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- PDB-8cdb: Proulilysin E229A structure -

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Basic information

Entry
Database: PDB / ID: 8cdb
TitleProulilysin E229A structure
ComponentsUlilysin
KeywordsHYDROLASE / Zymogen / protease / metalloprotease / metzincin
Function / homologyPeptidase M43, pregnancy-associated plasma-A / Pregnancy-associated plasma protein-A / Hydrolases; Acting on peptide bonds (peptidases); Metalloendopeptidases / Metallopeptidase, catalytic domain superfamily / Neutral zinc metallopeptidases, zinc-binding region signature. / metallopeptidase activity / proteolysis / metal ion binding / Ulilysin
Function and homology information
Biological speciesMethanosarcina acetivorans C2A (archaea)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 4.5 Å
AuthorsRodriguez-Banqueri, A. / Eckhard, U. / Gomis-Ruth, F.X.
Funding support Spain, 1items
OrganizationGrant numberCountry
Ministerio de Ciencia e Innovacion (MCIN) Spain
CitationJournal: Dalton Trans / Year: 2023
Title: Structural insights into latency of the metallopeptidase ulilysin (lysargiNase) and its unexpected inhibition by a sulfonyl-fluoride inhibitor of serine peptidases.
Authors: Rodriguez-Banqueri, A. / Moliner-Culubret, M. / Mendes, S.R. / Guevara, T. / Eckhard, U. / Gomis-Ruth, F.X.
History
DepositionJan 30, 2023Deposition site: PDBE / Processing site: PDBE
Revision 1.0Mar 22, 2023Provider: repository / Type: Initial release
Revision 1.1Mar 29, 2023Group: Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation_author.identifier_ORCID
Revision 1.2Nov 6, 2024Group: Data collection / Structure summary
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / pdbx_entry_details / pdbx_modification_feature
Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Ulilysin
B: Ulilysin
C: Ulilysin
D: Ulilysin
E: Ulilysin
F: Ulilysin
G: Ulilysin
H: Ulilysin
I: Ulilysin
J: Ulilysin
K: Ulilysin
L: Ulilysin
M: Ulilysin
N: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)565,07242
Polymers563,59514
Non-polymers1,47728
Water00
1
A: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3623
Polymers40,2571
Non-polymers1052
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
2
B: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3623
Polymers40,2571
Non-polymers1052
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
3
C: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3623
Polymers40,2571
Non-polymers1052
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
4
D: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3623
Polymers40,2571
Non-polymers1052
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
5
E: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3623
Polymers40,2571
Non-polymers1052
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
6
F: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3623
Polymers40,2571
Non-polymers1052
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
7
G: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3623
Polymers40,2571
Non-polymers1052
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
8
H: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3623
Polymers40,2571
Non-polymers1052
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
9
I: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3623
Polymers40,2571
Non-polymers1052
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
10
J: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3623
Polymers40,2571
Non-polymers1052
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
11
K: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3623
Polymers40,2571
Non-polymers1052
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
12
L: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3623
Polymers40,2571
Non-polymers1052
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
13
M: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3623
Polymers40,2571
Non-polymers1052
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
14
N: Ulilysin
hetero molecules


Theoretical massNumber of molelcules
Total (without water)40,3623
Polymers40,2571
Non-polymers1052
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)192.100, 544.400, 185.500
Angle α, β, γ (deg.)90.000, 90.000, 90.000
Int Tables number20
Space group name H-MC2221
Space group name HallC2c2
Symmetry operation#1: x,y,z
#2: x,-y,-z
#3: -x,y,-z+1/2
#4: -x,-y,z+1/2
#5: x+1/2,y+1/2,z
#6: x+1/2,-y+1/2,-z
#7: -x+1/2,y+1/2,-z+1/2
#8: -x+1/2,-y+1/2,z+1/2
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1chain "A"
d_2ens_1chain "B"
d_3ens_1chain "C"
d_4ens_1chain "D"
d_5ens_1chain "E"
d_6ens_1chain "F"
d_7ens_1chain "G"
d_8ens_1chain "H"
d_9ens_1chain "I"
d_10ens_1chain "J"
d_11ens_1chain "K"
d_12ens_1chain "L"
d_13ens_1chain "M"
d_14ens_1chain "N"

