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- PDB-8eee: Crystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ10... -

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Basic information

Entry
Database: PDB / ID: 8eee
TitleCrystal structure of a NHP anti-ZIKV neutralizing antibody rhMZ104-d in complex with ZIKV E glycoprotein
Components
  • Envelope protein E
  • rhMZ104-D antibody heavy chain
  • rhMZ104-D antibody light chain
KeywordsVIRAL PROTEIN/IMMUNE SYSTEM / ZIKV / ZIKV-specific / cross-protomer epitopes / VIRAL PROTEIN / VIRAL PROTEIN-IMMUNE SYSTEM complex
Function / homology
Function and homology information


flavivirin / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of host TYK2 activity / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT2 activity / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / negative regulation of innate immune response / viral capsid / double-stranded RNA binding / nucleoside-triphosphate phosphatase / 4 iron, 4 sulfur cluster binding / clathrin-dependent endocytosis of virus by host cell ...flavivirin / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of host TYK2 activity / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT2 activity / symbiont-mediated suppression of host JAK-STAT cascade via inhibition of STAT1 activity / negative regulation of innate immune response / viral capsid / double-stranded RNA binding / nucleoside-triphosphate phosphatase / 4 iron, 4 sulfur cluster binding / clathrin-dependent endocytosis of virus by host cell / mRNA (guanine-N7)-methyltransferase / molecular adaptor activity / methyltransferase cap1 / methyltransferase cap1 activity / mRNA 5'-cap (guanine-N7-)-methyltransferase activity / RNA helicase activity / protein dimerization activity / host cell perinuclear region of cytoplasm / host cell endoplasmic reticulum membrane / RNA helicase / symbiont-mediated suppression of host type I interferon-mediated signaling pathway / symbiont-mediated activation of host autophagy / RNA-directed RNA polymerase / serine-type endopeptidase activity / viral RNA genome replication / RNA-directed RNA polymerase activity / fusion of virus membrane with host endosome membrane / viral envelope / lipid binding / centrosome / GTP binding / virion attachment to host cell / host cell nucleus / virion membrane / structural molecule activity / ATP hydrolysis activity / proteolysis / extracellular region / ATP binding / metal ion binding / membrane
Similarity search - Function
Immunoglobulin-like - #350 / Flavivirus capsid protein C superfamily / Flavivirus non-structural protein NS2B / Genome polyprotein, Flavivirus / : / Flavivirus non-structural protein NS4A / Flavivirus non-structural protein NS2B / Flavivirus non-structural protein NS4B / mRNA cap 0/1 methyltransferase / Flavivirus non-structural protein NS4B ...Immunoglobulin-like - #350 / Flavivirus capsid protein C superfamily / Flavivirus non-structural protein NS2B / Genome polyprotein, Flavivirus / : / Flavivirus non-structural protein NS4A / Flavivirus non-structural protein NS2B / Flavivirus non-structural protein NS4B / mRNA cap 0/1 methyltransferase / Flavivirus non-structural protein NS4B / Flavivirus non-structural protein NS4A / Flavivirus NS2B domain profile. / mRNA cap 0 and cap 1 methyltransferase (EC 2.1.1.56 and EC 2.1.1.57) domain profile. / Flavivirus non-structural protein NS2A / Flavivirus non-structural protein NS2A / Flavivirus NS3, petidase S7 / Peptidase S7, Flavivirus NS3 serine protease / Flavivirus NS3 protease (NS3pro) domain profile. / RNA-directed RNA polymerase, thumb domain, Flavivirus / Flavivirus RNA-directed RNA polymerase, thumb domain / RNA-directed RNA polymerase, flavivirus / Flavivirus RNA-directed RNA polymerase, fingers and palm domains / Flavivirus capsid protein C / Flavivirus capsid protein C / Flavivirus non-structural Protein NS1 / Flavivirus non-structural protein NS1 / Envelope glycoprotein M, flavivirus / Envelope glycoprotein M