031340202, 0.317528702912)7.76036307845, 12.9171237395, -26.7236510655
5given(-0.605284811192, -0.789114534848, -0.104539696922), (-0.786261560098, 0.613184002031, -0.0761455104737), (0.124189598808, 0.0361058242641, -0.991601388161)-26.8170335337, -12.6054052661, -19.5118439618
6given(-0.942693721647, 0.142169937166, -0.30185469374), (-0.333658218677, -0.39934109615, 0.85393142701), (0.00086039304595, 0.905712094372, 0.42389251212)8.20135478098, 14.2408925736, -24.6045862879

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Components

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Protein , 1 types, 3 molecules ABC

#1: Protein Site-specific DNA-methyltransferase (adenine-specific) / DNA methyltransferase


Mass: 68749.023 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Clostridioides difficile (bacteria)
Gene: BN1095_20232, BN1096_690041, BN1097_700039, E5F26_07025, E5F27_14470, E5F28_16275, E5F29_09620, E5F30_09400, E5F31_14185, E5F32_03810, E5F33_08870, E5F36_00990, E5F37_03060, E5F38_04245, E5F39_ ...Gene: BN1095_20232, BN1096_690041, BN1097_700039, E5F26_07025, E5F27_14470, E5F28_16275, E5F29_09620, E5F30_09400, E5F31_14185, E5F32_03810, E5F33_08870, E5F36_00990, E5F37_03060, E5F38_04245, E5F39_00835, E5F40_02785, E5F41_17405, E5F42_11180, E5F43_12285, E5F44_14865, E5F45_19095
Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: A0A031WG99, site-specific DNA-methyltransferase (adenine-specific)

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DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)- ... , 2 types, 6 molecules EGIDFH

#2: DNA chain DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)-3')


Mass: 4325.825 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) Clostridioides difficile (bacteria)
#3: DNA chain DNA (5'-D(*AP*TP*GP*GP*GP*AP*CP*TP*TP*TP*TP*TP*GP*A)-3')


Mass: 4232.795 Da / Num. of mol.: 3 / Source method: obtained synthetically / Source: (synth.) Clostridioides difficile (bacteria)

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Non-polymers , 4 types, 499 molecules

#4: Chemical
ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL / Ethylene glycol


Mass: 62.068 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: C2H6O2
#5: Chemical ChemComp-YB0 / 5'-S-{2-[N'-(cyclohexylmethyl)carbamimidamido]ethyl}-N-(3-phenylpropyl)-5'-thioadenosine


Mass: 582.761 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C29H42N8O3S / Feature type: SUBJECT OF INVESTIGATION
#6: Chemical
ChemComp-K / POTASSIUM ION


Mass: 39.098 Da / Num. of mol.: 9 / Source method: obtained synthetically / Formula: K
#7: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 480 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.38 Å3/Da / Density % sol: 63.64 %
Crystal growTemperature: 292 K / Method: vapor diffusion, sitting drop
Details: 21-24% PEG 3350, 0.1 M Tris-HCl pH 7-7.5, 0.28M Potassium Citrate
PH range: 7-7.5

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Dec 5, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.74→48.55 Å / Num. obs: 79090 / % possible obs: 99.58 % / Redundancy: 13.7 % / Biso Wilson estimate: 48.55 Å2 / CC1/2: 0.961 / Net I/σ(I): 6.35
Reflection shellResolution: 2.75→2.85 Å / Num. unique obs: 7611 / CC1/2: 0.417 / % possible all: 97.35

