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- PDB-8e2s: Crystal structure of TadAC-1.19 -

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Basic information

Entry
Database: PDB / ID: 8e2s
TitleCrystal structure of TadAC-1.19
ComponentstRNA-specific adenosine deaminase 1.19
KeywordsDNA BINDING PROTEIN / deaminase / TadAC
Function / homology
Function and homology information


tRNA(adenine34) deaminase / tRNA wobble adenosine to inosine editing / tRNA-specific adenosine-34 deaminase activity / zinc ion binding
Similarity search - Function
MafB19-like deaminase / tRNA-specific adenosine deaminase / APOBEC/CMP deaminase, zinc-binding / Cytidine and deoxycytidylate deaminases zinc-binding region signature. / Cytidine and deoxycytidylate deaminase domain / Cytidine and deoxycytidylate deaminases domain profile. / Cytidine deaminase-like
Similarity search - Domain/homology
tRNA-specific adenosine deaminase
Similarity search - Component
Biological speciesEscherichia coli (E. coli)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.95 Å
AuthorsFeliciano, P.R. / Lee, S.J. / Ciaramella, G.
Funding support1items
OrganizationGrant numberCountry
Other private
CitationJournal: Nat.Biotechnol. / Year: 2023
Title: Improved cytosine base editors generated from TadA variants.
Authors: Lam, D.K. / Feliciano, P.R. / Arif, A. / Bohnuud, T. / Fernandez, T.P. / Gehrke, J.M. / Grayson, P. / Lee, K.D. / Ortega, M.A. / Sawyer, C. / Schwaegerle, N.D. / Peraro, L. / Young, L. / ...Authors: Lam, D.K. / Feliciano, P.R. / Arif, A. / Bohnuud, T. / Fernandez, T.P. / Gehrke, J.M. / Grayson, P. / Lee, K.D. / Ortega, M.A. / Sawyer, C. / Schwaegerle, N.D. / Peraro, L. / Young, L. / Lee, S.J. / Ciaramella, G. / Gaudelli, N.M.
History
DepositionAug 15, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 11, 2023Provider: repository / Type: Initial release
Revision 1.1Feb 8, 2023Group: Database references / Category: citation / citation_author
Item: _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed ..._citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation_author.identifier_ORCID / _citation_author.name
Revision 1.2May 31, 2023Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.3Oct 25, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: tRNA-specific adenosine deaminase 1.19
B: tRNA-specific adenosine deaminase 1.19
C: tRNA-specific adenosine deaminase 1.19
D: tRNA-specific adenosine deaminase 1.19
E: tRNA-specific adenosine deaminase 1.19
F: tRNA-specific adenosine deaminase 1.19
G: tRNA-specific adenosine deaminase 1.19
H: tRNA-specific adenosine deaminase 1.19
hetero molecules


Theoretical massNumber of molelcules
Total (without water)149,85616
Polymers149,3328
Non-polymers5238
Water88349
1
A: tRNA-specific adenosine deaminase 1.19
B: tRNA-specific adenosine deaminase 1.19
hetero molecules


Theoretical massNumber of molelcules
Total (without water)37,4644
Polymers37,3332
Non-polymers1312
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2970 Å2
ΔGint-100 kcal/mol
Surface area13630 Å2
MethodPISA
2
C: tRNA-specific adenosine deaminase 1.19
D: tRNA-specific adenosine deaminase 1.19
hetero molecules


Theoretical massNumber of molelcules
Total (without water)37,4644
Polymers37,3332
Non-polymers1312
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2910 Å2
ΔGint-102 kcal/mol
Surface area12810 Å2
MethodPISA
3
E: tRNA-specific adenosine deaminase 1.19
F: tRNA-specific adenosine deaminase 1.19
hetero molecules


Theoretical massNumber of molelcules
Total (without water)37,4644
Polymers37,3332
Non-polymers1312
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2860 Å2
ΔGint-105 kcal/mol
Surface area13020 Å2
MethodPISA
4
G: tRNA-specific adenosine deaminase 1.19
H: tRNA-specific adenosine deaminase 1.19
hetero molecules


