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- PDB-8e8v: Structure of the short LOR domain of human AASS bound to N-ethyls... -

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Basic information

Entry
Database: PDB / ID: 8e8v
TitleStructure of the short LOR domain of human AASS bound to N-ethylsuccinimide
ComponentsAlpha-aminoadipic semialdehyde synthase, mitochondrial
KeywordsOXIDOREDUCTASE / lysine metabolism / ketoglutarate / mitochondrial / reductase
Function / homology
Function and homology information


saccharopine dehydrogenase (NADP+, L-lysine-forming) / saccharopine dehydrogenase (NAD+, L-glutamate-forming) / saccharopine dehydrogenase (NADP+, L-lysine-forming) activity / saccharopine dehydrogenase (NAD+, L-glutamate-forming) activity / saccharopine dehydrogenase activity / saccharopine dehydrogenase (NAD+, L-lysine-forming) activity / lysine catabolic process / L-lysine catabolic process to acetyl-CoA via saccharopine / lysine biosynthetic process via aminoadipic acid / Lysine catabolism ...saccharopine dehydrogenase (NADP+, L-lysine-forming) / saccharopine dehydrogenase (NAD+, L-glutamate-forming) / saccharopine dehydrogenase (NADP+, L-lysine-forming) activity / saccharopine dehydrogenase (NAD+, L-glutamate-forming) activity / saccharopine dehydrogenase activity / saccharopine dehydrogenase (NAD+, L-lysine-forming) activity / lysine catabolic process / L-lysine catabolic process to acetyl-CoA via saccharopine / lysine biosynthetic process via aminoadipic acid / Lysine catabolism / transcription corepressor activity / histone binding / mitochondrial matrix / intracellular membrane-bounded organelle / negative regulation of transcription by RNA polymerase II / mitochondrion / nucleus / cytosol / cytoplasm
Similarity search - Function
: / Saccharopine dehydrogenase, NADP binding domain / Saccharopine dehydrogenase-like, C-terminal / Saccharopine dehydrogenase NADP binding domain / Saccharopine dehydrogenase C-terminal domain / Alanine dehydrogenase/pyridine nucleotide transhydrogenase, N-terminal / Alanine dehydrogenase/PNT, N-terminal domain / Alanine dehydrogenase/PNT, C-terminal domain / Alanine dehydrogenase/PNT, N-terminal domain / Alanine dehydrogenase/pyridine nucleotide transhydrogenase, NAD(H)-binding domain / NAD(P)-binding domain superfamily
Similarity search - Domain/homology
1-ETHYL-PYRROLIDINE-2,5-DIONE / Alpha-aminoadipic semialdehyde synthase, mitochondrial
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.45 Å
AuthorsKhamrui, S. / Lazarus, M.B.
Funding support United States, 2items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35GM124838 United States
National Institutes of Health/Eunice Kennedy Shriver National Institute of Child Health & Human Development (NIH/NICHD)R21HD102745 United States
CitationJournal: Open Biology / Year: 2022
Title: Characterization and structure of the human lysine-2-oxoglutarate reductase domain, a novel therapeutic target for treatment of glutaric aciduria type 1.
Authors: Leandro, J. / Khamrui, S. / Suebsuwong, C. / Chen, P.J. / Secor, C. / Dodatko, T. / Yu, C. / Sanchez, R. / DeVita, R.J. / Houten, S.M. / Lazarus, M.B.
History
DepositionAug 25, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Oct 5, 2022Provider: repository / Type: Initial release
Revision 1.1Oct 18, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.2Nov 6, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Alpha-aminoadipic semialdehyde synthase, mitochondrial
B: Alpha-aminoadipic semialdehyde synthase, mitochondrial
C: Alpha-aminoadipic semialdehyde synthase, mitochondrial
D: Alpha-aminoadipic semialdehyde synthase, mitochondrial
hetero molecules


Theoretical massNumber of molelcules
Total (without water)193,8978
Polymers193,3894
Non-polymers5094
Water00
1
A: Alpha-aminoadipic semialdehyde synthase, mitochondrial
hetero molecules


