EMDB-27934: Vigna radiata supercomplex I+III2 (full bridge)

Basic information

Entry

Database: EMDB / ID: EMD-27934

• Title: Vigna radiata supercomplex I+III2 (full bridge)
• Map data: Vigna radiata supercomplex I III2 (SC I III2) composite map of full supercomplex (bridged class 1).

Sample

• Complex: Vigna radiata supercomplex I+III2 (full bridge)
  • Protein or peptide: x 59 types
• Ligand: x 13 types

• Keywords: respiratory supercomplex / nadh-cyt c oxidoreductase / membrane complex / ELECTRON TRANSPORT

Function / homology

Function:

TIM22 mitochondrial import inner membrane insertion complex / mitochondrial processing peptidase / P450-containing electron transport chain / protein insertion into mitochondrial inner membrane / NADH dehydrogenase complex / respiratory chain complex III / quinol-cytochrome-c reductase / quinol-cytochrome-c reductase activity / mitochondrial electron transport, ubiquinol to cytochrome c / NADH dehydrogenase activity / acyl binding / electron transport coupled proton transport / acyl carrier activity / NADH:ubiquinone reductase (H+-translocating) / mitochondrial respiratory chain complex I assembly / mitochondrial electron transport, NADH to ubiquinone / respiratory chain complex I / NADH dehydrogenase (ubiquinone) activity / quinone binding / ATP synthesis coupled electron transport / aerobic respiration / chloroplast / respiratory electron transport chain / electron transport chain / mitochondrial membrane / metalloendopeptidase activity / mitochondrial intermembrane space / 2 iron, 2 sulfur cluster binding / mRNA processing / 4 iron, 4 sulfur cluster binding / response to oxidative stress / electron transfer activity / mitochondrial inner membrane / mRNA binding / heme binding / protein-containing complex binding / mitochondrion / proteolysis / metal ion binding / nucleus / membrane / cytoplasm

Domain/homology:

Mitochondrial import inner membrane translocase subunit TIM22 / NADH-ubiquinone oxidoreductase 11kDa subunit / Putative NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / NADH-ubiquinone oxidoreductase 11 kDa subunit / At2g27730-like / Cytochrome b-c1 complex subunit 8, plants / Cytochrome b-c1 complex subunit 8 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, plant/fungi / : / : / : / Hexapeptide repeat of succinyl-transferase / NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal / NADH-ubiquinone oxidoreductase complex I, 21 kDa subunit / Adrenodoxin / NAD(P)H-quinone oxidoreductase subunit 3, bacterial/plastid / : / Cytochrome b-c1 complex, subunit 6 / Cytochrome b-c1 complex subunit 7 / Cytochrome b-c1 complex subunit 7 superfamily / Ubiquinol-cytochrome C reductase complex 14kD subunit / Cytochrome b-c1 complex subunit 8 superfamily / Cytochrome b-c1 complex subunit Rieske, transmembrane domain / Ubiquinol cytochrome reductase transmembrane region / Cytochrome b-c1 complex subunit 9 / Cytochrome b-c1 complex subunit 9 superfamily / Cytochrome b-c1 complex subunit 9 / Ubiquinol-cytochrome C reductase hinge domain / Ubiquinol-cytochrome C reductase hinge domain superfamily / Ubiquinol-cytochrome C reductase hinge protein / Hexapeptide repeat / Cytochrome c1, transmembrane anchor, C-terminal / : / Ubiquinol-cytochrome c reductase, iron-sulphur subunit / Cytochrome c1 / Cytochrome C1 family / Peptidase M16, zinc-binding site / Insulinase family, zinc-binding region signature. / Tim17/Tim22/Tim23/Pmp24 family / Rieske iron-sulphur protein, C-terminal / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 / Rieske iron-sulphur protein / NADH-ubiquinone oxidoreductase MWFE subunit / : / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex subunit 1 / NADH-ubiquinone oxidoreductase NDSU1/NuoG-like, 4Fe-4S domain / Peptidase M16, C-terminal / Zinc finger, CHCC-type / Peptidase M16 inactive domain / Zinc-finger domain / Peptidase M16, N-terminal / Insulinase (Peptidase family M16) / Metalloenzyme, LuxS/M16 peptidase-like / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 / NADH-ubiquinone oxidoreductase B12 subunit family / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7, NDUB7 / NADH-ubiquinone oxidoreductase B18 subunit (NDUFB7) / GRIM-19 / GRIM-19 protein / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / NDUFB9, LYR domain / NDUFA6, LYR domain / NADH dehydrogenase ubiquinone Fe-S protein 4-like superfamily / NADH dehydrogenase ubiquinone Fe-S protein 4 / Trimeric LpxA-like superfamily / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 2 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 / NADH dehydrogenase ubiquinone Fe-S protein 4, mitochondrial / ETC complex I subunit conserved region / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / NADH ubiquinone oxidoreductase subunit NDUFA12 / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 6 / NADH-quinone oxidoreductase, chain G, C-terminal / NADH-ubiquinone oxidoreductase subunit G, C-terminal / NADH dehydrogenase [ubiquinone] (complex I), alpha subcomplex, subunit 8 / : / NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain / Respiratory-chain NADH dehydrogenase 20 Kd subunit signature. / NADH-ubiquinone oxidoreductase, 20 Kd subunit / NADH-quinone oxidoreductase, chain I / NADH:ubiquinone oxidoreductase, subunit G / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 3. / Rieske [2Fe-2S] domain / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 2. / Rieske [2Fe-2S] iron-sulphur domain / Rieske [2Fe-2S] iron-sulfur domain profile. / Rieske [2Fe-2S] iron-sulphur domain superfamily / Complex 1 LYR protein domain / Complex 1 protein (LYR family) / Respiratory-chain NADH dehydrogenase 75 Kd subunit signature 1. / : / NADH:ubiquinone oxidoreductase, 75kDa subunit, conserved site / NADH:ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH:ubiquinone oxidoreductase, subunit 3 superfamily / NADH-ubiquinone/plastoquinone oxidoreductase, chain 3 / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / NADH-ubiquinone oxidoreductase-G iron-sulfur binding region / 2Fe-2S iron-sulfur cluster binding domain / Respiratory-chain NADH dehydrogenase 24 Kd subunit signature. / NAD-dependent epimerase/dehydratase

Component:

Acyl carrier protein / NADH dehydrogenase [ubiquinone] flavoprotein / Uncharacterized protein / NADH dehydrogenase [ubiquinone] iron-sulfur protein / Cytochrome b-c1 complex subunit Rieske, mitochondrial / Uncharacterized protein LOC106754061 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2 / Outer envelope pore protein 16-3, chloroplastic/mitochondrial / Serine/arginine-rich-splicing factor SR34 isoform X2 / NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial / Complex III subunit 9 / Uncharacterized protein LOC106758628 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 / mitochondrial processing peptidase / Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 / Uncharacterized protein LOC106761134 / Gamma carbonic anhydrase 1, mitochondrial-like / NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial / Cytochrome b-c1 complex subunit 7 / Cytochrome b-c1 complex subunit 8 / Complex III subunit II / Gamma carbonic anhydrase-like 2, mitochondrial / NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial / Uncharacterized protein LOC106766640 / Uncharacterized protein LOC106767179 / Uncharacterized protein LOC106768957 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 / Uncharacterized protein LOC106771273 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial isoform X1 / Complex III subunit II / NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-B / Cytochrome b-c1 complex subunit 6 / Uncharacterized protein LOC106775330 / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 / Gamma carbonic anhydrase 1, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B / Uncharacterized protein LOC106779486 / Acyl carrier protein / NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial / Cytochrome c1-2, heme protein, mitochondrial / NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 / NADH-ubiquinone oxidoreductase chain 3

