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Open data
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Basic information
Entry | Database: PDB / ID: 8ca2 | ||||||
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Title | ENGINEERING THE HYDROPHOBIC POCKET OF CARBONIC ANHYDRASE II | ||||||
![]() | CARBONIC ANHYDRASE II![]() | ||||||
![]() | LYASE(OXO-ACID) | ||||||
Function / homology | ![]() positive regulation of cellular pH reduction / positive regulation of dipeptide transmembrane transport / regulation of monoatomic anion transport / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Alexander, R.S. / Christianson, D.W. | ||||||
![]() | ![]() Title: Engineering the hydrophobic pocket of carbonic anhydrase II. Authors: Alexander, R.S. / Nair, S.K. / Christianson, D.W. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 61 KB | Display | ![]() |
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PDB format | ![]() | 44.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Atom site foot note | 1: RESIDUES 30 AND 202 ARE CIS-PROLINES. |
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Components
#1: Protein | ![]() Mass: 29328.078 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-ZN / |
#3: Chemical | ChemComp-HG / ![]() |
#4: Water | ChemComp-HOH / ![]() |
Compound details | SECONDARY STRUCTURE ELEMENTS WERE DEFINED USING THE PROGRAM *DSSP* (W. KABSCH, C. SANDER, ...SECONDARY STRUCTURE ELEMENTS WERE DEFINED USING THE PROGRAM *DSSP* (W. KABSCH, C. SANDER, BIOPOLYMER |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.14 Å3/Da / Density % sol: 42.61 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow![]() | *PLUS pH: 8 / Method: vapor diffusion, sitting drop | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 6.5 Å / Num. obs: 8367 / Num. measured all: 18465 / Rmerge F obs: 0.085 |
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Processing
Software | Name: PROLSQ / Classification: refinement | |||||||||||||||
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Refinement | Rfactor obs: 0.17 / Highest resolution: 2.4 Å | |||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.4 Å
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Software | *PLUS Name: PROLSQ / Classification: refinement | |||||||||||||||
Refinement | *PLUS Highest resolution: 2.4 Å / Lowest resolution: 6.5 Å / Num. reflection obs: 6990 / Rfactor obs: 0.176 | |||||||||||||||
Solvent computation | *PLUS | |||||||||||||||
Displacement parameters | *PLUS | |||||||||||||||
Refine LS restraints | *PLUS
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