superfamily, flavivirus / Flavivirus envelope glycoprotein M / Flavivirus polyprotein propeptide / Flavivirus polyprotein propeptide superfamily / Flavivirus polyprotein propeptide / Flavivirus envelope glycoprotein E, stem/anchor domain / : / Flavivirus NS3 helicase, C-terminal helical domain / Flavivirus envelope glycoprotein E, Stem/Anchor domain / Flavivirus envelope glycoprotein E, Stem/Anchor domain superfamily / Flavivirus glycoprotein E, immunoglobulin-like domain / Flavivirus glycoprotein, immunoglobulin-like domain / Flavivirus glycoprotein central and dimerisation domain / Flavivirus glycoprotein, central and dimerisation domains / Flaviviral glycoprotein E, central domain, subdomain 1 / Flaviviral glycoprotein E, central domain, subdomain 2 / Ribosomal RNA methyltransferase, FtsJ domain / FtsJ-like methyltransferase / Flavivirus/Alphavirus glycoprotein, immunoglobulin-like domain superfamily / Flavivirus glycoprotein, central and dimerisation domain superfamily / Flaviviral glycoprotein E, dimerisation domain / DEAD box, Flavivirus / Flavivirus DEAD domain / helicase superfamily c-terminal domain / Immunoglobulin E-set / Superfamilies 1 and 2 helicase C-terminal domain profile. / Superfamilies 1 and 2 helicase ATP-binding type-1 domain profile. / DEAD-like helicases superfamily / Helicase, C-terminal / Helicase superfamily 1/2, ATP-binding domain / RNA-directed RNA polymerase, catalytic domain / RdRp of positive ssRNA viruses catalytic domain profile. / S-adenosyl-L-methionine-dependent methyltransferase superfamily / Immunoglobulins / Peptidase S1, PA clan / DNA/RNA polymerase superfamily / Immunoglobulin-like / Sandwich / P-loop containing nucleoside triphosphate hydrolase / Mainly Beta
Similarity search - Domain/homology
Biological speciesZika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013
Macaca mulatta (Rhesus monkey)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.819 Å
AuthorsSankhala, R.S. / Joyce, M.G.
Funding support United States, 1items
OrganizationGrant numberCountry
Department of Defense (DOD, United States)W81XWH-07-2-0067 United States
CitationJournal: Cell Rep / Year: 2023
Title: Zika-specific neutralizing antibodies targeting inter-dimer envelope epitopes.
Authors: Sankhala, R.S. / Dussupt, V. / Donofrio, G. / Gromowski, G.D. / De La Barrera, R.A. / Larocca, R.A. / Mendez-Rivera, L. / Lee, A. / Choe, M. / Zaky, W. / Mantus, G. / Jensen, J.L. / Chen, W. ...Authors: Sankhala, R.S. / Dussupt, V. / Donofrio, G. / Gromowski, G.D. / De La Barrera, R.A. / Larocca, R.A. / Mendez-Rivera, L. / Lee, A. / Choe, M. / Zaky, W. / Mantus, G. / Jensen, J.L. / Chen, W.H. / Gohain, N. / Bai, H. / McCracken, M.K. / Mason, R.D. / Leggat, D. / Slike, B.M. / Tran, U. / Jian, N. / Abbink, P. / Peterson, R. / Mendes, E.A. / Freitas de Oliveira Franca, R. / Calvet, G.A. / Bispo de Filippis, A.M. / McDermott, A. / Roederer, M. / Hernandez, M. / Albertus, A. / Davidson, E. / Doranz, B.J. / Rolland, M. / Robb, M.L. / Lynch, R.M. / Barouch, D.H. / Jarman, R.G. / Thomas, S.J. / Modjarrad, K. / Michael, N.L. / Krebs, S.J. / Joyce, M.G.
History
DepositionSep 7, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 30, 2023Provider: repository / Type: Initial release
Revision 1.1Oct 23, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
Z: Envelope protein E
E: Envelope protein E
L: rhMZ104-D antibody light chain
H: rhMZ104-D antibody heavy chain
A: rhMZ104-D antibody heavy chain
B: rhMZ104-D antibody light chain
hetero molecules


Theoretical massNumber of molelcules
Total (without water)184,6498
Polymers184,4576
Non-polymers1922
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: gel filtration
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)85.320, 130.420, 109.710
Angle α, β, γ (deg.)90.000, 104.170, 90.000
Int Tables number4
Space group name H-MP1211
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain E and (resid 1 through 10 or (resid 11...
21(chain Z and (resid 1 through 151 or resid 163 through 403))

