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Open data
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Basic information
Entry | Database: PDB / ID: 8bux | |||||||||
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Title | Rab-binding domain of human MiniBAR | |||||||||
![]() | Granule associated Rac and RHOG effector protein 1 | |||||||||
![]() | PROTEIN TRANSPORT / Rac effector / Rab effector | |||||||||
Function / homology | ![]() CCR4-NOT complex binding / Rac protein signal transduction / RHOG GTPase cycle / RAC2 GTPase cycle / RAC3 GTPase cycle / RAC1 GTPase cycle / P-body / small GTPase binding / cytosol Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Pylypenko, O. / Hammich, H. / Houdusse, A. | |||||||||
Funding support | ![]()
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![]() | ![]() Title: MiniBAR/KIAA0355 is a dual Rac and Rab effector that controls actin contractility and trafficking for successful ciliogenesis Authors: Shaughnessy, R. / Serres, M. / Escot, S. / Hammich, H. / Cuvelier, F. / Rocancourt, M. / Verdon, Q. / Gaffuri, A. / Sourigues, Y. / Malherbe, G. / Velikovsky, L. / Chardon, F. / Thinevez, J. ...Authors: Shaughnessy, R. / Serres, M. / Escot, S. / Hammich, H. / Cuvelier, F. / Rocancourt, M. / Verdon, Q. / Gaffuri, A. / Sourigues, Y. / Malherbe, G. / Velikovsky, L. / Chardon, F. / Thinevez, J. / Callebaut, I. / Formstecher, E. / Houdusse, A. / David, N. / Pylypenko, O. / Echard, A. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 213.8 KB | Display | ![]() |
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PDB format | ![]() | 142.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 8buyC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 36600.695 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||||
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#2: Chemical | ChemComp-EDO / | ||||||
#3: Chemical | #4: Chemical | ChemComp-MG / | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.95 Å3/Da / Density % sol: 36.81 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, hanging drop / Details: PEG |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jun 15, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97911 Å / Relative weight: 1 |
Reflection | Resolution: 1.856→28.58 Å / Num. obs: 24856 / % possible obs: 99.5 % / Redundancy: 3.6 % / Biso Wilson estimate: 24.06 Å2 / CC1/2: 0.996 / Net I/σ(I): 10.6 |
Reflection shell | Resolution: 1.856→1.888 Å / Num. unique obs: 4668 / CC1/2: 0.849 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 34.67 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.86→28.58 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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