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- PDB-8apz: Crystal structure of wild-type L-N-Carbamoylase from Sinorhizobiu... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8apz | ||||||
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Title | Crystal structure of wild-type L-N-Carbamoylase from Sinorhizobium meliloti | ||||||
![]() | N-carbamoyl-L-amino-acid hydrolase![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Rozeboom, H.J. / Mayer, C. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Selecting Better Biocatalysts by Complementing Recoded Bacteria. Authors: Rubini, R. / Jansen, S.C. / Beekhuis, H. / Rozeboom, H.J. / Mayer, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 326.8 KB | Display | ![]() |
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PDB format | ![]() | 261.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 8aq0C C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | ![]() Mass: 47039.250 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: First 21 residues are expression tag / Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: Q6DTN4, ![]() |
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-Non-polymers , 6 types, 476 molecules ![](data/chem/img/ZN.gif)
![](data/chem/img/FE.gif)
![](data/chem/img/ORD.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/FE.gif)
![](data/chem/img/ORD.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/GOL.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | ChemComp-ZN / #3: Chemical | ChemComp-FE / ![]() #4: Chemical | ![]() #5: Chemical | ![]() #6: Chemical | ![]() #7: Water | ChemComp-HOH / | ![]() |
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-Details
Has ligand of interest | N |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2 Å3/Da / Density % sol: 38 % |
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Crystal grow![]() | Temperature: 294 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: 0.2 M sodium acetate, 0.1 M Bis-tris propane and 20 % PEG3350 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2M / Detector: PIXEL / Date: Feb 22, 2022 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.75→45.53 Å / Num. obs: 74615 / % possible obs: 99.5 % / Redundancy: 6.5 % / CC1/2: 0.997 / Rmerge(I) obs: 0.088 / Rpim(I) all: 0.037 / Rrim(I) all: 0.095 / Net I/σ(I): 13.3 / Num. measured all: 482305 |
Reflection shell | Resolution: 1.75→1.78 Å / Redundancy: 6.2 % / Rmerge(I) obs: 0.778 / Num. unique obs: 3970 / CC1/2: 0.836 / Rpim(I) all: 0.332 / Rrim(I) all: 0.848 / % possible all: 97.4 |
-Phasing
Phasing![]() | Method: ![]() | |||||||||
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Phasing MR | Model details: Phaser MODE: MR_AUTO
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: AF model Resolution: 1.75→43.22 Å / Cor.coef. Fo:Fc: 0.974 / Cor.coef. Fo:Fc free: 0.959 / SU B: 4.916 / SU ML: 0.077 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R: 0.104 / ESU R Free: 0.102 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: U VALUES : WITH TLS ADDED HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.7 Å / Shrinkage radii: 0.7 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 83.18 Å2 / Biso mean: 25.558 Å2 / Biso min: 13.88 Å2
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Refinement step | Cycle: final / Resolution: 1.75→43.22 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.75→1.795 Å / Rfactor Rfree error: 0 / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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