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1PROARGA1 - 306
d_12ens_1ZNZNO - P
d_21ens_1PROARGB1 - 306
d_22ens_1ZNZNQ - R
d_31ens_1PROARGC1 - 306
d_32ens_1ZNZNS - T
d_41ens_1PROARGD1 - 306
d_42ens_1ZNZNU - V
d_51ens_1PROARGE1 - 306
d_52ens_1ZNZNW - X
d_61ens_1PROARGF1 - 306
d_62ens_1ZNZNY - Z
d_71ens_1PROARGG1 - 306
d_72ens_1ZNZNAA - BA
d_81ens_1PROARGH1 - 306
d_82ens_1ZNZNCA - DA
d_91ens_1PROARGI1 - 306
d_92ens_1ZNZNEA - FA
d_101ens_1PROARGJ1 - 306
d_102ens_1ZNZNGA - HA
d_111ens_1PROARGK1 - 306
d_112ens_1ZNZNIA - JA
d_121ens_1PROARGL1 - 306
d_122ens_1ZNZNKA - LA
d_131ens_1PROARGM1 - 306
d_132ens_1ZNZNMA - NA
d_141ens_1PROARGN1 - 306
d_142ens_1ZNZNOA - PA

NCS oper:
IDCodeMatrixVector
1given(0.886026266017, -0.0250110855702, -0.46295993512), (-0.0232389495543, -0.99968449755, 0.00953187193302), (-0.463052272591, 0.00231321368094, -0.886327953915)61.6793598341, 506.904222072, 224.61390499
2given(0.901180507059, 0.00261233190455, 0.433436119191), (0.00123554722214, 0.999962293019, -0.00859569451779), (-0.43344223043, 0.00828180313711, 0.901143298603)-30.5741031724, -38.2005880395, 54.0274558653
3given(0.602472473943, -0.0184782735348, -0.797925730597), (-0.0111121508075, -0.999829261734, 0.014763721919), (-0.798062302233, -2.80650175682E-5, -0.602574942199)121.350817766, 466.29022081, 235.149239183
4given(0.629271669856, 0.00697865932041, 0.777154079852), (0.00630000271115, 0.999881026285, -0.0140798878282), (-0.777159877685, 0.0137561473351, 0.629152837495)-34.5416886491, -77.0466566278, 114.919203847
5given(0.204261201425, -0.00976952523064, -0.978867671327), (0.000782626635973, -0.999948249851, 0.010143229983), (-0.978916109321, -0.00283795625533, -0.204242984987)178.687925173, 426.703897929, 218.263957641
6given(0.235201371123, 0.00986509301998, 0.971896596846), (0.0149848162414, 0.999792836213, -0.013774612134), (-0.971831142897, 0.0178034995699, 0.23500481931)-10.9582422235, -116.865405108, 171.197279395
7given(-0.235785380807, -0.00871333512097, -0.971766089133), (0.00192654685716, -0.9999620295, 0.00849870434797), (-0.971803242748, 0.000131717336131, 0.235793214566)225.143529503, 387.808371977, 175.608434561
8given(-0.206883377539, 0.0110018042797, 0.978303750581), (0.00858682021316, 0.999918676805, -0.00942901343714), (-0.978327927955, 0.00644981227336, -0.206961023637)35.7242392311, -155.515410975, 215.473090331
9given(-0.63159616893, -0.00635212109971, -0.775271520147), (0.000629465807806, -0.999970307481, 0.00768035990208), (-0.775297286959, 0.00436287897646, 0.631581413692)248.843739545, 349.151114841, 116.285538401
10given(-0.606381077841, 0.00845286855443, 0.795129258328), (0.0029489360259, 0.999960526549, -0.00838147485438), (-0.795168719337, -0.00273758244097, -0.606382068856)96.5181367091, -193.950454619, 235.029610759
11given(-0.904082159113, 0.00211891152654, -0.427353436615), (-0.00596833474141, -0.999952787338, 0.00766825112769), (-0.427317011776, 0.00948331739875, 0.904052121361)243.805765461, 310.962070149, 51.9788209056
12given(-0.891663375982, 0.00458759546259, 0.452675797786), (-0.00190434111946, 0.999901792279, -0.0138844978849), (-0.452695037987, -0.0132423473933, -0.891567071407)160.335483416, -231.78166354, 227.446937282
13given(-0.999872117776, 0.0158413671435, 0.00219070337237), (-0.0158054181982, -0.999754096883, 0.0155542444761), (0.00243656516897, 0.0155176303818, 0.999876625538)211.058939773, 272.312966215, -4.6535881978