Theoretical massNumber of molelcules
Total (without water)48,4742
Polymers48,3471
Non-polymers1271
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Alpha-aminoadipic semialdehyde synthase, mitochondrial
hetero molecules


Theoretical massNumber of molelcules
Total (without water)48,4742
Polymers48,3471
Non-polymers1271
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Alpha-aminoadipic semialdehyde synthase, mitochondrial
hetero molecules


Theoretical massNumber of molelcules
Total (without water)48,4742
Polymers48,3471
Non-polymers1271
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Alpha-aminoadipic semialdehyde synthase, mitochondrial
hetero molecules


Theoretical massNumber of molelcules
Total (without water)48,4742
Polymers48,3471
Non-polymers1271
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)77.099, 155.963, 78.352
Angle α, β, γ (deg.)90.000, 100.200, 90.000
Int Tables number4
Space group name H-MP1211

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Components

#1: Protein
Alpha-aminoadipic semialdehyde synthase, mitochondrial / LKR/SDH


Mass: 48347.137 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: AASS / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: Q9UDR5, saccharopine dehydrogenase (NADP+, L-lysine-forming), saccharopine dehydrogenase (NAD+, L-glutamate-forming)
#2: Chemical
ChemComp-NEN / 1-ETHYL-PYRROLIDINE-2,5-DIONE


Mass: 127.141 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: C6H9NO2 / Feature type: SUBJECT OF INVESTIGATION
Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.4 Å3/Da / Density % sol: 48.69 %
Crystal growTemperature: 300 K / Method: vapor diffusion, hanging drop / pH: 7.5 / Details: 0.2 M NaCl, 0.1M HEPES, 25% PEG 4000

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: NSLS-II / Beamline: 17-ID-1 / Wavelength: 0.9201 Å
DetectorType: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Jun 16, 2022
RadiationMonochromator: si / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9201 Å / Relative weight: 1
ReflectionResolution: 2.45→47.49 Å / Num. obs: 66842 / % possible obs: 99.9 % / Redundancy: 5.2 % / CC1/2: 0.994 / Rmerge(I) obs: 0.148 / Rpim(I) all: 0.071 / Rrim(I) all: 0.164 / Net I/σ(I): 6.5 / Num. measured all: 350595 / Scaling rejects: 164
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsNum. measured allNum. unique obsCC1/2Rpim(I) allRrim(I) allNet I/σ(I) obs% possible all
2.45-2.515.22.3782325244520.4281.1442.6440.899.2
11.49-47.495.10.06934126700.9920.0330.07719.198.7

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Processing

Software
NameVersionClassification
Aimless0.7.4data scaling
PHENIX1.14_3260refinement
PDB_EXTRACT3.27data extraction
autoPROCdata reduction
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 8E8T

8e8t


Resolution: 2.45→47.49 Å / SU ML: 0.36 / Cross valid method: THROUGHOUT / σ(F): 1.35 / Phase error: 31.52 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2578 3247 4.87 %
Rwork0.234 63424 -
obs0.2351 66671 99.81 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 233.49 Å2 / Biso mean: 84.5537 Å2 / Biso min: 37.13 Å2
Refinement stepCycle: final / Resolution: 2.45→47.49 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12703 0 36 0 12739
Biso mean--72.59 --
Num. residues----1616
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / % reflection obs: 100 %

