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- PDB-8dov: Crystal structure of the Shr Hemoglobin Interacting Domain 2 (HID... -

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Basic information

Entry
Database: PDB / ID: 8dov
TitleCrystal structure of the Shr Hemoglobin Interacting Domain 2 (HID2) in complex with Hemoglobin
Components
  • (Hemoglobin subunit ...) x 2
  • Heme-binding protein Shr
KeywordsTRANSPORT PROTEIN / DUF1533 / hemoglobin / heme / HID / complex / beta-sandwich / globin / Shr
Function / homology
Function and homology information


nitric oxide transport / hemoglobin binding / hemoglobin alpha binding / haptoglobin binding / haptoglobin-hemoglobin complex / renal absorption / organic acid binding / hemoglobin complex / oxygen transport / Scavenging of heme from plasma ...nitric oxide transport / hemoglobin binding / hemoglobin alpha binding / haptoglobin binding / haptoglobin-hemoglobin complex / renal absorption / organic acid binding / hemoglobin complex / oxygen transport / Scavenging of heme from plasma / endocytic vesicle lumen / blood vessel diameter maintenance / hydrogen peroxide catabolic process / oxygen carrier activity / Late endosomal microautophagy / Heme signaling / carbon dioxide transport / response to hydrogen peroxide / Erythrocytes take up oxygen and release carbon dioxide / Erythrocytes take up carbon dioxide and release oxygen / Cytoprotection by HMOX1 / oxygen binding / regulation of blood pressure / platelet aggregation / Chaperone Mediated Autophagy / peroxidase activity / positive regulation of nitric oxide biosynthetic process / tertiary granule lumen / Factors involved in megakaryocyte development and platelet production / ficolin-1-rich granule lumen / blood microparticle / iron ion binding / heme binding / Neutrophil degranulation / extracellular space / extracellular exosome / extracellular region / membrane / metal ion binding / cytosol
Similarity search - Function
Heme-binding protein Shr-like, Hb-interacting domain / Heme-binding protein Shr-like, Hb-interacting domain / NEAT domain / Iron Transport-associated domain / NEAT domain profile. / NEAr Transporter domain / NEAT domain superfamily / Leucine-rich repeat, SDS22-like subfamily / Hemoglobin, pi / Hemoglobin, alpha-type ...Heme-binding protein Shr-like, Hb-interacting domain / Heme-binding protein Shr-like, Hb-interacting domain / NEAT domain / Iron Transport-associated domain / NEAT domain profile. / NEAr Transporter domain / NEAT domain superfamily / Leucine-rich repeat, SDS22-like subfamily / Hemoglobin, pi / Hemoglobin, alpha-type / Hemoglobin, beta-type / Leucine rich repeat / Leucine-rich repeat, typical subtype / Leucine-rich repeats, typical (most populated) subfamily / Globin/Protoglobin / Leucine-rich repeat profile. / Leucine-rich repeat / Globin domain profile. / Globin / Globin / Leucine-rich repeat domain superfamily / Globin-like superfamily / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain.
Similarity search - Domain/homology
PROTOPORPHYRIN IX CONTAINING FE / Heme-binding protein Shr / Hemoglobin subunit beta / Hemoglobin subunit alpha
Similarity search - Component
Biological speciesStreptococcus pyogenes (bacteria)
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.1 Å
AuthorsMacdonald, R. / Mahoney, B.J. / Cascio, D. / Clubb, R.T.
Funding support United States, 4items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R01-AI52217 United States
Department of Energy (DOE, United States)DE-FC02-02ER63421 United States
National Science Foundation (NSF, United States)MCB-1716948 United States
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)GM007185 United States
CitationJournal: Proc.Natl.Acad.Sci.USA / Year: 2023
Title: The Shr receptor from Streptococcus pyogenes uses a cap and release mechanism to acquire heme-iron from human hemoglobin.
Authors: Macdonald, R. / Mahoney, B.J. / Soule, J. / Goring, A.K. / Ford, J. / Loo, J.A. / Cascio, D. / Clubb, R.T.
History
DepositionJul 14, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 25, 2023Provider: repository / Type: Initial release
Revision 1.1Feb 1, 2023Group: Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _citation_author.identifier_ORCID
Revision 1.2Oct 25, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Hemoglobin subunit alpha
B: Hemoglobin subunit beta
C: Hemoglobin subunit alpha
D: Hemoglobin subunit beta
E: Hemoglobin subunit alpha
F: Hemoglobin subunit beta
G: Hemoglobin subunit alpha
H: Hemoglobin subunit beta
I: Heme-binding protein Shr
J: Heme-binding protein Shr
K: Heme-binding protein Shr
hetero molecules


