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- PDB-8bw8: Crystal structure of the dCNK-SAM-CRIC-PDZ/dHYP-SAM complex -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 8bw8
TitleCrystal structure of the dCNK-SAM-CRIC-PDZ/dHYP-SAM complex
Components
  • Connector enhancer of KSR protein CNK
  • Protein aveugle
KeywordsSIGNALING PROTEIN / Scaffolding protein / Connector enhancer of KSR (CNK) / Protein Aveugle (AVE) / Hyphen protein (HYP) / COmplex protein / Sterile alpha motif (SAM) domain / conserved region in CNK (CRIC) domain / PSD-95 / Dlg / and Zo-1. (PDZ) domain.
Function / homology
Function and homology information


terminal region determination / Phosphorylation of CI / compound eye cone cell differentiation / Phosphorylation of SMO / torso signaling pathway / tracheal outgrowth, open tracheal system / compound eye photoreceptor cell differentiation / sevenless signaling pathway / Phosphorylation of PER and TIM / MAP2K and MAPK activation ...terminal region determination / Phosphorylation of CI / compound eye cone cell differentiation / Phosphorylation of SMO / torso signaling pathway / tracheal outgrowth, open tracheal system / compound eye photoreceptor cell differentiation / sevenless signaling pathway / Phosphorylation of PER and TIM / MAP2K and MAPK activation / eye photoreceptor cell differentiation / epithelial cell migration, open tracheal system / imaginal disc-derived wing vein specification / MAP-kinase scaffold activity / mitogen-activated protein kinase kinase kinase binding / positive regulation of Ras protein signal transduction / fibroblast growth factor receptor signaling pathway / enzyme regulator activity / cell surface receptor protein tyrosine kinase signaling pathway / visual perception / epidermal growth factor receptor signaling pathway / cytoplasmic side of plasma membrane / receptor tyrosine kinase binding / apical part of cell / insulin receptor signaling pathway / cell cortex / scaffold protein binding / defense response to virus / positive regulation of ERK1 and ERK2 cascade / cytoplasm / cytosol
Similarity search - Function
Aveugle-like, SAM domain / : / CRIC domain / Connector enhancer of kinase suppressor of ras / CRIC domain profile. / SAM domain (Sterile alpha motif) / SAM domain (Sterile alpha motif) / SAM domain profile. / Sterile alpha motif. / Sterile alpha motif domain ...Aveugle-like, SAM domain / : / CRIC domain / Connector enhancer of kinase suppressor of ras / CRIC domain profile. / SAM domain (Sterile alpha motif) / SAM domain (Sterile alpha motif) / SAM domain profile. / Sterile alpha motif. / Sterile alpha motif domain / PH domain / Sterile alpha motif/pointed domain superfamily / PH domain profile. / PDZ domain / Pleckstrin homology domain. / Pleckstrin homology domain / PDZ domain profile. / Domain present in PSD-95, Dlg, and ZO-1/2. / PDZ domain / PDZ superfamily / PH-like domain superfamily
Similarity search - Domain/homology
Connector enhancer of KSR protein CNK / Protein aveugle
Similarity search - Component
Biological speciesDrosophila melanogaster (fruit fly)
MethodX-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.1 Å
AuthorsMaisonneuve, P. / Kurinov, I. / Sicheri, F.
Funding support Canada, 2items
OrganizationGrant numberCountry
Canadian Institutes of Health Research (CIHR)FRN-414829 Canada
Canadian Institutes of Health Research (CIHR)FDN-143277 Canada
CitationJournal: Nat Struct Mol Biol / Year: 2024
Title: The CNK-HYP scaffolding complex promotes RAF activation by enhancing KSR-MEK interaction.
Authors: Pierre Maisonneuve / Malha Sahmi / Fanny Bergeron-Labrecque / Xianjie Iris Ma / Juliette Queguiner / Geneviève Arseneault / Martin Lefrançois / Igor Kurinov / Rémi Fronzes / Frank Sicheri / Marc Therrien /
Abstract: The RAS-MAPK pathway regulates cell proliferation, differentiation and survival, and its dysregulation is associated with cancer development. The pathway minimally comprises the small GTPase RAS and ...The RAS-MAPK pathway regulates cell proliferation, differentiation and survival, and its dysregulation is associated with cancer development. The pathway minimally comprises the small GTPase RAS and the kinases RAF, MEK and ERK. Activation of RAF by RAS is notoriously intricate and remains only partially understood. There are three RAF isoforms in mammals (ARAF, BRAF and CRAF) and two related pseudokinases (KSR1 and KSR2). RAS-mediated activation of RAF depends on an allosteric mechanism driven by the dimerization of its kinase domain. Recent work on human RAFs showed that MEK binding to KSR1 promotes KSR1-BRAF heterodimerization, which leads to the phosphorylation of free MEK molecules by BRAF. Similar findings were made with the single Drosophila RAF homolog. Here we show that the fly scaffold proteins CNK and HYP stabilize the KSR-MEK interaction, which in turn enhances RAF-KSR heterodimerization and RAF activation. The cryogenic electron microscopy structure of the minimal KSR-MEK-CNK-HYP complex reveals a ring-like arrangement of the CNK-HYP complex allowing CNK to simultaneously engage KSR and MEK, thus stabilizing the binary interaction. Together, these results illuminate how CNK contributes to RAF activation by stimulating the allosteric function of KSR and highlight the diversity of mechanisms impacting RAF dimerization as well as the regulatory potential of the KSR-MEK interaction.
History
DepositionDec 6, 2022Deposition site: PDBE / Processing site: PDBE
Revision 1.0Feb 21, 2024Provider: repository / Type: Initial release
Revision 1.1Feb 28, 2024Group: Database references / Category: citation
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN
Revision 1.2Mar 6, 2024Group: Database references / Category: citation / citation_author / Item: _citation.pdbx_database_id_PubMed / _citation.title

