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- PDB-8bh5: SARS-CoV-2 BA.2.12.1 RBD in complex with Beta-27 Fab and C1 nanobody -

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ID or keywords:

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Basic information

Entry
Database: PDB / ID: 8bh5
TitleSARS-CoV-2 BA.2.12.1 RBD in complex with Beta-27 Fab and C1 nanobody
Components
  • Beta-27 heavy chain
  • Beta-27 light chain
  • Nanobody C1
  • Spike protein S1
KeywordsIMMUNE SYSTEM / Viral Protein/Immune system / SARS-CoV-2 / Omicron / BA.2.12.1 / RBD / Beta-27
Function / homology
Function and homology information


symbiont-mediated disruption of host tissue / Maturation of spike protein / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / viral translation / host extracellular space / symbiont-mediated-mediated suppression of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion ...symbiont-mediated disruption of host tissue / Maturation of spike protein / Translation of Structural Proteins / Virion Assembly and Release / host cell surface / viral translation / host extracellular space / symbiont-mediated-mediated suppression of host tetherin activity / Induction of Cell-Cell Fusion / structural constituent of virion / membrane fusion / entry receptor-mediated virion attachment to host cell / Attachment and Entry / host cell endoplasmic reticulum-Golgi intermediate compartment membrane / positive regulation of viral entry into host cell / receptor-mediated virion attachment to host cell / host cell surface receptor binding / symbiont-mediated suppression of host innate immune response / receptor ligand activity / endocytosis involved in viral entry into host cell / fusion of virus membrane with host plasma membrane / fusion of virus membrane with host endosome membrane / viral envelope / symbiont entry into host cell / virion attachment to host cell / SARS-CoV-2 activates/modulates innate and adaptive immune responses / host cell plasma membrane / virion membrane / identical protein binding / membrane / plasma membrane
Similarity search - Function
Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal / Spike glycoprotein, N-terminal domain superfamily / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Spike glycoprotein, betacoronavirus / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus ...Spike (S) protein S1 subunit, receptor-binding domain, SARS-CoV-2 / Spike (S) protein S1 subunit, N-terminal domain, SARS-CoV-like / Coronavirus spike glycoprotein S1, C-terminal / Coronavirus spike glycoprotein S1, C-terminal / Spike glycoprotein, N-terminal domain superfamily / Spike S1 subunit, receptor binding domain superfamily, betacoronavirus / Spike glycoprotein, betacoronavirus / Betacoronavirus spike (S) glycoprotein S1 subunit N-terminal (NTD) domain profile. / Betacoronavirus spike (S) glycoprotein S1 subunit C-terminal (CTD) domain profile. / Spike (S) protein S1 subunit, receptor-binding domain, betacoronavirus / Betacoronavirus spike glycoprotein S1, receptor binding / Spike glycoprotein S1, N-terminal domain, betacoronavirus-like / Betacoronavirus-like spike glycoprotein S1, N-terminal / Spike glycoprotein S2 superfamily, coronavirus / Spike glycoprotein S2, coronavirus, heptad repeat 1 / Spike glycoprotein S2, coronavirus, heptad repeat 2 / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 1 (HR1) region profile. / Coronavirus spike (S) glycoprotein S2 subunit heptad repeat 2 (HR2) region profile. / Spike glycoprotein S2, coronavirus / Coronavirus spike glycoprotein S2 / Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta
Similarity search - Domain/homology
PHOSPHATE ION / Spike glycoprotein
Similarity search - Component
Biological speciesHomo sapiens (human)
Severe acute respiratory syndrome coronavirus 2
Lama glama (llama)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.38 Å
AuthorsHuo, J. / Zhou, D. / Ren, J. / Stuart, D.I.
Funding support United Kingdom, 2items
OrganizationGrant numberCountry
CAMS Innovation Fund for Medical Sciences (CIFMS)2018-I2M-2-002 United Kingdom
Medical Research Council (MRC, United Kingdom)MR/N00065X/1 United Kingdom
CitationJournal: Cell Discov / Year: 2022
Title: Humoral responses against SARS-CoV-2 Omicron BA.2.11, BA.2.12.1 and BA.2.13 from vaccine and BA.1 serum.
Authors: Huo, J. / Dijokaite-Guraliuc, A. / Nutalai, R. / Das, R. / Zhou, D. / Mentzer, A.J. / Fry, E.E. / Mongkolsapaya, J. / Ren, J. / Stuart, D.I. / Screaton, G.R.
History
DepositionOct 29, 2022Deposition site: PDBE / Processing site: PDBE
Revision 1.0Nov 23, 2022Provider: repository / Type: Initial release
Revision 1.1Jan 31, 2024Group: Data collection / Refinement description
Category: chem_comp_atom / chem_comp_bond / pdbx_initial_refinement_model
Revision 1.2Nov 13, 2024Group: Structure summary / Category: pdbx_entry_details / pdbx_modification_feature / Item: _pdbx_entry_details.has_protein_modification