Theoretical massNumber of molelcules
Total (without water)166,41422
Polymers161,20611
Non-polymers5,20811
Water3,009167
1
A: Hemoglobin subunit alpha
B: Hemoglobin subunit beta
C: Hemoglobin subunit alpha
D: Hemoglobin subunit beta
J: Heme-binding protein Shr
K: Heme-binding protein Shr
hetero molecules


Theoretical massNumber of molelcules
Total (without water)89,51913
Polymers86,7776
Non-polymers2,7427
Water1086
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
E: Hemoglobin subunit alpha
F: Hemoglobin subunit beta
G: Hemoglobin subunit alpha
H: Hemoglobin subunit beta
I: Heme-binding protein Shr
hetero molecules


Theoretical massNumber of molelcules
Total (without water)76,8959
Polymers74,4295
Non-polymers2,4664
Water905
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)216.760, 116.450, 70.880
Angle α, β, γ (deg.)90.000, 96.900, 90.000
Int Tables number5
Space group name H-MC121

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Components

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Hemoglobin subunit ... , 2 types, 8 molecules ACEGBDFH

#1: Protein
Hemoglobin subunit alpha / Alpha-globin / Hemoglobin alpha chain


Mass: 15150.353 Da / Num. of mol.: 4 / Fragment: Shr_HID2 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: P69905
#2: Protein
Hemoglobin subunit beta / Beta-globin / Hemoglobin beta chain


Mass: 15890.198 Da / Num. of mol.: 4 / Fragment: Hb_alpha / Source method: isolated from a natural source / Details: blood / Source: (natural) Homo sapiens (human) / References: UniProt: P68871

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Protein , 1 types, 3 molecules IJK

#3: Protein Heme-binding protein Shr


Mass: 12347.937 Da / Num. of mol.: 3 / Fragment: residues 175-285
Source method: isolated from a genetically manipulated source
Details: N-terminal Ser scar after cleavage of tags. Expressed construct comprises amino acid residues 175-285 of the full-length Shr protein.
Source: (gene. exp.) Streptococcus pyogenes (bacteria) / Gene: shr / Plasmid: pHis-SUMO / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: B0LFQ8

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Non-polymers , 3 types, 178 molecules

#4: Chemical
ChemComp-HEM / PROTOPORPHYRIN IX CONTAINING FE / HEME


Mass: 616.487 Da / Num. of mol.: 8 / Source method: obtained synthetically / Formula: C34H32FeN4O4 / Feature type: SUBJECT OF INVESTIGATION
#5: Chemical ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Formula: C3H8O3
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 167 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.67 Å3/Da / Density % sol: 54.01 %
Crystal growTemperature: 295 K / Method: vapor diffusion, hanging drop / pH: 5
Details: 11% PEG-6000, 100mM sodium acetate, 240mM lithium chloride

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9792 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 18, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9792 Å / Relative weight: 1
ReflectionResolution: 2.1→62.43 Å / Num. obs: 98797 / % possible obs: 96.8 % / Redundancy: 3.245 % / Biso Wilson estimate: 59.37 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.043 / Rrim(I) all: 0.052 / Χ2: 0.921 / Net I/σ(I): 13.38 / Num. measured all: 320616 / Scaling rejects: 7
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured obsNum. possibleNum. unique obsCC1/2Rrim(I) all% possible all
2.1-2.153.2870.7651.5923104757570280.5620.91392.8
2.15-2.213.3090.5972.0923771733571830.6790.71397.9
2.21-2.283.2830.472.6922781710369390.7760.56297.7
2.28-2.353.2720.3433.622059690567420.8610.4197.6
2.35-2.423.1930.2644.620892673465430.9170.31897.2
2.42-2.513.0360.1915.8518673651861510.9510.2394.4
2.51-2.63.310.1497.9420230627261120.9710.17897.4
2.6-2.713.3390.1199.7419733602259090.9810.14298.1
2.71-2.833.2950.08912.5618816579357100.9890.10698.6
2.83-2.973.2540.0715.5917776555854620.9920.08398.3
2.97-3.133.1650.05718.1516193523151170.9950.06997.8
3.13-3.323.0610.04720.8414530501047470.9960.05794.8
3.32-3.553.3210.04325.3315375471046290.9960.05198.3
3.55-3.833.2930.03928.0114058434742690.9960.04698.2
3.83-4.23.220.03629.1812699404639440.9970.04397.5
4.2-4.693.0740.03529.6210757365634990.9960.04295.7
4.69-5.423.2960.03430.9510006322230360.9970.04194.2
5.42-6.643.3490.03330.938874271326500.9970.03997.7
6.64-9.383.2050.0331.396411213920000.9980.03693.5
9.38-62.433.4410.02933.553878120111270.9980.03593.8

