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- PDB-8dgp: 14-3-3 epsilon bound to phosphorylated PEAK3 (pS69) peptide -

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Basic information

Entry
Database: PDB / ID: 8dgp
Title14-3-3 epsilon bound to phosphorylated PEAK3 (pS69) peptide
Components
  • 14-3-3 protein epsilon
  • Phosphorylated PEAK3 (pS69) peptide
KeywordsSIGNALING PROTEIN
Function / homology
Function and homology information


regulation of heart rate by hormone / positive regulation of hippo signaling / membrane repolarization during cardiac muscle cell action potential / negative regulation of toll-like receptor signaling pathway / protein localization to endoplasmic reticulum / regulation of membrane repolarization / NADE modulates death signalling / RAB GEFs exchange GTP for GDP on RABs / Signaling by Hippo / regulation of potassium ion transmembrane transport ...regulation of heart rate by hormone / positive regulation of hippo signaling / membrane repolarization during cardiac muscle cell action potential / negative regulation of toll-like receptor signaling pathway / protein localization to endoplasmic reticulum / regulation of membrane repolarization / NADE modulates death signalling / RAB GEFs exchange GTP for GDP on RABs / Signaling by Hippo / regulation of potassium ion transmembrane transport / protein phosphatase inhibitor activity / cytoplasmic pattern recognition receptor signaling pathway / Deregulated CDK5 triggers multiple neurodegenerative pathways in Alzheimer's disease models / negative regulation of calcium ion export across plasma membrane / intracellular potassium ion homeostasis / regulation of heart rate by cardiac conduction / Regulation of HSF1-mediated heat shock response / protein localization to nucleus / phosphoserine residue binding / Activation of BAD and translocation to mitochondria / HSF1 activation / regulation of cytosolic calcium ion concentration / potassium channel regulator activity / protein targeting / Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex / SARS-CoV-2 targets host intracellular signalling and regulatory pathways / calcium channel inhibitor activity / SARS-CoV-1 targets host intracellular signalling and regulatory pathways / RHO GTPases activate PKNs / signaling adaptor activity / Transcriptional and post-translational regulation of MITF-M expression and activity / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / substantia nigra development / Recruitment of mitotic centrosome proteins and complexes / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / protein sequestering activity / regulation of mitotic cell cycle / AURKA Activation by TPX2 / positive regulation of protein export from nucleus / TP53 Regulates Metabolic Genes / calcium channel regulator activity / hippocampus development / Translocation of SLC2A4 (GLUT4) to the plasma membrane / phosphoprotein binding / cerebral cortex development / mitochondrial membrane / histone deacetylase binding / neuron migration / MHC class II protein complex binding / Regulation of PLK1 Activity at G2/M Transition / intracellular protein localization / melanosome / MAPK cascade / cellular response to heat / scaffold protein binding / protein phosphatase binding / transmembrane transporter binding / intracellular signal transduction / cadherin binding / protein heterodimerization activity / protein domain specific binding / focal adhesion / ubiquitin protein ligase binding / enzyme binding / endoplasmic reticulum / signal transduction / RNA binding / extracellular exosome / identical protein binding / nucleus / membrane / cytosol / cytoplasm
Similarity search - Function
14-3-3 domain / Delta-Endotoxin; domain 1 / 14-3-3 proteins signature 2. / 14-3-3 protein, conserved site / 14-3-3 proteins signature 1. / 14-3-3 protein / 14-3-3 homologues / 14-3-3 domain / 14-3-3 domain superfamily / 14-3-3 protein ...14-3-3 domain / Delta-Endotoxin; domain 1 / 14-3-3 proteins signature 2. / 14-3-3 protein, conserved site / 14-3-3 proteins signature 1. / 14-3-3 protein / 14-3-3 homologues / 14-3-3 domain / 14-3-3 domain superfamily / 14-3-3 protein / Up-down Bundle / Mainly Alpha
Similarity search - Domain/homology
14-3-3 protein epsilon
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.7 Å
AuthorsRoy, M.J. / Hardy, J.M. / Lucet, I.S.
Funding support Australia, 1items
OrganizationGrant numberCountry
National Health and Medical Research Council (NHMRC, Australia)APP1144149 Australia
CitationJournal: Nat Commun / Year: 2023
Title: Structural mapping of PEAK pseudokinase interactions identifies 14-3-3 as a molecular switch for PEAK3 signaling.
Authors: Roy, M.J. / Surudoi, M.G. / Kropp, A. / Hou, J. / Dai, W. / Hardy, J.M. / Liang, L.Y. / Cotton, T.R. / Lechtenberg, B.C. / Dite, T.A. / Ma, X. / Daly, R.J. / Patel, O. / Lucet, I.S.
History
DepositionJun 24, 2022Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jun 7, 2023Provider: repository / Type: Initial release
Revision 1.1Jul 12, 2023Group: Data collection / Database references / Category: citation / citation_author / diffrn_source
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _diffrn_source.pdbx_synchrotron_site
Revision 1.2Oct 25, 2023Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / diffrn_source / pdbx_initial_refinement_model
Item: _diffrn_source.pdbx_synchrotron_site
Revision 1.3Oct 23, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: 14-3-3 protein epsilon
B: 14-3-3 protein epsilon
C: 14-3-3 protein epsilon
D: 14-3-3 protein epsilon
E: Phosphorylated PEAK3 (pS69) peptide
F: Phosphorylated PEAK3 (pS69) peptide
G: Phosphorylated PEAK3 (pS69) peptide
H: Phosphorylated PEAK3 (pS69) peptide
hetero molecules


