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- PDB-8arn: Crystal structure of the peptide binding protein, OppA, from Baci... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8arn | ||||||
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Title | Crystal structure of the peptide binding protein, OppA, from Bacillus subtilis in complex with an endogenous tetrapeptide | ||||||
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![]() | TRANSPORT PROTEIN / Peptide-binding protein / Sporulation Bacillus subtilis | ||||||
Function / homology | ![]() establishment of competence for transformation / peptide transport / peptide transmembrane transporter activity / sporulation resulting in formation of a cellular spore / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / protein transport / outer membrane-bounded periplasmic space / membrane raft Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hughes, A. / Dodson, E.J. / Wilkinson, A.J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Peptide transport in Bacillus subtilis - structure and specificity in the extracellular solute binding proteins OppA and DppE. Authors: Hughes, A.M. / Darby, J.F. / Dodson, E.J. / Wilson, S.J. / Turkenburg, J.P. / Thomas, G.H. / Wilkinson, A.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 417.7 KB | Display | ![]() |
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PDB format | ![]() | 329.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 439.7 KB | Display | ![]() |
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Full document | ![]() | 445.2 KB | Display | |
Data in XML | ![]() | 44.6 KB | Display | |
Data in CIF | ![]() | 67.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8areC ![]() 8ay0C ![]() 8azbC ![]() 1rkmS S: Starting model for refinement C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 59231.293 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Recombinant protein purified from E. coli with a co-purifying tetrapeptide ligand SNSS Source: (gene. exp.) ![]() ![]() Strain: 168 / Gene: oppA, spo0KA, BSU11430 / Production host: ![]() ![]() #2: Protein/peptide | Mass: 393.351 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: Peptide co-purifying with the recombinant protein and bound in the peptide binding cleft Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.72 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop Details: Crystals of OppA were obtained from hanging drops composed of 2 microlitres of reservoir solution consisting of 0.1 M MMT, 22.5% PEG 1500 and 2.5% DMSO pH 8.0 and 2 microlitres protein at 18 ...Details: Crystals of OppA were obtained from hanging drops composed of 2 microlitres of reservoir solution consisting of 0.1 M MMT, 22.5% PEG 1500 and 2.5% DMSO pH 8.0 and 2 microlitres protein at 18 mg.ml-1 with crystal optimisation following a seeding protocol. |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N | |||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||||||||
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 4, 2015 | |||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||
Radiation wavelength | Wavelength: 0.82 Å / Relative weight: 1 | |||||||||||||||||||||
Reflection | Resolution: 1.5→55.025 Å / Num. obs: 158694 / % possible obs: 99.9 % / Redundancy: 4.2 % / CC1/2: 0.998 / Rmerge(I) obs: 0.045 / Rpim(I) all: 0.038 / Rrim(I) all: 0.059 / Net I/σ(I): 12.6 | |||||||||||||||||||||
Reflection shell | Diffraction-ID: 1
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 1RKM Resolution: 1.5→55.025 Å / Cor.coef. Fo:Fc: 0.974 / Cor.coef. Fo:Fc free: 0.965 / SU B: 1.752 / SU ML: 0.063 / Cross valid method: FREE R-VALUE / ESU R: 0.076 / ESU R Free: 0.077 Details: Hydrogens have been added in their riding positions
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK BULK SOLVENT | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.694 Å2
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Refinement step | Cycle: LAST / Resolution: 1.5→55.025 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 20
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