• Biological species: Vigna radiata (mung bean)
• Method: single particle reconstruction / cryo EM / Resolution: 3.2 Å
• Authors: Maldonado M / Letts JA

Funding support

United States, 1 items

Organization	Grant number	Country
Department of Energy (DOE, United States)	DE-SC0022293	United States

• Citation: Journal: Nat Plants / Year: 2023; Title: Plant-specific features of respiratory supercomplex I + III from Vigna radiata.; Authors: M Maldonado / Z Fan / K M Abe / J A Letts / ; Abstract: The last steps of cellular respiration-an essential metabolic process in plants-are carried out by mitochondrial oxidative phosphorylation. This process involves a chain of multi-subunit membrane protein complexes (complexes I-V) that form higher-order assemblies called supercomplexes. Although supercomplexes are the most physiologically relevant form of the oxidative phosphorylation complexes, their functions and structures remain mostly unknown. Here we present the cryogenic electron microscopy structure of the supercomplex I + III from Vigna radiata (mung bean). The structure contains the full subunit complement of complex I, including a newly assigned, plant-specific subunit. It also shows differences in the mitochondrial processing peptidase domain of complex III relative to a previously determined supercomplex with complex IV. The supercomplex interface, while reminiscent of that in other organisms, is plant specific, with a major interface involving complex III's mitochondrial processing peptidase domain and no participation of complex I's bridge domain. The complex I structure suggests that the bridge domain sets the angle between the enzyme's two arms, limiting large-scale conformational changes. Moreover, complex I's catalytic loops and its response in active-to-deactive assays suggest that, in V. radiata, the resting complex adopts a non-canonical state and can sample deactive- or open-like conformations even in the presence of substrate. This study widens our understanding of the possible conformations and behaviour of complex I and supercomplex I + III. Further studies of complex I and its supercomplexes in diverse organisms are needed to determine the universal and clade-specific mechanisms of respiration.

History

Deposition	Aug 23, 2022	-
Header (metadata) release	Jan 11, 2023	-
Map release	Jan 11, 2023	-
Update	Dec 25, 2024	-
Current status	Dec 25, 2024	Processing site: RCSB / Status: Released

Map

• File: Download / File: emd_27934.map.gz / Format: CCP4 / Size: 87.2 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
• Annotation: Vigna radiata supercomplex I III2 (SC I III2) composite map of full supercomplex (bridged class 1).
• Voxel size: X=Y=Z: 0.88 Å

Density

Contour Level	By AUTHOR: 6.5
Minimum - Maximum	-25.093775000000001 - 59.066498000000003
Average (Standard dev.)	0.28915003 (±2.8661964)

• Symmetry: Space group: 1

Details

EMDB XML:

Map geometry	Axis order Z Y X Origin 180 133 179 Dimensions 269 376 226 Spacing 226 269 376
Cell	A: 198.88 Å / B: 236.72 Å / C: 330.88 Å α=β=γ: 90.0 °

Supplemental data

Mask #1

• File: emd_27934_msk_1.map

Additional map: Vigna radiata supercomplex I III2 bridged class 1

• File: emd_27934_additional_1.map
• Annotation: Vigna radiata supercomplex I III2 bridged class 1

Half map: Vigna radiata supercomplex I III2 bridged class 1 half map B

• File: emd_27934_half_map_1.map
• Annotation: Vigna radiata supercomplex I III2 bridged class 1_half map B

Half map: Vigna radiata supercomplex I III2 bridged class 1 half map A

• File: emd_27934_half_map_2.map
• Annotation: Vigna radiata supercomplex I III2 bridged class 1_half map A

Sample components

Entire : Vigna radiata supercomplex I+III2 (full bridge)

• Entire: Name: Vigna radiata supercomplex I+III2 (full bridge)

Components

• Complex: Vigna radiata supercomplex I+III2 (full bridge)
  • Protein or peptide: MPP-beta
  • Protein or peptide: MPP-alpha (protomer 1)
  • Protein or peptide: COB (cyt b)
  • Protein or peptide: CYC1 (cyt c1)
  • Protein or peptide: UCR1 (Rieske iron-sulfur protein subunit)
  • Protein or peptide: QCR7
  • Protein or peptide: QCR8
  • Protein or peptide: QCR6
  • Protein or peptide: QCR9
  • Protein or peptide: QCR10 (UCRY)
  • Protein or peptide: MPP-alpha (protomer 2)
  • Protein or peptide: Nad1
  • Protein or peptide: Nad2
  • Protein or peptide: Nad3
  • Protein or peptide: Nad4
  • Protein or peptide: Nad4L
  • Protein or peptide: Nad5
  • Protein or peptide: Nad6
  • Protein or peptide: NDUA1
  • Protein or peptide: NDUA2
  • Protein or peptide: NDUA3
  • Protein or peptide: NDUA5
  • Protein or peptide: NDUA6
  • Protein or peptide: NDUA7
  • Protein or peptide: NDUA8
  • Protein or peptide: NDUA9
  • Protein or peptide: NDUA11
  • Protein or peptide: NDUA12
  • Protein or peptide: NDUA13
  • Protein or peptide: NDUAB1-beta
  • Protein or peptide: NDUAB1-alpha
  • Protein or peptide: NDUB2
  • Protein or peptide: NDUB3
  • Protein or peptide: NDUB4
  • Protein or peptide: NDUB7
  • Protein or peptide: NDUB8
  • Protein or peptide: NDUB9
  • Protein or peptide: NDUB10
  • Protein or peptide: NDUB11
  • Protein or peptide: NDUFX
  • Protein or peptide: NDUC2
  • Protein or peptide: NDUP2
  • Protein or peptide: NDUCA1
  • Protein or peptide: NDUCA2
  • Protein or peptide: NDUCAL2
  • Protein or peptide: NDUS1
  • Protein or peptide: NDUS2
  • Protein or peptide: NDUS3
  • Protein or peptide: NDUS4
  • Protein or peptide: NDUS5
  • Protein or peptide: NDUS6
  • Protein or peptide: NDUS7
  • Protein or peptide: NDUS8
  • Protein or peptide: NDUP1
  • Protein or peptide: NDUP4
  • Protein or peptide: NDUB6
  • Protein or peptide: NDUV1
  • Protein or peptide: NDUV2
  • Protein or peptide: NDUX1
• Ligand: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE
• Ligand: CARDIOLIPIN
• Ligand: PROTOPORPHYRIN IX CONTAINING FE
• Ligand: 1,2-Distearoyl-sn-glycerophosphoethanolamine
• Ligand: HEME C
• Ligand: UBIQUINONE-10
• Ligand: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
• Ligand: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate
• Ligand: FE (III) ION
• Ligand: ZINC ION
• Ligand: IRON/SULFUR CLUSTER
• Ligand: FE2/S2 (INORGANIC) CLUSTER
• Ligand: FLAVIN MONONUCLEOTIDE

Supramolecule #1: Vigna radiata supercomplex I+III2 (full bridge)

• Supramolecule: Name: Vigna radiata supercomplex I+III2 (full bridge) / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#59; Details: Higher-order assembly between respiratory complex I and complex III2
• Source (natural): Organism: Vigna radiata (mung bean)

Macromolecule #1: MPP-beta

• Macromolecule: Name: MPP-beta / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 58.711238 KDa

Sequence

String:

MSLNRLLSAA RRSDRRASAL SSFRSLSASP ALAPSAASPP APPPPTAMIY DRAAEAIKSK LRQLENPDPR FLKHGNPRPA LSDHTRILA APETRVTTLP NGLRVATESS LAARTATVGV WIDAGSRFET EETNGTAHFL EHMIFKGTER RNARELEEEI E NMGGHLNA YTSREQTTYY AKVTDSDVPQ ALDILADILQ NSRFEENRIS RERDVILREM EEVEGQTEEV IFDHLHATAF QY TPLGRTI LGPAQNIKTI TKAHLQSYIQ THYTAPRMVI AASGAVKHED IVEQVKKLFT KLSTDATTTA QLVAKEPAIF TGS EVRMLD DDIPLAQFAV AFEGASWKDP DSIALMVMQA MLGSWNKTAG GGKHMGSELA QRVGINEVAE SIMAFNTNYK DTGL FGVYA VAKPDCLDDL SYAIMQETTK LVYRVSEDDV TRACNQLKSS LLLHIDGTSP VAEDIGRQLL TYGRRIPFAE LFARI DAVD ASTIKRVANR FIYDKDVAIA AMGPIQRLPD YNWFRRRTYW NRY

UniProtKB: mitochondrial processing peptidase

Macromolecule #2: MPP-alpha (protomer 1)

• Macromolecule: Name: MPP-alpha (protomer 1) / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 55.244543 KDa

Sequence

String:

MYRNVASRLR TIRARSCSRL PVRFASSSSS SVATKQSSSG LGGLFGWLTG DRSSSATPLD FPLPGVILSP PLPDYVAPGK TIITTLPNG VKVASETSAT PTASIGLYVD CGSIYESPIS FGATHLLERM AFKTTRNRSH FRVVREVEAI GGNVQASASR E QMGYTFDA LKTYVPEMVE LLVDCVRNPA FLDWEVNEQL LKVKAEIGEA SKNPQDLLLE AIHSAGFSGA LANPLLASES AI NRLNGAI LEEFVAENYT APRIVLAASG VEHEELLSVA EPLLSDLPSV PRPEEPKSVY TGGDYRCQSE TGRTHFALAF ELP GGWHKL KDAMVLTVLQ MLLGGGGSFS AGGPGKGMYS RLYLNVLNQY PQFHSISAFN NIYNDTGIFG IQVTTSSDFI SKAI DITAN EILAVATHGK VEQLQLDRAK QATKSAILMN LESRMVVSED IGRQILTYGE RKPVEEFLKA VDEVTLKDIS SISQK LISS PLTMASYGDV LYVPSYESVS SKFPTK

UniProtKB: Complex III subunit II

Macromolecule #3: COB (cyt b)

• Macromolecule: Name: COB (cyt b) / type: protein_or_peptide / ID: 3 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 44.454645 KDa

Sequence

String:

MTIRNQRFSL LKEPISSTLN QHLIDYPTPS NLSYWWGFGS LAGICLVIQI VTGVFLAMHY TPHVDLAFNS VEHVMRDVEG GWLLRYMHA NGASMFFIVV YLHIFRGLYY ASYSSPREFV WCLGVVIFLL MIVTAFTGYV LPWGQMSFWG ATVITSLASA I PVVGDTIV TWLWGGFSVD NATLNRFFSL HYLLPFLLVG ASLLHLAALH QYGSNNPLGV HSEMDQISFY PYFYVKDLVG WV AFAIFFS IWIFYAPNVL GHPDNYIPAN PMSTPPHIVP EWYFLPIYAI LRSIPDKSGG VAAIALVFIC LLALPFFKSM YVR SSSFRP IYQGIFWLLL ADCLLLGWIG CQPVEAPFVT IGQISSFVFF LFFAITPILG RVGRGIPNSY TTDETEMT

Macromolecule #4: CYC1 (cyt c1)

• Macromolecule: Name: CYC1 (cyt c1) / type: protein_or_peptide / ID: 4 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 33.556188 KDa

Sequence

String:

MTGGVFQLLR RKLHPQFTNS SLLSPIIAKK DGAGSTGSRS LKVLALIGAG VSGFFSFSTV AVADEAEHGL ACPSYPWPHK GILSSYDHA SIRRGHQVYT EVCASCHSMS LISYRDLVGV AYTEEEVKAM AAEIEVVDGP NDEGEMFTRP GKLSDRFPQP Y ANEAAARF ANGGAYPPDL SLITKARHNG QNYVFSLLTG YRDPPAGVSI REGLHYNPYF PGGAIAMPKM LNDGAVEYED GT PATEAQM GKDVVSFLSW AAEPEMEERK LMGFKWIFVL SLALLQAAYY RRLRWSVLKS RKLVLDVVN

UniProtKB: Cytochrome c1-2, heme protein, mitochondrial

Macromolecule #5: UCR1 (Rieske iron-sulfur protein subunit)

• Macromolecule: Name: UCR1 (Rieske iron-sulfur protein subunit) / type: protein_or_peptide / ID: 5 / Number of copies: 2 / Enantiomer: LEVO / EC number: quinol-cytochrome-c reductase
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 30.051432 KDa

Sequence

String:

MLRVAAKRLS SLSSSAWRAN HAASAVLSRN PVAPSLSTEE HRSDPFSLRP EFFLPIRGYA TDSLFHPKEN SLIPEIPATV AAVKNPSSK IIYDEHNHER FPPGDPSKRA FAYFVLTGGR FVYASLIRLL VLKFVLSMSA SKDVLALASL EVDLSSIEPG T TVTVKWRG KPVFIRRRTE DDIKLANSVD VASLRDPQQD AERVKNPEWL IVIGVCTHLG CIPLPNAGDF GGWFCPCHGS HY DISGRIR KGPAPYNLEV PTYTFLEENK LMIG

UniProtKB: Cytochrome b-c1 complex subunit Rieske, mitochondrial

Macromolecule #6: QCR7

• Macromolecule: Name: QCR7 / type: protein_or_peptide / ID: 6 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 14.392698 KDa

Sequence

String:

MASFLQSFLD PKKNWLAAQH MKSLVKRLSK YGLRYDDLYD PYYDLDVKEA LNRLPKEVVD ARHARLKRAI DLSMKHEYLP EDLQAMQTP FRGYLQDMLA LVKRERAERE ALGGLPLYQR SIP

UniProtKB: Cytochrome b-c1 complex subunit 7

Macromolecule #7: QCR8

• Macromolecule: Name: QCR8 / type: protein_or_peptide / ID: 7 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 8.349831 KDa

Sequence

String:

MGKQIVPVKS VIYALSPFQQ KVMTGLWKDL PTKIHHKVSE NWISATLLLG PLVGTYAYVQ NYLEKEKLHH RY

UniProtKB: Cytochrome b-c1 complex subunit 8

Macromolecule #8: QCR6

• Macromolecule: Name: QCR6 / type: protein_or_peptide / ID: 8 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 8.117424 KDa

Sequence

String:

MADEEPVDQK RYLEESCKPK CVKPLLEYQA CIKRIHGDDS GQKHCTGQYF DYWFCVDKCV APKLFTKLK

UniProtKB: Cytochrome b-c1 complex subunit 6

Macromolecule #9: QCR9

• Macromolecule: Name: QCR9 / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 8.20342 KDa

Sequence

String:

METVARRRGG GIFESLYKVV MRRNSVYVTF VIAGAFLGER AVDYGIHKLW EANNVGKRYE DIPVLGQKLS EE

UniProtKB: Complex III subunit 9

Macromolecule #10: QCR10 (UCRY)