Theoretical massNumber of molelcules
Total (without water)128,20913
Polymers127,7968
Non-polymers4125
Water2,306128
1
A: 14-3-3 protein epsilon
B: 14-3-3 protein epsilon
E: Phosphorylated PEAK3 (pS69) peptide
F: Phosphorylated PEAK3 (pS69) peptide
hetero molecules


Theoretical massNumber of molelcules
Total (without water)64,3109
Polymers63,8984
Non-polymers4125
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4830 Å2
ΔGint-59 kcal/mol
Surface area23500 Å2
MethodPISA
2
C: 14-3-3 protein epsilon
D: 14-3-3 protein epsilon
G: Phosphorylated PEAK3 (pS69) peptide
H: Phosphorylated PEAK3 (pS69) peptide


Theoretical massNumber of molelcules
Total (without water)63,8984
Polymers63,8984
Non-polymers00
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4000 Å2
ΔGint-32 kcal/mol
Surface area22660 Å2
MethodPISA
Unit cell
Length a, b, c (Å)155.532, 155.532, 58.060
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number76
Space group name H-MP41
Space group name HallP4w

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Components

#1: Protein
14-3-3 protein epsilon / 14-3-3E


Mass: 29454.133 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: YWHAE / Production host: Escherichia coli BL21(DE3) (bacteria) / Variant (production host): C41 / References: UniProt: P62258
#2: Protein/peptide
Phosphorylated PEAK3 (pS69) peptide


Mass: 2494.931 Da / Num. of mol.: 4 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)
#3: Chemical ChemComp-EDO / 1,2-ETHANEDIOL / ETHYLENE GLYCOL


Mass: 62.068 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C2H6O2
#4: Chemical ChemComp-SO4 / SULFATE ION


Mass: 96.063 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: SO4
#5: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 128 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.75 Å3/Da / Density % sol: 55.23 %
Crystal growTemperature: 293 K / Method: vapor diffusion, hanging drop / pH: 9.35
Details: 2M ammonium sulfate, 0.1 M sodium bicine pH 9.35, 5% (v/v) 2-methyl-2,4,-pentandiol (MPD)