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Protein aveugle
B: Connector enhancer of KSR protein CNK
C: Protein aveugle
D: Connector enhancer of KSR protein CNK
E: Protein aveugle
F: Connector enhancer of KSR protein CNK
hetero molecules


Theoretical massNumber of molelcules
Total (without water)155,86315
Polymers155,0346
Non-polymers8299
Water5,098283
1
A: Protein aveugle
B: Connector enhancer of KSR protein CNK
hetero molecules


Theoretical massNumber of molelcules
Total (without water)51,9545
Polymers51,6782
Non-polymers2763
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3700 Å2
ΔGint-9 kcal/mol
Surface area17350 Å2
MethodPISA
2
C: Protein aveugle
D: Connector enhancer of KSR protein CNK
hetero molecules


Theoretical massNumber of molelcules
Total (without water)51,8624
Polymers51,6782
Non-polymers1842
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3600 Å2
ΔGint-10 kcal/mol
Surface area17360 Å2
MethodPISA
3
E: Protein aveugle
F: Connector enhancer of KSR protein CNK
hetero molecules


Theoretical massNumber of molelcules
Total (without water)52,0466
Polymers51,6782
Non-polymers3684
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area4190 Å2
ΔGint-11 kcal/mol
Surface area16700 Å2
MethodPISA
Unit cell
Length a, b, c (Å)131.907, 131.907, 71.465
Angle α, β, γ (deg.)90.000, 90.000, 120.000
Int Tables number144
Space group name H-MP31
Space group name HallP31
Symmetry operation#1: x,y,z
#2: -y,x-y,z+1/3
#3: -x+y,-x,z+2/3
Noncrystallographic symmetry (NCS)NCS domain:
IDEns-IDDetails
d_1ens_1(chain "A" and ((resid 20 through 21 and (name N...
d_2ens_1(chain "C" and (resid 21 through 75 or (resid 76...
d_3ens_1(chain "E" and ((resid 20 through 21 and (name N...
d_1ens_2(chain "B" and (resid 4 through 7 or (resid 8...
d_2ens_2(chain "D" and (resid 4 through 7 or (resid 8...
d_3ens_2(chain "F" and (resid 4 through 39 or (resid 40...