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
H: Beta-27 heavy chain
L: Beta-27 light chain
E: Spike protein S1
C: Nanobody C1
hetero molecules


Theoretical massNumber of molelcules
Total (without water)85,93418
Polymers84,3664
Non-polymers1,56714
Water2,846158
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: homology
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area10960 Å2
ΔGint-32 kcal/mol
Surface area32230 Å2
MethodPISA
Unit cell
Length a, b, c (Å)186.846, 99.987, 56.486
Angle α, β, γ (deg.)90.000, 104.105, 90.000
Int Tables number5
Space group name H-MC121
Space group name HallC2y
Symmetry operation#1: x,y,z
#2: -x,y,-z
#3: x+1/2,y+1/2,z
#4: -x+1/2,y+1/2,-z

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Components

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Antibody , 3 types, 3 molecules HLC

#1: Antibody Beta-27 heavy chain


Mass: 23532.479 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#2: Antibody Beta-27 light chain


Mass: 23129.549 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Production host: Homo sapiens (human)
#4: Antibody Nanobody C1


Mass: 14659.114 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Lama glama (llama) / Production host: Escherichia coli (E. coli)

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Protein / Sugars , 2 types, 2 molecules E

#3: Protein Spike protein S1


Mass: 23044.971 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Severe acute respiratory syndrome coronavirus 2
Gene: S, 2 / Production host: Homo sapiens (human) / References: UniProt: P0DTC2
#5: Polysaccharide alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose


Type: oligosaccharide / Mass: 367.349 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
DescriptorTypeProgram
LFucpa1-6DGlcpNAcb1-Glycam Condensed SequenceGMML 1.0
WURCS=2.0/2,2,1/[a2122h-1b_1-5_2*NCC/3=O][a1221m-1a_1-5]/1-2/a6-b1WURCSPDB2Glycan 1.1.0
[]{[(4+1)][b-D-GlcpNAc]{[(6+1)][a-L-Fucp]{}}}LINUCSPDB-CARE

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Non-polymers , 3 types, 171 molecules

#6: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: C3H8O3
#7: Chemical ChemComp-PO4 / PHOSPHATE ION


Mass: 94.971 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: PO4
#8: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 158 / Source method: isolated from a natural source / Formula: H2O

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Details

Has ligand of interestN
Has protein modificationY

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.03 Å3/Da / Density % sol: 59.44 %
Crystal growTemperature: 293 K / Method: vapor diffusion, sitting drop
Details: 0.1 M Sodium citrate tribasic dehydrate, pH 5.5, 18% w/v Polyethylene glycol 3,350

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Data collection

DiffractionMean temperature: 100 K / Serial crystal experiment: N
Diffraction sourceSource: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.9763 Å
DetectorType: DECTRIS EIGER2 XE 16M / Detector: PIXEL / Date: May 31, 2022
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9763 Å / Relative weight: 1
ReflectionResolution: 2.38→55 Å / Num. obs: 38493 / % possible obs: 94.8 % / Redundancy: 11.2 % / Biso Wilson estimate: 48.2 Å2 / CC1/2: 0.988 / Rmerge(I) obs: 0.24 / Rpim(I) all: 0.071 / Net I/σ(I): 6.3
Reflection shellResolution: 2.38→2.42 Å / Mean I/σ(I) obs: 0.3 / Num. unique obs: 1396 / CC1/2: 0.13

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Processing

Software
NameVersionClassification
GDA1.19_4092data collection
PHENIX1.19_4092refinement
xia2data reduction
xia2data scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 7zxu