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Data collection

DiffractionMean temperature: 100 K / Ambient temp details: Liquid nitrogen / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Australian Synchrotron / Beamline: MX2 / Wavelength: 0.9537 Å
DetectorType: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Mar 15, 2020
RadiationMonochromator: M / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9537 Å / Relative weight: 1
ReflectionResolution: 2.69→49.18 Å / Num. obs: 38786 / % possible obs: 99.6 % / Redundancy: 13.9 % / Biso Wilson estimate: 48.69 Å2 / CC1/2: 0.996 / Rmerge(I) obs: 0.256 / Rrim(I) all: 0.266 / Net I/σ(I): 9.71
Reflection shellResolution: 2.69→2.86 Å / Redundancy: 13.6 % / Rmerge(I) obs: 1.786 / Num. unique obs: 82902 / CC1/2: 0.546 / Rrim(I) all: 1.854 / % possible all: 97.7

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Phasing

PhasingMethod: molecular replacement
Phasing MR
Highest resolutionLowest resolution
Rotation3.7 Å49.18 Å
Translation3.7 Å49.18 Å

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
XDS20161205data reduction
Aimless0.5.21data scaling
PHASER2.8.3phasing
PDB_EXTRACT3.27data extraction
Coot0.9.8.1model building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 2BR9