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Processing

Software
NameVersionClassification
PHENIX1.19.2_4158refinement
HKL-2000data reduction
HKL-2000data scaling
PHENIXphasing
RefinementMethod to determine structure: FOURIER SYNTHESIS / Resolution: 2.74→48.55 Å / SU ML: 0.3449 / Cross valid method: FREE R-VALUE / σ(F): 1.34 / Phase error: 21.7532
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.218 2000 2.53 %
Rwork0.1713 77068 -
obs0.1725 79068 99.61 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 54.72 Å2
Refinement stepCycle: LAST / Resolution: 2.74→48.55 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13309 1704 160 480 15653
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.005915646
X-RAY DIFFRACTIONf_angle_d0.838521444
X-RAY DIFFRACTIONf_chiral_restr0.05192354
X-RAY DIFFRACTIONf_plane_restr0.00512414
X-RAY DIFFRACTIONf_dihedral_angle_d17.48995982
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2BX-RAY DIFFRACTIONTorsion NCS0.716400776669
ens_1d_3CX-RAY DIFFRACTIONTorsion NCS0.902872529774
ens_2d_2FX-RAY DIFFRACTIONTorsion NCS0.91887858079
ens_2d_3HX-RAY DIFFRACTIONTorsion NCS1.38653406627
ens_3d_2GX-RAY DIFFRACTIONTorsion NCS1.0469940504
ens_3d_3IX-RAY DIFFRACTIONTorsion NCS1.29077341636
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.74-2.810.38031370.32855273X-RAY DIFFRACTION96.42
2.81-2.890.3381400.29455405X-RAY DIFFRACTION99.09
2.89-2.970.30291410.24615429X-RAY DIFFRACTION99.86
2.97-3.070.29251410.23325442X-RAY DIFFRACTION100
3.07-3.180.28461430.22645488X-RAY DIFFRACTION100
3.18-3.30.22711420.19665466X-RAY DIFFRACTION100
3.3-3.450.2391420.16425493X-RAY DIFFRACTION99.98
3.45-3.640.18051420.15355491X-RAY DIFFRACTION100
3.64-3.860.2131430.15145506X-RAY DIFFRACTION100
3.86-4.160.20481440.13545519X-RAY DIFFRACTION99.96
4.16-4.580.15341430.12275530X-RAY DIFFRACTION99.84
4.58-5.240.17851440.12655570X-RAY DIFFRACTION100
5.24-6.60.19051460.16025624X-RAY DIFFRACTION99.83
6.6-48.550.21161520.17835832X-RAY DIFFRACTION99.53
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.24277521893-1.54955129072-1.555199522842.985666259540.0440293290185.33719683953-0.134727779213-0.8497177991880.885839828222-0.0779490873256-0.302495817236-0.493015364612-0.5073763487080.8748279758840.1220330337330.413169927931-0.0637086663396-0.1351865822620.4403456667430.04906328703580.82862446431334.553147506136.7571375422-41.3297608798
21.72354599806-0.27631895733-0.04897884591172.27575747871.270375452284.42506925075-0.055551211220.07060107330870.113653988440.284061472485-0.08807054963670.27764690441-0.569244280968-0.4126047396530.1744158554760.5411112479470.0534331957560.08447657739180.360865624175-0.03858965735060.341199047014-34.06832403895.101056387734.540575963
31.50961238012-0.698568485937-0.4317486025543.745743109971.468761343233.360741731840.1446800859040.0140410064710.207010522455-0.117482992957-0.0195301811996-0.122368323876-0.4843574628150.0186982745412-0.1090139520990.367772734968-0.03622034742230.09332144761480.332091775948-0.04481579748050.283085666182-22.6693627668-3.3496027789818.8966930048
40.557563547882-0.337929756745-0.9631352670091.695265802080.6443006028553.40960473479-0.0742219090134-0.1004727240180.04487707516590.377034553687-0.00202704407573-0.04819568518210.1467322665410.1405990573740.07299096536410.286789857614-0.0815790032440.005116129534810.361066011426-0.008771838619380.324695135343-17.8113411091-23.803734725118.4176399416
52.64595870458-0.8981398961731.508180118734.021072619630.3778078565811.693236643060.07925451166290.562279988129-0.0794177723921-1.075742968560.1231165334370.539447336304-0.00569702169815-0.263655827076-0.2147370505020.397610439614-0.0262594737417-0.04776957894890.568637013451-0.02047063577510.339680919797-27.4114577907-27.1130514184-16.6838119393
61.44516800870.3642670673110.1639065028432.26367988781-1.395977697573.1151657646-0.03009904802770.0205370938549-0.187938577232-0.138671854794-0.115000616711-0.2498370282030.1859819452060.2829432136310.1064526350080.1740819326880.00545647530810.06289838245650.326216383674-0.008886275985640.281429723115-14.1811195416-35.28646706123.44404002561
