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- PDB-8cy8: apo form Cryo-EM structure of Campylobacter jejune ketol-acid red... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8cy8 | ||||||
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Title | apo form Cryo-EM structure of Campylobacter jejune ketol-acid reductoisommerase crosslinked by Glutaraldehyde | ||||||
![]() | Ketol-acid reductoisomerase (NADP(+)) | ||||||
![]() | ISOMERASE / ketol-acid reductoisomerase / enzyme stability / dodecamer / branched-chain amino acid | ||||||
Function / homology | ![]() ketol-acid reductoisomerase (NADP+) / ketol-acid reductoisomerase activity / valine biosynthetic process / isoleucine biosynthetic process / isomerase activity / NADP binding / magnesium ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / Resolution: 2.94 Å | ||||||
![]() | Zheng, S. / Guddat, L.W. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Enhancing the Thermal and Kinetic Stability of Ketol-Acid Reductoisomerase, a Central Catalyst of a Cell-Free Enzyme Cascade for the Manufacture of Platform Chemicals Authors: Lv, Y. / Zheng, S. / Goldenzweig, A. / Liu, F. / Gao, Y. / Yang, X. / Kandale, A. / McGeary, R.P. / Williams, S. / Kobe, B. / Schembri, M.A. / Landsberg, M.J. / Wu, B. / Bruck, T.B. / ...Authors: Lv, Y. / Zheng, S. / Goldenzweig, A. / Liu, F. / Gao, Y. / Yang, X. / Kandale, A. / McGeary, R.P. / Williams, S. / Kobe, B. / Schembri, M.A. / Landsberg, M.J. / Wu, B. / Bruck, T.B. / Sieber, V. / Boden, M. / Rao, Z. / Fleishman, S.J. / Schenk, G. / Guddat, L.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 608.5 KB | Display | ![]() |
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PDB format | ![]() | 515.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
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Full document | ![]() | 1.5 MB | Display | |
Data in XML | ![]() | 98.7 KB | Display | |
Data in CIF | ![]() | 148.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 27070MC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 35665.953 Da / Num. of mol.: 12 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-PTD / Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Campylobacter jejuni ketol-acid reductoisomerase / Type: COMPLEX / Entity ID: #1 / Source: RECOMBINANT |
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Molecular weight | Value: 450 kDa/nm / Experimental value: YES |
Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: NO |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 1800 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
Software | Name: PHENIX / Version: 1.19.2_4158: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 2.94 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 74899 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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