7zxu


Resolution: 2.38→54.78 Å / SU ML: 0.3416 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 24.4156
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
RfactorNum. reflection% reflection
Rfree0.2325 1842 4.97 %
Rwork0.1857 35221 -
obs0.188 37063 91.35 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL
Displacement parametersBiso mean: 57.92 Å2
Refinement stepCycle: LAST / Resolution: 2.38→54.78 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms5705 0 101 158 5964
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00185953
X-RAY DIFFRACTIONf_angle_d0.49838075
X-RAY DIFFRACTIONf_chiral_restr0.0415881
X-RAY DIFFRACTIONf_plane_restr0.00411034
X-RAY DIFFRACTIONf_dihedral_angle_d11.77532131
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.38-2.440.3754600.34021535X-RAY DIFFRACTION51.42
2.44-2.510.3589990.32312099X-RAY DIFFRACTION70.86
2.51-2.60.36471350.30972466X-RAY DIFFRACTION82.86
2.6-2.690.32311600.2872570X-RAY DIFFRACTION88.41
2.69-2.80.29021480.24312782X-RAY DIFFRACTION93.94
2.8-2.920.28181550.22942915X-RAY DIFFRACTION99.64
2.92-3.080.24251750.21262931X-RAY DIFFRACTION100
3.08-3.270.27581370.20572992X-RAY DIFFRACTION100
3.27-3.520.22481530.18132990X-RAY DIFFRACTION99.97
3.52-3.880.21111400.17242948X-RAY DIFFRACTION100
3.88-4.440.17951780.14662960X-RAY DIFFRACTION100
4.44-5.590.21281410.13363006X-RAY DIFFRACTION100
5.59-54.780.2071610.16983027X-RAY DIFFRACTION99.91
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.81747013823-0.84302157048-0.7222111569942.6453389221.461974952672.71578112217-0.0598355461695-0.0838667258035-0.1112790022610.123635261516-0.03858593544930.0197694298890.1977374751240.1292044488210.113694566740.3813949229270.02831225198580.0004502802393270.3611346335770.02307529918980.33080539375438.7990614018-38.488771899933.6745675448
21.593562862820.584218320471-0.05724818666191.538218340640.8177875155821.75687226288-0.382081284855-0.1072587228740.167247707260.1104549853190.2441177265920.268072078117-0.148863572738-0.4130347099650.0807504721050.482833477316-0.04997195712010.00716628236880.5564736752590.1159209612030.39364489535310.1068840576-22.160373241845.3147307582
35.499285416060.4412803411461.835796862752.790963965720.5721116909953.567436608190.122493232117-0.283587631771-0.6214291875640.2079909146890.1594236975460.248915235380.183737123659-0.379836456934-0.2360164401170.287007685661-0.0149827765001-0.00973653432180.3283863722080.06190958099070.3890925153245.83983293358-27.685018960642.6131874331
49.392973327490.182821101484-1.468031770614.20411278513-1.734779415932.816731595060.4058218287960.5117082794210.299159816746-0.258799070835-0.285059431858-0.210971591609-0.198196976983-0.0679034689361-0.08426930655360.482232776070.152039164798-0.002176625543590.485441243647-0.04243586826490.35932521798529.7582120889-27.828950333211.6960387613
53.27346477687-0.986919252707-0.1904706235613.431345306360.2208404825263.10478336451-0.0163473331350.320073412347-0.368090819041-0.3096176152620.07073745353030.1382413484530.233849126177-0.0346664800136-0.03358601788720.4220918812720.0334198742929-0.06374027574730.47433820986-0.05598685405090.39824933212429.1738165804-40.583075946816.0199824718
62.96507720405-1.35193621181-0.8343961972251.903573150910.9466108753870.5235625002240.01417509427970.291532187145-0.272761956332-0.0212506298949-0.1035920571440.182624505796-0.0085674486368-0.03647612906090.09711188490680.3273468324430.0178759862192-0.05453895521730.3598198495680.03564292113710.28130901582818.5976695655-26.870968581223.6036128083
73.53383186170.7604650600641.027310177651.955399365922.353726925712.89426226863-0.03566080179740.1730577876960.320208171072-0.218460467574-0.001916079608080.220925662868-0.4763658365630.210594559242-0.07431029331280.400596870352-0.04655914281550.04604381195790.4178636938420.05144873270350.46859721517211.2744683521-12.029107043330.5579637769
82.93744550541-0.747412463564-0.3931834662754.139000806481.712643995565.289739214350.0719419151077-0.05989084764940.08508383380350.02718007584540.09030084338820.167189273384-0.0797282759702-0.202598830496-0.1411865217610.291813682155-0.0263036229455-0.02841992893950.3421319235470.04678264917090.389175273345.80511478634-12.975364542533.8862975433
98.42802443030.843415759574-0.641791186184.50440716628-0.8436890450865.54354856368-0.4017391951810.9111428891860.197907802918-0.881188610178-0.00804432076607-1.36159144048-0.2923513140491.080189710170.1399025959040.6941000725930.1318430855550.2247934615550.7521449209190.1252512548420.83350348288673.5562159723-38.12376959544.0919992937
103.309227854162.573961883390.324941955098.07950315733-0.3939915598645.524813692210.3321025906980.837048551905-0.0522257291020.02751760727430.015191086194-1.526816541760.05513087699171.05641872535-0.3058295094360.483911387290.02617975444550.09395905609490.6071922084890.07082493257240.7758866188574.1941903055-42.184037033311.675562718
112.94328144579-1.29014338271-1.085361920172.00517860568-1.260667491747.468309102060.713085374249-0.01361390982841.045195968430.07852421474-1.25929525581-1.46538467379-1.091037191750.9634434725360.5189828215050.884082684112-0.1288976311270.0579805389080.7498455762220.1623303847541.0540696651772.7291993945-25.30876263919.50997699133