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection Rwork
2.45-2.48660.39941120.39532789
2.4866-2.52540.3661300.39222753
2.5254-2.56680.40811770.35932720
2.5668-2.61110.41091210.36032767
2.6111-2.65860.33321370.33432751
2.6586-2.70970.33451310.33132749
2.7097-2.7650.361750.32562735
2.765-2.82510.34191230.31372730
2.8251-2.89080.331440.2962757
2.8908-2.96310.33991560.29382730
2.9631-3.04320.28521380.27842733
3.0432-3.13270.27361570.26732767
3.1327-3.23380.30191540.26872731
3.2338-3.34940.28361470.27492748
3.3494-3.48350.29041540.2522747
3.4835-3.64190.25921580.23532719
3.6419-3.83390.21991300.21392788
3.8339-4.0740.23151560.19782755
4.074-4.38830.23621160.20132789
4.3883-4.82950.19791110.18382792
4.8295-5.52750.22611350.20142787
5.5275-6.96050.22541500.21382774
6.9605-47.490.20031350.18032813
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.3613-1.15351.51795.3451.19623.014-0.38090.9897-0.7419-1.04410.36670.3485-0.26330.11190.12120.7438-0.12070.05250.9759-0.39411.0528-9.358-12.261-33.406
22.84760.7950.15663.8871-1.17691.1018-0.1090.3308-0.46840.08930.0734-0.43240.43440.25670.07120.60620.04680.01060.7847-0.25720.7499-7.725-15.576-24.522
34.47280.1982-0.08966.5057-0.06266.68771.1612.0509-1.1166-2.16330.2961-0.68081.71440.8559-1.57981.32490.1558-0.15931.3516-0.39611.1923-11.308-15.914-41.468
45.0926-1.5635-1.1048.27061.89014.8160.96010.5733-0.3787-1.6616-0.8712-0.28930.9068-0.40060.0071.01250.0416-0.00841.0086-0.28170.9759-13.541-23.133-36.736
50.041-0.2354-0.2943.48953.79734.1759-0.34860.5947-0.5439-0.7804-0.17930.11031.81340.45930.25821.7549-0.04660.15611.6024-0.61651.4534-17.944-24.548-46.981
61.7186-0.2184-2.45080.07850.25483.56550.72710.9217-0.7531-0.88640.39080.46360.1215-0.4677-0.48011.8502-0.36820.0371.8901-0.1121.3928-14.323-31.028-43.831
72.5594-0.8304-0.20686.64712.59044.2360.0507-0.0191-0.5036-0.17950.05330.84110.23860.0482-0.08860.7428-0.0593-0.05311.0883-0.32261.183-27.757-16.142-30.735
82.79760.47320.25780.83860.47851.0056-0.08830.2408-0.1778-0.03170.05250.1180.0754-0.09260.04920.3875-0.02880.02870.762-0.03210.4996-34.79211.814-27.468
95.3080.57490.32414.5468-0.23195.0876-0.13141.0919-0.5294-1.5233-0.19320.24150.57090.11320.27260.7475-0.0086-0.06051.2111-0.14460.5752-40.0027.101-43.172
103.00110.1248-0.05217.82460.60772.61050.08040.38130.1501-0.6835-0.28560.0807-0.3064-0.2160.17110.435-0.0123-0.00140.81440.05720.5705-31.68220.493-34.728
118.7203-1.84982.06935.94651.73871.35170.31330.838-0.2686-1.0258-0.2415-1.1792-0.09870.12690.17490.7128-0.14510.22911.3631-0.240.6824-31.9135.168-43.531
124.6704-0.9331.07227.2277-0.60626.76890.53971.87870.7242-1.4586-0.4476-0.59230.38390.9796-0.22110.6452-0.17390.12161.249-0.07930.5736-24.28312.958-44.098