NCS domain segments:

Ens-ID: 1

Dom-IDComponent-IDBeg auth comp-IDBeg label comp-IDEnd auth comp-IDEnd label comp-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11ILEILEASPASP(chain E and (resid 1 through 10 or (resid 11...EB1 - 101 - 10
12PHEPHEPHEPHE(chain E and (resid 1 through 10 or (resid 11...EB1111
13ILEILESERSER(chain E and (resid 1 through 10 or (resid 11...EB1 - 4031 - 403
14ILEILESERSER(chain E and (resid 1 through 10 or (resid 11...EB1 - 4031 - 403
15ILEILESERSER(chain E and (resid 1 through 10 or (resid 11...EB1 - 4031 - 403
16ILEILESERSER(chain E and (resid 1 through 10 or (resid 11...EB1 - 4031 - 403
21ILEILEMETMET(chain Z and (resid 1 through 151 or resid 163 through 403))ZA1 - 1511 - 151
22ASNASNSERSER(chain Z and (resid 1 through 151 or resid 163 through 403))ZA163 - 403163 - 403

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Components

#1: Protein Envelope protein E


Mass: 44214.059 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Zika virus ZIKV/H. sapiens/FrenchPolynesia/10087PF/2013
Strain: isolate ZIKV/Human/French Polynesia/10087PF/2013
Production host: Insect cell expression vector pTIE1 (others)
References: UniProt: A0A024B7W1
#2: Antibody rhMZ104-D antibody light chain


Mass: 23542.021 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Macaca mulatta (Rhesus monkey) / Production host: Homo sapiens (human)
#3: Antibody rhMZ104-D antibody heavy chain


Mass: 24472.354 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Macaca mulatta (Rhesus monkey) / Production host: Homo sapiens (human)
#4: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: SO4
Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.21 Å3/Da / Density % sol: 61.66 %
Crystal growTemperature: 291 K / Method: vapor diffusion, hanging drop
Details: 12% PEG 8000, 0.2 M (NH4)2SO4, 0.1M Tris-HCl (pH 8.5)

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.97 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Oct 20, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97 Å / Relative weight: 1
ReflectionResolution: 2.8→50 Å / Num. obs: 50533 / % possible obs: 89 % / Redundancy: 4.6 % / CC1/2: 0.93 / Rsym value: 0.089 / Net I/σ(I): 14.5
Reflection shellResolution: 2.8→2.9 Å / Num. unique obs: 1890 / CC1/2: 0.798

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Processing

Software
NameVersionClassification
PHENIX1.11.1_2575refinement
PDB_EXTRACT3.27data extraction
HKL-2000data reduction
HKL-2000data scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 5IRE