121.3404489491-2.3831307466-0.8422736245156.13709119287-2.052236129937.781320306160.455911511201-0.076573245964-0.786243909180.9685501334540.299070758223-2.49833701033-0.3382162885821.11324504376-0.6125946512150.615438145954-0.0563094173469-0.2208196106540.627722975657-0.1039319110081.3102878617477.8441582853-29.364251272219.3538637005
131.792388728920.833726052307-1.044907337154.86483630420.02406354373975.813163291710.129247709567-0.03663190424690.2526447878740.213338916911-0.0313323959364-0.440521850759-0.0657536388134-0.14080653507-0.1263543542860.3494328802280.0266897007893-0.00350611562480.3318488192690.01325183270790.38354013949961.287065134-37.852174393617.9947706759
143.58404948236-0.163007481756-0.2206740129743.51527248504-0.24135223261.69265099468-0.03585698907490.0703184827467-0.0519685325019-0.11001254282-0.077367297548-0.3749645167350.03356274385450.1854676782290.1143185721880.2892767425680.05615357591380.02857819958070.3193743183340.03571268757170.27229620999258.8113954431-39.428125666812.7663641519
153.568389047970.160874442299-1.053770148972.765543535411.895828264672.83816676528-0.247589411448-0.76234013106-0.4946068151230.280027576897-0.222709238858-0.267838917950.127562399280.4570505323280.5045207042060.3902777960920.0909660037345-0.102675590250.4289692499780.05140599287040.38196178046962.8537450967-48.763967035520.3599536882
163.190472459271.6067083277-0.8859361659457.59564544334-2.025429055878.86496797078-0.426262386866-0.208823751665-1.382938145670.5382617906740.2038066626630.4298278646790.918608049006-0.2307652831330.2201972030980.60336620231-0.02097861194860.06967701248490.355160467290.03917634589610.66210681295245.8654189695-57.644834396322.1767491347
172.022633645950.541917812342-0.5196826926221.54871236671-1.077365278550.921222282227-0.2558293966690.284417622526-0.2787026767-0.7228505170730.205324891682-0.1418460634070.406555760871-0.09701950773420.1616297788220.4779352227520.05067623671090.06897535660740.428942803624-0.03129833165580.39075595320846.9949906192-44.784606682811.4521245608
180.681659687511-0.03132331234890.5443528424055.93821243104-0.2592096747138.059744984790.07075844645010.67910414480.373588055620.503926453814-0.212088800991-1.3773024275-0.3482872956981.062759721940.0183287194790.421254336972-0.050303883437-0.004900293164750.4630422649250.05676207133250.69839027131671.9082473667-36.170457877514.4196900434
197.81484591663-2.53184313560.8178973346932.0558571018-2.974954720757.75945764457-0.283669554223-1.347293164010.5071065652491.487984521660.410455733581-0.0727456326212-1.08655235983-0.513225835253-0.004207354566110.884866018569-0.0365721774522-0.06135405119560.586827102386-0.1522384192870.68135066942159.5390287229-11.960136103831.3411074409
202.011234587880.687003372661-2.493227646990.9292093286280.6385677239472.76647747107-0.428543807240.3656239312221.58730634193-0.5141133574680.0633719751015-1.50667180093-1.85115183449-0.5212515411290.2293919524450.7680456733680.02395431925760.2126426482290.5970938611390.08177963465540.9486737560956.41925645472.9037751569712.3540851635
212.97735586523-2.658244581130.2731580798054.76602476193-0.9265326944513.180088719560.135738538523-0.3638140461450.6801039468071.16579836079-0.209547592922-1.146795822280.1265620812260.657148165026-0.06951018005680.823639404890.0297014875241-0.2002088754490.514848834658-0.1868878197550.89768834957466.7286870603-13.760706418528.1823869682
228.60095562037-4.16110719483-0.7803821123667.72140003643-2.010192461584.382376687830.2654946229040.433950394072-0.1874119092420.465825007617-0.00460342438981-0.0343679045257-0.196999617487-0.0163450097816-0.2304184012810.523005454549-0.01932387240340.05423837326760.381672313635-0.07868371924450.59294348325657.9528691285-15.496152839116.0808838604
236.28889221056-1.590003497550.8368868120585.98544202885-0.7811051194996.679515556950.0822516288609-0.2455528355960.4522749588450.286466886814-0.0411795635144-1.66161765015-0.2256758547041.06746745313-0.3796181769630.390384469099-0.0564224874214-0.1082043903710.50656698857-0.1498842035870.89179915784472.9094386726-16.268877716418.1079433917
247.34217085473-1.957867323310.9148497290797.24239221610.164758291455.087168904240.2045616432520.774810163721.04693049544-0.3405903675750.128850939116-1.70254175909-0.6609317141510.845682019965-0.3373768997780.554031529117-0.02900540294870.1927471288730.4695020663010.0599032640670.85639411915567.5046720984-9.2130389511313.2049311323
253.9559664252-2.59640448945-1.309367045536.13852899594-0.8064209800742.818802512210.3425105204-0.02870359564640.7792675678010.110751943278-0.182874761812-1.07598594019-0.1942508366090.519943182076-0.03515395841150.613465122743-0.0806115212530.0299434068010.468432644156-0.0458755329530.75879267269661.5423676683-7.5594398036718.2121442736
266.03296085893-1.77035684551-0.5625672172135.739558628070.3026753098973.73520363418-0.272673574999-0.4991924961410.3374428010951.118925291310.225768077337-0.1607247138590.526347543591-0.09589677959090.07241496563050.56420100060.04377433306940.01981115712010.310006325454-0.01487423822690.44129964204260.3298356434-21.869591373322.1980558602
274.091745724420.0775229783559-4.03309322682.980950820483.3219085897.848997457730.5861714390021.18394865370.001905049773080.3108050024850.159817692479-0.490607771337-1.25373145638-0.592943159006-0.663685053380.6414732374710.07363300512230.05312841556170.4296648990940.0938490887070.5830011409551.7136340388-2.1133890739613.7734366586
Refinement TLS group