72.71165400068-0.2815409767461.689056541242.16856047716-0.7173169329234.942517656160.01175589705760.1310417544370.1373757332730.0118410304781-0.1777289217880.0041699563954-0.5302220345120.01880555381110.1873321445380.3826109686350.07376324425760.06996764415310.3106507631580.0919588555180.324783503333-13.728787485214.1517274784-57.17479531
81.89049390151-0.616096838860.6012083140372.4481328577-0.9075435592192.594157063690.1361192886960.07285768699190.0732138368026-0.161164964915-0.02065427133390.0629699014257-0.11904679362-0.0930057510685-0.1050804891290.2687982668470.06151739707320.03430169154370.3241815867510.06894240709090.267658386411-9.80029954517-0.55881884789-44.1014788354
91.72921684248-0.976812752417-0.1212394167062.417465329030.2355346981191.848000698940.0337758369085-0.05463780396460.0186558122260.06193203982140.0313911220577-0.09956144928610.02021414569340.156361169607-0.0705130756430.189618486445-0.02551631883810.003022431705980.22777064770.06979241915330.2333586852288.80019318335-18.3593090253-21.5726400125
101.50844232496-0.617172172239-0.2624619845213.481973216341.817947442092.79931003029-0.101327281125-0.4693853440660.1080845639921.002905257820.04440795994250.0516981324901-0.0410592421775-0.09886284464310.05874114454270.8951109285240.09814431211960.07114075861290.490145452333-0.003999364381320.48899855977819.483034280746.266630591-18.6286096253
112.31966908999-1.298100541360.8579024205652.66886169401-0.2653014413062.087312615330.09268861404390.131780438995-0.09510007818-0.353120106823-0.09111615216930.132070762928-0.0943272475253-0.0346657144205-0.0009833045749110.3764047464540.01678833768270.06662959445740.2501050960740.06106800596680.40223382259422.500431909230.7401711992-54.9620294161
120.262843665601-0.8301954907640.4423529725643.10522288487-0.5899241548182.654751719660.0697538591536-0.7796566807780.08011941831710.306428263921-0.2207138296671.09082703046-0.157570783083-0.870360800475-0.2562032490650.267883853308-0.06870523850130.1608716782740.737517815739-0.05736446329150.523507722852-32.3589828436-20.4140757944.17165797805
131.64918840662-0.06366148619831.091693326743.12031162973-0.4364057418020.825055885563-0.2314422915260.398204110409-0.399669828841-0.0380862054959-0.07087856746550.6872710503230.230824988523-1.144250620560.1596029966930.291008691981-0.1449103697040.01257679177630.616458517431-0.1197958257810.464694791321-28.4378022942-19.24318863992.03235171678
141.68340663585-0.532358427003-1.182341471491.135700129930.1608906299050.8433595871030.414787156275-0.4102878568870.4371567414330.061042972022-0.331790116064-0.190708388176-0.5599757106740.858045753874-0.08171310881850.315471665657-0.140399120060.02525457704210.4942333464020.05862674920480.3493778643635.48341340413-2.47460951407-25.4638741747
151.48774451343-0.0515683897104-0.2187128281411.39000941759-1.042632305942.55911575085-0.2165564206690.01528682534990.0850682513201-0.168773871567-0.0377667821087-0.484219089999-0.154140813680.3400018041760.2094891029260.411102537284-0.0709879714030.09880840673880.2615073158190.03831569697590.3931107031778.44593801375-0.00386897214917-28.4153381857
161.00078762855-0.4775128384850.8036523995750.6362448499350.4737223828893.61890961405-0.1291726373360.0491890051220.0586800060811-0.0788612819115-0.00130706484783-1.14460530624-0.4691272770660.4881970394490.132232674080.458681873334-0.02387603810080.1667529839560.4140923890530.1395576746120.82926451737131.425380685332.8737073848-41.6958097013
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'I' and (resid 1 through 14 )IQ1 - 14
22chain 'A' and (resid 30 through 130 )AA30 - 1301 - 101
33chain 'A' and (resid 131 through 273 )AA131 - 273102 - 240
44chain 'A' and (resid 274 through 359 )AA274 - 359241 - 326
55chain 'A' and (resid 360 through 428 )AA360 - 428327 - 395
66chain 'A' and (resid 429 through 577 )AA429 - 577396 - 544
77chain 'B' and (resid 27 through 130 )BD27 - 1301 - 104
88chain 'B' and (resid 131 through 311 )BD131 - 311105 - 285
99chain 'B' and (resid 312 through 577 )BD312 - 577286 - 551
1010chain 'C' and (resid 31 through 311 )CJ31 - 3111 - 271
1111chain 'C' and (resid 312 through 577 )CJ312 - 577272 - 537
1212chain 'E' and (resid 1 through 14 )EL1 - 14
1313chain 'D' and (resid 1 through 14 )DM1 - 14
1414chain 'F' and (resid 1 through 14 )FN1 - 14
1515chain 'G' and (resid 1 through 14 )GO1 - 14
1616chain 'H' and (resid 1 through 14 )HP1 - 14

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