PDB-8e67: ETV6 H396Y variant bound to DNA containing the sequence GGAT

ID or keywords:

Entry

Database: PDB / ID: 8.0E+67

Title

ETV6 H396Y variant bound to DNA containing the sequence GGAT

Components

Complementary 15 bp strand
GGAT-containing 15 bp DNA
Transcription factor ETV6

Keywords

DNA BINDING PROTEIN/DNA / ETS / complex / winged-helix-turn-helix / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex

Function / homology

Function and homology information

sequence-specific DNA binding / DNA-binding transcription factor activity / regulation of transcription by RNA polymerase II / nucleus
Similarity search - Function

Biological species

Mus musculus (house mouse)
synthetic construct (others)

Method

X-RAY DIFFRACTION /

SYNCHROTRON /

MOLECULAR REPLACEMENT / Resolution: 2.3 Å

Authors

Scheu, K. / Chan, A.C. / Murphy, M.E. / McIntosh, L.P.

Funding support

Canada, 1items

Citation

Journal: to be published
Title: The functional role of histidine within the ETV6 ETS domain
Authors: Scheu, K. / Chan, A.C. / Murphy, M.E. / McIntosh, L.P.

History

Deposition	Aug 22, 2022	Deposition site: RCSB / Processing site: RCSB
Revision 1.0	Aug 30, 2023	Provider: repository / Type: Initial release

Structure viewer	Molecule: MolmilJmol/JSmol

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Download

PDBx/mmCIF format	8e67.cif.gz	305.9 KB	Display	PDBx/mmCIF format
PDB format	pdb8e67.ent.gz	243.1 KB	Display	PDB format
PDBx/mmJSON format	8e67.json.gz		Tree view	PDBx/mmJSON format
Others	Other downloads

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Validation report

Arichive directory	https://data.pdbj.org/pub/pdb/validation_reports/e6/8e67 ftp://data.pdbj.org/pub/pdb/validation_reports/e6/8e67	HTTPS FTP

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Related structure data

Related structure data	8e66C 4mhgS S: Starting model for refinement C: citing same article (ref.)
Similar structure data	Similarity search - Function & homologyF&H Search Similarity search - FunctionF&H Search Similarity search - HomologyF&H Search

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Links

PDB pages	PDBj / wwPDB / NCBI
Related items in Molecule of the Month	#67 - Jul 2005 TATA-Binding Protein similarity (1) #168 - Dec 2013 DNA Helicase similarity (1) #255 - Mar 2021 Cisplatin and DNA similarity (1) #91 - Jul 2007 Thymine Dimers similarity (1) #122 - Feb 2010 Enhanceosome similarity (1) #172 - Apr 2014 RecA and Rad51 similarity (1) #181 - Jan 2015 Cascade and CRISPR similarity (1) #253 - Jan 2021 Expressome similarity (1) #112 - Apr 2009 Oct and Sox Transcription Factors similarity (1) #135 - Mar 2011 Integrase similarity (1) #150 - Jun 2012 Sliding Clamps similarity (1) #180 - Dec 2014 TAL Effectors similarity (1) #227 - Nov 2018 Telomerase similarity (1) #48 - Dec 2003 Catabolite Activator Protein similarity (1) #84 - Dec 2006 Transposase similarity (1) #139 - Jul 2011 DNA Methyltransferase similarity (1) #155 - Nov 2012 Vitamin D Receptor similarity (1) #3 - Mar 2000 DNA Polymerase similarity (1) #40 - Apr 2003 RNA Polymerase similarity (1) #73 - Jan 2006 Topoisomerase similarity (1) #87 - Mar 2007 Zinc Fingers similarity (1) #119 - Nov 2009 Designed DNA Crystal similarity (1) #125 - May 2010 Parvoviruses similarity (1) #131 - Nov 2010 Inteins similarity (1) #160 - Apr 2013 Actinomycin similarity (1) #190 - Oct 2015 Two-component Systems similarity (1) #23 - Nov 2001 DNA similarity (1) #240 - Dec 2019 Hypoxia-Inducible Factors similarity (1) #257 - May 2021 Fetal Hemoglobin similarity (1) #258 - Jun 2021 Glucocorticoid Receptor and Dexamethasone similarity (1) #31 - Jul 2002 p53 Tumor Suppressor similarity (1) #33 - Sep 2002 HIV Reverse Transcriptase similarity (1) #39 - Mar 2003 lac Repressor similarity (1) #45 - Sep 2003 Estrogen Receptor similarity (1) #55 - Jul 2004 DNA Ligase similarity (1) #7 - Jul 2000 Nucleosome similarity (1) #8 - Aug 2000 Restriction Enzymes similarity (1) #92 - Aug 2007 Anabolic Steroids similarity (1) #241 - Jan 2020 Twenty Years of Molecules similarity (1)