2br9


Resolution: 2.7→49.18 Å / SU ML: 0.3637 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 23.6838
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.233 1985 5.15 %
Rwork0.1874 36526 -
obs0.1898 38511 99.28 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 60.89 Å2
Refinement stepCycle: LAST / Resolution: 2.7→49.18 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms7505 0 23 128 7656
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00617642
X-RAY DIFFRACTIONf_angle_d0.770910362
X-RAY DIFFRACTIONf_chiral_restr0.04371199
X-RAY DIFFRACTIONf_plane_restr0.00691330
X-RAY DIFFRACTIONf_dihedral_angle_d3.71791080
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.7-2.760.41031330.30242379X-RAY DIFFRACTION90.39
2.76-2.840.30291510.25192550X-RAY DIFFRACTION100
2.84-2.920.27131410.21982632X-RAY DIFFRACTION100
2.92-3.020.24521300.21622612X-RAY DIFFRACTION100
3.02-3.130.2531480.21122580X-RAY DIFFRACTION100
3.13-3.250.26991870.20612574X-RAY DIFFRACTION100
3.25-3.40.2861200.23612657X-RAY DIFFRACTION100
3.4-3.580.24831170.18032587X-RAY DIFFRACTION100
3.58-3.80.21121570.16572624X-RAY DIFFRACTION100
3.8-4.090.19341270.15472648X-RAY DIFFRACTION100
4.09-4.510.18311370.15632650X-RAY DIFFRACTION100
4.51-5.160.20851550.16822621X-RAY DIFFRACTION100
5.16-6.50.2821370.21192680X-RAY DIFFRACTION100
6.5-49.180.19771450.16432732X-RAY DIFFRACTION99.55
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
13.3055577018-1.269209561611.745684603977.775069381390.4651286891551.595313100470.168731906479-0.372467129115-0.09638272401650.229465819593-0.1187522621160.311859967943-0.0498007271502-0.392710059856-0.1036065386780.381145977085-0.05571493609050.01043999701060.4214988804930.03504615956130.197158602907-83.979960383448.38012914212.36112511721
21.402104447830.363264344895-1.750471219030.5008450599810.9744600505936.616333483060.04119867398810.093600389463-0.0231866224494-0.274773890729-0.06724077463750.0450283692393-0.407138405350.278811780108-0.03983261254080.3910944251910.00999342033644-0.06221397679690.3946563319170.04205118042710.209085455764-70.834746008556.1068853731-16.1229713004
33.57511816507-0.6813724807371.316752404822.50050717725-0.0460471703572.19583859831-0.0933699413028-0.02697286820410.136121583132-0.1146819141150.01712990177-0.259367675451-0.1472908042780.2531596242580.07763062893370.343693812392-0.0540196425785-0.003957516255030.31973040278-0.03808092955420.196634042871-62.316278797962.28167917930.913322483379
46.609016266192.138947574971.927485304955.304175086351.706545622323.37476666511-0.588755862325-0.975493113936-0.9461390640230.390199293366-0.148986111644-0.1926582924231.013286130050.4389158710210.0887388829830.4551573778160.1495296072590.01968299021520.3311159939230.07279035911030.354831280595-53.516124145949.02824301755.58945123838
57.856808899911.933093135920.7368230966715.88547002934-0.2476832157783.1566409441-0.1803974718950.25792161736-0.0754764287268-0.2028672573880.1466666563570.342363839208-0.218492336056-0.1933362514150.006467249066640.419867155822-0.0308651908029-0.02101227830630.391725433241-0.01347720236950.208426514544-76.196871154244.340677605-32.8199204179
62.838104481150.305966161519-2.372590688152.28270006182-0.05167049423218.0800042598-0.317335353232-0.160819746511-0.6160346772930.19584443994-0.1941404588-0.2290337532940.517191206181-0.02941915859410.0669601033010.443489084706-0.00896135885991-0.08256409992520.3930766630740.0113948674560.299497091493-80.163095518433.085995738-14.8796378186
72.57427368252-0.0133650845007-1.274908464562.274645035751.071257677237.41939991325-0.178706942797-0.120433937261-0.1089463202320.200469638864-0.3095506332060.3367531677480.141652427254-0.5833683621310.09614252097410.365483240047-0.08122643855-0.003175649527960.2925647091490.03249999938840.366648105523-89.694032449835.3791657627-13.522140591
83.986901765911.494260580671.013166337344.31005265148-1.259894022231.4946290646-0.1511750977550.287785147958-0.578751093560.013302402969-0.150928478799-0.0612998616267-0.358943970324-0.085913631471-0.002961146437230.418049924408-0.0132521395382-0.07151131481460.301400539078-0.08334395300070.327751444424-83.157302412627.7500200258-30.4604733778