5ire


Resolution: 2.819→14.989 Å / SU ML: 0.39 / Cross valid method: THROUGHOUT / σ(F): 1.38 / Phase error: 29.78 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2751 1982 4.85 %
Rwork0.2168 38844 -
obs0.2196 40826 73.1 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 203.91 Å2 / Biso mean: 55.2936 Å2 / Biso min: 8.52 Å2
Refinement stepCycle: final / Resolution: 2.819→14.989 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11021 0 10 0 11031
Biso mean--81.62 --
Num. residues----1468
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00311302
X-RAY DIFFRACTIONf_angle_d0.83615383
X-RAY DIFFRACTIONf_chiral_restr0.0461709
X-RAY DIFFRACTIONf_plane_restr0.0051972
X-RAY DIFFRACTIONf_dihedral_angle_d14.7116696
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11E3408X-RAY DIFFRACTION17.516TORSIONAL
12Z3408X-RAY DIFFRACTION17.516TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork% reflection obs (%)
2.819-2.88870.4365100.289820733.6
2.8887-2.96620.4365230.31844664.4
2.9662-3.05280.3303510.320399726
3.0528-3.15040.3836790.3091155541
3.1504-3.26190.38251240.2899242864
3.2619-3.39110.33631560.2713304581
3.3911-3.54350.29931810.2516354493
3.5435-3.72760.32181920.23483764100
3.7276-3.95710.30481930.22073795100
3.9571-4.25610.27321940.19723802100
4.2561-4.67260.22671930.17413791100
4.6726-5.32210.22511950.17713814100
5.3221-6.60820.26881950.22073811100
6.6082-14.9890.22531960.19813845100
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.38230.05070.28980.18-0.01920.1575-0.12280.8969-0.0546-0.120.12050.1644-0.11330.24880.08950.1889-0.024-0.10691.236-0.07920.218514.87665.6261.1241
21.6452-0.17081.87151.4745-0.32683.9319-0.0260.7360.1499-0.31290.01490.059-0.3518-0.21880.0790.27880.1103-0.03620.73050.11640.070315.3388.353717.3707
30.39480.2531-0.09541.33531.05612.88-0.04060.10.0091-0.06340.1529-0.0249-0.10890.2414-0.02620.348-0.0535-0.01071.1365-0.00210.1265-9.58593.268-35.0983
40.77180.45660.40391.4886-0.36330.5067-0.0079-0.24640.30190.2419-0.14560.1549-0.1412-0.18110.09820.2913-0.0962-0.0050.1768-0.30380.47895.657621.279765.6447
50.2498-0.0466-0.42870.31350.30640.81030.10490.540.015-0.0127-0.05780.11440.0422-0.2772-0.05340.17230.0483-0.02540.6827-0.09050.0819-11.58024.037812.012
60.29620.37680.64050.47860.96693.4434-0.0467-0.12030.09550.1519-0.22580.27120.1937-0.21650.0270.33070.00370.09890.1618-0.11390.3997-2.826712.501355.1671
71.46040.37641.45721.07021.77936.92680.08980.79850.0016-0.03590.023-0.0417-0.0065-0.1871-0.00210.12280.0449-0.00770.47050.03930.2048-5.58918.726221.0948
82.21780.3156-0.31121.01130.31120.17510.003-0.78160.16470.2471-0.17940.18180.31550.21140.17930.50510.0134-0.03060.38510.05320.33018.881613.37271.2948
93.158-0.52460.50873.0464-0.59734.93720.007-1.00290.12550.9796-0.1611-0.31270.08740.38630.0730.4564-0.0431-0.08530.4499-0.12590.198923.673216.879375.5894
103.9489-0.52480.24423.0005-1.22842.14321.0336-0.2613-3.24210.3903-0.1792-0.29471.08330.3164-0.83481.0134-0.1205-0.6361.0892-0.13492.3746-30.2989-26.2558-0.7485
116.64630.42662.70431.01521.29592.37530.53231.068-1.9851-1.12890.10980.29921.1067-0.1969-0.64250.97850.1243-0.40031.3002-0.68631.3921-23.5497-17.7917-3.3916
125.66933.45331.97672.23520.42755.19950.1035-0.1843-2.0524-0.15870.09030.55211.1407-0.7106-0.12350.5289-0.0747-0.09390.4712-0.08321.4433-32.1153-15.42836.9118