PDB pages

PDBj /

wwPDB /

NCBI

Related items in Molecule of the Month

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similarity (1)

Deposited unit

B: GGAT-containing 15 bp DNA

C: Complementary 15 bp strand

A: Transcription factor ETV6

D: GGAT-containing 15 bp DNA

E: Complementary 15 bp strand

F: Transcription factor ETV6

G: GGAT-containing 15 bp DNA

H: Complementary 15 bp strand

I: Transcription factor ETV6

J: GGAT-containing 15 bp DNA

K: Complementary 15 bp strand

L: Transcription factor ETV6

86.1 kDa, 12 polymers
Search similar-shape structures of this assembly by Omokage search (details)

1

B: GGAT-containing 15 bp DNA

C: Complementary 15 bp strand

A: Transcription factor ETV6

defined by author&software
Evidence: surface plasmon resonance, Binding between the protein and DNA complex was confirmed through NMR, while affinity was determined through SPR. The dimerization of DNA was confirmed through gel filtration.
21.5 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

2

D: GGAT-containing 15 bp DNA

E: Complementary 15 bp strand

F: Transcription factor ETV6

defined by author&software
21.5 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

3

G: GGAT-containing 15 bp DNA

H: Complementary 15 bp strand

I: Transcription factor ETV6

defined by author&software
21.5 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

4

J: GGAT-containing 15 bp DNA

K: Complementary 15 bp strand

L: Transcription factor ETV6

defined by author&software
21.5 kDa, 3 polymers
Search similar-shape structures of this assembly by Omokage search (details)

Unit cell

Length a, b, c (Å)	63.940, 74.350, 76.620
Angle α, β, γ (deg.)	90.000, 98.020, 90.000
Int Tables number	4
Space group name H-M	P12₁1

#1: DNA chain	GGAT-containing 15 bp DNA Mass: 4683.061 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#2: DNA chain	Complementary 15 bp strand Mass: 4495.922 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others)
#3: Protein	Transcription factor ETV6 Mass: 12337.234 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Etv6 / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: E9Q8J8
#4: Water	ChemComp-HOH / water Mass: 18.015 Da / Num. of mol.: 32 / Source method: isolated from a natural source / Formula: H₂O

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Experiment

Experiment	Method: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

Crystal	Density Matthews: 2.1 Å³/Da / Density % sol: 41.3 %
Crystal grow	Temperature: 298 K / Method: vapor diffusion, hanging drop Details: Protein in 50 mM NaCl, 20 mM HEPES, pH 7.5 at ~300 uM incubated 1:1 with 50 mM Sodium cacodylate, 25 mM Ammonium acetate, 10 mM MgCl2, 20% PEG-8000, pH 6.0 PH range: 6.0-7.5

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Data collection

Diffraction

Mean temperature: 100 K / Serial crystal experiment: N

Diffraction source

Source:

SYNCHROTRON / Site:

CLSI

/ Beamline: 08B1-1 / Wavelength: 1.52127 Å

Detector

Type: DECTRIS PILATUS3 S 6M / Detector: PIXEL / Date: Aug 5, 2021

Radiation

Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray

Radiation wavelength

Wavelength: 1.52127 Å / Relative weight: 1

Reflection

Resolution: 2.3→48.2 Å / Num. obs: 58090 / % possible obs: 99.3 % / Redundancy: 3.287 % / B_iso Wilson estimate: 50.32 Å² / CC_1/2: 0.998 / R_merge(I) obs: 0.062 / R_rim(I) all: 0.074 / Χ²: 0.868 / Net I/σ(I): 10.26

