登録情報 データベース : PDB / ID : 8.0E+73 構造の表示 ダウンロードとリンクタイトル Vigna radiata supercomplex I+III2 (full bridge) 要素(MPP-alpha (protomer ...) x 2 COB (cyt b) CYC1 (cyt c1) MPP-beta NDUA1 NDUA11 NDUA12 NDUA13 NDUA2 NDUA3 NDUA5 NDUA6 NDUA7 NDUA8 NDUA9 NDUAB1-alpha NDUAB1-beta NDUB10 NDUB11 NDUB2 NDUB3 NDUB4 NDUB6 NDUB7 NDUB8 NDUB9 NDUC2 NDUCA1 NDUCA2 NDUCAL2 NDUFX NDUP1 NDUP2 NDUP4 NDUS1 NDUS2 NDUS3 NDUS4 NDUS5 NDUS6 NDUS7 NDUS8 NDUV1 NDUV2 NDUX1 Nad1 Nad2 Nad3 Nad4 Nad4L Nad5 Nad6 QCR10 (UCRY) QCR6 QCR7 QCR8 QCR9 UCR1 (Rieske iron-sulfur protein subunit) 残り54件を表示 表示を減らす 詳細キーワード ELECTRON TRANSPORT / respiratory supercomplex / nadh-cyt c oxidoreductase / membrane complex機能・相同性 機能・相同性情報分子機能 ドメイン・相同性 構成要素
TIM22 mitochondrial import inner membrane insertion complex / P450-containing electron transport chain / protein processing involved in protein targeting to mitochondrion / protein insertion into mitochondrial inner membrane / : / quinol-cytochrome-c reductase / oxidoreductase activity, acting on NAD(P)H / ubiquinol-cytochrome-c reductase activity / mitochondrial electron transport, ubiquinol to cytochrome c / NADH:ubiquinone reductase (H+-translocating) ... TIM22 mitochondrial import inner membrane insertion complex / P450-containing electron transport chain / protein processing involved in protein targeting to mitochondrion / protein insertion into mitochondrial inner membrane / : / quinol-cytochrome-c reductase / oxidoreductase activity, acting on NAD(P)H / ubiquinol-cytochrome-c reductase activity / mitochondrial electron transport, ubiquinol to cytochrome c / NADH:ubiquinone reductase (H+-translocating) / : / mitochondrial electron transport, NADH to ubiquinone / : / mitochondrial respiratory chain complex I assembly / NADH dehydrogenase (ubiquinone) activity / catalytic activity / quinone binding / ATP synthesis coupled electron transport / respiratory electron transport chain / mitochondrial membrane / electron transport chain / mitochondrial intermembrane space / metalloendopeptidase activity / fatty acid biosynthetic process / 2 iron, 2 sulfur cluster binding / 4 iron, 4 sulfur cluster binding / mitochondrial inner membrane / membrane => GO:0016020 / oxidoreductase activity / electron transfer activity / heme binding / mitochondrion / proteolysis / RNA binding / membrane / metal ion binding 類似検索 - 分子機能 Cytochrome b-c1 complex subunit 8, plants / Cytochrome b-c1 complex subunit 8 / Mitochondrial import inner membrane translocase subunit TIM22 / Putative NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, plant/fungi / At2g27730-like / NADH-ubiquinone oxidoreductase 11kDa subunit / NADH-ubiquinone oxidoreductase 11 kDa subunit / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit ... Cytochrome b-c1 complex subunit 8, plants / Cytochrome b-c1 complex subunit 8 / Mitochondrial import inner membrane translocase subunit TIM22 / Putative NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, plant/fungi / At2g27730-like / NADH-ubiquinone oxidoreductase 11kDa subunit / NADH-ubiquinone oxidoreductase 11 kDa subunit / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / Hexapeptide repeat of succinyl-transferase / Adrenodoxin / NAD(P)H-quinone oxidoreductase subunit 3, bacterial/plastid / Cytochrome b-c1 complex, subunit 6 / Cytochrome b-c1 complex subunit 7 / Cytochrome b-c1 complex subunit 7 superfamily / Ubiquinol-cytochrome C reductase complex 14kD subunit / Cytochrome b-c1 complex subunit 9 / Cytochrome b-c1 complex subunit 8 superfamily / Cytochrome b-c1 complex subunit 9 superfamily / Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like / Cytochrome b-c1 complex subunit Rieske, transmembrane domain / Ubiquinol cytochrome reductase transmembrane region / Ubiquinol-cytochrome C reductase hinge domain / Ubiquinol-cytochrome C reductase hinge domain superfamily / Ubiquinol-cytochrome C reductase hinge protein / Cytochrome c1, transmembrane anchor, C-terminal / Ubiquinol-cytochrome c reductase, iron-sulphur subunit / Cytochrome c1 / Cytochrome C1 family / Peptidase M16, zinc-binding site / Insulinase family, zinc-binding region signature. / Hexapeptide repeat / Rieske iron-sulphur protein, C-terminal / Rieske iron-sulphur protein / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase MWFE subunit / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / Bacterial transferase hexapeptide (six repeats) / Zinc finger, CHCC-type / Zinc-finger domain / Peptidase M16, C-terminal / Peptidase M16 inactive domain / Peptidase M16, N-terminal / Insulinase (Peptidase family M16) / Metalloenzyme, LuxS/M16 peptidase-like / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NDUFB9, LYR domain / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / ETC complex I subunit conserved region / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / Trimeric LpxA-like superfamily / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / Rieske [2Fe-2S] iron-sulphur domain / Rieske [2Fe-2S] domain / Rieske [2Fe-2S] iron-sulfur domain profile. / Rieske [2Fe-2S] iron-sulphur domain superfamily / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. / Complex 1 LYR protein domain / NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site / Complex 1 protein (LYR family) / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH:ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 3 superfamily / NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 / Respiratory-chain NADH dehydrogenase 24 Kd subunit signature. / CHCH / CHCH domain / NuoE domain / NADH:ubiquinone oxidoreductase, subunit G, iron-sulphur binding / His(Cys)3-ligated-type [4Fe-4S] domain profile. / NADH-quinone oxidoreductase subunit E-like / NADH-quinone oxidoreductase subunit E, N-terminal / NAD-dependent epimerase/dehydratase / Molybdopterin oxidoreductase, 4Fe-4S domain / Prokaryotic molybdopterin oxidoreductases 4Fe-4S domain profile. / NAD dependent epimerase/dehydratase family / NADH:ubiquinone oxidoreductase-like, 20kDa subunit / NADH ubiquinone oxidoreductase, 20 Kd subunit 類似検索 - ドメイン・相同性 1,2-Distearoyl-sn-glycerophosphoethanolamine / CARDIOLIPIN / : / FE2/S2 (INORGANIC) CLUSTER / FLAVIN MONONUCLEOTIDE / HEME C / PROTOPORPHYRIN IX CONTAINING FE / Chem-NDP / 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / IRON/SULFUR CLUSTER ... 