NCS domain segments:
Dom-IDComponent-IDEns-IDBeg label comp-IDEnd label comp-IDLabel asym-IDLabel seq-ID
d_11ens_1LYSTYRA2 - 87
d_21ens_1LYSTYRC1 - 86
d_31ens_1LYSTYRE2 - 87
d_11ens_2ILEALAB2 - 100
d_12ens_2LYSASPB103 - 158
d_13ens_2LEULYSB160 - 201
d_14ens_2PHEASPB205 - 219
d_15ens_2LYSPROB222 - 264
d_21ens_2ILEASPD2 - 156
d_22ens_2LEULYSD158 - 199
d_23ens_2PHEASPD207 - 221
d_24ens_2LYSPROD233 - 275
d_31ens_2ILEALAF1 - 99
d_32ens_2LYSASPF104 - 159
d_33ens_2LEUPROF163 - 262

NCS ensembles :
ID
ens_1
ens_2

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Components

#1: Protein Protein aveugle


Mass: 13143.911 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: ave, CG30476 / Production host: Escherichia coli (E. coli) / References: UniProt: Q8ML92
#2: Protein Connector enhancer of KSR protein CNK / Connector enhancer of ksr / isoform A / isoform B / SD09985p


Mass: 38534.203 Da / Num. of mol.: 3
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Drosophila melanogaster (fruit fly)
Gene: cnk, anon-WO0140519.129, anon-WO0257455.27, CNK, Cnk, Dmel\CG6556, EC2-3, EK2-3, l(2)k16314, sag, CG6556, Dmel_CG6556
Production host: Escherichia coli (E. coli) / References: UniProt: Q7KNQ9
#3: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 9 / Source method: isolated from a natural source / Formula: C3H8O3
#4: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 283 / Source method: isolated from a natural source / Formula: H2O
Has ligand of interestN

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.9 Å3/Da / Density % sol: 57.52 %
Crystal growTemperature: 293.15 K / Method: vapor diffusion, sitting drop / pH: 7
Details: 0.1M Bis-Tris Propane pH 7.0, and 1.0M Ammonium tartrate dibasic

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 0.9792 Å
DetectorType: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Nov 6, 2017
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9792 Å / Relative weight: 1
ReflectionResolution: 2.1→114.23 Å / Num. obs: 81051 / % possible obs: 99.8 % / Redundancy: 5.2 % / Biso Wilson estimate: 49.13 Å2 / CC1/2: 0.999 / Rmerge(I) obs: 0.062 / Rrim(I) all: 0.069 / Net I/σ(I): 14
Reflection shellResolution: 2.1→2.14 Å / Mean I/σ(I) obs: 1 / Num. unique obs: 4475 / CC1/2: 0.397