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Phasing

PhasingMethod: molecular replacement
Phasing MRModel details: Phaser MODE: MR_AUTO
Highest resolutionLowest resolution
Rotation3.1 Å70.94 Å
Translation3.1 Å70.94 Å

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Processing

Software
NameVersionClassification
BUSTER2.10.4 (20-OCT-2021)refinement
XSCALEdata scaling
PHASER2.7.17phasing
PDB_EXTRACT3.24data extraction
XDSBUILT=20170615data reduction
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 6DKQ 1IRD

6dkq

1ird


Resolution: 2.1→62.43 Å / Cor.coef. Fo:Fc: 0.955 / Cor.coef. Fo:Fc free: 0.944 / SU R Cruickshank DPI: 0.215 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.203 / SU Rfree Blow DPI: 0.167 / SU Rfree Cruickshank DPI: 0.174
RfactorNum. reflection% reflectionSelection details
Rfree0.2385 9878 10 %RANDOM
Rwork0.215 ---
obs0.2174 98773 97 %-
Displacement parametersBiso max: 155.47 Å2 / Biso mean: 70.69 Å2 / Biso min: 39.41 Å2
Baniso -1Baniso -2Baniso -3
1-0.4823 Å20 Å2-0.8559 Å2
2--2.4479 Å20 Å2
3----2.9302 Å2
Refine analyzeLuzzati coordinate error obs: 0.3 Å
Refinement stepCycle: final / Resolution: 2.1→62.43 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms11107 0 362 167 11636
Biso mean--59.82 58.15 -
Num. residues----1466
Refine LS restraints
Refine-IDTypeNumberRestraint functionWeightDev ideal
X-RAY DIFFRACTIONt_dihedral_angle_d3945SINUSOIDAL2
X-RAY DIFFRACTIONt_trig_c_planes
X-RAY DIFFRACTIONt_gen_planes2224HARMONIC5
X-RAY DIFFRACTIONt_it11807HARMONIC10
X-RAY DIFFRACTIONt_nbd
X-RAY DIFFRACTIONt_improper_torsion
X-RAY DIFFRACTIONt_pseud_angle
X-RAY DIFFRACTIONt_chiral_improper_torsion1495SEMIHARMONIC5
X-RAY DIFFRACTIONt_sum_occupancies
X-RAY DIFFRACTIONt_utility_distance
X-RAY DIFFRACTIONt_utility_angle
X-RAY DIFFRACTIONt_utility_torsion
X-RAY DIFFRACTIONt_ideal_dist_contact8342SEMIHARMONIC4
X-RAY DIFFRACTIONt_bond_d11807HARMONIC20.008
X-RAY DIFFRACTIONt_angle_deg16092HARMONIC20.86
X-RAY DIFFRACTIONt_omega_torsion2.91
X-RAY DIFFRACTIONt_other_torsion15.49
LS refinement shellResolution: 2.1→2.12 Å / Rfactor Rfree error: 0 / Total num. of bins used: 51
RfactorNum. reflection% reflection
Rfree0.3292 198 10.02 %
Rwork0.3164 1778 -
all0.3178 1976 -
obs--90.52 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
16.2533.5993-2.99936.8274-3.39323.2734-0.1347-0.79250.6486-0.0071-0.0408-0.2198-0.24380.79710.1755-0.13090.0956-0.07090.0161-0.18120.016885.569420.38638.9858
24.2572-0.03580.15642.36590.03452.0072-0.1707-0.3975-0.03110.11060.14350.06830.1640.0740.0272-0.05570.11-0.0118-0.0115-0.0569-0.116179.88442.542238.2723
31.0858-0.5662-0.30442.33860.66951.1725-0.0689-0.09240.1163-0.06740.0819-0.0862-0.06360.136-0.013-0.00640.0358-0.03230.0296-0.0921-0.049979.151913.102132.4896
46.4783-0.51244.09688.3596-7.950915.12740.1654-0.51760.0268-0.1821-0.1874-0.1844-0.26360.77330.022-0.1591-0.23060.0470.146-0.1842-0.159698.37631.02297.7832
54.97450.46540.19436.05040.99584.1091-0.10510.07130.617-0.54290.2164-0.1955-0.77760.3387-0.11130.0741-0.25710.0955-0.135-0.1313-0.196187.409515.91233.7611