137.122.36871.60172.1281-1.9128.15960.7258-0.4453-2.85980.19290.1401-0.08461.1478-0.0659-0.83030.48840.0422-0.24920.78960.22921.3738-21.1847-13.47912.4405
140.5701-0.06240.56170.2744-0.22681.38750.67490.0249-2.21410.34890.58990.50130.8855-0.6198-1.27220.7611-0.1537-0.33551.24890.43621.999-31.5377-21.62117.3502
159.213-3.81225.74866.75270.35416.7799-0.02821.1302-3.05540.06890.22661.49110.7317-2.0452-0.20170.56030.1370.00811.0879-0.24811.9492-20.4182-20.3866.3349
165.7918-5.93551.29017.74421.18074.0565-0.33460.7004-2.3172-2.06920.18420.27051.99980.28620.18681.16280.063-0.11720.7177-0.52451.6249-21.2277-20.99160.9463
172.33330.57512.49661.12370.97092.80010.60680.209-1.4523-0.0355-0.2096-0.02580.82120.6898-0.43761.5069-0.2544-1.02271.50410.80523.5479-35.1675-30.533710.6632
189.04724.9670.10885.7989-3.83867.0488-0.22731.0367-1.9434-1.63450.71610.10831.0525-1.0373-0.45980.49570.0857-0.19650.8849-0.32630.9148-32.3786-13.48011.165
196.27470.65540.73262.69112.19225.03340.28130.4593-1.5913-0.51870.03570.82481.065-0.376-0.29330.7501-0.2992-0.30741.0761-0.05781.0199-34.1743-17.1905-0.663
200.6757-0.04340.60720.41210.18380.72480.37650.0089-0.7681-0.0066-0.13180.06250.07750.1297-0.25150.4777-0.0416-0.01781.9699-0.12990.7522-54.1006-5.62251.5174
212.6383-0.41531.95060.23030.36494.14560.241-0.51130.04080.16830.0093-0.04220.3176-0.62220.12020.3513-0.0034-0.081.0554-0.47970.6115-42.6986-1.62663.0568
224.8884-0.21752.16520.9614-0.46811.44-0.05040.38870.3137-0.1274-0.32670.2578-0.0903-0.08310.44850.42930.0911-0.08121.6078-0.39020.5719-47.76560.7818-3.0285
233.8568-1.04331.64890.4337-0.71431.18090.2945-0.69420.18880.0704-0.42110.3463-0.3184-1.07040.14190.60860.14740.00211.3974-0.51970.75-36.2509-3.19989.1579
243.81841.62334.98331.47591.75586.66960.1537-1.1851-0.368-0.044-0.22170.65320.2845-1.1920.08450.4959-0.14240.03291.2223-0.30530.8042-42.4358-8.58945.2147
250.89930.01940.03490.8673-0.23950.0844-0.3070.41970.4263-0.07660.1273-0.1665-0.32250.10330.09780.368-0.1685-0.06730.10150.13590.238255.99345.505939.4547
261.68380.320.33371.4564-0.10661.7463-0.03580.2755-0.235-0.1054-0.0256-0.37860.1579-0.09270.09140.0903-0.05920.11560.13990.03990.365560.2946-8.77838.6827
270.68481.15660.61493.164-0.10122.0154-0.01870.1211-0.4197-0.63110.1327-0.6450.290.169-0.11520.4564-0.12850.04090.08590.02670.788777.1447-18.620641.4955
281.2821-0.48470.04250.24160.27891.4921-0.0461-0.2413-0.61210.00030.12510.32130.48790.0137-0.09830.25530.0019-0.03380.25960.24440.411140.3794-17.804148.0306
292.6709-0.4865-0.53860.7274-0.00221.0765-0.0516-0.1207-0.09180.11070.03020.1311-0.0656-0.20150.06270.1040.10560.12170.1122-0.05760.197236.2393-8.299243.52
300.6524-0.0256-0.11872.48760.07060.61310.0050.0182-0.25570.0882-0.0324-0.33260.1120.0349-0.07370.1678-0.036-0.07890.0606-0.06880.281343.2119-11.436.1346
313.5874-2.5256-1.40294.58961.23496.4249-0.11520.2517-0.8267-0.17250.14960.05560.6812-0.44150.02910.159-0.0307-0.02730.14290.00160.276434.1158-14.477537.178
322.7598-0.95130.56085.7883-0.08112.5190.11330.0006-0.29650.1902-0.1481-0.2960.2278-0.02510.00670.2505-0.05940.0520.0451-0.02130.192443.3742-11.609441.3515
330.3288-0.1130.1480.0661-0.0910.11820.0744-0.0891-0.306-0.0097-0.0601-0.08780.10370.0275-0.00570.1785-0.02590.0580.06350.02770.433448.5184-20.64546.1983
343.39282.70361.34632.6941.94742.0366-0.19130.14310.1377-0.18060.26-0.7166-0.56010.39910.10160.3715-0.1107-0.07630.28070.18550.880383.9531-24.870251.2074