Reflection shell

Diffraction-ID: 1

Resolution (Å)	Redundancy (%)	R_merge(I) obs	Mean I/σ(I) obs	Num. measured obs	Num. possible	Num. unique obs	CC_1/2	R_rim(I) all	% possible all
2.3-2.42	3.208	0.683	1.68	14271	4455	4449	0.748	0.821	99.9
2.42-2.55	3.336	0.474	2.49	13211	3969	3960	0.882	0.563	99.8
2.55-2.69	3.292	0.318	3.66	11187	3412	3398	0.929	0.379	99.6
2.69-2.86	3.452	0.246	4.72	11385	3302	3298	0.967	0.291	99.9
2.86-3.04	3.344	0.157	6.92	9280	2788	2775	0.986	0.187	99.5
3.04-3.25	3.165	0.087	9.92	7760	2477	2452	0.997	0.105	99
3.25-3.5	3.324	0.062	14.51	7443	2263	2239	0.998	0.074	98.9
3.5-3.78	3.076	0.052	17.02	5619	1852	1827	0.997	0.064	98.7
3.78-4.12	3.383	0.047	21.22	5429	1625	1605	0.997	0.056	98.8
4.12-4.51	3.324	0.041	23.41	4385	1336	1319	0.996	0.049	98.7
4.51-5	3.287	0.04	23.81	3635	1120	1106	0.997	0.047	98.8
5-48.2	3.235	0.035	25.53	10165	3178	3142	0.998	0.042	98.9

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Processing

Software

Name	Version	Classification
PHENIX	1.19.2_4158	refinement
XDS		data reduction
XSCALE		data scaling
PDB_EXTRACT	3.27	data extraction
PHENIX		phasing

Refinement

Method to determine structure:

MOLECULAR REPLACEMENT
Starting model: 4MHG

4mhg

Resolution: 2.3→48.2 Å / SU ML: 0.37 / Cross valid method: THROUGHOUT / σ(F): 1.34 / Phase error: 33.77 / Stereochemistry target values: ML

	R_factor	Num. reflection	% reflection
R_free	0.2667	2903	5 %
R_work	0.2113	55187	-
obs	0.214	58090	93.54 %

Solvent computation

Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL

Displacement parameters

B_iso max: 194.81 Å² / B_iso mean: 67.4525 Å² / B_iso min: 23.59 Å²

Refinement step

Cycle: final / Resolution: 2.3→48.2 Å

	Protein	Nucleic acid	Ligand	Solvent	Total
Num. atoms	3233	2390	0	32	5655
B_iso mean	-	-	-	50.48	-
Num. residues	-	-	-	-	493

LS refinement shell

Refine-ID: X-RAY DIFFRACTION / R_factor R_free error: 0 / Total num. of bins used: 21

Resolution (Å)	R_factor R_free	Num. reflection R_free	R_factor R_work	Num. reflection R_work	Num. reflection all	% reflection obs (%)
2.3-2.34	0.3457	132	0.3459	2536	2668	90
2.34-2.38	0.3634	132	0.322	2506	2638	88
2.38-2.42	0.3405	136	0.3257	2573	2709	93
2.42-2.47	0.3555	145	0.3167	2644	2789	93
2.47-2.52	0.3775	131	0.3133	2584	2715	94
2.52-2.57	0.3351	145	0.3089	2684	2829	94
2.57-2.63	0.3764	137	0.301	2575	2712	93
2.63-2.7	0.3459	142	0.2998	2645	2787	94
2.7-2.77	0.3512	140	0.3127	2677	2817	94
2.77-2.85	0.4353	135	0.3149	2648	2783	94
2.85-2.95	0.396	135	0.3106	2649	2784	94
2.95-3.05	0.3487	143	0.2834	2643	2786	93
3.05-3.17	0.3497	129	0.2533	2538	2667	91
3.17-3.32	0.3045	140	0.2256	2590	2730	94
3.32-3.49	0.3453	137	0.2113	2672	2809	94
3.49-3.71	0.224	139	0.197	2654	2793	94
3.71-4	0.2306	141	0.1784	2664	2805	95
4-4.4	0.2111	139	0.1558	2676	2815	95
4.4-5.03	0.2162	142	0.1627	2634	2776	94
5.03-6.34	0.1925	140	0.1606	2683	2823	95
6.34-48.2	0.1789	143	0.1572	2712	2855	97

Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

ID	L11 (°²)	L12 (°²)	L13 (°²)	L22 (°²)	L23 (°²)	L33 (°²)	S11 (Å °)	S12 (Å °)	S13 (Å °)	S21 (Å °)	S22 (Å °)	S23 (Å °)	S31 (Å °)	S32 (Å °)	S33 (Å °)	T11 (Å²)	T12 (Å²)	T13 (Å²)	T22 (Å²)	T23 (Å²)	T33 (Å²)	Origin x (Å)	Origin y (Å)	Origin z (Å)
1	2.1255	0.6714	-2.2296	0.9591	-0.5286	1.3461	0.5732	-0.0847	0.7916	0.1531	0.4127	0.0556	-0.1875	0.3636	0.3927	0.6155	0.1537	-0.0154	0.5816	-0.0199	0.781	-21.0346	-3.1688	-32.2143
2	1.0229	-0.0377	-1.225	0.8418	-0.3714	1.1803	0.3167	0.1896	0.3983	-0.0985	-0.0234	0.1234	-0.9518	-0.2858	-0.0077	0.5158	-0.0203	0.1006	0.45	0.1421	0.5935	-24.3114	-3.1434	-33.6333
3	0.223	-0.145	0.0643	0.0957	-0.0524	0.0338	-0.166	0.8033	0.1395	-0.2604	0.7907	-0.4675	0.7006	-0.2671	0.0004	0.6231	0.167	0.1029	0.8688	0.0888	0.5603	-19.5317	-22.1941	-44.1848
4	0.1241	0.0503	-0.1201	0.0614	-0.0094	0.1339	0.089	0.6085	-0.1906	-0.6178	-0.1577	-0.1103	0.3029	-0.4663	0.0001	0.5156	-0.1347	0.0104	0.5176	0.0246	0.4211	-20.1762	-23.993	-36.1522
5	0.043	0.0942	-0.0554	0.0893	0.0443	0.0228	-0.4629	0.4959	-0.7792	-0.0719	0.1456	-0.839	-0.0631	0.607	-0.0005	0.4834	0.013	-0.017	0.4625	0.0848	0.74	-9.7608	-27.6743	-32.6394
6	0.0398	-0	-0.6541	0.3898	0.9371	2.9381	-0.2779	-0.0476	-0.4902	-0.1432	0.4343	-0.4401	0.5904	1.0329	0.3621	0.5412	-0.1152	-0.0928	0.2841	0.2419	0.4662	-18.3508	-25.7154	-25.3584
7	0.1569	0.059	0.1781	0.7027	0.1155	0.296	1.2649	1.2632	0.1319	0.6878	0.4143	-1.8031	0.6209	0.9621	0.5621	0.0516	-0.3494	0.4584	0.2187	0.6314	0.0505	-11.38	-16.0894	-36.5083
8	4.1545	-0.0797	1.3957	0.1641	0.4305	1.8248	-0.612	-0.187	1.4873	-0.3709	-0.3919	-0.44	-0.6508	-0.6761	-0.3856	0.5041	-0.0531	0.0805	0.4731	0.4015	0.4354	-12.5382	-10.0341	-41.2739
9	0.127	0.1953	0.2231	0.1662	0.3468	0.3207	-0.0502	-0.0908	0.0875	0.0192	-0.0139	0.2902	-0.4142	-0.0425	-0	0.377	0.0281	0.0271	0.2606	0.064	0.4648	-24.4453	-12.2913	-29.9252
10	0.0985	-0.0049	-0.1148	0.0489	0.011	0.063	0.6	-0.2454	0.6911	-0.2876	-0.0889	-0.4601	-0.651	-0.1636	0.0027	0.589	-0.0644	0.0685	0.3993	0.1502	0.5785	-19.808	-16.9975	-18.2262
11	3.2838	-0.502	0.3692	0.0825	-0.1168	0.127	-0.4462	-0.9745	-0.4218	-0.4167	0.0269	0.7878	-0.2685	-0.7191	0.0138	0.4842	-0.1872	-0.0592	0.4351	0.1216	0.6108	-28.8448	-23.3248	-31.2169