Theoretical massNumber of molelcules
Total (without water)37,4644
Polymers37,3332
Non-polymers1312
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area2920 Å2
ΔGint-103 kcal/mol
Surface area12840 Å2
MethodPISA
Unit cell
Length a, b, c (Å)59.495, 63.396, 95.169
Angle α, β, γ (deg.)87.890, 82.820, 77.140
Int Tables number1
Space group name H-MP1
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
11(chain A and ((resid 6 and (name N or name...
21(chain B and ((resid 6 and (name N or name...
31(chain C and ((resid 6 and (name N or name...
41(chain D and ((resid 6 and (name N or name...
51(chain G and ((resid 6 and (name N or name...
61(chain H and ((resid 6 and (name N or name...
71(chain E and (resid 6 through 20 or (resid 22...
81(chain F and ((resid 6 and (name N or name...

NCS domain segments:
Dom-IDComponent-IDEns-IDSelection detailsAuth asym-IDAuth seq-ID
111(chain A and ((resid 6 and (name N or name...A6
121(chain A and ((resid 6 and (name N or name...A3 - 155
131(chain A and ((resid 6 and (name N or name...A3 - 155
141(chain A and ((resid 6 and (name N or name...A3 - 155
151(chain A and ((resid 6 and (name N or name...A3 - 155
211(chain B and ((resid 6 and (name N or name...B6
221(chain B and ((resid 6 and (name N or name...B1 - 151
231(chain B and ((resid 6 and (name N or name...B1 - 151
241(chain B and ((resid 6 and (name N or name...B1 - 151
251(chain B and ((resid 6 and (name N or name...B1 - 151
311(chain C and ((resid 6 and (name N or name...C6
321(chain C and ((resid 6 and (name N or name...C6 - 155
331(chain C and ((resid 6 and (name N or name...C6 - 155
341(chain C and ((resid 6 and (name N or name...C6 - 155
351(chain C and ((resid 6 and (name N or name...C6 - 155
411(chain D and ((resid 6 and (name N or name...D6
421(chain D and ((resid 6 and (name N or name...D6 - 151
431(chain D and ((resid 6 and (name N or name...D6 - 151
441(chain D and ((resid 6 and (name N or name...D6 - 151
451(chain D and ((resid 6 and (name N or name...D6 - 151
511(chain G and ((resid 6 and (name N or name...G6
521(chain G and ((resid 6 and (name N or name...G6 - 151
531(chain G and ((resid 6 and (name N or name...G6 - 151
541(chain G and ((resid 6 and (name N or name...G6 - 151
551(chain G and ((resid 6 and (name N or name...G6 - 151
611(chain H and ((resid 6 and (name N or name...H6
621(chain H and ((resid 6 and (name N or name...H4 - 155
631(chain H and ((resid 6 and (name N or name...H4 - 155
641(chain H and ((resid 6 and (name N or name...H4 - 155
651(chain H and ((resid 6 and (name N or name...H4 - 155
711(chain J and (resid 6 through 20 or (resid 22...E6 - 20
721(chain J and (resid 6 through 20 or (resid 22...E22 - 26
731(chain J and (resid 6 through 20 or (resid 22...E0
741(chain J and (resid 6 through 20 or (resid 22...E0
751(chain J and (resid 6 through 20 or (resid 22...E0
761(chain J and (resid 6 through 20 or (resid 22...E0
811(chain K and ((resid 6 and (name N or name...F6
821(chain K and ((resid 6 and (name N or name...F0
831(chain K and ((resid 6 and (name N or name...F0
841(chain K and ((resid 6 and (name N or name...F0
851(chain K and ((resid 6 and (name N or name...F0

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Components

#1: Protein
tRNA-specific adenosine deaminase 1.19


Mass: 18666.555 Da / Num. of mol.: 8
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli (E. coli) / Gene: tadA / Production host: Escherichia coli (E. coli) / References: UniProt: W8T8U5, tRNA(adenine34) deaminase
#2: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: Zn / Feature type: SUBJECT OF INVESTIGATION
#3: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 49 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.32 Å3/Da / Density % sol: 47.1 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop / Details: PEG 3,350, ammonium citrate tribasic pH 7.0)