350.94860.7749-0.02631.4082-0.16560.53480.0359-0.1002-0.04780.22-0.0357-0.2991-0.08990.08680.04860.3812-0.0065-0.12770.08230.09270.612570.9418-27.562357.3724
360.22920.2003-0.02981.3201-0.06450.3321-0.01320.01510.0207-0.0710.02890.01430.0051-0.02030.06860.3323-0.0675-0.17160.12530.20950.648166.0228-23.667150.3664
370.118-0.3351-0.24334.1777-0.80791.2003-0.0054-0.18940.05240.7785-0.1617-1.4223-0.11350.43580.230.5918-0.0369-0.23560.23730.11010.930180.1563-30.515660.1829
381.7723-0.1158-0.93944.4818-0.58281.144-0.0969-0.0869-0.11250.1547-0.0435-0.1124-0.07340.06030.04850.52090.1057-0.13430.23420.13060.606172.0575-36.037756.1399
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'Z' and (resid 1 through 209 )Z1 - 209
2X-RAY DIFFRACTION2chain 'Z' and (resid 210 through 279 )Z210 - 279
3X-RAY DIFFRACTION3chain 'Z' and (resid 280 through 403 )Z280 - 403
4X-RAY DIFFRACTION4chain 'E' and (resid 1 through 40 )E1 - 40
5X-RAY DIFFRACTION5chain 'E' and (resid 41 through 129 )E41 - 129
6X-RAY DIFFRACTION6chain 'E' and (resid 130 through 209 )E130 - 209
7X-RAY DIFFRACTION7chain 'E' and (resid 210 through 281 )E210 - 281
8X-RAY DIFFRACTION8chain 'E' and (resid 282 through 316 )E282 - 316
9X-RAY DIFFRACTION9chain 'E' and (resid 317 through 403 )E317 - 403
10X-RAY DIFFRACTION10chain 'L' and (resid 2 through 17 )L2 - 17
11X-RAY DIFFRACTION11chain 'L' and (resid 18 through 29 )L18 - 29
12X-RAY DIFFRACTION12chain 'L' and (resid 30 through 52 )L30 - 52
13X-RAY DIFFRACTION13chain 'L' and (resid 53 through 60 )L53 - 60
14X-RAY DIFFRACTION14chain 'L' and (resid 61 through 67 )L61 - 67
15X-RAY DIFFRACTION15chain 'L' and (resid 68 through 73 )L68 - 73
16X-RAY DIFFRACTION16chain 'L' and (resid 74 through 83 )L74 - 83
17X-RAY DIFFRACTION17chain 'L' and (resid 84 through 92 )L84 - 92
18X-RAY DIFFRACTION18chain 'L' and (resid 93 through 100 )L93 - 100
19X-RAY DIFFRACTION19chain 'L' and (resid 101 through 116 )L101 - 116
20X-RAY DIFFRACTION20chain 'H' and (resid 2 through 16 )H2 - 16
21X-RAY DIFFRACTION21chain 'H' and (resid 17 through 60 )H17 - 60
22X-RAY DIFFRACTION22chain 'H' and (resid 61 through 91 )H61 - 91
23X-RAY DIFFRACTION23chain 'H' and (resid 92 through 108 )H92 - 108
24X-RAY DIFFRACTION24chain 'H' and (resid 109 through 126 )H109 - 126
25X-RAY DIFFRACTION25chain 'A' and (resid 2 through 91 )A2 - 91
26X-RAY DIFFRACTION26chain 'A' and (resid 92 through 148 )A92 - 148
27X-RAY DIFFRACTION27chain 'A' and (resid 149 through 227 )A149 - 227
28X-RAY DIFFRACTION28chain 'B' and (resid 2 through 17 )B2 - 17
29X-RAY DIFFRACTION29chain 'B' and (resid 18 through 40 )B18 - 40
30X-RAY DIFFRACTION30chain 'B' and (resid 41 through 54 )B41 - 54
31X-RAY DIFFRACTION31chain 'B' and (resid 55 through 83 )B55 - 83
32X-RAY DIFFRACTION32chain 'B' and (resid 84 through 104 )B84 - 104
33X-RAY DIFFRACTION33chain 'B' and (resid 105 through 123 )B105 - 123
34X-RAY DIFFRACTION34chain 'B' and (resid 124 through 138 )B124 - 138
35X-RAY DIFFRACTION35chain 'B' and (resid 139 through 171 )B139 - 171
36X-RAY DIFFRACTION36chain 'B' and (resid 172 through 191 )B172 - 191
37X-RAY DIFFRACTION37chain 'B' and (resid 192 through 207 )B192 - 207
38X-RAY DIFFRACTION38chain 'B' and (resid 208 through 218 )B208 - 218

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