12	0.832	1.15	-1.0294	1.637	-0.9345	0.7628	-0.1636	-0.2481	0.0668	-0.0385	0.2916	-0.2822	-0.3499	0.0216	-0	0.5747	0.109	-0.0197	0.5864	-0.1448	0.562	9.6949	-3.6754	-31.5026
13	0.3106	0.3469	-0.5655	1.1158	-0.7015	0.4063	-0.2431	-0.3183	0.3601	0.08	0.1703	-0.1143	-0.8959	0.0313	0.0001	0.6312	0.0453	-0.0611	0.3701	-0.0379	0.5128	6.4377	-3.201	-32.708
14	1.4444	-1.4421	-0.0099	1.5354	-0.7215	1.0483	0.3032	1.1699	-0.3243	-1.2462	0.217	0.7568	0.8802	-0.3301	0.1003	0.4922	0.0347	0.0455	0.5165	0.0268	0.3081	13.1213	-22.4561	-40.0481
15	0.0244	0.0019	-0.0097	0.1325	-0.1646	0.5472	-0.4199	-0.8955	-0.6037	0.2193	0.1097	-0.1986	0.3324	0.7139	-0.0302	0.4272	0.1699	0.1175	0.6047	0.1046	0.6812	23.2511	-25.3294	-33.0659
16	0.9079	-0.5341	-0.012	0.3367	-0.3916	0.4651	0.038	-0.2538	-0.1114	0.0412	-0.1931	-0.3979	0.4338	0.7096	-0.0015	0.3343	0.0088	0.034	0.3257	0.0868	0.389	16.9757	-21.3791	-30.1815
17	0.0437	-0.0098	-0.0083	0.08	0.0187	0.0572	0.1081	0.3469	1.4135	-0.4976	0.1709	-1.1658	-0.7156	0.7233	0.0018	0.5592	-0.1069	0.0407	0.468	0.0505	0.5898	18.1009	-7.7259	-40.7381
18	0.221	-0.3214	-0.0944	0.4916	-0.6374	0.3114	-0.2381	-0.306	0.4589	0.2139	0.2247	-0.0597	-0.029	0.0625	0.0001	0.3467	0.0429	0.0229	0.344	-0.0447	0.3955	8.0403	-14.0354	-27.4032
19	0.4893	-0.0045	-0.0673	0.4059	-0.1464	0.279	-0.0437	-0.462	-0.624	0.1251	-0.0003	0.1482	-0.0997	-0.4978	0.0044	0.3579	-0.0295	0.0383	0.3243	-0.0744	0.5495	2.6402	-24.4268	-33.4369
20	1.7492	-0.8788	-2.4239	1.0031	0.9502	2.0019	-0.186	0.4747	-0.7814	-0.3181	0.1291	0.189	0.5267	0.7574	0.0002	0.8768	0.031	-0.1022	0.7908	0.1824	0.8162	-3.0673	-42.7507	-5.6122
21	1.1709	-0.6656	-0.8484	0.0895	0.3923	0.8114	0.1579	-0.1803	-1.0521	-0.2035	-0.2547	0.1191	0.4006	-0.0384	-0.0041	0.6206	0.0119	-0.0253	0.6258	0.0643	0.7248	-3.1414	-43.9683	-3.6432
22	0.0669	-0.1727	0.2081	0.4752	-0.6065	0.5443	0.0549	-0.2661	0.0764	0.3955	0.6197	-0.4851	-0.0751	0.6674	1.5162	0.5736	-0.1157	-0.2247	1.2286	0.2579	0.6178	6.456	-24.7332	-1.3439
23	0.4786	-0.3585	-0.3115	0.0404	0.2669	0.1562	-0.0089	-0.5093	0.3273	-0.6201	0.1834	-0.3881	-0.2647	0.2297	0.0008	0.5033	-0.0998	0.0627	0.5329	-0.0101	0.4149	-0.114	-20.3171	-8.7017
