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- PDB-8alq: The Solution Structure of the Triple Mutant Methyl-CpG-Binding Do... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8alq | ||||||
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Title | The Solution Structure of the Triple Mutant Methyl-CpG-Binding Domain from MeCP2 that Binds to Asymmetrically Modified DNA | ||||||
![]() | Methyl-CpG-binding protein 2 | ||||||
![]() | DNA BINDING PROTEIN / hmC-mC complex / gene regulation / methyl-CpG-binding domain (MBD) of MeCP2 | ||||||
Function / homology | ![]() double-stranded methylated DNA binding / negative regulation of transcription by RNA polymerase II / nucleus Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
![]() | Singh, H. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Epigenetic CpG duplex marks probed by an evolved DNA reader via a well-tempered conformational plasticity. Authors: Singh, H. / Das, C.K. / Buchmuller, B.C. / Schafer, L.V. / Summerer, D. / Linser, R. #1: ![]() Title: Epigenetic CpG Duplex Marks Probed by an Evolved DNA Reader via a Well-Tempered Conformational Plasticity Authors: Singh, H. / Das, C.K. / Buchmuller, B.C. / Eppmann, S. / Schafer, L.V. / Summerer, D. / Linser, R. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 369.9 KB | Display | ![]() |
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PDB format | ![]() | 312.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 408.5 KB | Display | ![]() |
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Full document | ![]() | 467.4 KB | Display | |
Data in XML | ![]() | 18.1 KB | Display | |
Data in CIF | ![]() | 29.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 8ajrC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 11905.288 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 100 mM / Label: conditions_1 / pH: 6 pH* / Pressure: 1 atm / Temperature: 305 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 10 |