63.3805-0.2032-0.10182.86750.63512.4615-0.0618-0.30540.2568-0.0650.2119-0.0041-0.35880.3766-0.15-0.0275-0.19010.07450.1012-0.1592-0.136787.43915.33399.4399
711.247-2.19585.029214.87832.48243.5409-0.173-0.19721.068-0.75170.23742.2960.4747-0.5738-0.0644-0.5797-0.127-0.4424-0.29920.11650.94299.4469-0.0823-4.1509
86.54261.0780.391610.9745-0.89545.3657-0.10980.0883-0.3206-1.42610.04751.01690.7913-0.26340.0623-0.0723-0.1766-0.2378-0.25250.0798-0.073921.5103-14.529-4.8384
95.01191.9661-0.11525.45381.45152.9630.0223-0.22650.1657-0.2158-0.16121.10820.1637-0.45450.1389-0.1918-0.0823-0.12270.01260.07340.059119.6515-3.20330.1508
103.96012.5132-0.12066.80547.28456.9820.08950.06920.033-0.07370.26040.7309-0.4929-0.9462-0.3499-0.20040.08240.06190.0450.30640.112614.1469-18.671929.9196
114.09420.50190.28774.1182-0.5352.86270.0957-0.32360.72160.56290.36340.5557-0.7264-0.6253-0.4591-0.11980.18930.2738-0.15960.27020.206919.4795-0.51530.769
122.3232-0.6596-0.20025.3877-1.88322.24260.24150.20740.3220.01880.25120.5349-0.0083-0.4331-0.4927-0.12520.03910.1137-0.0070.23450.086821.8219-11.157925.3527
1312.866112.0026-1.588232.5063-1.35215.5113-0.2633-0.2528-0.27790.02420.3731.2917-0.4875-0.5691-0.1097-0.35080.1448-0.08780.0239-0.0069-0.066454.934716.124933.4314
144.8267-0.16150.44262.99080.97993.50670.02750.35280.4164-0.51910.04030.1418-0.50280.0548-0.06770.08160.0557-0.0659-0.0708-0.0548-0.090269.600228.378221.9414
1511.85330.7419-1.65243.0955-0.86676.53470.22740.5935-0.4497-0.6760.03560.2333-0.0435-0.336-0.2630.01670.0493-0.185-0.1928-0.0792-0.07459.194515.854915.5254
162.2014-0.17091.15332.0522-0.5285.88310.0208-0.0435-0.1087-0.17620.04770.3982-0.0594-0.2447-0.0684-0.07270.0826-0.0802-0.0532-0.08670.03863.642614.17228.4182
1710.6135-9.88886.982417.9815-6.517711.9074-0.25450.2097-0.2864-0.8390.26850.6665-0.2223-0.2174-0.014-0.0464-0.1989-0.0536-0.082-0.112-0.126870.3626-6.3653-3.6997
183.0299-0.1726-0.35162.04360.30611.9189-0.1464-0.0029-0.3974-0.00560.1596-0.10320.08930.2813-0.0133-0.0064-0.03470.0794-0.0407-0.0945-0.029580.1064-13.2215.4128
195.20051.031.53572.2571-1.32896.1969-0.1470.00040.02040.08830.12640.45380.0758-0.27090.0206-0.0413-0.00910.0672-0.1362-0.06560.047464.1482-6.319614.4587
202.5803-0.0915-1.77552.1812-0.66363.6832-0.09850.2612-0.038-0.23060.14180.2797-0.2231-0.0857-0.04330.0076-0.1123-0.0124-0.0227-0.1067-0.065973.654-1.84714.9902
2118.1854-8.5364-4.410115.51872.33229.3976-0.28020.2451-0.3881-1.18550.0655-0.6665-0.35940.2480.2146-0.0476-0.12760.0369-0.20040.1492-0.159339.26337.9997-7.7415
225.43740.71580.47377.8137-1.3624.4509-0.1566-0.00980.09950.15740.25230.9971-0.4805-0.4387-0.0957-0.03230.0383-0.078-0.14340.1615-0.004324.73414.79678.1189
235.59180.5045-2.92476.80390.78524.4774-0.01250.0537-0.1560.48040.208-0.51550.03190.0553-0.1955-0.0286-0.018-0.1332-0.15650.1138-0.050940.68428.314611.3871
242.49510.13751.85335.4291.53585.73010.01120.26770.0239-0.44570.0126-0.1503-0.02860.0728-0.0238-0.0448-0.0823-0.0242-0.09670.1382-0.019733.65763.5917-0.8314