133.4250.5742-0.13114.0867-1.83225.0605-0.18981.5512-0.3449-0.75610.2748-0.39540.16291.2485-0.19880.736-0.09450.11831.6289-0.0690.5116-23.72913.398-41.039
141.6877-0.42250.85482.98880.15711.34430.21570.4383-0.38760.1614-0.15120.83870.5408-0.02110.08660.5541-0.02210.00720.759-0.21490.7305-19.585-18.242-26.726
152.9911-2.06350.37393.897-0.03452.7220.53380.2704-0.86040.8362-0.71330.60390.4845-0.77680.25660.936-0.2151-0.03780.8949-0.28671.2706-26.238-27.125-32.255
163.06150.92650.0354.14820.33381.5497-0.1915-0.25880.4109-0.1530.26550.4989-0.2363-0.3157-0.09840.49180.02780.02930.67710.06310.6541-18.54841.85-24.299
173.89590.92090.22321.25421.4233.5450.48330.64051.1405-1.7951-0.66940.3727-0.9854-0.7055-0.29091.09310.1246-0.16721.03360.23360.8461-14.05248.103-37.144
188.1992-4.57971.42486.2792-3.03884.45620.17560.80490.3789-0.29540.0280.1862-0.39930.1562-0.16941.33770.1576-0.13192.35480.65111.7169-9.5551.763-46.486
190.5403-1.64621.31625.3673-4.41063.7091-0.41571.15110.845-0.8189-0.2786-0.1918-0.9609-0.4280.08151.39670.17680.00461.53240.58861.0031-11.88456.734-41.383
208.34610.14542.29385.10650.33354.8673-0.69631.01810.556-0.22340.3551-1.0868-0.01480.67090.50750.8585-0.13020.10360.66970.06130.79180.96846.392-29.63
211.91840.2026-0.22681.8816-0.60910.53190.04310.1547-0.04740.034-0.0908-0.0872-0.04080.07930.05790.402-0.0229-0.00710.8483-0.04210.50911.8313.436-24.07
223.27741.2731-0.07372.26720.17061.40960.18730.2789-0.0359-0.1896-0.4302-0.4051-0.26860.23070.27950.4863-0.02560.05250.98540.13650.53320.89724.05-34.451
231.91342.9475-2.044.814-2.9212.3738-0.0766-0.86541.62611.188-0.54340.5069-1.22980.06990.52310.93440.3009-0.14151.21130.1240.799210.99631.353-36.816
246.697-0.14150.82183.67140.73366.4154-0.37911.98240.0989-1.5562-0.33240.001-0.1065-0.23280.63780.8532-0.00870.10061.46550.07380.647513.36918.915-43.898
252.95290.4630.17767.94890.51912.47930.1080.0268-0.5251-0.70230.0156-1.07850.20110.139-0.10420.4340.0982-0.01341.1006-0.12620.50954.6457.364-35.307
268.209-4.0002-2.80855.3805-0.72343.68020.4490.93031.3094-1.30640.66880.7434-0.403-0.9895-1.76660.8122-0.0558-0.09651.37980.21130.86144.95324.305-41.829
273.05261.64082.52135.4754-1.72084.17290.39962.1372-0.5855-1.4881-0.04380.5837-0.2972-0.7874-0.22990.74710.0083-0.13021.2073-0.04010.5413-2.95615.527-43.905
284.1115-1.7024-0.30074.84010.55613.63460.21610.63210.0014-1.99-0.10880.8004-0.5524-1.2541-0.03830.69190.0648-0.16061.27730.03570.5937-3.40915.671-40.133
291.9769-0.5334-0.37563.52071.05741.1854-0.0451-0.11010.33190.01050.1947-0.1206-0.30340.4143-0.1310.5378-0.02360.02190.73820.01350.5922-7.89242.095-23.162
302.8658-1.6863-0.12145.64530.22722.6050.09470.31420.8910.163-0.0707-0.7905-0.6469-0.22320.05890.895-0.09320.04680.91210.01090.9328-1.48956.847-29.742
