- PDB-8eb5: Tandem of Hermes transposase BED domain in complex with the quasi... -
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Basic information
Entry
Database: PDB / ID: 8eb5
Title
Tandem of Hermes transposase BED domain in complex with the quasi palindrome of its transposon left-end
Components
(Hermes transposon left-end subterminal repeats 1 and 2) x 2
Hermes transposase BED domain
Keywords
DNA BINDING PROTEIN/DNA / BED domain / zinc-binding domain / transposase / DNA BINDING PROTEIN-DNA complex
Function / homology
Function and homology information
nucleic acid metabolic process / protein dimerization activity / regulation of transcription by RNA polymerase II / DNA binding / identical protein binding / nucleus / metal ion binding Similarity search - Function
Hermes trasposase, DNA-binding domain / Hermes transposase DNA-binding domain / HAT, C-terminal dimerisation domain / hAT family C-terminal dimerisation region / BED zinc finger / Zinc finger, BED-type / Zinc finger BED-type profile. / Ribonuclease H-like superfamily Similarity search - Domain/homology
National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Disease (NIH/NIDDK)
DK036153-16
United States
Citation
Journal: Nat Commun / Year: 2023 Title: Zinc-finger BED domains drive the formation of the active Hermes transpososome by asymmetric DNA binding. Authors: Laurie Lannes / Christopher M Furman / Alison B Hickman / Fred Dyda / Abstract: The Hermes DNA transposon is a member of the eukaryotic hAT superfamily, and its transposase forms a ring-shaped tetramer of dimers. Our investigation, combining biochemical, crystallography and cryo- ...The Hermes DNA transposon is a member of the eukaryotic hAT superfamily, and its transposase forms a ring-shaped tetramer of dimers. Our investigation, combining biochemical, crystallography and cryo-electron microscopy, and in-cell assays, shows that the full-length Hermes octamer extensively interacts with its transposon left-end through multiple BED domains of three Hermes protomers contributed by three dimers explaining the role of the unusual higher-order assembly. By contrast, the right-end is bound to no BED domains at all. Thus, this work supports a model in which Hermes multimerizes to gather enough BED domains to find its left-end among the abundant genomic DNA, facilitating the subsequent interaction with the right-end.
History
Deposition
Aug 30, 2022
Deposition site: RCSB / Processing site: RCSB
Revision 1.0
Aug 2, 2023
Provider: repository / Type: Initial release
Revision 1.1
May 22, 2024
Group: Data collection / Category: chem_comp_atom / chem_comp_bond
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