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Components

#1: Protein
Ulilysin


Mass: 40256.789 Da / Num. of mol.: 14
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Methanosarcina acetivorans C2A (archaea)
Gene: MA_3214 / Production host: Escherichia coli BL21(DE3) (bacteria)
References: UniProt: Q8TL28, Hydrolases; Acting on peptide bonds (peptidases); Metalloendopeptidases
#2: Chemical
ChemComp-ZN / ZINC ION


Mass: 65.409 Da / Num. of mol.: 14 / Source method: obtained synthetically / Formula: Zn / Feature type: SUBJECT OF INVESTIGATION
#3: Chemical
ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 14 / Source method: obtained synthetically / Formula: Ca / Feature type: SUBJECT OF INVESTIGATION
Has ligand of interestY
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.3 Å3/Da / Density % sol: 71.41 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop
Details: 100 mM sodium acetate pH 4.5, 1 M diammonium hydrogen phosphate, 10 mM AEBSF

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: ALBA / Beamline: XALOC / Wavelength: 0.979 Å
DetectorType: DECTRIS PILATUS 6M / Detector: PIXEL / Date: May 7, 2021
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.979 Å / Relative weight: 1
ReflectionResolution: 4.5→94.72 Å / Num. obs: 57826 / % possible obs: 99.5 % / Redundancy: 4.4 % / Biso Wilson estimate: 121.7 Å2 / Rrim(I) all: 0.314 / Net I/σ(I): 5.3
Reflection shellResolution: 4.5→4.5 Å / Num. unique obs: 57250 / Rrim(I) all: 0.314

