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- PDB-8emd: Human PU.1 ETS-Domain (165-270) Q226E Mutant Bound to d(AATAAGCGG... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8emd | |||||||||||||||
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Title | Human PU.1 ETS-Domain (165-270) Q226E Mutant Bound to d(AATAAGCGGAAGTGGG) | |||||||||||||||
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![]() | TRANSCRIPTION/DNA / transcription factor / protein-DNA complex / ETS family / ETS / PU.1 / TRANSCRIPTION-DNA complex | |||||||||||||||
Function / homology | ![]() positive regulation of antifungal innate immune response / regulation of myeloid progenitor cell differentiation / pro-T cell differentiation / negative regulation of neutrophil degranulation / myeloid leukocyte differentiation / positive regulation of microglial cell mediated cytotoxicity / TRAIL-activated apoptotic signaling pathway / STAT family protein binding / endothelial to hematopoietic transition / negative regulation of adipose tissue development ...positive regulation of antifungal innate immune response / regulation of myeloid progenitor cell differentiation / pro-T cell differentiation / negative regulation of neutrophil degranulation / myeloid leukocyte differentiation / positive regulation of microglial cell mediated cytotoxicity / TRAIL-activated apoptotic signaling pathway / STAT family protein binding / endothelial to hematopoietic transition / negative regulation of adipose tissue development / pericyte cell differentiation / defense response to tumor cell / negative regulation of non-canonical NF-kappaB signal transduction / regulation of DNA-binding transcription factor activity / oncogene-induced cell senescence / negative regulation of protein localization to chromatin / positive regulation of p38MAPK cascade / DNA-binding transcription repressor activity / positive regulation of B cell differentiation / DNA-binding transcription activator activity / negative regulation of NF-kappaB transcription factor activity / interleukin-6-mediated signaling pathway / NFAT protein binding / cis-regulatory region sequence-specific DNA binding / protein sequestering activity / transcription initiation-coupled chromatin remodeling / positive regulation of miRNA transcription / Transcriptional regulation of granulopoiesis / regulation of erythrocyte differentiation / histone deacetylase binding / RUNX1 regulates transcription of genes involved in differentiation of HSCs / DNA-binding transcription factor binding / transcription regulator complex / sequence-specific DNA binding / RNA polymerase II-specific DNA-binding transcription factor binding / transcription cis-regulatory region binding / molecular adaptor activity / DNA-binding transcription factor activity, RNA polymerase II-specific / RNA polymerase II cis-regulatory region sequence-specific DNA binding / DNA-binding transcription factor activity / negative regulation of gene expression / negative regulation of DNA-templated transcription / chromatin binding / regulation of transcription by RNA polymerase II / chromatin / regulation of DNA-templated transcription / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / RNA binding / nucleoplasm / nucleus Similarity search - Function | |||||||||||||||
Biological species | ![]() synthetic construct (others) | |||||||||||||||
Method | ![]() ![]() ![]() | |||||||||||||||
![]() | Terrell, J.R. / Poon, G.M.K. | |||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: DNA selection by the master transcription factor PU.1. Authors: Terrell, J.R. / Taylor, S.J. / Schneider, A.L. / Lu, Y. / Vernon, T.N. / Xhani, S. / Gumpper, R.H. / Luo, M. / Wilson, W.D. / Steidl, U. / Poon, G.M.K. | |||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 146.3 KB | Display | ![]() |
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PDB format | ![]() | 91.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 8e3kSC ![]() 8e3rC ![]() 8e4hC ![]() 8e5yC ![]() 8ebhC ![]() 8ee9C ![]() 8ej6C ![]() 8ej8C ![]() 8ek3C ![]() 8ek8C ![]() 8ekjC ![]() 8ekuC ![]() 8ekvC ![]() 8ekzC ![]() 8em9C ![]() 8engC ![]() 8eo1C ![]() 8eo4C ![]() 8eqgC ![]() 8eqkC ![]() 8eqlC S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: DNA chain | Mass: 5036.292 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) | ||||
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#2: DNA chain | Mass: 4760.091 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) | ||||
#3: Protein | Mass: 12437.568 Da / Num. of mol.: 1 / Fragment: ETS-Domain (165-270) / Mutation: Q226E Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||
#4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.51 Å3/Da / Density % sol: 51.07 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 4.6 / Details: 100 mM Sodium Acetate, pH=4.6, 2% PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 22, 2021 |
Radiation | Monochromator: Liquid nitrogen cooled dual crystal / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.55→33.26 Å / Num. obs: 28806 / % possible obs: 96.84 % / Redundancy: 3.3 % / Biso Wilson estimate: 23.48 Å2 / CC1/2: 0.998 / CC star: 0.999 / Rmerge(I) obs: 0.04523 / Rpim(I) all: 0.02917 / Rrim(I) all: 0.054 / Net I/σ(I): 14.1 |
Reflection shell | Resolution: 1.55→1.605 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.4951 / Mean I/σ(I) obs: 2.29 / Num. unique obs: 2825 / CC1/2: 0.838 / CC star: 0.955 / Rpim(I) all: 0.3202 / Rrim(I) all: 0.5912 / % possible all: 96.35 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 8E3K Resolution: 1.55→33.26 Å / SU ML: 0.1727 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 20.6263 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 29.9 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.55→33.26 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 6.19988270096 Å / Origin y: 0.964197386164 Å / Origin z: 5.05471647587 Å
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Refinement TLS group | Selection details: all |