2514.188112.13183.84925.33964.28896.6606-0.2117-0.58920.55060.74680.1528-0.36620.30050.25140.0589-0.30180.06260.0128-0.0350.0032-0.028745.0438-14.221732.7522
263.38490.6528-0.79372.97760.00982.41740.14350.4037-0.164-0.39240.0525-0.16050.3081-0.118-0.196-0-0.00330.0186-0.02130.1141-0.043434.0438-26.300417.5862
278.2761-0.27441.46013.34450.52474.5010.18390.59330.4782-0.32660.1622-0.2030.02810.1586-0.3461-0.10180.00290.111-0.05590.1385-0.022745.4845-13.847813.8602
283.19540.6583-1.10632.38740.0965.79680.09370.10110.54580.0660.1525-0.055-0.08380.0789-0.2462-0.14390.01550.0556-0.10710.10660.136137.9004-12.024925.3985
2913.7619-1.88658.691317.57546.65716.64890.2401-0.917-0.14392.0304-0.6892-1.8335-0.59240.4530.44910.27510.069-0.7296-0.64140.16820.083346.57232.255521.8751
303.3585-0.13890.831817.159-4.39137.7042-0.4071-0.06860.25691.1850.3582-0.9968-0.4034-0.36370.0488-0.06380.1115-0.1649-0.20630.0654-0.114839.801832.048714.3723
319.7262-2.4789-0.988216.8921-4.550514.9054-0.4509-1.04171.40182.74030.0874-1.9744-1.9210.2260.36350.25480.2358-0.6277-0.6992-0.02-0.303144.445938.737120.8288
3215.25543.81023.953113.19665.16356.55280.0818-0.8789-1.7578-0.7221-0.20890.64060.4704-0.15410.1272-0.07380.1169-0.0210.01780.25320.128197.3114-16.436648.2739
337.5159-0.68273.23491.6598-1.26753.0939-0.027-1.6458-1.0357-0.07160.17850.23190.1183-0.6809-0.1515-0.20260.10970.04470.29370.2187-0.11790.0901-9.51951.7634
343.5504-3.62463.15770.01750.96675.25010.2541-1.3826-1.95670.30520.32190.41950.3496-0.6351-0.576-0.29740.02130.01360.11670.55870.250894.4763-18.461154.3443
3515.2762-0.3017-9.861918.0124-5.75165.8761-0.2388-0.1539-0.78161.173-0.12812.45130.2663-0.21640.3669-0.4866-0.07330.3756-0.6737-0.10440.779555.3454-29.685622.0502
364.137-1.4363-1.178617.99233.50265.2894-0.40040.0291-1.01840.95670.05182.61060.6844-0.03180.3486-0.1593-0.02180.2612-0.3601-0.02370.370864.5828-30.299317.8279
370.8723-4.6181-4.141713.4629-0.07768.9073-0.1288-1.0969-1.80761.3525-0.0412.55341.0726-0.59260.1698-0.22610.03880.6389-0.70710.08550.528959.2974-37.015523.3878
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1{A|1 - 20}A1 - 20
2X-RAY DIFFRACTION2{A|21 - 93}A21 - 93
3X-RAY DIFFRACTION3{A|94 - 142}A94 - 142
4X-RAY DIFFRACTION4{C|1 - 20}C1 - 20
5X-RAY DIFFRACTION5{C|21 - 93}C21 - 93
6X-RAY DIFFRACTION6{C|94 - 142}C94 - 142
7X-RAY DIFFRACTION7{E|1 - 20}E1 - 20
8X-RAY DIFFRACTION8{E|21 - 93}E21 - 93
9X-RAY DIFFRACTION9{E|94 - 142}E94 - 142
10X-RAY DIFFRACTION10{G|1 - 20}G1 - 20
11X-RAY DIFFRACTION11{G|21 - 93}G21 - 93
12X-RAY DIFFRACTION12{G|94 - 142}G94 - 142
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16X-RAY DIFFRACTION16{B|104 - 147}B104 - 147
17X-RAY DIFFRACTION17{D|1 - 23}D1 - 23
18X-RAY DIFFRACTION18{D|24 - 72}D24 - 72
19X-RAY DIFFRACTION19{D|73 - 103}D73 - 103
20X-RAY DIFFRACTION20{D|104 - 147}D104 - 147
21X-RAY DIFFRACTION21{F|1 - 23}F1 - 23
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36X-RAY DIFFRACTION36{K|187 - 258}K187 - 258
37X-RAY DIFFRACTION37{K|259 - 285}K259 - 285

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