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: PETRA III, EMBL c/o DESY / Beamline: P13 (MX1) / Wavelength: 0.97626 Å
DetectorType: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Aug 21, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.97626 Å / Relative weight: 1
ReflectionResolution: 2.95→51.88 Å / Num. obs: 27706 / % possible obs: 98 % / Redundancy: 2 % / CC1/2: 0.918 / Net I/σ(I): 2.9
Reflection shellResolution: 2.95→3.03 Å / Num. unique obs: 2043 / CC1/2: 0.771

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Processing

Software
NameVersionClassification
PHENIX1.19.2_4158refinement
PDB_EXTRACT3.27data extraction
XDSdata reduction
Aimlessdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 8E2P
Resolution: 2.95→51.49 Å / SU ML: 0.32 / Cross valid method: THROUGHOUT / σ(F): 1.96 / Phase error: 28.39 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2607 1363 4.96 %
Rwork0.2186 26130 -
obs0.2207 27493 96.98 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso max: 97.87 Å2 / Biso mean: 32.6496 Å2 / Biso min: 16.55 Å2
Refinement stepCycle: final / Resolution: 2.95→51.49 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8995 0 8 49 9052
Biso mean--27.7 23.57 -
Num. residues----1199
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDNumberRefine-IDRmsType
11A3272X-RAY DIFFRACTION3.092TORSIONAL
12B3272X-RAY DIFFRACTION3.092TORSIONAL
13C3272X-RAY DIFFRACTION3.092TORSIONAL
14D3272X-RAY DIFFRACTION3.092TORSIONAL
15G3272X-RAY DIFFRACTION3.092TORSIONAL
16H3272X-RAY DIFFRACTION3.092TORSIONAL
17E3272X-RAY DIFFRACTION3.092TORSIONAL
18F3272X-RAY DIFFRACTION3.092TORSIONAL
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 10

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.95-3.050.36981430.29982488263193
3.05-3.180.32411500.27062565271595
3.18-3.320.3261290.25552606273596
3.32-3.50.27891430.23452590273396
3.5-3.710.27891390.21322611275097
3.71-40.23481240.19752663278799
4-4.40.21841360.19292627276399
4.4-5.040.23331300.18792699282999
5.04-6.350.24941450.21832645279098
6.35-51.490.18441240.20392636276098
Refinement TLS params.Method: refined / Origin x: -23.276 Å / Origin y: -47.803 Å / Origin z: 12.466 Å
111213212223313233
T0.038 Å20.0551 Å2-0.0137 Å2-0.2938 Å20.1383 Å2--0.3167 Å2
L0.1976 °2-0.0663 °20.0209 °2-0.0828 °2-0.01 °2--0.0714 °2
S0.0054 Å °0.0214 Å °-0.0117 Å °-0.0061 Å °0.029 Å °0.0056 Å °0.04 Å °-0.0385 Å °-0.0373 Å °
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )A3 - 155
2X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )A201
3X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )A301 - 305
4X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )B1 - 151
5X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )B201
6X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )B301 - 313
7X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )C6 - 155
8X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )C201
9X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )C301 - 304
10X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )D6 - 151
11X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )D201
12X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )D301 - 306
13X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )E6 - 160
14X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )E201
15X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )E301 - 302
16X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )F6 - 151
17X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )F201
18X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )F301 - 305
19X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )G6 - 151
20X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )G201
21X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )G301 - 309
22X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )H4 - 155
23X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )H201
24X-RAY DIFFRACTION1( CHAIN A AND ( RESID 3:155 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN B AND ( RESID 1:151 OR RESID 201:201 OR RESID 301:313 ) ) OR ( CHAIN C AND ( RESID 6:155 OR RESID 201:201 OR RESID 301:304 ) ) OR ( CHAIN D AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:306 ) ) OR ( CHAIN E AND ( RESID 6:160 OR RESID 201:201 OR RESID 301:302 ) ) OR ( CHAIN F AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:305 ) ) OR ( CHAIN G AND ( RESID 6:151 OR RESID 201:201 OR RESID 301:309 ) ) OR ( CHAIN H AND ( RESID 4:155 OR RESID 201:201 OR RESID 301:305 ) )H301 - 305

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