313.195-0.206-0.0863.3214-0.39281.9446-0.1229-0.1851-0.50680.20520.12660.8592-0.157-0.4969-0.00580.4511-0.03440.11740.88880.06930.8998-41.0063.358-0.308
325.9037-0.0796-1.18881.4770.2572.56890.3687-1.9051-0.68941.0120.0641.3248-0.0905-0.7969-0.44870.8619-0.08430.31531.25050.24191.1506-43.309-3.62512.167
338.6415-4.2039-0.48817.82784.07662.59390.0131-0.45050.2980.92570.72111.2423-0.2867-1.246-0.40462.3453-0.2723-0.51141.41490.31431.6295-46.571-10.6921.02
340.4527-1.03540.82024.26720.76835.0668-0.7654-0.4058-1.02521.279-0.37960.3656-0.1139-0.79781.02730.9084-0.19040.29831.1840.10611.4803-43.842-11.61811.631
354.4482-0.17450.95085.72310.28353.43420.13710.5734-1.1931.0542-0.05690.68360.7101-0.241-0.16930.677-0.0255-0.060.92960.08411.6708-29.04-16.0260.462
361.53520.39650.00152.25270.71362.7240.13780.13980.13130.12870.0859-0.03810.05220.1764-0.22220.3767-0.0168-0.0270.54840.03510.4086-6.6015.81-0.452
375.0818-1.250.80914.685-0.42753.82870.43060.5682-0.5341-0.0127-0.1429-0.25090.55740.6546-0.30130.5345-0.0201-0.03210.704-0.10640.5404-3.96-9.339-2.185
380.77820.06790.41353.3576-0.60861.68450.1170.115-0.32020.1944-0.0341-0.06140.52150.3252-0.08420.63680.1281-0.08180.604-0.05880.5665-4.127-19.8256.443
398.0605-0.07741.45117.28810.03585.77180.0495-1.3082-0.19741.15330.08061.81060.1852-1.0163-0.10850.8870.03770.0890.62410.07510.7083-12.415-17.56715.471
403.0813-2.5399-0.18862.83661.59853.6944-0.3612-0.81280.17231.2083-0.1183-0.1182-0.15041.41850.35740.70620.0819-0.17780.8990.03840.77784.679-6.21617.888
417.75750.15441.63126.53280.17266.3423-0.5962-1.0333-0.34610.02080.09-0.5501-0.47591.10660.4140.53940.1683-0.07880.7417-0.00030.4253-2.767-3.28210.025
420.5382-0.03451.16754.003-1.21692.8893-0.2694-0.8208-0.25771.43850.6650.42290.1247-0.2506-0.44730.87970.06750.05810.83660.00850.5619-12.525-1.89616.459
433.68681.7296-2.58013.09780.76463.50020.1448-0.97021.28281.39010.6711-0.067-1.5732-0.2333-0.6340.92870.1520.15240.8208-0.16980.7765-5.9880.74623.007
446.0103-0.46612.7816.36210.11977.1396-0.0433-0.6522-0.41131.11360.25880.326-0.4884-0.7995-0.37850.86860.11240.05330.73430.00430.36-9.8371.05113.966
453.18710.3260.44490.8182-0.16981.27220.0437-0.0256-1.66330.276-0.06280.49130.7067-0.14110.23230.7655-0.15460.12850.80870.06011.3521-39.626-11.0480.781
464.49141.23240.48454.56280.75862.7227-0.01710.3375-0.31680.0693-0.1173-0.7738-0.09130.7090.16020.5041-0.1214-0.10911.03960.02670.682713.80121.0871.149
474.31071.72850.33864.4481-0.65220.6477-0.5427-0.2746-0.26991.0441-0.3566-0.4588-0.71211.48271.12441.0503-0.2487-0.38451.49460.01521.009317.14226.76714.343
480.4512-0.8231-0.81891.89271.89241.95570.349-1.0626-0.63371.10010.05410.30740.8841-0.3948-0.40771.85250.4861-0.29521.50430.00241.721120.46831.44522.69