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Processing

Software
NameVersionClassification
PHENIX1.20_4459refinement
XDSdata reduction
XSCALEdata scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT / Resolution: 4.5→94.72 Å / SU ML: 0.582 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 41.9171
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.3021 842 1.47 %
Rwork0.2894 56408 -
obs0.2896 57250 98.57 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 171.2 Å2
Refinement stepCycle: LAST / Resolution: 4.5→94.72 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms33586 0 28 0 33614
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.007534398
X-RAY DIFFRACTIONf_angle_d0.875346760
X-RAY DIFFRACTIONf_chiral_restr0.04945054
X-RAY DIFFRACTIONf_plane_restr0.0086216
X-RAY DIFFRACTIONf_dihedral_angle_d17.563812460
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONcartesian NCS0.0291473123734
ens_1d_3AX-RAY DIFFRACTIONcartesian NCS0.0253077574865
ens_1d_4AX-RAY DIFFRACTIONcartesian NCS0.0285051688296
ens_1d_5AX-RAY DIFFRACTIONcartesian NCS0.0301866073469
ens_1d_6AX-RAY DIFFRACTIONcartesian NCS0.0281477605496
ens_1d_7AX-RAY DIFFRACTIONcartesian NCS0.0317117278139
ens_1d_8AX-RAY DIFFRACTIONcartesian NCS0.0357285636627
ens_1d_9AX-RAY DIFFRACTIONcartesian NCS0.0251040409901
ens_1d_10AX-RAY DIFFRACTIONcartesian NCS0.0288070227276
ens_1d_11AX-RAY DIFFRACTIONcartesian NCS0.0247498386384
ens_1d_12AX-RAY DIFFRACTIONcartesian NCS0.0257449392682
ens_1d_13AX-RAY DIFFRACTIONcartesian NCS0.0265615560377
ens_1d_14AX-RAY DIFFRACTIONcartesian NCS0.0272488553568
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
4.5-4.780.36619376X-RAY DIFFRACTION98.01
4.78-5.150.34959462X-RAY DIFFRACTION98.64
5.15-5.670.38513200.33949133X-RAY DIFFRACTION98.48
5.67-6.490.35242280.31049312X-RAY DIFFRACTION99.29
6.49-8.180.28031440.27539518X-RAY DIFFRACTION99.5
8.18-94.720.22541500.22339607X-RAY DIFFRACTION97.52
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
11.400923339620.7422735324531.056516076550.916098161776-0.1122577827321.57447725157-0.2901156136530.008749807991160.2762662598891.025766478310.147264777996-0.0959397912086-0.1407109142310.3552480859240.001129270300212.987532708260.0368828002631-0.351356061882.0820203748-0.04095616566391.63811793488108.395247.66113.284
20.2626913447050.0104404989657-0.85556328822.414989881950.115755694742.752550013740.0471625041413-0.670506180717-0.1481698303931.93976178867-0.0747144092541-0.291052730360.09483577552130.6799227143330.470677180491.700965281760.1339076604410.5941124192791.457097942320.02612206445010.36179445556699.08257.88374.587
31.733562050010.6641788208531.118274476011.4030384654-0.1136402195922.95321846647-0.180618363766-0.3861472180170.07122979664550.5680454559250.0900115170369-0.293872267126-0.7139825149450.3268142088060.0006625200277062.655436282450.00149457657511-0.1136486535611.944133713290.1406546210651.84347578992116.858208.61111.181
41.6949829972-1.473688077870.4596505486173.64908902866-1.586885543042.708942717240.0199708897123-0.156929090029-0.500062036281-0.5761483350770.1825233728740.8993608134470.0333397710801-0.00607680457671-0.0022518800381.506972149850.0762526721810.03181742554951.794446160320.06538503982160.9569266435691.687219.14180.374
52.48285006922-0.382321489671.016368590142.56925451347-0.396730752460.959336287527-0.152176733418-0.07159646789750.07655509359140.718503640594-0.0464218474397-0.219118813631-0.4869191713180.2072273074840.0009144731872211.55536756635-0.0783033204630.1178180767041.926198197840.08979576351022.00458396228123.436169.671105.359
61.27313876063-1.071831636881.129253157352.109394989720.07711388961982.49037785730.07080655043590.255835116445-0.456581985716-0.3724616622520.2909590645080.509576029642-0.0579710058733-0.290956020177-0.00147547520981.60504427692-0.00891718109801-0.07270133332981.766533315430.1878959798781.5596637251887.519180.29188.314
72.58314753428-0.0867467787315-0.4381366866980.84470351386-0.1324888416692.747295196080.202563063402-0.1107480449080.1155740180790.289941475828-0.3962977240220.0764676705754-0.339092492887-0.3185510508020.0001808066447591.65834176453-0.01320212757210.3075567110931.712995527640.1126694008682.16587453405127.08130.80796.887
81.074320848680.2129731829440.2474767169012.803831002830.3736022148510.3726506771670.1998176079380.2878878046-0.2210419235620.5476805410110.214185050265-0.504240149768-0.07645070595640.1010423088430.3285876547780.2088270752330.05476289229920.5155363286491.984888989330.1302288995420.54278505422187.342141.32997.014
