+Open data
-Basic information
Entry | Database: PDB / ID: 8cub | |||||||||
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Title | Crystal Structure of ABCG5/G8 in Complex with Cholesterol | |||||||||
Components |
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Keywords | TRANSLOCASE / ABCG5 / ABCG8 / membrane protein / cholesterol transporter | |||||||||
Function / homology | Function and homology information negative regulation of intestinal phytosterol absorption / negative regulation of intestinal cholesterol absorption / Defective ABCG8 causes GBD4 and sitosterolemia / Defective ABCG5 causes sitosterolemia / sterol transport / ABC transporters in lipid homeostasis / intestinal cholesterol absorption / cholesterol transfer activity / triglyceride homeostasis / phospholipid transport ...negative regulation of intestinal phytosterol absorption / negative regulation of intestinal cholesterol absorption / Defective ABCG8 causes GBD4 and sitosterolemia / Defective ABCG5 causes sitosterolemia / sterol transport / ABC transporters in lipid homeostasis / intestinal cholesterol absorption / cholesterol transfer activity / triglyceride homeostasis / phospholipid transport / Translocases; Catalysing the translocation of other compounds; Linked to the hydrolysis of a nucleoside triphosphate / cholesterol efflux / response to muscle activity / bile acid signaling pathway / response to ionizing radiation / ATPase-coupled transmembrane transporter activity / ABC-type transporter activity / NR1H3 & NR1H2 regulate gene expression linked to cholesterol transport and efflux / ATP-binding cassette (ABC) transporter complex / response to nutrient / cholesterol homeostasis / transmembrane transport / receptor complex / response to xenobiotic stimulus / apical plasma membrane / protein heterodimerization activity / ATP hydrolysis activity / ATP binding / metal ion binding / plasma membrane Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 4.05 Å | |||||||||
Authors | Rezaei, F. / Farhat, D. / Lee, J.Y. | |||||||||
Funding support | Canada, 2items
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Citation | Journal: J.Mol.Biol. / Year: 2022 Title: Structural Analysis of Cholesterol Binding and Sterol Selectivity by ABCG5/G8. Authors: Farhat, D. / Rezaei, F. / Ristovski, M. / Yang, Y. / Stancescu, A. / Dzimkova, L. / Samnani, S. / Couture, J.F. / Lee, J.Y. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8cub.cif.gz | 1.1 MB | Display | PDBx/mmCIF format |
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PDB format | pdb8cub.ent.gz | 770.5 KB | Display | PDB format |
PDBx/mmJSON format | 8cub.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cu/8cub ftp://data.pdbj.org/pub/pdb/validation_reports/cu/8cub | HTTPS FTP |
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-Related structure data
Related structure data | 7jr7S S: Starting model for refinement |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments:
NCS ensembles :
NCS oper:
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-Components
#1: Protein | Mass: 74249.148 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ABCG5 / Production host: Komagataella pastoris (fungus) References: UniProt: Q9H222, Translocases; Catalysing the translocation of other compounds; Linked to the hydrolysis of a nucleoside triphosphate #2: Protein | Mass: 77246.156 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: ABCG8 / Production host: Komagataella pastoris (fungus) References: UniProt: Q9H221, Translocases; Catalysing the translocation of other compounds; Linked to the hydrolysis of a nucleoside triphosphate #3: Chemical | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.17 Å3/Da / Density % sol: 70.49 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 1.8-2.0 M ammonium sulfate, 100 mM MES, pH 6.5, 2-5% PEG400, 1 mM TCEP |
-Data collection
Diffraction | Mean temperature: 80 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 0.9794 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Nov 13, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9794 Å / Relative weight: 1 |
Reflection | Resolution: 4.05→30.91 Å / Num. obs: 50917 / % possible obs: 83.96 % / Redundancy: 11.2 % / Biso Wilson estimate: 26.55 Å2 / CC1/2: 0.377 / CC star: 0.74 / Net I/σ(I): 5.9 |
Reflection shell | Resolution: 4.051→4.195 Å / Rmerge(I) obs: 5.094 / Mean I/σ(I) obs: 1.41 / Num. unique obs: 915 / CC1/2: 0.137 / CC star: 0.491 / % possible all: 22.75 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entry 7JR7 Resolution: 4.05→30.91 Å / SU ML: 0.6448 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 30.2978 Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 52.45 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 4.05→30.91 Å
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Refine LS restraints |
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Refine LS restraints NCS |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 47.7562520145 Å / Origin y: 52.6862507304 Å / Origin z: 57.0740364902 Å
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Refinement TLS group | Selection details: all |