494.41820.7062-0.54343.63320.26622.24640.2782-0.14250.02530.5976-0.5013-0.723-0.01720.09450.09320.8776-0.2765-0.26570.8593-0.00740.74577.05538.0497.047
504.1729-0.140.34611.6456-0.58522.9547-0.0809-0.46430.35970.30260.10450.1614-0.3749-0.1009-0.01310.4597-0.0040.01940.5862-0.08470.4165-22.41226.4390.3
513.0341-0.7081-0.31011.89492.71734.07170.43870.05330.031-0.1813-0.14040.16590.02620.3351-0.30052.42780.3464-0.21131.362-0.79531.4271-24.78355.47715.71
520.70420.68260.37680.63640.06930.57070.0684-0.18940.69240.76120.3524-0.3063-0.7914-0.4380.1421.55880.0589-0.13321.1765-0.78451.6331-19.36350.614.233
534.4068-2.4533-0.28221.8043-1.25624.43980.7404-1.28022.30270.2978-0.4116-0.1659-1.19360.1275-0.15940.8751-0.02770.09170.9244-0.49491.1028-22.10534.7519.468
543.8651.13971.90512.7508-0.05121.0749-0.6502-0.98050.63060.68940.59210.2467-0.39710.27840.21111.336-0.1425-0.01791.5791-0.38540.6998-23.84134.118.683
555.07780.22632.1914.63840.2015.2382-0.5987-1.82950.78270.21560.21460.5618-0.4726-0.49120.31930.68990.1112-0.06821.2576-0.21940.6533-23.4927.47711.654
561.66320.79250.95080.38750.50530.8311-0.4123-1.52060.57951.338-0.20590.37110.2293-0.15340.39891.2101-0.01-0.12822.0851-0.21210.6632-13.22726.89118.302
574.74322.16951.49212.255-1.53574.43930.5587-1.8703-0.18940.361-0.10150.6298-0.02560.6368-0.30270.8926-0.09630.0111.61370.02040.6575-20.19321.54621.392
581.8319-0.7673-1.2444.18820.71850.8672-0.0482-0.9488-0.25991.0425-0.4671-0.97050.38620.69770.01871.3342-0.221-0.01121.90390.14850.6798-21.38416.35718.363
597.957-1.5398-7.32392.2991.09626.803-0.1756-2.14420.56940.115-0.052-0.3638-0.31230.79850.12060.85570.0267-0.01481.5476-0.04930.4424-12.80124.67413.034
603.2504-0.57560.76252.04680.80430.9292-0.0950.30560.41860.40190.0407-0.7061-0.60450.49570.04240.8468-0.2997-0.14680.97670.0550.704512.97635.4543.35
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND RESID 24:36 )A24 - 36
2X-RAY DIFFRACTION2( CHAIN A AND RESID 37:91 )A37 - 91
3X-RAY DIFFRACTION3( CHAIN A AND RESID 92:100 )A92 - 100
4X-RAY DIFFRACTION4( CHAIN A AND RESID 101:109 )A101 - 109
5X-RAY DIFFRACTION5( CHAIN A AND RESID 110:126 )A110 - 126
6X-RAY DIFFRACTION6( CHAIN A AND RESID 127:131 )A127 - 131
7X-RAY DIFFRACTION7( CHAIN A AND RESID 132:152 )A132 - 152
8X-RAY DIFFRACTION8( CHAIN A AND RESID 153:309 )A153 - 309
9X-RAY DIFFRACTION9( CHAIN A AND RESID 310:330 )A310 - 330
10X-RAY DIFFRACTION10( CHAIN A AND RESID 340:356 )A340 - 356
11X-RAY DIFFRACTION11( CHAIN A AND RESID 357:369 )A357 - 369
12X-RAY DIFFRACTION12( CHAIN A AND RESID 370:385 )A370 - 385
13X-RAY DIFFRACTION13( CHAIN A AND RESID 386:402 )A386 - 402
14X-RAY DIFFRACTION14( CHAIN A AND RESID 403:437 )A403 - 437
15X-RAY DIFFRACTION15( CHAIN A AND RESID 438:450 )A438 - 450
16X-RAY DIFFRACTION16( CHAIN B AND RESID 24:93 )B24 - 93