91.10872557701-0.544681122739-0.3963089489983.2980272879-0.9520225515192.32967531158-0.2508768546130.1669638198130.208848517136-0.541996413491-0.17635848456-0.45460156163-0.197548779534-0.2231248187290.0007521298272471.594312136340.02496893709160.1767693965191.949236136860.09035133472961.80920165918126.8591.98787.579
101.514395568010.167579713809-0.3898224317853.157894091051.097091913422.02828039941-0.2695457020320.522433999408-0.136885690008-0.41095635880.1500704584590.7074020673920.200899661925-0.191710969510.0007496946367431.215531209860.09721313474650.06402791991361.83677808840.1290512085311.659634939490.982102.437104.876
111.48566945344-1.53522023328-1.263440960583.429003893420.2348967432821.57577749815-0.07068814861390.3069156379920.438541852631-0.6462208027080.0164247596143-0.2937592051890.164756570353-0.341588096517-4.64745098392E-52.31619035274-0.09128657826590.3730247625991.839193590430.0282345571441.87540329554122.95853.0779.465
122.3777575223-0.0330085866167-1.694386205723.42801551115-0.05906531566092.01231813341-0.137779013250.1983339173120.04591087135610.322492173991-0.008037112979860.7354812873590.491244064427-0.02755314662850.0009507407936951.230828182940.0340745177257-0.005655999436281.670390853770.06003144458231.6023590005497.9263.536110.423
130.37955127773-0.8362121963230.1645771785221.87336640046-0.2248120117482.11749285817-0.106712800888-0.03314884561680.0654110417436-0.908884146890.163661361139-0.297457277832-0.4933020740610.215109177205-0.0003337523981812.26180894169-0.1093319855040.4693661172031.902780558730.05245327542281.78759512116116.10114.07574.097
141.903008293590.583293139537-1.009889623212.64088115773-0.08311489572262.43841572016-0.2831784124520.192198139615-0.27012443191-1.067099722940.00417039603229-0.9780977225660.976023630470.246169468775-0.04562243513710.9050620115240.0271003074784-0.3562868582931.504391087460.09440550717180.945854673806106.84924.763112.724
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1( CHAIN A AND ( RESID 18:323 OR RESID 401:402 ) )A18 - 323
2X-RAY DIFFRACTION1( CHAIN A AND ( RESID 18:323 OR RESID 401:402 ) )A401 - 402
3X-RAY DIFFRACTION2( CHAIN B AND ( RESID 18:323 OR RESID 401:402 ) )B18 - 323
4X-RAY DIFFRACTION2( CHAIN B AND ( RESID 18:323 OR RESID 401:402 ) )B401 - 402
5X-RAY DIFFRACTION3( CHAIN C AND ( RESID 18:323 OR RESID 401:402 ) )C18 - 323
6X-RAY DIFFRACTION3( CHAIN C AND ( RESID 18:323 OR RESID 401:402 ) )C401 - 402
7X-RAY DIFFRACTION4( CHAIN D AND ( RESID 18:323 OR RESID 401:402 ) )D18 - 323
8X-RAY DIFFRACTION4( CHAIN D AND ( RESID 18:323 OR RESID 401:402 ) )D401 - 402
9X-RAY DIFFRACTION5( CHAIN E AND ( RESID 18:323 OR RESID 401:402 ) )E18 - 323
10X-RAY DIFFRACTION5( CHAIN E AND ( RESID 18:323 OR RESID 401:402 ) )E401 - 402
11X-RAY DIFFRACTION6( CHAIN F AND ( RESID 18:323 OR RESID 401:402 ) )F18 - 323
12X-RAY DIFFRACTION6( CHAIN F AND ( RESID 18:323 OR RESID 401:402 ) )F401 - 402
13X-RAY DIFFRACTION7( CHAIN G AND ( RESID 18:323 OR RESID 401:402 ) )G18 - 323
14X-RAY DIFFRACTION7( CHAIN G AND ( RESID 18:323 OR RESID 401:402 ) )G401 - 402
15X-RAY DIFFRACTION8( CHAIN H AND ( RESID 18:323 OR RESID 401:402 ) )H18 - 323
16X-RAY DIFFRACTION8( CHAIN H AND ( RESID 18:323 OR RESID 401:402 ) )H401 - 402
17X-RAY DIFFRACTION9( CHAIN I AND ( RESID 18:323 OR RESID 401:402 ) )I18 - 323
18X-RAY DIFFRACTION9( CHAIN I AND ( RESID 18:323 OR RESID 401:402 ) )I401 - 402
19X-RAY DIFFRACTION10( CHAIN J AND ( RESID 18:323 OR RESID 401:402 ) )J18 - 323
20X-RAY DIFFRACTION10( CHAIN J AND ( RESID 18:323 OR RESID 401:402 ) )J401 - 402
21X-RAY DIFFRACTION11( CHAIN K AND ( RESID 18:323 OR RESID 401:402 ) )K18 - 323
22X-RAY DIFFRACTION11( CHAIN K AND ( RESID 18:323 OR RESID 401:402 ) )K401 - 402
23X-RAY DIFFRACTION12( CHAIN L AND ( RESID 18:323 OR RESID 401:402 ) )L18 - 323
24X-RAY DIFFRACTION12( CHAIN L AND ( RESID 18:323 OR RESID 401:402 ) )L401 - 402
25X-RAY DIFFRACTION13( CHAIN M AND ( RESID 18:323 OR RESID 401:402 ) )M18 - 323
26X-RAY DIFFRACTION13( CHAIN M AND ( RESID 18:323 OR RESID 401:402 ) )M401 - 402
27X-RAY DIFFRACTION14( CHAIN N AND ( RESID 18:323 OR RESID 401:402 ) )N18 - 323
28X-RAY DIFFRACTION14( CHAIN N AND ( RESID 18:323 OR RESID 401:402 ) )N401 - 402

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