94.178537440791.47343054166-3.401321365173.35971011898-0.6349680950614.166439435460.1621395438980.36084685318-0.518477544474-0.3142489234320.02159831562210.3204586977630.238770687958-0.3811642401540.2056071714370.5283335702120.0474500104547-0.147739110620.372038829438-0.0996552192780.591462680019-86.849728097219.431608437-28.8743630428
105.84572447163-1.22307680365-0.6685522967163.351583932860.2145769223941.86704203481-0.0479541524482-0.465896034594-1.541180933670.4547246276310.149413031662-0.4527191540810.7849218928540.5725894173480.1854337855650.6024067987940.149840363447-0.1359274994380.419950291557-0.08609868229650.740234766491-74.927189659914.8576649458-26.1636501678
112.235141533491.57847329219-2.608449711346.529450567-1.650049887465.14797629471-0.21196331709-0.708346939406-0.500680248417-0.584237816325-0.278392828183-0.2751571248950.2697155008411.540822519490.3637314903310.5160964865320.137027927781-0.09526630050450.8050026050480.04084370788060.897787440543-64.876595915917.5186784535-25.4939413912
121.099269536981.994903383440.9591112237835.9739608268-1.849645674676.61816175601-0.0882707737911-0.259909189399-0.1535457395341.5490988069-0.2352012833521.575166146570.285939677658-0.0819585803010.4950592647730.67308474671-0.02315784368590.2092106934250.3079725483940.003139486683310.8508831838-56.706456860426.4574292145-1.73172742893
132.42829244422-0.860331742223-0.6464030836296.753946896693.205355322074.3946794068-0.0453511343215-0.127808151918-0.0135485367498-0.07563621158530.02526780493240.381573840715-0.3509549489520.166370322150.1083008052610.406971648839-0.008639198811180.05172168490840.3174185143240.0775953488320.593317963182-47.468790215721.6732346823-16.9644142836
142.3944622035-0.614075745013-0.1807204571777.348694707433.14505170285.20957784538-0.1825820377660.4668859659860.222642148307-0.7052688512170.01001283216280.396768267363-0.2387696249810.0539327761652-0.01805380081130.516191483708-0.06769443666010.1016758546440.3874315817320.09649650810120.575371089072-46.137787147626.4917473793-23.8611124766
152.6924875487-0.26090588217-0.8368663828831.2864152218-0.9267272421546.7271469260.1828269793630.134503092192-0.118207451931-0.153523734802-0.0872216881099-0.446676191015-0.0371338399662-0.246351771997-0.2274998090380.544869404735-0.03576763505940.06071969924710.3462514616910.06198240667010.792623908709-33.310859679533.7733076981-19.9850912338
162.58543089158-0.393462843217-1.46465773493.889036117921.164977621788.100995012040.0100832500384-0.758547064005-0.2833447955670.5176831296290.0160683270273-0.39621066980.1342866003290.845102284733-0.2154902418520.521594030963-0.069116390878-0.0906463747370.5474580053940.1475805328821.01895553787-24.22758921728.0655071875-11.0109657149
176.372907220710.879772556218-0.5143540161885.074049864440.955935514474.80277597904-0.4759189108130.358267543491-0.382651769197-1.363596804460.7379571846650.147437790858-0.1345213495880.261981943754-0.3538489089670.765270978693-0.0912618908659-0.09094107918110.384096969310.05131155848860.864300478944-53.91316966310.573100930748-27.7716388894
185.51963274883-0.853356290159-1.174530614640.170032656850.2896727346830.554159070253-0.1959245730440.449419333118-1.00720854013-0.7061358390530.41694378740.3597834060590.754762034899-0.5738637714650.006346075826790.756732105666-0.351276552771-0.1587793895280.605959268804-0.161571911911.46952108051-59.0694229867-12.4343608485-27.3473935884
191.820587965460.3181696830.1192988112085.36894678892.094917896213.95480491284-0.02911968082-0.281067816843-0.4227158410970.339487061925-0.1775064050141.06635947468-0.196609408232-0.0660329259211-0.2419882433010.6002458042660.005533298024110.1479347477940.3956254753480.2375954508981.07216448653-54.39509359543.91280857258-8.03584631759
201.830615470280.8911441926540.4488414013917.226549950782.876425914923.777878148570.0277191241055-0.1038401831190.2740307833550.4164066126870.03629880210231.45689081149-0.440542336582-0.282613411977-0.1788135200390.6635429338520.07275553710730.3000880462670.3900453070640.3410257631641.31709724211-63.41009875739.47458340618-5.00449419556
212.389216919770.07246020653640.2324140563342.05901826523-1.887934989151.765735492960.3016337402130.153718265461-0.505224570133-0.2088116449550.3604401496440.9857037461460.282886106927-0.349024970491-0.04427485641490.441181657453-0.04340737542730.0411730966830.4426607901180.3063371079431.534836217-60.5887198271-9.60450216697-12.8404040989