17X-RAY DIFFRACTION17( CHAIN B AND RESID 94:110 )B94 - 110
18X-RAY DIFFRACTION18( CHAIN B AND RESID 123:125 )B123 - 125
19X-RAY DIFFRACTION19( CHAIN B AND RESID 126:131 )B126 - 131
20X-RAY DIFFRACTION20( CHAIN B AND RESID 132:143 )B132 - 143
21X-RAY DIFFRACTION21( CHAIN B AND RESID 144:264 )B144 - 264
22X-RAY DIFFRACTION22( CHAIN B AND RESID 265:309 )B265 - 309
23X-RAY DIFFRACTION23( CHAIN B AND RESID 310:313 )B310 - 313
24X-RAY DIFFRACTION24( CHAIN B AND RESID 314:330 )B314 - 330
25X-RAY DIFFRACTION25( CHAIN B AND RESID 340:356 )B340 - 356
26X-RAY DIFFRACTION26( CHAIN B AND RESID 357:369 )B357 - 369
27X-RAY DIFFRACTION27( CHAIN B AND RESID 370:386 )B370 - 386
28X-RAY DIFFRACTION28( CHAIN B AND RESID 387:402 )B387 - 402
29X-RAY DIFFRACTION29( CHAIN B AND RESID 403:432 )B403 - 432
30X-RAY DIFFRACTION30( CHAIN B AND RESID 433:450 )B433 - 450
31X-RAY DIFFRACTION31( CHAIN C AND RESID 24:92 )C24 - 92
32X-RAY DIFFRACTION32( CHAIN C AND RESID 93:110 )C93 - 110
33X-RAY DIFFRACTION33( CHAIN C AND RESID 123:126 )C123 - 126
34X-RAY DIFFRACTION34( CHAIN C AND RESID 127:138 )C127 - 138
35X-RAY DIFFRACTION35( CHAIN C AND RESID 139:152 )C139 - 152
36X-RAY DIFFRACTION36( CHAIN C AND RESID 153:215 )C153 - 215
37X-RAY DIFFRACTION37( CHAIN C AND RESID 216:253 )C216 - 253
38X-RAY DIFFRACTION38( CHAIN C AND RESID 254:309 )C254 - 309
39X-RAY DIFFRACTION39( CHAIN C AND RESID 310:322 )C310 - 322
40X-RAY DIFFRACTION40( CHAIN C AND RESID 323:341 )C323 - 341
41X-RAY DIFFRACTION41( CHAIN C AND RESID 342:358 )C342 - 358
42X-RAY DIFFRACTION42( CHAIN C AND RESID 361:379 )C361 - 379
43X-RAY DIFFRACTION43( CHAIN C AND RESID 380:389 )C380 - 389
44X-RAY DIFFRACTION44( CHAIN C AND RESID 390:402 )C390 - 402
45X-RAY DIFFRACTION45( CHAIN C AND RESID 403:450 )C403 - 450
46X-RAY DIFFRACTION46( CHAIN D AND RESID 24:93 )D24 - 93
47X-RAY DIFFRACTION47( CHAIN D AND RESID 94:110 )D94 - 110
48X-RAY DIFFRACTION48( CHAIN D AND RESID 124:129 )D124 - 129
49X-RAY DIFFRACTION49( CHAIN D AND RESID 130:152 )D130 - 152
50X-RAY DIFFRACTION50( CHAIN D AND RESID 153:271 )D153 - 271
51X-RAY DIFFRACTION51( CHAIN D AND RESID 272:278 )D272 - 278
52X-RAY DIFFRACTION52( CHAIN D AND RESID 279:298 )D279 - 298
53X-RAY DIFFRACTION53( CHAIN D AND RESID 299:311 )D299 - 311
54X-RAY DIFFRACTION54( CHAIN D AND RESID 312:343 )D312 - 343
55X-RAY DIFFRACTION55( CHAIN D AND RESID 344:357 )D344 - 357
56X-RAY DIFFRACTION56( CHAIN D AND RESID 361:373 )D361 - 373
57X-RAY DIFFRACTION57( CHAIN D AND RESID 374:386 )D374 - 386
58X-RAY DIFFRACTION58( CHAIN D AND RESID 387:394 )D387 - 394
59X-RAY DIFFRACTION59( CHAIN D AND RESID 395:403 )D395 - 403
60X-RAY DIFFRACTION60( CHAIN D AND RESID 404:450 )D404 - 450

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