Refine-ID: X-RAY DIFFRACTION

IDRefine TLS-IDSelection detailsAuth asym-IDLabel asym-IDAuth seq-IDLabel seq-ID
11chain 'H' and (resid 1 through 113 )HA1 - 1131 - 113
22chain 'H' and (resid 114 through 138 )HA114 - 138114 - 136
33chain 'H' and (resid 139 through 220 )HA139 - 220137 - 218
44chain 'L' and (resid 1 through 30 )LF1 - 301 - 30
55chain 'L' and (resid 31 through 62 )LF31 - 6231 - 62
66chain 'L' and (resid 63 through 137 )LF63 - 13763 - 137
77chain 'L' and (resid 138 through 163 )LF138 - 163138 - 163
88chain 'L' and (resid 164 through 213 )LF164 - 213164 - 213
99chain 'E' and (resid 333 through 349 )EJ333 - 3491 - 17
1010chain 'E' and (resid 350 through 364 )EJ350 - 36418 - 32
1111chain 'E' and (resid 365 through 375 )EJ365 - 37533 - 43
1212chain 'E' and (resid 376 through 393 )EJ376 - 39344 - 61
1313chain 'E' and (resid 394 through 421 )EJ394 - 42162 - 89
1414chain 'E' and (resid 422 through 459 )EJ422 - 45990 - 127
1515chain 'E' and (resid 460 through 469 )EJ460 - 469128 - 137
1616chain 'E' and (resid 470 through 479 )EJ470 - 479138 - 147
1717chain 'E' and (resid 480 through 506 )EJ480 - 506148 - 174
1818chain 'E' and (resid 507 through 525 )EJ507 - 525175 - 187
1919chain 'C' and (resid 1 through 8 )CQ1 - 81 - 8
2020chain 'C' and (resid 9 through 17 )CQ9 - 179 - 17
2121chain 'C' and (resid 18 through 32 )CQ18 - 3218 - 32
2222chain 'C' and (resid 33 through 52 )CQ33 - 5233 - 52
2323chain 'C' and (resid 53 through 60 )CQ53 - 6053 - 60
2424chain 'C' and (resid 61 through 73 )CQ61 - 7361 - 73
2525chain 'C' and (resid 74 through 99 )CQ74 - 9974 - 99
2626chain 'C' and (resid 100 through 117 )CQ100 - 117100 - 117
2727chain 'C' and (resid 118 through 124 )CQ118 - 124118 - 124

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