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- PDB-8bal: Niako3494, a bacterial protein structure in glycoside hydrolase f... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8bal | ||||||||||||
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Title | Niako3494, a bacterial protein structure in glycoside hydrolase family 20 | ||||||||||||
![]() | Beta-N-acetylhexosaminidase | ||||||||||||
![]() | HYDROLASE / GH20 apo protein | ||||||||||||
Function / homology | Glycoside hydrolase family 20, catalytic domain / Glycosyl hydrolase family 20, catalytic domain / beta-N-acetylhexosaminidase activity / N-acetyl-beta-D-galactosaminidase activity / Glycoside hydrolase superfamily / carbohydrate metabolic process / Glycoside hydrolase, family 20, catalytic core![]() | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Dong, M.D. / Roth, C.R. / Jin, Y.J. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Mechanistic and Structural Insights into the Specificity and Biological Functions of Bacterial Sulfoglycosidases Authors: Zhang, Z. / Dong, M. / Zallot, R. / Blackburn, G.M. / Wang, N. / Wang, C. / Chen, L. / Baumann, P. / Wu, Z. / Wang, Z. / Fan, H. / Roth, C. / Jin, Y. / He, Y. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 352.4 KB | Display | ![]() |
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PDB format | ![]() | 276.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 703.3 KB | Display | ![]() |
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Full document | ![]() | 807.8 KB | Display | |
Data in XML | ![]() | 71.4 KB | Display | |
Data in CIF | ![]() | 96.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 7dupC ![]() 7dvaC ![]() 7dvbC ![]() 8bblC ![]() 8bdpC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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5 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 43543.293 Da / Num. of mol.: 5 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-ZN / #3: Water | ChemComp-HOH / | Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3 Å3/Da / Density % sol: 58.98 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: Protein stock at 18mg/mL, in the precipitant: 0.1 M MMT buffer (DL-Malic acid, MES monohydrate, Tris base [ratio 1:2:2]), pH 6.0, 25 % w/v PEG 1500, protein to precipitant ratio 1:1, 1 uL ...Details: Protein stock at 18mg/mL, in the precipitant: 0.1 M MMT buffer (DL-Malic acid, MES monohydrate, Tris base [ratio 1:2:2]), pH 6.0, 25 % w/v PEG 1500, protein to precipitant ratio 1:1, 1 uL drop size, +50 nL 1:10000 diluted seed stock. Cryoprotectant used was made up with 20% PEG400 in 80% mother liquor. |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | ||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||||||||||||||
Detector | Type: DECTRIS EIGER2 XE 16M / Detector: PIXEL / Date: Apr 29, 2022 | ||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||||||||||||||
Radiation wavelength | Wavelength: 0.9762 Å / Relative weight: 1 | ||||||||||||||||||||||||
Reflection twin |
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Reflection | Resolution: 2.27→73.49 Å / Num. obs: 120442 / % possible obs: 99.9 % / Redundancy: 13.9 % / CC1/2: 0.958 / Net I/σ(I): 6.3 | ||||||||||||||||||||||||
Reflection shell | Resolution: 2.27→2.31 Å / Redundancy: 13.6 % / Mean I/σ(I) obs: 1.1 / Num. unique obs: 5852 / CC1/2: 0.336 / % possible all: 99.1 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: Alphafold Resolution: 2.27→73.49 Å / Cor.coef. Fo:Fc: 0.878 / Cor.coef. Fo:Fc free: 0.811 / SU B: 5.491 / SU ML: 0.148 / Cross valid method: FREE R-VALUE / ESU R: 0.067 / ESU R Free: 0.06 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.39 Å2
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Refinement step | Cycle: LAST / Resolution: 2.27→73.49 Å
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Refine LS restraints |
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LS refinement shell |
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