• Macromolecule: Name: QCR10 (UCRY) / type: protein_or_peptide / ID: 10 / Number of copies: 2 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 8.948875 KDa

Sequence

String:

MAGLPARLRI QPADVKAAAM WGVAAATGGL YLVQVSILVL PPVKVVFHFY LVSGFRICLD VKDLRTMPCS APRIWRLYIL I

Macromolecule #11: MPP-alpha (protomer 2)

• Macromolecule: Name: MPP-alpha (protomer 2) / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 54.537684 KDa

Sequence

String:

MYRAAASSFR RHLKGHGSKL GSTRSSTSAA VAARTSKGGL FSWLTGERSS SLPSLDIPLG GVVLPDPLPD SVEQSKTKIT TLSNGLKIA SETSPNPAAS IGLYLDCGSI YETPFSSGAS HLLERMAFKS TTNRSHFRIV REVEAIGGNI GASASREQMG Y TFDALKTY VPQMVELLVD CVRNPAFLDW EVNEELRKVK AELGELSNNP QGLLLEAIHS AGYSGALAYP LLAPEAALNR LD GPSLEEF VAENYTAPRM VLAAAGVEHE ELVSIAEPLL SDLPNVPRPD EPKSVYVGGD FRRHGESGGT HVALAFEVPG GWH KEKDAI VLTVLQMLMG GGGSFSAGGP GKGMHSRLYL RVLNEYQQIQ SFSAFNSIFN NTGLFGIYAS TSPDFAPKAV DIAA KELIA IASPGQVTQV QLDRAKKSTK SAVLMNLESR MIASEDIGRQ ILTYGERKPL EQFLKAVDEI TLNDITKISQ KIISS PLTM ASYGDVLSVP SYESVNRKFH AK

UniProtKB: Complex III subunit II

Macromolecule #12: Nad1

• Macromolecule: Name: Nad1 / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 35.771562 KDa

Sequence

String:

MYIAVPAEIL GIILPLLLGV AFLVLAERKV MAFVQRRKGP DVVGSFGLLQ PLADGLKLIL KEPISPSSAN FFLFRMAPVA TFMLSLVAW AVVPFDYGMV LSDSNIGLLY LFAISSLGVY GIITAGWSSN SKYAFLGALR SAAQMVSYEV SIGLILITVL I CVGSCNLS EIVMAQKQIW FGIPLFPVLV MFFISCLAET NRAPFDLPEA EAESVAGYNV EYSSMGFALF FLGEYANMIL MS GLCTSLS LGGWLPILDL PIFKRIPGSI WFSIKVILFL FLYIWVRAAF PRYRYDQLMG LGWKVFLPLS LAWVVAVSGV SVT FPWLP

Macromolecule #13: Nad2

• Macromolecule: Name: Nad2 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 54.06302 KDa

Sequence

String:

MFNLFLAVYP EIFIINATFI LLIHGVVFST SKKYDYPPLV SNVGWLGLLS VLITLLLLAA GAPLLTIAHL FRNNFFRRDN FTYFCQILL LLSTAGTISM CFDFFEQERF DAFEFIVLIL LSTCSMLFMI SAYDLIAMYL AIELQSLCFY VMAASKRKSE F STEAGLKY LILGAFSSGI LLFGCSMIYG STGATHFDQL AKILTGYELA GARSSGIFMG ILFIAVGSLF KITAVPFHMW AP DIYEGSP TPVTAFLSIA PKISIFANIL RVFIYGSYGA TLQQIFCFCS IASMILGALA AMAQTKVKRL LAYSSIGHVG YIC IGFSCG TIEGIQSLLI GIFIYALMTI DAFAIVLALR QTRVKYIADL GALAKTNPIL AITFSITMFS YVGIPPLAGF CSKF YLFFA ALGCGAYFLA SVGVVTSVIG CFYYIRLVKR MFFDTPRTWI LYEPMDRDKS LLLAMTSSFI TLFFLYPSPL FSVTH QMAL SLYL

Macromolecule #14: Nad3

• Macromolecule: Name: Nad3 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 13.746171 KDa

Sequence

String:

MLEFAPICIY LVISLLVSLI LLGLPFLFAS NSSTYPEKLS AYECGFDPFG DARSRFDIRF YLVSILFIIF DLEVTFFFPW AVSLNKIDL FGFWSMMAFL LILTIGFLYE WKRGALDWE

UniProtKB: NADH-ubiquinone oxidoreductase chain 3

Macromolecule #15: Nad4

• Macromolecule: Name: Nad4 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 55.823391 KDa

Sequence

String:

MLEHFCECYF DLSGLILCPV LGSIILIFIP NSRIRLIRLI GLCASLITFL YSLVLWIQFD PSTAKFQFVE SLRWLPYENI NLNLGIDGL SFFFVILTTF LIPICILVGW SGMRSYGKEY IIAFLICEFL MIAVFCMLDL LLFYVFFESV LIPMFIIIGV W GSRQRKIK AAYQFFLYTL LGSVFMLLAI LLILFQTGTA DLQISLTTEF SERRQIFLWI AFFAAFAVKV PMVPVHIWLP EA HVEAPTA GSVILAGILS KLGTYGFLRF SIPMFPEATL CFTPFIYTLS AIAIIYTSLT TLRQIDLKKI IAYSSVAHMN LVT IGMFSL NIQGIGGSIL LMLSHGLVLS ALFLCVGVLY DRHKTRLVRY YGGLVSTMPN FSTIFFFFTL ANMSLPGTSS FIGE FLISV GAFQRNSLVA TLAALGMILG AAYSLWLYNR VVSGNLKADF LHKFSDLNGR EVFIFIPFLV GVVRMGVHPK VFLDR MHTY VSNLVQHGKF N

Macromolecule #16: Nad4L

• Macromolecule: Name: Nad4L / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 11.153495 KDa

Sequence

String:

MDLIKYFTFS MIIFILGIWG ILLNRRNILI MLMSIELMLL AVNSNFLVFS VSLDDMMGQL FALLVLTVAA AESAIGLAIF VITFRVRGT IAVEFINSIQ G

Macromolecule #17: Nad5

• Macromolecule: Name: Nad5 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 74.895273 KDa

Sequence

String:

MYLLIVFLPL IGSSFAGFFG RFLGSEGSAI MTTTCVSFSS ILSLIAFYEV ALGASACYLR IAPWISSEMF DASWGFLFDS LTVVMLIVV TFISSLVHLY SISYMSEDPH SPRFMCYLSI LTFFMLMLVT GDNFLQLFLG WEGVGLASYL LIHFWFTRLQ A DKAATKAM LVNRVGDFGL ALGILGCFTL FQTVDFSTIF ACASAPRNSW IRCNMRLNAI TLICILLFIG AVGKSAQIGS HT WLPDAME GPTPVSALIH AATMVTAGVF MIARCSPLFE YSPTALIVIT FAGAMTSFLA ATTGILQNDL KRVIAYSTCS QLG YMIFAC GISNYSVSVF HLMNHAFFKA LLFLSAGSVI HAMSDEQDMR KMGGLASSFP FTYAMMLMGS LSLIGFPFLT GFYS KDVIL ELAYTKYTIS GNFAFWLGSV SVLFTSYYSF RSLFLTFLVP TNSFGRDILR CHDAPIPMAI PLILLALGSL FVGYL AKDM MIGLGTNFWA NSLFVLPKNE ILAESEFAAP TITKLIPILF STSGASVAYN VNLVADQFQR AFQTSTFCNR LYSFFN KRW FFDQVLNDFL VRSFLRFGYE VSFEALDKGA IEILGPYGIS YTFRRLAERI SQLQSGFVYH YAFAMLLGLT LFVTFFC MW DSLSSWVDNR SSFILIVSSF FNNKSIIINK N