24	0.2282	-0.2992	0.191	0.089	-0.2549	0.1949	0.7754	0.8303	0.0822	-0.3142	-0.3466	0.2937	-0.7287	-0.3643	0.0095	0.6199	0.0806	0.061	0.3931	0.0324	0.4427	-9.8814	-19.3572	-11.2411
25	0.2364	-0.725	0.1008	0.9554	-0.7265	0.361	0.1455	-0.1989	-0.5498	0.2659	-0.3486	-0.135	0.1044	0.3097	-0.0004	0.3984	0.0068	-0.0028	0.422	0.0295	0.4713	-0.6908	-33.4102	-9.803
26	0.1553	0.2894	-0.2123	0.4097	-0.1202	0.4542	0.167	0.0497	-0.3445	0.4862	0.4557	0.3217	0.2965	-1.116	-0.0044	0.5928	-0.1227	0.0152	0.7101	0.1659	0.594	-14.3104	-28.5785	-8.0362
27	0.0481	0.099	-0.1094	-0.0182	-0.1659	0.1455	-0.2399	-0.4514	0.3599	0.2646	0.313	0.0878	-0.3322	-0.4627	0.0003	0.7204	-0.0086	-0.0446	0.5393	-0.0644	0.4714	-9.5989	-23.1692	1.0256
28	1.6673	0.3993	-1.3326	0.6929	-0.1363	0.9371	0.0936	-0.3452	-0.5077	0.1154	0.0287	-0.3309	0.5837	-0.0177	0.0001	0.6562	0.0335	-0.1272	0.6326	0.1261	0.5926	-36.5289	-45.2197	-8.4704
29	0.5897	-0.7224	-0.3651	0.7999	1.4943	0.5242	0.3463	-0.3524	-0.5716	-0.0732	0.136	-0.052	0.5613	-0.2557	0.0026	0.7379	-0.1238	-0.1845	0.7879	0.268	0.5419	-36.3096	-45.544	-4.8065
30	0.7046	-2.1669	-0.6791	9.5079	-1.5283	3.6043	-0.4321	-1.0599	0.3103	0.2985	0.3638	-1.1656	-0.6904	0.4235	0.0698	0.4631	-0.0346	-0.0758	0.6685	0.0302	0.3271	-30.876	-25.4907	-3.285
31	0.0217	0.0218	-0.0009	0.0523	-0.0664	0.0527	-0.122	-0.4211	0.275	0.454	0.1899	0.4041	0.2948	-0.298	-0.0002	0.7481	-0.2625	-0.029	0.6265	0.0428	0.5884	-28.4002	-19.6448	-14.0694
32	0.2418	0.0472	0.0747	-0.1132	0.0011	0.1381	-0.002	0.3487	0.5129	0.0002	-0.1806	0.2415	0.1775	-0.4158	-0.0037	0.525	-0.0779	0.0394	0.4997	0.0931	0.4855	-40.1654	-21.3689	-11.758
33	0.2322	0.4887	0.0181	0.677	-0.7218	1.0865	0.0457	-0.4431	-0.2231	-0.0546	-0.0584	-0.173	0.6432	0.4606	0.0668	0.4497	-0.0379	-0.0483	0.6169	0.1598	0.4113	-33.1684	-35.2974	-8.5022
34	0.0665	-0.1085	0.0915	0.0824	-0.1463	0.0759	0.0111	-0.3164	-1.0627	-1.3489	0.1905	0.82	0.0199	-0.7438	-0.0001	0.522	-0.08	-0.1065	0.4475	0.0949	0.5821	-46.0468	-29.0525	-16.5814
35	0.2053	-0.0621	0.3329	0.3526	-0.4721	0.5644	0.352	-0.2318	0.4465	0.4141	0.1757	0.5572	0.299	-0.9384	0.1715	0.5956	-0.0383	0.1513	0.6828	-0.0777	0.4792	-41.0533	-25.2905	0.0424