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Processing

Software
NameVersionClassification
PHENIX1.20.1_4487refinement
XDSdata reduction
HKL2Mapphasing
PHASERphasing
SHELXDEphasing
Aimlessdata scaling
RefinementMethod to determine structure: SAD / Resolution: 2.1→65.95 Å / SU ML: 0.2885 / Cross valid method: FREE R-VALUE / σ(F): 1.96 / Phase error: 25.9199
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2188 4137 5.11 %
Rwork0.1884 76864 -
obs0.1899 81001 99.8 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 63.64 Å2
Refinement stepCycle: LAST / Resolution: 2.1→65.95 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms8397 0 54 283 8734
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00178588
X-RAY DIFFRACTIONf_angle_d0.470411635
X-RAY DIFFRACTIONf_chiral_restr0.03891340
X-RAY DIFFRACTIONf_plane_restr0.00381490
X-RAY DIFFRACTIONf_dihedral_angle_d15.4193153
Refine LS restraints NCS
Ens-IDDom-IDAuth asym-IDRefine-IDTypeRms dev position (Å)
ens_1d_2AX-RAY DIFFRACTIONTorsion NCS0.88465150883
ens_1d_3AX-RAY DIFFRACTIONTorsion NCS0.663617800744
ens_2d_2BX-RAY DIFFRACTIONTorsion NCS0.561764748428
ens_2d_3BX-RAY DIFFRACTIONTorsion NCS0.623205168215
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.1-2.120.35461140.30042547X-RAY DIFFRACTION99.92
2.12-2.150.32071480.28822573X-RAY DIFFRACTION99.93
2.15-2.180.28611920.27462539X-RAY DIFFRACTION100
2.18-2.20.32831450.26572564X-RAY DIFFRACTION99.89
2.2-2.230.29471140.28062559X-RAY DIFFRACTION99.96
2.23-2.260.36471520.30772572X-RAY DIFFRACTION100
2.26-2.290.36691340.28042529X-RAY DIFFRACTION99.81
2.29-2.330.29541480.25922575X-RAY DIFFRACTION99.89
2.33-2.370.29920.2472574X-RAY DIFFRACTION99.85
2.37-2.40.27821360.23752624X-RAY DIFFRACTION99.86
2.4-2.450.24651840.21972524X-RAY DIFFRACTION99.93
2.45-2.490.27061070.23262611X-RAY DIFFRACTION99.82
2.49-2.540.2521260.23322557X-RAY DIFFRACTION100
2.54-2.590.22671800.2142528X-RAY DIFFRACTION99.96
2.59-2.650.2809940.22332603X-RAY DIFFRACTION99.89
2.65-2.710.31111390.24412507X-RAY DIFFRACTION100
2.71-2.780.24761660.24292608X-RAY DIFFRACTION99.86
2.78-2.850.24921440.22532547X-RAY DIFFRACTION99.85
2.85-2.930.24191080.21332575X-RAY DIFFRACTION99.93
2.93-3.030.24771590.21132550X-RAY DIFFRACTION99.93
3.03-3.140.21061340.20292586X-RAY DIFFRACTION99.96
3.14-3.260.21321280.1882565X-RAY DIFFRACTION99.67
3.26-3.410.23611300.18842599X-RAY DIFFRACTION99.89
3.41-3.590.17861100.17752564X-RAY DIFFRACTION99.81
3.59-3.820.20431820.15652501X-RAY DIFFRACTION99.78
3.82-4.110.1871400.14822558X-RAY DIFFRACTION99.78
4.11-4.520.18591360.14542583X-RAY DIFFRACTION99.74
4.53-5.180.16281390.15832530X-RAY DIFFRACTION99.07
5.18-6.520.23071160.18982602X-RAY DIFFRACTION99.93
6.52-65.950.1831400.15492510X-RAY DIFFRACTION98.37
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
14.444171991192.8880117605-1.454377808348.721534239871.052054414347.53494487268-0.1741507517390.485387029624-0.0718534873674-0.1354422514290.3627277646941.326905772970.345411450395-1.01857025316-0.1740069060550.30625992546-0.01416374938080.02362843550370.4746631703760.06811995580130.64653729569737.910267331347.960598929845.5890472007
25.334105757970.569749315107-0.7682767145044.595658459440.3975568831678.83416837533-0.02470235238330.1239458499820.4978037454210.2248289326810.1297744067540.307493248897-0.4991476523040.118590264942-0.1049198365990.2963960686430.0009422247824990.08181721078660.2849860336620.009959985263730.38805058317348.969870347152.662667032648.7603318136
32.989137985294.36019094963-1.268386062054.31978901162-1.828668526391.10873071303-0.2627161628340.3202500376470.0622757852379-0.3169624296780.779161293770.55608145799-0.117675947964-0.33834689759-0.4581878309750.3640678764260.001530354541240.02050819035290.5377480697180.1406477174920.58233201930438.734742856241.365333670357.0013857168