Macromolecule #18: Nad6

• Macromolecule: Name: Nad6 / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 23.604369 KDa

Sequence

String:

MILSVLFSLA LVSGLMVVRA KNPVHSVLFF ILVFCDTSGL LLLLGLDFFA MIFLVVYIGA IAVLFLFVVM MFHIQIAEIH EEVLRYLPV SGIIGLILWW EMFFILDNET IPLLPTQINT TSLIYTVYAG KVRSWTNLET LGNLLYTYYF VWFLVPSLIL L VAMIGAIV LTMHRTTKVK RQDVFRRNAI DFRRTIMRRT TDPLTID

Macromolecule #19: NDUA1

• Macromolecule: Name: NDUA1 / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 7.357572 KDa

Sequence

String:

MNLRWMEAVL PLGIIAGMLC VMGNAQYYIH KAAHGRPKHI GNDLWDVAME RRDKKLHEQA ASSSN

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1

Macromolecule #20: NDUA2

• Macromolecule: Name: NDUA2 / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 10.884632 KDa

Sequence

String:

MAWRGQLSKN IKELRFLMCQ SSSASASARA FVEKNYKELK TLNPKLPILI RECSGVEPQL WARYDLGVEK AIKLEGLSEA QISKALEDL AKAGQSSKA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2

Macromolecule #21: NDUA3

• Macromolecule: Name: NDUA3 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 6.695044 KDa

Sequence

String:

MAVMEKLKMF VVQEPVVAAS CLIAGFGLFL PAVVRPILDS YQATKQPPQP ALSDVVAGMT GKK

UniProtKB: Uncharacterized protein LOC106754061

Macromolecule #22: NDUA5

• Macromolecule: Name: NDUA5 / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 18.977604 KDa

Sequence

String:

MFLRAVARPL MAKVKASTGI VGLEVVPNAR EVLIGLYSKT LKEIQKVPED EGYRKAVESF TKHRLSVCKE EEDWEAIEKR LGCGQVEEL IEEAQDELKL ISYMIEWDPW GVPDDYECEV IENDAPVPKH VPLHRPPPLP TEFHKTLEAL SSQSGKDIPG A SSTESPSK T

UniProtKB: Probable NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5, mitochondrial

Macromolecule #23: NDUA6

• Macromolecule: Name: NDUA6 / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 14.920034 KDa

Sequence

String:

MASALRNVKV LPNSASVEEA RQRVFDFFRA TCRSLPSVME IYNLYDVTSV AQLRSTVASE IRKNIGVSDP KVIDMLLFKG MEELMNVVN HSKQRHHIIG QYVVGQQGQV QDSASKDPAI SPFLKNFYSS NYF

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6

Macromolecule #24: NDUA7

• Macromolecule: Name: NDUA7 / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 14.356376 KDa

Sequence

String:

MAKSASNSLV QTLKRYIKKP WEITGPCADP EYRSAVPLAT EYRLQCPATT KEKPCIPNSL PETVYDIKYF SRDQRRNRPP IRRTVLKKA DVEKLAKEQT FAVSDFPPVY LNSAVEEDIN AIGGGYQG

UniProtKB: Uncharacterized protein LOC106768957

Macromolecule #25: NDUA8

• Macromolecule: Name: NDUA8 / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 12.022893 KDa

Sequence

String:

MASAVDASGN PIPSSSVLMA SSKHIGIRCH SENLDFLKCK KKDPNPEKCL DKGRDVTRCV LGLLKDLHQK CTKEMDDYVG CMYYHTNEF DLCRKEQQAF EKKCSLE

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8-B

Macromolecule #26: NDUA9

• Macromolecule: Name: NDUA9 / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 43.781504 KDa

Sequence

String:

MQTIARRLGH QSLKSSTSIR AIYPISDHHY VADHQRYVST IATKGVGHLV RKGTGGRSSV SGIIATVFGA TGFLGRYVVQ QLAKMGSQV LVPFRGSEDC PRHLKLMGDL GQIVPMKYNP RDESSVKAVM AKANVVINLI GRDYETRNYS FEEVHYHMAE N LAKISREH GGILRFIQVS CLGASPSSPS RMLRAKAAAE EVVLRELPEA TILKPAVMIG TEDRILNPWA HFAKKYGFIP LF GNGSTKI QPVYVVDVAA ALTTVLKDDG TSMGKTYELG GPEIFTVHDL AELMYETIRE WPRYVKVPFP IAKALATPRE ILL NKVPFP LPTPEILNLD KIQALTTDTI VSENALTFND LGIIPHKLKG YPVEFLISYR KGGPQFGSTI SERVTPDAWP

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial isoform X1

Macromolecule #27: NDUA11

• Macromolecule: Name: NDUA11 / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 17.284715 KDa

Sequence

String:

MVDAAERRYL EDEDTPFMKT IKGATTGLVA GTIWGTVVAT WYDVPRVERS VALPGLIRTF KMMGNYGLTF AAIGGVYIGV EQLLQNYRM KRDLVNGAVG GFVAGATILG YKGRSIKTAL SAGSALAFTS AYIDFGGQTL KHDEGKEYAA YTTKKRTSGD E

UniProtKB: Outer envelope pore protein 16-3, chloroplastic/mitochondrial

Macromolecule #28: NDUA12

• Macromolecule: Name: NDUA12 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 18.192482 KDa

Sequence

String:

MASVVKNVLK SIREKGFGTF LRELKDEGYL RCLPDGNLLQ TKIHNIGATL VGVDKFGNKY YEKLGDTQYG RHRWVEYAEK SRYNASQVP PEWHGWLHFI TDHTGDELLL LKPKRYGVEH KENLSGEGEQ YIYHSKGHAL NPGQRDWTRY QPWESKA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12

Macromolecule #29: NDUA13

• Macromolecule: Name: NDUA13 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 16.1896 KDa

Sequence

String:

MTEAYIRNKP GMVSVKDMPV LQDGPPPGGF APVRFARRIP NKGPSAVAIF LAAFGTFSWG MYQVGQGNKI RRALKEEKYA ARRSILPVL QAEEDERFVR EWHKYLAYEA EVMKDVPGWK VGESVYNSGR WVPPASGELR PDIW

UniProtKB: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13

Macromolecule #30: NDUAB1-beta

• Macromolecule: Name: NDUAB1-beta / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 13.066032 KDa

Sequence

String:

MAVRAAVLRH IRVPLQAVPR LQPWRAMSSH GDDHLSKEEV IQRVLAVVKD FPKVDPSKVT PDVHFQKDLG LDSLDNVEIV MALEEEFKL EIPDKEADKI DSCNLAIEYI ANHPMAG

UniProtKB: Acyl carrier protein

Macromolecule #31: NDUAB1-alpha

• Macromolecule: Name: NDUAB1-alpha / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 14.314356 KDa

Sequence

String:

MAASRSTLPL IKYLRVRVDA LPRNSPLTLA PTVFLRRFCE EVRGSFLDKS EVADRVISCV KNFQKVDPAK VTPNAHFQND LGLDSLDAV EIVMALEEEF GFEIPDNEAD KINSIKHAVD FIASHPQAK

UniProtKB: Acyl carrier protein

Macromolecule #32: NDUB2

• Macromolecule: Name: NDUB2 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 7.555608 KDa

Sequence

String:

MGGHGEGTTY KGVTIHQPKR WHTITGKGLC AVMWFWVLYR AKQDGPVVLG WRHPWEGHHD DHGKGH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2