1,2-Distearoyl-sn-glycerophosphoethanolamine / CARDIOLIPIN / : / FE2/S2 (INORGANIC) CLUSTER / FLAVIN MONONUCLEOTIDE / HEME C / PROTOPORPHYRIN IX CONTAINING FE / Chem-NDP / 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / IRON/SULFUR CLUSTER / UBIQUINONE-10 / Chem-ZMP / Acyl carrier protein / NADH dehydrogenase [ubiquinone] flavoprotein / Copper ion binding / NADH dehydrogenase [ubiquinone] iron-sulfur protein / Cytochrome b-c1 complex subunit Rieske, mitochondrial / uncharacterized protein LOC106754061 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2 / outer envelope pore protein 16-3, chloroplastic/mitochondrial / serine/arginine-rich-splicing factor SR34 isoform X2 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial / Complex III subunit 9 / uncharacterized protein LOC106758628 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / Probable mitochondrial-processing peptidase subunit beta, mitochondrial isoform X1 / probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / uncharacterized protein LOC106761134 / gamma carbonic anhydrase 1, mitochondrial-like / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / Cytochrome b-c1 complex subunit 7 / Cytochrome b-c1 complex subunit 8 / mitochondrial-processing peptidase subunit alpha / gamma carbonic anhydrase-like 2, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / uncharacterized protein LOC106766640 / uncharacterized protein LOC106767179 / uncharacterized protein LOC106768957 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13-A / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / uncharacterized protein LOC106771273 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial isoform X1 / Mitochondrial-processing peptidase subunit alpha / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-B / Cytochrome b-c1 complex subunit 6 / uncharacterized protein LOC106775330 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / gamma carbonic anhydrase 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B / uncharacterized protein LOC106779486 / Acyl carrier protein / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / Cytochrome c1-2, heme protein, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / NADH-ubiquinone oxidoreductase chain 3 類似検索 - 構成要素生物種 Vigna radiata (リョクトウ)手法 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度 : 3.2 Å 詳細データ登録者 Maldonado, M. / Letts, J.A. 資金援助 米国, 1件 詳細 詳細を隠す組織 認可番号 国 Department of Energy (DOE, United States) DE-SC0022293 米国
引用ジャーナル : Nat Plants / 年 : 2023タイトル : Plant-specific features of respiratory supercomplex I + III from Vigna radiata.著者 : M Maldonado / Z Fan / K M Abe / J A Letts / 要旨 : The last steps of cellular respiration-an essential metabolic process in plants-are carried out by mitochondrial oxidative phosphorylation. This process involves a chain of multi-subunit membrane ... The last steps of cellular respiration-an essential metabolic process in plants-are carried out by mitochondrial oxidative phosphorylation. This process involves a chain of multi-subunit membrane protein complexes (complexes I-V) that form higher-order assemblies called supercomplexes. Although supercomplexes are the most physiologically relevant form of the oxidative phosphorylation complexes, their functions and structures remain mostly unknown. Here we present the cryogenic electron microscopy structure of the supercomplex I + III from Vigna radiata (mung bean). The structure contains the full subunit complement of complex I, including a newly assigned, plant-specific subunit. It also shows differences in the mitochondrial processing peptidase domain of complex III relative to a previously determined supercomplex with complex IV. The supercomplex interface, while reminiscent of that in other organisms, is plant specific, with a major interface involving complex III's mitochondrial processing peptidase domain and no participation of complex I's bridge domain. The complex I structure suggests that the bridge domain sets the angle between the enzyme's two arms, limiting large-scale conformational changes. Moreover, complex I's catalytic loops and its response in active-to-deactive assays suggest that, in V. radiata, the resting complex adopts a non-canonical state and can sample deactive- or open-like conformations even in the presence of substrate. This study widens our understanding of the possible conformations and behaviour of complex I and supercomplex I + III. Further studies of complex I and its supercomplexes in diverse organisms are needed to determine the universal and clade-specific mechanisms of respiration. 履歴 登録 2022年8月23日 登録サイト : RCSB / 処理サイト : RCSB改定 1.0 2023年1月11日 Provider : repository / タイプ : Initial release改定 1.1 2023年2月8日 Group : Database references / カテゴリ : citation / citation_authorItem : _citation.journal_volume / _citation.page_first ... _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.year / _citation_author.identifier_ORCID
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