+Open data
-Basic information
Entry | Database: PDB / ID: 8am4 | |||||||||
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Title | Cl-rsEGFP2 Long Wavelength Structure | |||||||||
Components | Green fluorescent protein | |||||||||
Keywords | FLUORESCENT PROTEIN / GFP-like protein / beta-barrel / bioluminescence | |||||||||
Function / homology | Green fluorescent protein, GFP / Green fluorescent protein-related / Green fluorescent protein / Green fluorescent protein / bioluminescence / generation of precursor metabolites and energy / Green fluorescent protein Function and homology information | |||||||||
Biological species | Aequorea victoria (jellyfish) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2.02 Å | |||||||||
Authors | Orr, C.M. / Fadini, A. / van Thor, J. | |||||||||
Funding support | United Kingdom, 2items
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Citation | Journal: J.Am.Chem.Soc. / Year: 2023 Title: Serial Femtosecond Crystallography Reveals that Photoactivation in a Fluorescent Protein Proceeds via the Hula Twist Mechanism. Authors: Fadini, A. / Hutchison, C.D.M. / Morozov, D. / Chang, J. / Maghlaoui, K. / Perrett, S. / Luo, F. / Kho, J.C.X. / Romei, M.G. / Morgan, R.M.L. / Orr, C.M. / Cordon-Preciado, V. / Fujiwara, T. ...Authors: Fadini, A. / Hutchison, C.D.M. / Morozov, D. / Chang, J. / Maghlaoui, K. / Perrett, S. / Luo, F. / Kho, J.C.X. / Romei, M.G. / Morgan, R.M.L. / Orr, C.M. / Cordon-Preciado, V. / Fujiwara, T. / Nuemket, N. / Tosha, T. / Tanaka, R. / Owada, S. / Tono, K. / Iwata, S. / Boxer, S.G. / Groenhof, G. / Nango, E. / van Thor, J.J. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8am4.cif.gz | 60.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8am4.ent.gz | 41.9 KB | Display | PDB format |
PDBx/mmJSON format | 8am4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8am4_validation.pdf.gz | 392.7 KB | Display | wwPDB validaton report |
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Full document | 8am4_full_validation.pdf.gz | 394.7 KB | Display | |
Data in XML | 8am4_validation.xml.gz | 7 KB | Display | |
Data in CIF | 8am4_validation.cif.gz | 9.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/am/8am4 ftp://data.pdbj.org/pub/pdb/validation_reports/am/8am4 | HTTPS FTP |
-Related structure data
Related structure data | 8a6gC 8a6nC 8a6oC 8a6pC 8a6qC 8a6rC 8a6sC 8a7vC 8a83C C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 28566.648 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Aequorea victoria (jellyfish) / Gene: GFP / Production host: Escherichia coli BL21(DE3) (bacteria) / References: UniProt: P42212 |
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Has ligand of interest | Y |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43.54 % / Description: 500 um x 200 um x 200 um |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 100 mM Hepes pH 8.0, 1.80 M ammonium sulphate, 20 mM NaCl |
-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation |
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Radiation wavelength |
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Reflection | Entry-ID: 8AM4
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Reflection shell | Resolution: 2.02→2.08 Å / Rmerge(I) obs: 0.112 / Num. unique obs: 571 / CC1/2: 0.987 / Rpim(I) all: 0.112 / Rrim(I) all: 0.159 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2.02→47.11 Å / Cross valid method: THROUGHOUT
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Refinement step | Cycle: LAST / Resolution: 2.02→47.11 Å
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