221.037569107620.678115785677-0.2001713982631.98218304771-0.3681221716491.533071029730.174548726526-0.00577242227891-0.1710854648910.1173828250980.3787866011150.8345645015860.0798382522119-0.4121345129690.823087125760.579150851816-0.05329195464070.159610813910.3353924181090.2845692008581.56479083451-62.6139128397-13.8010794464-4.45164299466
232.297814890510.177556286739-0.9209536703792.51919691097-1.900318158475.917343020910.175029331284-0.2273792936680.03495986978070.2625193620760.09786728545030.2758567257360.1293397178350.540258325378-0.2523976732430.5045400853930.02793839410210.05667106467010.4320515036320.2177451958861.13803635636-48.1847953354-17.2395657686-3.85593809241
241.82623833729-2.01988097884-2.22756426513.464025585962.483877802357.155621664050.0230405363038-0.05570714190790.167799310408-0.0926728140215-0.0243304933406-0.0265869790579-0.3439319450820.4403460291460.1247086245470.4403611616280.02269145363820.08931536349040.4093611351830.2025701784731.04150647874-40.9347590032-12.0023120163-2.28963767806
258.277416683685.10293879702-4.225983092274.85906714003-1.70067759515.3835097145-0.460827860174-0.901284107276-0.2893909402771.25731254559-0.977499569537-1.78789705399-0.955384895870.9428287329971.078499692960.6013950944870.0471260719046-0.130550762820.5534795618580.04587192526970.727080569111-72.432917657824.9937899869-19.2697437941
269.84772446722-3.43282875305-4.310402244944.26516223975-1.7206698055.27704816276-0.4951023184670.581303242817-1.34409314436-0.8478072825020.188653368137-0.0177719845840.523631683917-0.4733966872270.4920615055790.493447277548-0.03624823662340.01544786560350.405125509244-0.16532663920.315965444595-60.369710998150.0860470597-5.47445288478
275.57117834616-4.33664697929-3.960830813214.173738795745.387991713759.47006896302-0.3281378196790.277158079249-0.09016286216980.3143301585430.983166061382-1.023222210381.1641553558-0.283088774297-0.7520766474190.608437666797-0.0321107151560.1669643373360.4842267902240.07732239272271.24776640148-33.2767625620.5500692272-15.2280642227
280.5446141993291.33151885186-0.8580384977477.244513350731.590683043627.718265142180.867221940909-0.03291644252680.1283512485680.977282061170.424855615686-0.688928991621-0.76379441546-0.13662166833-0.4037517621960.584183956316-0.0841597179476-0.08393434606640.3566159304030.2446822638291.34226374829-46.500227775-4.26080096736-4.70028722634
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid -2 through 38 )AA-2 - 381 - 41
22chain 'A' and (resid 39 through 106 )AA39 - 10642 - 109
33chain 'A' and (resid 107 through 204 )AA107 - 204110 - 207
44chain 'A' and (resid 205 through 233 )AA205 - 233208 - 236
55chain 'B' and (resid 2 through 38 )BD2 - 381 - 37
66chain 'B' and (resid 39 through 73 )BD39 - 7338 - 72
77chain 'B' and (resid 74 through 106 )BD74 - 10673 - 105
88chain 'B' and (resid 107 through 137 )BD107 - 137106 - 136
99chain 'B' and (resid 138 through 162 )BD138 - 162137 - 161
1010chain 'B' and (resid 163 through 204 )BD163 - 204162 - 203
1111chain 'B' and (resid 205 through 234 )BD205 - 234204 - 233
1212chain 'C' and (resid -1 through 17 )CE-1 - 171 - 19
1313chain 'C' and (resid 18 through 73 )CE18 - 7320 - 75
1414chain 'C' and (resid 74 through 138 )CE74 - 13876 - 140
1515chain 'C' and (resid 139 through 187 )CE139 - 187141 - 189
1616chain 'C' and (resid 188 through 234 )CE188 - 234190 - 236
1717chain 'D' and (resid 0 through 33 )DF0 - 331 - 34
1818chain 'D' and (resid 34 through 38 )DF34 - 3835 - 39
1919chain 'D' and (resid 39 through 73 )DF39 - 7340 - 74
2020chain 'D' and (resid 74 through 104 )DF74 - 10475 - 105
2121chain 'D' and (resid 105 through 137 )DF105 - 137106 - 138
2222chain 'D' and (resid 138 through 162 )DF138 - 162139 - 163
2323chain 'D' and (resid 163 through 213 )DF163 - 213164 - 214
2424chain 'D' and (resid 214 through 234 )DF214 - 234215 - 235
2525chain 'E' and (resid 66 through 72 )EG66 - 721 - 7
2626chain 'F' and (resid 65 through 73 )FH65 - 731 - 9
2727chain 'G' and (resid 65 through 72 )GI65 - 721 - 8
2828chain 'H' and (resid 65 through 72 )HJ65 - 721 - 8

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