47.84850067616-4.58225689553-1.545750416737.84039157662-0.6442288429867.82658102449-0.03958706067110.295046323217-0.178806989096-0.353958507293-0.0962603970748-0.1171320235690.9742002814240.4573277412460.004945953588380.494448781360.005209355826870.04853038581460.325443422196-0.04572801534760.27429543186562.561186212829.083525402838.5078592585
58.80568818445-0.586504098893-2.498727202726.120014830110.1871275675667.417621613570.04672257844620.07816438026130.042183978304-0.1216224517280.0251491150544-0.3396915164080.1569068954950.370098687287-0.03991623001480.302399694701-0.002095077378110.04499565600150.309120161317-0.001498698876260.26600877189963.803508826540.805383444940.0684098907
66.0608163045-5.168642889382.201005181675.3287146475-3.434302945753.12923701458-0.226514723228-0.448393367575-0.5483115563650.7002708204870.4880569679610.9422915692350.222258705017-0.235648678336-0.2293776663230.420706317441-0.004812471197270.05826018361850.389651936993-0.0259530699460.33901509524356.939387813634.437866715747.4844275743
73.00205379661-3.85369934367-2.255144636475.522312108084.549548993374.930178837020.123302860212-0.364240429550.0875617005139-0.4560061820990.610959601286-0.642367922549-0.3952365028140.0285261195101-0.7943764570740.385314280578-0.00596404559830.02403258654020.4848270336720.03654406649380.43272942936852.12298179428.357815355665.0959394627
84.542280225880.4492891041310.5234163060448.740933259413.113405816663.64689626913-0.198781847906-0.2727653592910.393505989358-0.5304058178790.604577168398-0.698092522089-0.57176449675-0.0616751393233-0.3813058281980.3211620989510.04006959020320.001892729163060.4425337222790.05457650723260.36151860212644.175457847138.126958300163.418459211
96.097375086480.930125375994-0.9167220891782.786256399262.631207838364.85859789399-0.026712580171-0.0978553784121-0.170991411277-0.1600042786690.202071811576-0.1176387477110.0195956596826-0.440427970489-0.1773085556080.257009287556-0.02448735463030.00866224742880.5274923213890.09488601474270.38425256715737.225662758124.048160725762.4688291457
104.37616201204-3.43923563918-1.300341428848.355271848180.1708151367565.19134713874-0.165592921466-0.459829163656-0.2609721377420.6902476985990.3284328272940.59210359587-0.0713945302995-0.540110687463-0.163880671180.3015803021780.0204844408119-0.006993733860450.5741332621650.1170870039920.39200985899644.126280574726.645929632470.0758214142
117.23899015791-3.671061479380.7653932342258.532177550133.407811454966.3963341061-0.3298965104060.314707724844-1.42621582243-0.1299372777070.2725651202861.930897473951.2792553713-0.8250147714410.06968176267210.946576307402-0.2696974542680.04811400119690.655843588904-0.1053200134230.8361910785547.625459356118.118108014240.4571472321
127.592546175066.138443856681.106918682249.07328895621-0.3104388181833.632862438540.480265763022-0.879374210152-0.4086747164370.808904821508-0.6433482296871.668744156181.00405703647-0.8545274233880.123524280230.827758213667-0.349334153080.14910798110.933461314484-0.01513790745510.95664107893643.103575270315.946170750143.2758210122
139.392762980761.494495498770.2358791247364.949531718020.9155023308481.95228254535-0.07769669460.723590323102-0.271116713301-0.8793848874070.2474902860740.7893843665490.794917585745-0.290642540207-0.1590278504820.805439337543-0.169707834373-0.04294174271930.4514802279-0.0779740421640.45655079701451.336409011220.804180284937.2654021675
146.66273833432-2.639234626012.180592368857.326681697590.5658557494417.455072400040.374850831266-0.0315685360118-0.713973337705-0.117809938534-0.14182090144-0.06902283929681.108732196170.0109666850722-0.2617447649210.5759582954120.003938379567720.0307685369380.3505738826930.03532723185910.674592103251-0.96415986869749.051926698151.5220580199
