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Open data
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Basic information
Entry | Database: PDB / ID: 8elb | ||||||
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Title | CTX-M-14 beta-lactamase mutant- N132A | ||||||
![]() | Beta-lactamase | ||||||
![]() | HYDROLASE / beta-lactamase hydrolase antibiotic beta-lactam | ||||||
Function / homology | ![]() beta-lactam antibiotic catabolic process / beta-lactamase activity / beta-lactamase / response to antibiotic / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lu, S. / Neetu, N. / Palzkill, T. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Mutagenesis and structural analysis reveal the CTX-M beta-lactamase active site is optimized for cephalosporin catalysis and drug resistance. Authors: Lu, S. / Montoya, M. / Hu, L. / Neetu, N. / Sankaran, B. / Prasad, B.V.V. / Palzkill, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 124.2 KB | Display | ![]() |
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PDB format | ![]() | 94 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 449.6 KB | Display | ![]() |
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Full document | ![]() | 458.8 KB | Display | |
Data in XML | ![]() | 28 KB | Display | |
Data in CIF | ![]() | 41.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8dodC ![]() 8doeC ![]() 8donC ![]() 8dp4C ![]() 8dpqC ![]() 8elaC ![]() 1yltS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 27957.520 Da / Num. of mol.: 2 / Mutation: N132A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: blaCTX-M-14, beta-lactamase CTX-M-14, bla, bla CTX-M-14, bla-CTX-M-14a, blaCTX-M, blaCTX-M-14a, blaCTX-M-14b, blaCTX-M-14c, blaCTX-M-27b, blatoho-3, blaUOE-2, CTX-M-14 Plasmid: pET28a / Production host: ![]() ![]() #2: Chemical | ChemComp-PEG / #3: Water | ChemComp-HOH / | Has ligand of interest | N | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.78 % |
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Crystal grow | Temperature: 296 K / Method: vapor diffusion, hanging drop / pH: 8.5 / Details: 0.1 M Tris-HCl, PEG 10,000 (20% w/v) |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 11, 2019 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.00001 Å / Relative weight: 1 |
Reflection | Resolution: 1.5→42.29 Å / Num. obs: 69726 / % possible obs: 95.3 % / Redundancy: 5.9 % / Biso Wilson estimate: 10.36 Å2 / CC1/2: 0.975 / Rmerge(I) obs: 0.183 / Rpim(I) all: 0.084 / Net I/σ(I): 6 |
Reflection shell | Resolution: 1.5→1.52 Å / Rmerge(I) obs: 0.118 / Mean I/σ(I) obs: 7.9 / Num. unique obs: 2475 / CC1/2: 0.975 / Rpim(I) all: 0.058 / Rrim(I) all: 0.132 / % possible all: 95.3 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1YLT Resolution: 1.5→42.29 Å / Cor.coef. Fo:Fc: 0.948 / Cor.coef. Fo:Fc free: 0.925 / SU B: 1.628 / SU ML: 0.062 / Cross valid method: THROUGHOUT / ESU R: 0.091 / ESU R Free: 0.091 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 13.21 Å2
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Refinement step | Cycle: 1 / Resolution: 1.5→42.29 Å
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Refine LS restraints |
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