Refinement TLS group

ID	Refine-ID	Refine TLS-ID	Selection details	Auth asym-ID	Auth seq-ID
1	X-RAY DIFFRACTION	1	chain 'B' and (resid 1 through 15 )	B	1 - 15
2	X-RAY DIFFRACTION	2	chain 'C' and (resid 1 through 15 )	C	1 - 15
3	X-RAY DIFFRACTION	3	chain 'A' and (resid 331 through 336 )	A	331 - 336
4	X-RAY DIFFRACTION	4	chain 'A' and (resid 337 through 346 )	A	337 - 346
5	X-RAY DIFFRACTION	5	chain 'A' and (resid 347 through 353 )	A	347 - 353
6	X-RAY DIFFRACTION	6	chain 'A' and (resid 354 through 368 )	A	354 - 368
7	X-RAY DIFFRACTION	7	chain 'A' and (resid 369 through 379 )	A	369 - 379
8	X-RAY DIFFRACTION	8	chain 'A' and (resid 380 through 386 )	A	380 - 386
9	X-RAY DIFFRACTION	9	chain 'A' and (resid 387 through 405 )	A	387 - 405
10	X-RAY DIFFRACTION	10	chain 'A' and (resid 406 through 411 )	A	406 - 411
11	X-RAY DIFFRACTION	11	chain 'A' and (resid 412 through 423 )	A	412 - 423
12	X-RAY DIFFRACTION	12	chain 'D' and (resid 1 through 15 )	D	1 - 15
13	X-RAY DIFFRACTION	13	chain 'E' and (resid 1 through 15 )	E	1 - 15
14	X-RAY DIFFRACTION	14	chain 'F' and (resid 330 through 346 )	F	330 - 346
15	X-RAY DIFFRACTION	15	chain 'F' and (resid 347 through 353 )	F	347 - 353
16	X-RAY DIFFRACTION	16	chain 'F' and (resid 354 through 379 )	F	354 - 379
17	X-RAY DIFFRACTION	17	chain 'F' and (resid 380 through 386 )	F	380 - 386
18	X-RAY DIFFRACTION	18	chain 'F' and (resid 387 through 411 )	F	387 - 411
19	X-RAY DIFFRACTION	19	chain 'F' and (resid 412 through 426 )	F	412 - 426
20	X-RAY DIFFRACTION	20	chain 'G' and (resid 3 through 15 )	G	3 - 15
21	X-RAY DIFFRACTION	21	chain 'H' and (resid 1 through 14 )	H	1 - 14
22	X-RAY DIFFRACTION	22	chain 'I' and (resid 331 through 336 )	I	331 - 336
23	X-RAY DIFFRACTION	23	chain 'I' and (resid 337 through 353 )	I	337 - 353
24	X-RAY DIFFRACTION	24	chain 'I' and (resid 354 through 368 )	I	354 - 368
25	X-RAY DIFFRACTION	25	chain 'I' and (resid 369 through 399 )	I	369 - 399
26	X-RAY DIFFRACTION	26	chain 'I' and (resid 400 through 411 )	I	400 - 411
27	X-RAY DIFFRACTION	27	chain 'I' and (resid 412 through 424 )	I	412 - 424
28	X-RAY DIFFRACTION	28	chain 'J' and (resid 1 through 15 )	J	1 - 15
29	X-RAY DIFFRACTION	29	chain 'K' and (resid 1 through 15 )	K	1 - 15
30	X-RAY DIFFRACTION	30	chain 'L' and (resid 333 through 346 )	L	333 - 346
31	X-RAY DIFFRACTION	31	chain 'L' and (resid 347 through 353 )	L	347 - 353
32	X-RAY DIFFRACTION	32	chain 'L' and (resid 354 through 368 )	L	354 - 368
33	X-RAY DIFFRACTION	33	chain 'L' and (resid 369 through 405 )	L	369 - 405
34	X-RAY DIFFRACTION	34	chain 'L' and (resid 406 through 411 )	L	406 - 411
35	X-RAY DIFFRACTION	35	chain 'L' and (resid 412 through 424 )	L	412 - 424

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