157.223331225220.7462066059635.076195090212.10317957288-0.07277977473735.021821427910.4517885808130.23798118276-0.7950775916760.2988721341610.136673647470.031543600850.282200567147-0.106079010869-0.5696849592010.5828326867170.07381795386640.09173532753310.4365903747450.05416777955730.6124558032894.6877088587652.725519880361.6915279483
168.174293146512.246527092240.6988607050467.9531783505-0.2840386465334.344914406780.03628622254280.1862924841420.15820386502-0.111430956818-0.07485242264950.111444053539-0.257231360008-0.1740237342770.02531491875420.3550436730150.06690604263820.006879138366840.4177300101560.0368291132760.302228455132.5731011673374.264194219545.3382242277
170.648601948037-0.3927473030991.642607773871.350341974240.1421351272313.47384463886-0.1132327624510.0500578389849-0.04818956400860.3672085535560.19090954579-0.07801620218040.06099474100230.126429061318-0.07431667166530.5009782944750.1067568217510.02187670997990.4964293190190.05372135351450.51366027531610.872629139967.663661819564.091932322
187.051767897011.452558546040.4370232542315.824976331960.7197102859993.28565472762-0.0527671044593-0.188786705441-0.7104794804980.729098877169-0.0319915022356-0.4163478899690.6511015762860.3477958590320.1382981193120.6448114172550.187098537625-0.02296724168220.4141443560910.02902764389910.59831636584318.824622063957.269263889471.5468447642
191.79651654754-1.43446000989-0.7910140272232.420357607360.9718205830777.83494827810.2162989409570.1539376359940.210427008274-0.1088654744580.255048039505-0.555025975383-0.2572076692840.410731547449-0.5254650216290.390111283801-0.03206581371310.02685965741330.7222999444190.1421477132440.86454733029830.660294512571.935456635452.4700043793
208.75843888235-3.40720411659-0.2552138902475.434289104550.01301927745582.10072843811-0.05404425812410.148347278053-0.2046047846750.0432421351727-0.123948841223-0.240023805547-0.06154235124550.1457388275770.1758017152430.3644268698140.01172679043090.08740906016530.5918565904220.1044051525090.50902896123525.911670876868.221452008244.9992368552
211.5838894587-0.1012367700362.638234653622.01229067211-0.6786651299424.569786077280.1117320756420.4676804789182.36468389852-0.7548122610760.0453567488011-2.00941950899-1.19254101950.733132500841-0.06424034426410.670661103029-0.1031577655440.0919451354290.4892254630590.01698005561461.12649982335-1.0847683341528.519386286281.9880476555
226.84634407506-0.506459920013-0.9207041316827.65537098809-1.530252601267.877813016260.3204187212040.08916579760661.222117398750.475204861995-0.0468722645424-0.0405448012426-0.813217420675-0.395445410973-0.1864319653480.3941443381260.07897011998930.1023970004790.331615496281-0.007930165078090.573775313455-13.718597771823.685231392180.5702059679
235.234291015611.57617785734-3.24962102932.49585559224-1.598196633173.155984184320.3785621705230.06308502648280.6985566226710.0400626526390.03689115523640.0376671156532-0.410647452389-0.0533501741938-0.4090784647960.4078814444260.04202733826590.04278516200010.3424617832280.04403906353670.547448134107-5.0567489764122.938876368870.795557514
245.853099932160.190391170183-0.8116876610082.32063238676-1.046468809873.3736338589-0.0118777511394-0.326228200687-0.2238060408920.213039308907-0.04364595386560.02729737669380.4359092093870.489077860860.04945942933050.3626512683020.071575951777-0.03134074873570.431946290629-0.008397186381560.2829799127941.331291893862.3906472704786.9481920585
252.06778691361-0.426357776721-3.2575276870.0329276188836-0.3826076732942.178141020460.0669578306243-0.122120768910.403343158508-0.06295058767620.114813119133-0.006136681604080.0604041360548-0.0655873743903-0.1888941661420.361897140787-0.0496643023087-0.05466195372320.3862481679560.02611180114140.4621057163167.1330201476211.391325280768.5345272076