Macromolecule #33: NDUB3

• Macromolecule: Name: NDUB3 / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 7.843885 KDa

Sequence

String:

MGSGKHTAEF FRRRDAWRKH PMLTNQFRHA TPGLGIALVA FGFYLVGEQV YDRLNADSHV HVKAENHH

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3-B

Macromolecule #34: NDUB4

• Macromolecule: Name: NDUB4 / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 8.364627 KDa

Sequence

String:

MGGGMEANKN KFIEDWGTAR ENLEFNFRWT RRNLALVGIF GIAIPVLVYK GIVREFHMQD EDNGRPYRKF M

UniProtKB: Uncharacterized protein LOC106766640

Macromolecule #35: NDUB7

• Macromolecule: Name: NDUB7 / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 11.600554 KDa

Sequence

String:

MEVEGSSKKM IATQEEMVEA RVPLAYRDQC AHLLIPLNKC RQAEFYLPWK CENERHSYEK CEYELVMERM LQMQKIREEQ KKADAKQPL ILVPKPANA

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7

Macromolecule #36: NDUB8

• Macromolecule: Name: NDUB8 / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 13.592655 KDa

Sequence

String:

MAGRLTNAAS RILGGNGVVY RSVASSLRLR SGMGLPVGKH YIPDKPLPMN EELLWDNGTP FPEPCIDRIA DTVGKYEALA WLCGGLSFF ASLGLLAVWN DKASKIPFTP KVYPYDNLRV ELGGEP

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial

Macromolecule #37: NDUB9

• Macromolecule: Name: NDUB9 / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 13.519021 KDa

Sequence

String:

MASTAAYLAR RAAQKERVRI LYRRALKDTL NWAVHRHLFY NDAENLRDRF EENKHVEDPD TIDRLIADAE ASYNKWRHPD PYIVPWAPG GSKFTRNPAP PQGIEIVYDY GREDNN

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9

Macromolecule #38: NDUB10

• Macromolecule: Name: NDUB10 / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 12.587426 KDa

Sequence

String:

MGRKKGYVEF DESPPDDFDP ANPYKDPVAM LEMREYIVRE KWIQIEKAKI IREKLRWCYR IEGVNHLQKC RHLVNQYLES TRGIGWGKD GRHPSLHAPK VEPVESE

UniProtKB: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10-B

Macromolecule #39: NDUB11

• Macromolecule: Name: NDUB11 / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 13.157823 KDa

Sequence

String:

MPPATPFATA SATIRRRVLQ SLRTRGGAES GPSRWTSPGH EERPKGYLFN RTPPPPGQSR KWEDWELPCY ITSFLTIVIL GVGLNAKPD LTIETWAHEK ALERLKIENA LASNNDSSE

UniProtKB: Uncharacterized protein LOC106771273

Macromolecule #40: NDUFX

• Macromolecule: Name: NDUFX / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 17.610127 KDa

Sequence

String:

MAVANLQRLS SQVQRLPSLS FFSKSLISRS TATSSSSKKV SDRIVRLSAI DFEGKKHEVV GLAGHTLLKA LINTGLIDPD SHRLEEIDA CSAHCEVNIA QEWLDKLPPR SYDEEYVLVR NSRARVLNKH SRLGCQVLLD HNLQGMVVAL PEPKPWDTS

UniProtKB: Uncharacterized protein LOC106779486

Macromolecule #41: NDUC2

• Macromolecule: Name: NDUC2 / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 9.243669 KDa

Sequence

String:

MVLSATTIGA LLGLGTQMYS NALRKLPYMR HPWEHVVGMG LGAVFVNQLL KWEAQVEQDL DKMLEKAKAA NERRYIDGDD DI

Macromolecule #42: NDUP2

• Macromolecule: Name: NDUP2 / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 12.172666 KDa

Sequence

String:

MAARVAARYG SRRLFSSGSG KILSEEEKAA ENAYFKKAEQ DKLEKLARKG PQPEASSGGS VIDAKPSGSG HTGASAERVS TDKHRNYAV VAGTITILGA LGWYLKGTAK KPEVQD

UniProtKB: Uncharacterized protein

Macromolecule #43: NDUCA1

• Macromolecule: Name: NDUCA1 / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 29.333197 KDa

Sequence

String:

MGTLGRAFYA VGFWIRETGQ AIDRLGSRLQ GNYLFQEQLS RHRPLMNLFD KYPSVHKDAF VAPSASLLGD VHVGPASSIW YGCVLRGDV NGIVIGSGTN IQDNSLVHVA KSNLTGKVLP TIIGDNVTVG HSAVLQGCTV EDEAFIGMGA TLLDGVYVEK H AMVAAGAL VRQNTRIPYG EVWGGNPAKF LRKLTEDEMA FFSQSALNYS NLAQAHAAEN AKELEETEFV KVLHKKFAPR GE EYDSILD GGQETPAKLN LQDNVLLDKA PKA

UniProtKB: Gamma carbonic anhydrase 1, mitochondrial

Macromolecule #44: NDUCA2

• Macromolecule: Name: NDUCA2 / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 29.780861 KDa

Sequence

String:

MGTLGRAIYS VGFWIRETGQ AIDRLGSRLQ GGYFFQEQLS RHRTLMNIFD KAPVVDKDVF VAPSASVIGD VQVGRGSSIW YGCVLRGDV NSIRVGSGTN IQDNSLVHVA KSNLSGKVLP TVIGDNVTVG HSAVLHGCTV EDEAFVGMGA VLLDGVVVEK N AMVAAGAL VRQNTRIPSG EVWAGNPAKF LRKLSNEEIT FISQSAINYT NLAQVHAAEN SKSYDEIEFE KVLRKKYARK DE EYDSMLG VVREIPPELI LPDNVLPDKA EKALQK

UniProtKB: Gamma carbonic anhydrase 1, mitochondrial-like

Macromolecule #45: NDUCAL2

• Macromolecule: Name: NDUCAL2 / type: protein_or_peptide / ID: 45 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 28.268359 KDa

Sequence

String:

MANLARFSKR ALRSAHSLVR HAQSQVQPQL FAEERAFSTE AAKSITPSPD RVKWDYRGQR KIIPLGQWLP KVAVDAYVAP NVVLAGQVT VWDGASVWPG CVLRGDLNKI SIGFCSNVQE RSVLHAAWTS PTGLPADTSI ERYVTIGAYS LLRSCTIEPE C IIGQHSIL MEGSLVETQS ILEAGSVVPP GRRIPSGELW AGNPARFVRT LTHEEILEIP KLAVAINDLS RDHYSEFLPY ST VYLEVEK FKKSLGISV

UniProtKB: Gamma carbonic anhydrase-like 2, mitochondrial

Macromolecule #46: NDUS1

• Macromolecule: Name: NDUS1 / type: protein_or_peptide / ID: 46 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 81.434219 KDa

Sequence

String:

MGFGLLASKA LRPTSRLLLS SQNPTIFLRT IVSKPQLCNP EASAAQPAQP PPVDLPPRSP LAGARVHFAN PDDAIEVFVD GYPVKIPKG MTVLQACEVA GVDIPRFCYH SRLSIAGNCR MCLVEVEKSP KPVASCAMPA LPGMKIKTDT PVAKKAREGV M EFLLMNHP LDCPICDQGG ECDLQDQSMA FGSDRGRFTE VKRSVVDKNL GPLVKTVMTR CIQCTRCVRF ATEVAGVQDL GM LGRGSGE EIGTYVEKLL TSELSGNVID ICPVGALTSK PFAFKARNWE LKGTETIDVT DAVGSNIRID SRGPEVMRIV PRL NEDINE EWISDKTRFC YDGLKRQRLN DPMIRGPDGR FKAVNWRDAL SVIADIAHQV KPEEIVGVAG KLSDAESMIA LKDF LNRMG SNDVWGEGIG VNTNADFRSG YIMNTSIAGL EKADVFLLVG TQPRVEAAMV NARIRKTVRS NQAKVGYIGP ATDFN YDHK HLGTDPQTLV EIAEGRHPFF KTLSDAKNPV IIVGAGVFER KDQDAIFAAV ETIAQKANVV RPDWNGLNVL LLHAAQ AAA LDLGLVPQSE KSLESAKFVY LMGADDVNLD KIPDDAFVVY QGHHGDKSVY RANVILPTAA FSEKEGTYQN TEGCTQQ TL PAVPTVGDSR DDWKIIRALS EVAGVRLPYD TIGAVRARIR NVAPNLVNVD EREPATLPSS LRPSFTQKVD TTPFGTVI E NFYMTDAITR ASKIMAQCSA TLLKK

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 1, mitochondrial

Macromolecule #47: NDUS2

• Macromolecule: Name: NDUS2 / type: protein_or_peptide / ID: 47 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 45.031637 KDa

Sequence

String:

MTTRNGQIKN FTLNFGPQHP ANFGSLRLVL EMNGEVVERA EPHIGLLHRG TEKLIEYKTY LQALPYFDRL DYVSTMAQEH AYSLAVERL LNCEVPLRAQ YIRVLFCEIT RILNHLLALT THAMDVGAST PFLWAFEERE KLLEFYERVS GARMHASFIR P GGVAQDLP LGLCRDIDSF TQQFASRIDE LEEMLTGNRI WKQRLVDIGT VTAQQAKDWG FSGVMLRGPG VCWDLRRAAP YD VYDQLDF DVPVGTRGDC YDRYCIRIEE MRQSMRIIWQ CLNKIPSGMI KADDRKLCPP SRCRMKLSME SSIHHFELYT EGF SVPASS TYTAVEAPKG EFGVFLVSNG SNRPYRCKIR APGFAHLQGL DFMSKHHMLA DVVTIIGTQD IVFGEVDR

Macromolecule #48: NDUS3

• Macromolecule: Name: NDUS3 / type: protein_or_peptide / ID: 48 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 22.926971 KDa

Sequence

String:

MDNQFIFKYS WETLPKKWVK KMERSEHGNR FDTKTDYLFQ LLCFLKLHTY TRVQVLIDIC GVDYPSRKRR FEVVYNLLST RYNSRIRVQ TSADEVTRIS SVVSLFPSAG WWEREVWDMF GVSFINHPDL RRILTDYGFE GHPLRKDFPL SGYVEVRYDD P EKRVVSEP IEMTQEFRYF DFASPWEQRS DG

Macromolecule #49: NDUS4

• Macromolecule: Name: NDUS4 / type: protein_or_peptide / ID: 49 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 16.435736 KDa

Sequence

String:

MANTLQRALR ATMARRFSTQ ALVETKPGEI GMVSGIPQEH LRRRVVIYSP ARTASQQGSG KVGRWKINFL STQKWENPLM GWTSTGDPY AHVGDSALTF DSAEAAKAFA EKHGWEYSVK KRHTPLLKVK SYADNFKWKG FPKPAEE

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial

Macromolecule #50: NDUS5

• Macromolecule: Name: NDUS5 / type: protein_or_peptide / ID: 50 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 9.966398 KDa

Sequence

String:

MASGWGITGN KGRCYDFWMD FSECMSRCRE PKDCGLLRED YLECLHHSKE FQRRNRIYKE EQRKIRAAAK KAKDDGVVKE HHY

UniProtKB: Serine/arginine-rich-splicing factor SR34 isoform X2

Macromolecule #51: NDUS6

• Macromolecule: Name: NDUS6 / type: protein_or_peptide / ID: 51 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 11.582296 KDa

Sequence

String:

MASSVLRNLM RFSSPRNTST RSFSLVTSQI SNHTAKWMQD TSKKSPMELI NEVPPIKVEG RIVACEGDTN PALGHPIEFI CLDLPEPAV CKYCGLRYVQ DHHH

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial

Macromolecule #52: NDUS7

• Macromolecule: Name: NDUS7 / type: protein_or_peptide / ID: 52 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 23.710473 KDa

Sequence

String:

MALLSRASSR IYQVHSYQRA LSLHTTLPAL NASTSTHTPT PYAPPPPPSA SSPVGVSKAA EFVISKVDDL MNWARRGSIW PMTFGLACC AVEMMHTGAA RYDLDRFGII FRPSPRQSDC MIVAGTLTNK MAPALRKVYD QMPEPRWVIS MGSCANGGGY Y HYSYSVVR GCDRIVPVDI YVPGCPPTAE ALLYGLLQLQ KKINRRKDFL HWWTK

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial

Macromolecule #53: NDUS8

• Macromolecule: Name: NDUS8 / type: protein_or_peptide / ID: 53 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 25.54501 KDa

Sequence

String:

MAAILARKSL LALRARQLAV SGQGLHSSQS YGLRLSAHSF STKLEDEQRE QLAKEISKDW SSVFERSINT LFLTEMVRGL MLTLKYFFE TKVTINYPFE KGPLSPRFRG EHALRRYPTG EERCIACKLC EAICPAQAIT IEAEEREDGS RRTTRYDIDM T KCIYCGFC QEACPVDAIV EGPNFEFATE THEELLYDKE KLLENGDRWE TEIAENLRSE SLYR

UniProtKB: NADH dehydrogenase [ubiquinone] iron-sulfur protein

Macromolecule #54: NDUP1

• Macromolecule: Name: NDUP1 / type: protein_or_peptide / ID: 54 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 11.245803 KDa

Sequence

String:

MGFIMEFAEN LVLKLMEDPK ERDRRFREHV YRVKDRCEKT KEMWSYPMRP YGFWTFERHN SQLAWDAQIS QVPGRRDPYD DLLQHYSTP PK

UniProtKB: Uncharacterized protein LOC106761134

Macromolecule #55: NDUP4

• Macromolecule: Name: NDUP4 / type: protein_or_peptide / ID: 55 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 7.316445 KDa

Sequence

String:

MVRVASYFAM TLGAFVFWQS MDKVHVWIAL HQDEKQERLE KEAEIRRVRE ELLKQQANQK G

UniProtKB: Uncharacterized protein LOC106767179

Macromolecule #56: NDUB6

• Macromolecule: Name: NDUB6 / type: protein_or_peptide / ID: 56 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 9.708139 KDa

Sequence

String:

MGGGGGDHGH GNGDFRTKVW SMTGGPYCRP KHWKRNTAIA MFGVVLVCIP IAMKSAELEQ RPHHPVRPIP SQLWCKNFGT KDYEQSE

UniProtKB: Uncharacterized protein LOC106758628

Macromolecule #57: NDUV1

• Macromolecule: Name: NDUV1 / type: protein_or_peptide / ID: 57 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 53.90466 KDa

Sequence

String:

MRGILSLRSA LVQHRSEKLG LGLRQFSTQG ASTPTSPQPP PPPASPQPPP PPPPPEKTHF GGLKDEDRIF TNLYGLHDPF LKGAMKRGD WYRTKDLVIK GTDWIVNEMK KSGLRGRGGA GFPSGLKWSF MPKVSDGRPS YLVVNADESE PGTCKDREIM R HDPHKLLE GCLIAGVGMR ATAAYIYIRG EYVNERKNLE KARQEAYAAG LLGKNACGSG YDFDVHIHFG AGAYICGEET AL LESLEGK QGKPRLKPPF PANAGLYGCP TTVTNVETVA VSPTILRRGP EWFAGFGRKN NAGTKLFCVS GHVNKPCTVE EEM SIPLKE LIERHCGGVR GGWDNLLAVI PGGSSVPLLP KSICDDVLMD YDALKAVQSG LGTAAVIVMD KSTDVVDAIA RLSY FYKHE SCGQCTPCRE GTGWLWTIME RMKVGNAKLE EIDMLQELTK QIEGHTICAL GDAAAWPVQG LIRHFRPELE RRIRE NAER ELLQATGS

Macromolecule #58: NDUV2

• Macromolecule: Name: NDUV2 / type: protein_or_peptide / ID: 58 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 27.992949 KDa

Sequence

String:

MLARLAANRF NEIRHIFRQP SRAFSTALNY HLDSPDNNPN LPWEFTEPNK AKVTEILSHY PSNYKQSAVI PLLDLAQQQH GGWLPVSAM NAVANIIEVP PIRVYEVATF YSMFNRAKVG KYHLLVCGTT PCMIRGSRGI EEALLKHLGV KRNEVTPDGL F SVGEMECM GCCVNAPMIT VADYSNGSEG YTYNYYEDVT PEKVVEIVEK LRKGEKPPHG TQNPLRIRSG PEGGNTTLLG EP KPPPCRD LDAC

UniProtKB: NADH dehydrogenase [ubiquinone] flavoprotein

Macromolecule #59: NDUX1

• Macromolecule: Name: NDUX1 / type: protein_or_peptide / ID: 59 / Number of copies: 1 / Enantiomer: LEVO
• Source (natural): Organism: Vigna radiata (mung bean)
• Molecular weight: Theoretical: 10.94954 KDa

Sequence

String:

MNTDITASTK PEYPVVDRNP PFTKVVGNFS TLDYLRFVTI TGVSVTVGYL SGIKPGIRGP SMVTGGLIGV MGGFMYAYQN SAGRLMGFF PNDDEVARHN KK

UniProtKB: Uncharacterized protein LOC106775330

Macromolecule #60: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE

• Macromolecule: Name: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / type: ligand / ID: 60 / Number of copies: 8 / Formula: PC1
• Molecular weight: Theoretical: 790.145 Da

Chemical component information

ChemComp-PC1:
1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / phospholipid*YM

Macromolecule #61: CARDIOLIPIN

• Macromolecule: Name: CARDIOLIPIN / type: ligand / ID: 61 / Number of copies: 8 / Formula: CDL
• Molecular weight: Theoretical: 1.464043 KDa

Chemical component information

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

Macromolecule #62: PROTOPORPHYRIN IX CONTAINING FE

• Macromolecule: Name: PROTOPORPHYRIN IX CONTAINING FE / type: ligand / ID: 62 / Number of copies: 4 / Formula: HEM
• Molecular weight: Theoretical: 616.487 Da

Chemical component information

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

Macromolecule #63: 1,2-Distearoyl-sn-glycerophosphoethanolamine

• Macromolecule: Name: 1,2-Distearoyl-sn-glycerophosphoethanolamine / type: ligand / ID: 63 / Number of copies: 8 / Formula: 3PE
• Molecular weight: Theoretical: 748.065 Da

Chemical component information

ChemComp-3PE:
1,2-Distearoyl-sn-glycerophosphoethanolamine / phospholipid*YM

Macromolecule #64: HEME C

• Macromolecule: Name: HEME C / type: ligand / ID: 64 / Number of copies: 2 / Formula: HEC
• Molecular weight: Theoretical: 618.503 Da

Chemical component information

ChemComp-HEC:
HEME C

Macromolecule #65: UBIQUINONE-10

• Macromolecule: Name: UBIQUINONE-10 / type: ligand / ID: 65 / Number of copies: 1 / Formula: U10
• Molecular weight: Theoretical: 863.343 Da

Chemical component information

ChemComp-U10:
UBIQUINONE-10

Macromolecule #66: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

• Macromolecule: Name: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE; type: ligand / ID: 66 / Number of copies: 1 / Formula: NDP
• Molecular weight: Theoretical: 745.421 Da

Chemical component information

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

Macromolecule #67: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-b...

• Macromolecule: Name: S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate; type: ligand / ID: 67 / Number of copies: 2 / Formula: ZMP
• Molecular weight: Theoretical: 568.704 Da

Chemical component information

ChemComp-ZMP:
S-[2-({N-[(2S)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate

Macromolecule #68: FE (III) ION

• Macromolecule: Name: FE (III) ION / type: ligand / ID: 68 / Number of copies: 1 / Formula: FE
• Molecular weight: Theoretical: 55.845 Da

Macromolecule #69: ZINC ION

• Macromolecule: Name: ZINC ION / type: ligand / ID: 69 / Number of copies: 2 / Formula: ZN
• Molecular weight: Theoretical: 65.409 Da

Macromolecule #70: IRON/SULFUR CLUSTER

• Macromolecule: Name: IRON/SULFUR CLUSTER / type: ligand / ID: 70 / Number of copies: 6 / Formula: SF4
• Molecular weight: Theoretical: 351.64 Da

Chemical component information

ChemComp-FS1:
IRON/SULFUR CLUSTER

Macromolecule #71: FE2/S2 (INORGANIC) CLUSTER

• Macromolecule: Name: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 71 / Number of copies: 2 / Formula: FES
• Molecular weight: Theoretical: 175.82 Da

Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

Macromolecule #72: FLAVIN MONONUCLEOTIDE

• Macromolecule: Name: FLAVIN MONONUCLEOTIDE / type: ligand / ID: 72 / Number of copies: 1 / Formula: FMN
• Molecular weight: Theoretical: 456.344 Da

Chemical component information

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

Experimental details

Structure determination

• Method: cryo EM
• Processing: single particle reconstruction
• Aggregation state: particle

Sample preparation

• Concentration: 1.55 mg/mL
• Buffer: pH: 7.7 ; Details: 0.2% digitonin, 30 mM HEPES pH 7.7, 150 mM potassium acetate, 1 mM EDTA, 0.002% PMSF
• Grid: Model: Quantifoil R1.2/1.3 / Material: COPPER / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 60 sec. / Pretreatment - Atmosphere: AIR
• Vitrification: Cryogen name: ETHANE / Chamber humidity: 90 % / Instrument: LEICA EM GP / Details: 4 ul, 20 seconds pre-blot, blot 4 seconds.
• Details: Digitonin-extracted, amphipol (A8-35)stabilized

Electron microscopy

• Microscope: TFS GLACIOS
• Image recording: Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 2 / Number real images: 25712 / Average exposure time: 3.0 sec. / Average electron dose: 60.0 e/Ų
• Electron beam: Acceleration voltage: 200 kV / Electron source: FIELD EMISSION GUN
• Electron optics: Illumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.5 µm
• Sample stage: Cooling holder cryogen: NITROGEN

Image processing

• Startup model: Type of model: NONE
• Final reconstruction: Number classes used: 1 / Applied symmetry - Point group: C₁ (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.2 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC / Number images used: 123451
• Initial angle assignment: Type: NOT APPLICABLE
• Final angle assignment: Type: NOT APPLICABLE

Atomic model buiding 1

• Refinement: Space: REAL / Protocol: RIGID BODY FIT

Output model

PDB-8e73:
Vigna radiata supercomplex I+III2 (full bridge)