+Open data
-Basic information
Entry | Database: PDB / ID: 8fg7 | |||||||||
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Title | Apo mouse acidic mammalian chitinase, catalytic domain at 277 K | |||||||||
Components | Acidic mammalian chitinase | |||||||||
Keywords | HYDROLASE / GH18 chitinase / acidic mammalian chitinase | |||||||||
Function / homology | Function and homology information Digestion of dietary carbohydrate / production of molecular mediator involved in inflammatory response / chitinase / chitinase activity / chitin catabolic process / immune system process / chitin binding / polysaccharide catabolic process / positive regulation of chemokine production / kinase binding ...Digestion of dietary carbohydrate / production of molecular mediator involved in inflammatory response / chitinase / chitinase activity / chitin catabolic process / immune system process / chitin binding / polysaccharide catabolic process / positive regulation of chemokine production / kinase binding / apoptotic process / extracellular space / extracellular region / cytoplasm Similarity search - Function | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.64 Å | |||||||||
Authors | Diaz, R.E. / Asthana, P. / Fraser, J.S. | |||||||||
Funding support | United States, 2items
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Citation | Journal: Biorxiv / Year: 2024 Title: Structural characterization of ligand binding and pH-specific enzymatic activity of mouse Acidic Mammalian Chitinase. Authors: Diaz, R.E. / Ecker, A.K. / Correy, G.J. / Asthana, P. / Young, I.D. / Faust, B. / Thompson, M.C. / Seiple, I.B. / Van Dyken, S.J. / Locksley, R.M. / Fraser, J.S. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8fg7.cif.gz | 173.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8fg7.ent.gz | 125.8 KB | Display | PDB format |
PDBx/mmJSON format | 8fg7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fg/8fg7 ftp://data.pdbj.org/pub/pdb/validation_reports/fg/8fg7 | HTTPS FTP |
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-Related structure data
Related structure data | 8fg5C 8fr9C 8fraC 8frbC 8frcC 8frdC 8frgC 8gcaC C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 44621.992 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Chia, Chia1 / Plasmid: mAMCase_CatD_6xHis_pTwistCMV / Cell line (production host): ExpiCHO-S / Production host: Cricetulus griseus (Chinese hamster) / References: UniProt: Q91XA9, chitinase |
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#2: Chemical | ChemComp-MG / |
#3: Water | ChemComp-HOH / |
Has ligand of interest | N |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.16 Å3/Da / Density % sol: 43.1 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop Details: 11 mg/mL AMCase catalytic domain 19.8% PEG 3350 0.198 M Magnesium Formate 0.01 M Phenol |
-Data collection
Diffraction | Mean temperature: 277 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: ALS / Beamline: 8.3.1 / Wavelength: 1.1158 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Oct 20, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1158 Å / Relative weight: 1 |
Reflection | Resolution: 1.64→54.78 Å / Num. obs: 47999 / % possible obs: 99.37 % / Redundancy: 12.9 % / Biso Wilson estimate: 16.6 Å2 / CC1/2: 0.996 / CC star: 0.999 / Rmerge(I) obs: 0.2489 / Rpim(I) all: 0.07097 / Rrim(I) all: 0.2591 / Net I/σ(I): 7 |
Reflection shell | Resolution: 1.64→1.699 Å / Redundancy: 13.2 % / Mean I/σ(I) obs: 1.19 / Num. unique obs: 4688 / CC1/2: 0.502 / % possible all: 98.65 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.64→54.78 Å / SU ML: 0.1929 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 16.3817 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 19.41 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.64→54.78 Å
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Refine LS restraints |
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LS refinement shell |
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