267.29362641033-0.718371493402-1.209274494564.22680330960.1950562135283.790044025610.407809115160.2635291845181.07989461684-0.111422517327-0.000978021626224-0.264715772845-0.610061891080.11314840994-0.3410325974650.456077790208-0.01949228181140.1063083274420.2416326508040.01266788517410.6220302934959.3856430395225.221054626263.2998782987
277.72340799877-2.163447829580.3559047746074.830651199960.6909816940982.854397183680.4766773019221.056996639411.65064716259-0.388313998613-0.1017334139950.081807706986-0.725932876289-0.656435432901-0.3425403802470.5407011903620.09314264418260.1210706397670.5839512370760.2931545809980.7040664179464.9616157218725.601374786254.9250512597
281.419886359220.694430831276-1.355773214881.05007735261-0.2922396438190.743277093490.0853218394884-0.9306488344160.559311501110.331843735769-0.0969492693307-0.367310947749-0.4125671419040.7878161006620.1149332350740.426799890233-0.00771875547145-0.0813811684720.89602454954-0.09162731410690.51745846421324.790523239815.112084536575.3185311282
296.284918198443.73385028247-1.256356815598.536045531141.526842058986.972199715640.369889796512-0.8283364100441.116000650440.385417186781-0.2966565708250.216943493422-0.8770812579340.831854023094-0.02275905282110.531410274118-0.125882683289-0.07938744618820.859783424672-0.1219354498590.66553042272116.157005939516.06035289587.3222088472
303.303489544182.303372780972.308632766912.165749933363.870063603142.211238590260.393768914113-1.14532570009-0.05664095693270.2308265385890.515267615871-1.73601376465-0.8909253511092.22545909533-0.5299013148920.492085752725-0.128663534765-0.2110475283121.54013990986-0.1922232814480.6409215968924.7949994512.219436918687.2084310594
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'A' and (resid 19 through 38 )AA19 - 381 - 20
22chain 'A' and (resid 39 through 73 )AA39 - 7321 - 55
33chain 'A' and (resid 74 through 105 )AA74 - 10556 - 87
44chain 'B' and (resid 3 through 22 )BB3 - 221 - 20
55chain 'B' and (resid 23 through 57 )BB23 - 5721 - 55
66chain 'B' and (resid 58 through 76 )BB58 - 7656 - 74
77chain 'B' and (resid 77 through 101 )BB77 - 10175 - 99
88chain 'B' and (resid 102 through 133 )BB102 - 133100 - 126
99chain 'B' and (resid 134 through 163 )BB134 - 163127 - 156
1010chain 'B' and (resid 164 through 202 )BB164 - 202157 - 193
1111chain 'B' and (resid 203 through 227 )BB203 - 227194 - 214
1212chain 'B' and (resid 228 through 249 )BB228 - 249215 - 227
1313chain 'B' and (resid 250 through 286 )BB250 - 286228 - 264
1414chain 'C' and (resid 21 through 59 )CC21 - 591 - 39
1515chain 'C' and (resid 60 through 106 )CC60 - 10640 - 86
1616chain 'D' and (resid 3 through 57 )DD3 - 571 - 55
1717chain 'D' and (resid 58 through 112 )DD58 - 11256 - 103
1818chain 'D' and (resid 113 through 191 )DD113 - 191104 - 180
1919chain 'D' and (resid 192 through 218 )DD192 - 218181 - 207
2020chain 'D' and (resid 219 through 286 )DD219 - 286208 - 275
2121chain 'E' and (resid 19 through 26 )EE19 - 261 - 8
2222chain 'E' and (resid 27 through 58 )EE27 - 589 - 40
2323chain 'E' and (resid 59 through 105 )EE59 - 10541 - 87
2424chain 'F' and (resid 4 through 57 )FF4 - 571 - 54
2525chain 'F' and (resid 58 through 103 )FF58 - 10355 - 100
2626chain 'F' and (resid 104 through 162 )FF104 - 162101 - 156
2727chain 'F' and (resid 163 through 191 )FF163 - 191157 - 185
2828chain 'F' and (resid 192 through 222 )FF192 - 222186 - 209
2929chain 'F' and (resid 223 through 274 )FF223 - 274210 - 250
3030chain 'F' and (resid 275 through 286 )FF275 - 286251 - 262

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